Exou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/12861 |
Resumo: | Texto completo: acesso restrito. p.315-321 |
| id |
UFBA-2_c8329b4aaddc91a4a9dfa8fc6c18df9a |
|---|---|
| oai_identifier_str |
oai:repositorio.ufba.br:ri/12861 |
| network_acronym_str |
UFBA-2 |
| network_name_str |
Repositório Institucional da UFBA |
| repository_id_str |
1932 |
| spelling |
Machado, Gloria Beatriz S.Assis, Maria Cristina deLeão, RobsonSaliba, Alessandra M.Silva, Maurício Costa Alves daSuassuna, José Hermógenes RoccoOliveira, Albanita V. dePlotkowski, Maria CristinaMachado, Gloria Beatriz S.Assis, Maria Cristina deLeão, RobsonSaliba, Alessandra M.Silva, Maurício Costa Alves daSuassuna, José Hermógenes RoccoOliveira, Albanita V. dePlotkowski, Maria Cristina2013-09-06T17:30:29Z20101073-2322http://www.repositorio.ufba.br/ri/handle/ri/12861v. 33, n. 3Texto completo: acesso restrito. p.315-321To address the question whether ExoU, a Pseudomonas aeruginosa cytotoxin with phospholipase A2 activity, can induce hemostatic abnormalities during the course of pneumosepsis, mice were instilled i.t. with the ExoU-producing PA103 P. aeruginosa or with a mutant obtained by deletion of the exoU gene. Control animals were instilled with sterile vehicle. To assess the role of ExoU in animal survival, mice were evaluated for 72 h. In all the other experiments, animals were studied at 24 h after infection. PA103-infected mice showed significantly higher mortality rate, lower blood leukocyte concentration, and higher platelet concentration and hematocrit than animals infected with the bacterial mutant, as well as evidences of increased vascular permeability and plasma leakage, which were confirmed by our finding of higher protein concentration in bronchoalveolar lavage fluids and by the Evans blue dye assay. Platelets from PA103-infected mice demonstrated features of activation, assessed by the flow cytometric detection of higher percentage of P-selectin expression and of platelet-derived microparticles as well as by the enzyme immunoassay detection of increased thromboxane A2 concentration in animal plasma. Histopathology of lung and kidney sections from PA103-infected mice exhibited evidences of thrombus formation that were not detected in sections of animals from the other groups. Our results demonstrate the ability of ExoU to induce vascular hyperpermeability, platelet activation, and thrombus formation during P. aeruginosa pneumosepsis, and we speculate that this ability may contribute to the reported poor outcome of patients with severe infection by ExoU-producing P. aeruginosa.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-09-05T12:08:50Z No. of bitstreams: 1 00024382-201003000-00014.pdf: 2097152 bytes, checksum: 3654cb7569250d81bde0fb5cba302d3e (MD5)Approved for entry into archive by Vilma Conceição(vilmagc@ufba.br) on 2013-09-06T17:30:28Z (GMT) No. of bitstreams: 1 00024382-201003000-00014.pdf: 2097152 bytes, checksum: 3654cb7569250d81bde0fb5cba302d3e (MD5)Made available in DSpace on 2013-09-06T17:30:29Z (GMT). No. of bitstreams: 1 00024382-201003000-00014.pdf: 2097152 bytes, checksum: 3654cb7569250d81bde0fb5cba302d3e (MD5) Previous issue date: 2010http://dx.doi.org/10.1097/SHK.0b013e3181b2b0f4reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAPlatelet activationPlatelet-derived microparticlesP-selectin expressing plateletsPneumosepsisThromboxane A2Thrombus formationVascular hyperpermeabilityExou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsisShockinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12861/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ORIGINAL00024382-201003000-00014.pdf00024382-201003000-00014.pdfapplication/pdf2097152https://repositorio.ufba.br/bitstream/ri/12861/1/00024382-201003000-00014.pdf3654cb7569250d81bde0fb5cba302d3eMD51TEXT00024382-201003000-00014.pdf.txt00024382-201003000-00014.pdf.txtExtracted texttext/plain38225https://repositorio.ufba.br/bitstream/ri/12861/3/00024382-201003000-00014.pdf.txt3ec6a0b30c9112411c55089ec617895aMD53ri/128612022-07-05 14:03:11.297oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:11Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Exou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsis |
