Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria

Detalhes bibliográficos
Autor(a) principal: Santos, João Conrado Khouri dos
Data de Publicação: 2020
Outros Autores: Silva Filho, João Luiz, Judice, Carla C, Kayano, Ana Carolina Andrade Vitor, Aliberti, Júlio, Cunha, Antonio Ricardo Khouri, Lima, Diógenes S. de, Nakaya, Helder, Lacerda, Marcus Vinicius Guimarães, Paula, Erich Vinicius de, Lopes, Stefanie Costa Pinto, Costa, Fabio Trindade Maranhão
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43078
Resumo: FAPESP grants #2012/16525-2 and #2017/18611-7. JCKS receives a scholarship from CAPES. JLSF receives a scholarship from FAPESP #2916/12855-9. HN, MVGL, EVP and FTMC are CNPq research fellows.
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spelling Santos, João Conrado Khouri dosSilva Filho, João LuizJudice, Carla CKayano, Ana Carolina Andrade VitorAliberti, JúlioCunha, Antonio Ricardo KhouriLima, Diógenes S. deNakaya, HelderLacerda, Marcus Vinicius GuimarãesPaula, Erich Vinicius deLopes, Stefanie Costa PintoCosta, Fabio Trindade Maranhão2020-08-31T14:33:54Z2020-08-31T14:33:54Z2020SANTOS, João Conrado Khouri dos et al. Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. LoS Neglected Tropical Diseases, 2020.https://www.arca.fiocruz.br/handle/icict/43078FAPESP grants #2012/16525-2 and #2017/18611-7. JCKS receives a scholarship from CAPES. JLSF receives a scholarship from FAPESP #2916/12855-9. HN, MVGL, EVP and FTMC are CNPq research fellows.Universidade Estadual de Campinas. Instituto de Biologia. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil / Universidade Estadual de Campinas Faculdade de Ciências Médicas.Campinas, SP, Brasil.Universidade Estadual de Campinas. Instituto de Biologia. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil.Universidade Estadual de Campinas. Instituto de Biologia. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil.Universidade Estadual de Campinas. Instituto de Biologia. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil.National Institute of Allergy and Infectious Diseases. Maryland, USA.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.University of Sao Paulo. School of Pharmaceutical Sciences. Department of Clinical and Toxicological Analyses. São Paulo, SP, Brazil..Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil..Universidade Estadual de Campinas. Centro de Hematologia e Hemoterapia. Hemocentro. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil..Universidade Estadual de Campinas. Instituto de Biologia. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil.Platelets drive endothelial cell activation in many diseases. However, if this occurs in Plasmodium vivax malaria is unclear. As platelets have been reported to be activated and to play a role in inflammatory response during malaria, we hypothesized that this would correlate with endothelial alterations during acute illness. Methods. We performed platelet flow cytometry of PAC-1 and P-selectin. We measured Platelet markers (CXCL4, CD40L, P-selectin, Thrombopoietin, IL-11) and endothelial markers (ICAM-1, von Willebrand Factor and E-selectin) in plasma with a multiplex-based assay. The values of each mediator were used to generate heatmaps, K-means clustering and Principal Component analysis. In addition, we determined pair-wise Pearson’s correlation coefficients to generate correlation networks. Results. Platelet counts were reduced, and mean platelet volume increased in malaria patients. The activation of circulating platelets in flow cytometry did not differ between patients and controls. CD40L levels (Median [IQ]: 517 [406-651] vs. 1029 [732- 1267] pg/mL, P=0.0001) were significantly higher in patients, while P-selectin (Median [IQ]: 17.0 [15.4-20.6] vs. 22.2 [17.6-25.7] ng/mL, P=0.0621) and CXCL4 showed a nonsignificant trend towards higher levels in patients. The network correlation approach demonstrated the correlation between markers of platelet and endothelial activation, and the heatmaps revealed a distinct pattern of activation in two subsets of P. vivax patients when compared to controls. Conclusion. platelet activation occurs in uncomplicated vivax malaria and this correlates with higher endothelial cell activation, especially in a subset of patients.engPublic Library of ScienceTrombocitopeniaTrombopoieseAtivação plaquetáriaCD40LAtivação de células endoteliaisThrombocytopeniaThrombopoiesisPlatelet activationCD40LP-selectinEndothelial cell activation.Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malariainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43078/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos, Joao Conrado Khouri dos Platelet disturbances....pdfSantos, Joao Conrado Khouri dos Platelet 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dc.title.pt_BR.fl_str_mv Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria
title Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria
spellingShingle Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria
Santos, João Conrado Khouri dos
Trombocitopenia
Trombopoiese
Ativação plaquetária
CD40L
Ativação de células endoteliais
Thrombocytopenia
Thrombopoiesis
Platelet activation
CD40L
P-selectin
Endothelial cell activation.
title_short Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria
title_full Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria
title_fullStr Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria
title_full_unstemmed Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria
title_sort Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria
author Santos, João Conrado Khouri dos
author_facet Santos, João Conrado Khouri dos
Silva Filho, João Luiz
Judice, Carla C
Kayano, Ana Carolina Andrade Vitor
Aliberti, Júlio
Cunha, Antonio Ricardo Khouri
Lima, Diógenes S. de
Nakaya, Helder
Lacerda, Marcus Vinicius Guimarães
Paula, Erich Vinicius de
Lopes, Stefanie Costa Pinto
Costa, Fabio Trindade Maranhão
author_role author
author2 Silva Filho, João Luiz
Judice, Carla C
Kayano, Ana Carolina Andrade Vitor
Aliberti, Júlio
Cunha, Antonio Ricardo Khouri
Lima, Diógenes S. de
Nakaya, Helder
Lacerda, Marcus Vinicius Guimarães
Paula, Erich Vinicius de
Lopes, Stefanie Costa Pinto
Costa, Fabio Trindade Maranhão
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, João Conrado Khouri dos
Silva Filho, João Luiz
Judice, Carla C
Kayano, Ana Carolina Andrade Vitor
Aliberti, Júlio
Cunha, Antonio Ricardo Khouri
Lima, Diógenes S. de
Nakaya, Helder
Lacerda, Marcus Vinicius Guimarães
Paula, Erich Vinicius de
Lopes, Stefanie Costa Pinto
Costa, Fabio Trindade Maranhão
dc.subject.other.pt_BR.fl_str_mv Trombocitopenia
Trombopoiese
Ativação plaquetária
CD40L
Ativação de células endoteliais
topic Trombocitopenia
Trombopoiese
Ativação plaquetária
CD40L
Ativação de células endoteliais
Thrombocytopenia
Thrombopoiesis
Platelet activation
CD40L
P-selectin
Endothelial cell activation.
dc.subject.en.pt_BR.fl_str_mv Thrombocytopenia
Thrombopoiesis
Platelet activation
CD40L
P-selectin
Endothelial cell activation.
description FAPESP grants #2012/16525-2 and #2017/18611-7. JCKS receives a scholarship from CAPES. JLSF receives a scholarship from FAPESP #2916/12855-9. HN, MVGL, EVP and FTMC are CNPq research fellows.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-08-31T14:33:54Z
dc.date.available.fl_str_mv 2020-08-31T14:33:54Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, João Conrado Khouri dos et al. Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. LoS Neglected Tropical Diseases, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43078
identifier_str_mv SANTOS, João Conrado Khouri dos et al. Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. LoS Neglected Tropical Diseases, 2020.
url https://www.arca.fiocruz.br/handle/icict/43078
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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