Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments

Detalhes bibliográficos
Autor(a) principal: Nishi, Sandra Mayumi
Data de Publicação: 2011
Outros Autores: Soares, Rodrigo Martins, Driemeier, David, Antoniassi, Nadia Aline Bobbi, Schares, Gereon, Gennari, Solange Maria, Alves Neto, Aldo Francisco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14844
Resumo: p. 135-139
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spelling Nishi, Sandra MayumiSoares, Rodrigo MartinsDriemeier, DavidAntoniassi, Nadia Aline BobbiSchares, GereonGennari, Solange MariaAlves Neto, Aldo Francisco2014-04-22T18:37:29Z2014-04-22T18:37:29Z20110022-3395http://repositorio.ufba.br/ri/handle/ri/14844v. 97, n. 1p. 135-139The aim of the present study was to evaluate the viability of Neospora caninum sporulated oocysts after various chemical and physical treatments. Bioassays in gerbils and molecular techniques (PCR-RFLP) were used for identification of the oocysts shed by experimentally infected dogs. Sporulated oocysts were purified and divided into 11 treatment groups as follows: absolute ethanol for 1 hr; 20 C for 6 hr; 4 C for 6 hr; 60 C for 1 min; 100 C for 1 min; 10% formaldehyde for 1 hr; 10% ammonia for 1 hr; 2% iodine for 1 hr; 10% sodium hypochlorite for 1 hr; 70% ethanol for 1 hr; and one group was left untreated and kept as a positive control. All chemical treatments were performed at room temperature (37 C). A total of 33 gerbils, or 3 gerbils per treatment, were used for bioassays. After treatment, the oocysts were divided into aliquots of 1,000 oocysts and orally administered to gerbils. After 63 days, the gerbils were anesthetized and killed with 0.2 ml of T61; blood and tissue samples were collected for serological (IFAT and western blotting), molecular (real-time PCR), histopathology, and immunohistochemical tests. Treatments were considered effective only if all 5 detection techniques tested negative. High temperatures at 100 C for 1 min and 10% sodium hypochlorite for 1 hr were the only treatments that met this condition, effectively inactivating all oocysts.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-04-08T15:16:56Z No. of bitstreams: 1 Sandra M. Nishi.pdf: 267044 bytes, checksum: 5b99c4c9e2b773ff398d0b808e693b46 (MD5)Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-04-22T18:37:29Z (GMT) No. of bitstreams: 1 Sandra M. Nishi.pdf: 267044 bytes, checksum: 5b99c4c9e2b773ff398d0b808e693b46 (MD5)Made available in DSpace on 2014-04-22T18:37:29Z (GMT). No. of bitstreams: 1 Sandra M. Nishi.pdf: 267044 bytes, checksum: 5b99c4c9e2b773ff398d0b808e693b46 (MD5) Previous issue date: 2011http://dx.doi.org/ 10.1645/GE-2571.1reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAViability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical TreatmentsJournal of Parasitologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINALSandra M. Nishi.pdfSandra M. Nishi.pdfapplication/pdf267044https://repositorio.ufba.br/bitstream/ri/14844/1/Sandra%20M.%20Nishi.pdf5b99c4c9e2b773ff398d0b808e693b46MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14844/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXTSandra M. Nishi.pdf.txtSandra M. 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dc.title.pt_BR.fl_str_mv Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments
dc.title.alternative.pt_BR.fl_str_mv Journal of Parasitology
title Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments
spellingShingle Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments
Nishi, Sandra Mayumi
title_short Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments
title_full Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments
title_fullStr Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments
title_full_unstemmed Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments
title_sort Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments
author Nishi, Sandra Mayumi
author_facet Nishi, Sandra Mayumi
Soares, Rodrigo Martins
Driemeier, David
Antoniassi, Nadia Aline Bobbi
Schares, Gereon
Gennari, Solange Maria
Alves Neto, Aldo Francisco
author_role author
author2 Soares, Rodrigo Martins
Driemeier, David
Antoniassi, Nadia Aline Bobbi
Schares, Gereon
Gennari, Solange Maria
Alves Neto, Aldo Francisco
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nishi, Sandra Mayumi
Soares, Rodrigo Martins
Driemeier, David
Antoniassi, Nadia Aline Bobbi
Schares, Gereon
Gennari, Solange Maria
Alves Neto, Aldo Francisco
description p. 135-139
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-04-22T18:37:29Z
dc.date.available.fl_str_mv 2014-04-22T18:37:29Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14844
dc.identifier.issn.none.fl_str_mv 0022-3395
dc.identifier.number.pt_BR.fl_str_mv v. 97, n. 1
identifier_str_mv 0022-3395
v. 97, n. 1
url http://repositorio.ufba.br/ri/handle/ri/14844
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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