Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/14844 |
Resumo: | p. 135-139 |
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Nishi, Sandra MayumiSoares, Rodrigo MartinsDriemeier, DavidAntoniassi, Nadia Aline BobbiSchares, GereonGennari, Solange MariaAlves Neto, Aldo Francisco2014-04-22T18:37:29Z2014-04-22T18:37:29Z20110022-3395http://repositorio.ufba.br/ri/handle/ri/14844v. 97, n. 1p. 135-139The aim of the present study was to evaluate the viability of Neospora caninum sporulated oocysts after various chemical and physical treatments. Bioassays in gerbils and molecular techniques (PCR-RFLP) were used for identification of the oocysts shed by experimentally infected dogs. Sporulated oocysts were purified and divided into 11 treatment groups as follows: absolute ethanol for 1 hr; 20 C for 6 hr; 4 C for 6 hr; 60 C for 1 min; 100 C for 1 min; 10% formaldehyde for 1 hr; 10% ammonia for 1 hr; 2% iodine for 1 hr; 10% sodium hypochlorite for 1 hr; 70% ethanol for 1 hr; and one group was left untreated and kept as a positive control. All chemical treatments were performed at room temperature (37 C). A total of 33 gerbils, or 3 gerbils per treatment, were used for bioassays. After treatment, the oocysts were divided into aliquots of 1,000 oocysts and orally administered to gerbils. After 63 days, the gerbils were anesthetized and killed with 0.2 ml of T61; blood and tissue samples were collected for serological (IFAT and western blotting), molecular (real-time PCR), histopathology, and immunohistochemical tests. Treatments were considered effective only if all 5 detection techniques tested negative. High temperatures at 100 C for 1 min and 10% sodium hypochlorite for 1 hr were the only treatments that met this condition, effectively inactivating all oocysts.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-04-08T15:16:56Z No. of bitstreams: 1 Sandra M. Nishi.pdf: 267044 bytes, checksum: 5b99c4c9e2b773ff398d0b808e693b46 (MD5)Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-04-22T18:37:29Z (GMT) No. of bitstreams: 1 Sandra M. Nishi.pdf: 267044 bytes, checksum: 5b99c4c9e2b773ff398d0b808e693b46 (MD5)Made available in DSpace on 2014-04-22T18:37:29Z (GMT). No. of bitstreams: 1 Sandra M. Nishi.pdf: 267044 bytes, checksum: 5b99c4c9e2b773ff398d0b808e693b46 (MD5) Previous issue date: 2011http://dx.doi.org/ 10.1645/GE-2571.1reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAViability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical TreatmentsJournal of Parasitologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINALSandra M. Nishi.pdfSandra M. Nishi.pdfapplication/pdf267044https://repositorio.ufba.br/bitstream/ri/14844/1/Sandra%20M.%20Nishi.pdf5b99c4c9e2b773ff398d0b808e693b46MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14844/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXTSandra M. Nishi.pdf.txtSandra M. 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dc.title.pt_BR.fl_str_mv |
Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments |
dc.title.alternative.pt_BR.fl_str_mv |
Journal of Parasitology |
title |
Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments |
spellingShingle |
Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments Nishi, Sandra Mayumi |
title_short |
Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments |
title_full |
Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments |
title_fullStr |
Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments |
title_full_unstemmed |
Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments |
title_sort |
Viability of Sporulated Oocysts of Neospora caninum After Exposure to Different Physical and Chemical Treatments |
author |
Nishi, Sandra Mayumi |
author_facet |
Nishi, Sandra Mayumi Soares, Rodrigo Martins Driemeier, David Antoniassi, Nadia Aline Bobbi Schares, Gereon Gennari, Solange Maria Alves Neto, Aldo Francisco |
author_role |
author |
author2 |
Soares, Rodrigo Martins Driemeier, David Antoniassi, Nadia Aline Bobbi Schares, Gereon Gennari, Solange Maria Alves Neto, Aldo Francisco |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Nishi, Sandra Mayumi Soares, Rodrigo Martins Driemeier, David Antoniassi, Nadia Aline Bobbi Schares, Gereon Gennari, Solange Maria Alves Neto, Aldo Francisco |
description |
p. 135-139 |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2014-04-22T18:37:29Z |
dc.date.available.fl_str_mv |
2014-04-22T18:37:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufba.br/ri/handle/ri/14844 |
dc.identifier.issn.none.fl_str_mv |
0022-3395 |
dc.identifier.number.pt_BR.fl_str_mv |
v. 97, n. 1 |
identifier_str_mv |
0022-3395 v. 97, n. 1 |
url |
http://repositorio.ufba.br/ri/handle/ri/14844 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.source.pt_BR.fl_str_mv |
http://dx.doi.org/ 10.1645/GE-2571.1 |
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Universidade Federal da Bahia (UFBA) |
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UFBA |
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