| dc.title.alternative.pt_BR.fl_str_mv |
Shock |
| title |
Exou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsis |
| spellingShingle |
Exou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsis Machado, Gloria Beatriz S. Platelet activation Platelet-derived microparticles P-selectin expressing platelets Pneumosepsis Thromboxane A2 Thrombus formation Vascular hyperpermeability |
| title_short |
Exou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsis |
| title_full |
Exou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsis |
| title_fullStr |
Exou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsis |
| title_full_unstemmed |
Exou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsis |
| title_sort |
Exou-induced vascular hyperpermeability and platelet activation in the course of experimental pseudomonas aeruginosa pneumosepsis |
| author |
Machado, Gloria Beatriz S. |
| author_facet |
Machado, Gloria Beatriz S. Assis, Maria Cristina de Leão, Robson Saliba, Alessandra M. Silva, Maurício Costa Alves da Suassuna, José Hermógenes Rocco Oliveira, Albanita V. de Plotkowski, Maria Cristina |
| author_role |
author |
| author2 |
Assis, Maria Cristina de Leão, Robson Saliba, Alessandra M. Silva, Maurício Costa Alves da Suassuna, José Hermógenes Rocco Oliveira, Albanita V. de Plotkowski, Maria Cristina |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Machado, Gloria Beatriz S. Assis, Maria Cristina de Leão, Robson Saliba, Alessandra M. Silva, Maurício Costa Alves da Suassuna, José Hermógenes Rocco Oliveira, Albanita V. de Plotkowski, Maria Cristina Machado, Gloria Beatriz S. Assis, Maria Cristina de Leão, Robson Saliba, Alessandra M. Silva, Maurício Costa Alves da Suassuna, José Hermógenes Rocco Oliveira, Albanita V. de Plotkowski, Maria Cristina |
| dc.subject.por.fl_str_mv |
Platelet activation Platelet-derived microparticles P-selectin expressing platelets Pneumosepsis Thromboxane A2 Thrombus formation Vascular hyperpermeability |
| topic |
Platelet activation Platelet-derived microparticles P-selectin expressing platelets Pneumosepsis Thromboxane A2 Thrombus formation Vascular hyperpermeability |
| description |
Texto completo: acesso restrito. p.315-321 |
| publishDate |
2010 |
| dc.date.issued.fl_str_mv |
2010 |
| dc.date.accessioned.fl_str_mv |
2013-09-06T17:30:29Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/12861 |
| dc.identifier.issn.none.fl_str_mv |
1073-2322 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 33, n. 3 |
| identifier_str_mv |
1073-2322 v. 33, n. 3 |
| url |
http://www.repositorio.ufba.br/ri/handle/ri/12861 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.source.pt_BR.fl_str_mv |
http://dx.doi.org/10.1097/SHK.0b013e3181b2b0f4 |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
| instname_str |
Universidade Federal da Bahia (UFBA) |
| instacron_str |
UFBA |
| institution |
UFBA |
| reponame_str |
Repositório Institucional da UFBA |
| collection |
Repositório Institucional da UFBA |
| bitstream.url.fl_str_mv |
https://repositorio.ufba.br/bitstream/ri/12861/2/license.txt https://repositorio.ufba.br/bitstream/ri/12861/1/00024382-201003000-00014.pdf https://repositorio.ufba.br/bitstream/ri/12861/3/00024382-201003000-00014.pdf.txt |
| bitstream.checksum.fl_str_mv |
1b89a9a0548218172d7c829f87a0eab9 3654cb7569250d81bde0fb5cba302d3e 3ec6a0b30c9112411c55089ec617895a |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA) |
| repository.mail.fl_str_mv |
|
| _version_ |
1808459457979482112 |