The polymorphism in MUC1 gene in Nelore cattle

Detalhes bibliográficos
Autor(a) principal: Souza, F. R. P. de
Data de Publicação: 2007
Outros Autores: Dentillo, D. B., Meola, J., Biase, F. H., Andréa, M. V., Vozzi, P. A., Lôbo, Raysildo Barbosa, Martelli, L. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/15060
Resumo: Texto completo: acesso restrito. p. 42–46
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spelling Souza, F. R. P. deDentillo, D. B.Meola, J.Biase, F. H.Andréa, M. V.Vozzi, P. A.Lôbo, Raysildo BarbosaMartelli, L. R.Souza, F. R. P. deDentillo, D. B.Meola, J.Biase, F. H.Andréa, M. V.Vozzi, P. A.Lôbo, Raysildo BarbosaMartelli, L. R.2014-06-05T17:46:14Z20070931-2668http://repositorio.ufba.br/ri/handle/ri/15060v. 124, n. 1Texto completo: acesso restrito. p. 42–46MUC1 is a transmembrane glycoprotein expressed on the apical surfaces of the uterine epithelial tissue with predicted functions in protection and cell–cell adhesion. These properties are closely related with the repetitive region variable number of tandem repeats (VNTR)] of the extracellullar domain and with the O-glycosylation in their serine and threonine residues. This study describes a polymerase chain reaction (PCR) protocol to analyse MUC1 bovine genetic polymorphism and demonstrates the existence of a VNTR within the sites for O-glycosylation. Oligonucleotide primers based on the Bos taurus mucin (MUC1) gene sequence GenBank AF399757 were used to amplify five VNTR MUC1 alleles from a study group of 56 pure Nelore bovines. The number of repeats varied between 10 and 24, being more prevalent than the alleles with less number of repeats. The DNA sequence analysis revealed two repeats and one of them presented 100% homology with the bovine consensus sequence already reported. The second repeat showed codons that translate to serine and proline amino acids, which are conserved in the MUC1 of several species. This study is the first description of allelic variation and the VNTR structure in the Nelore breed MUC1 gene, and we suggest that this genetic variability can be tested for association with variation in reproductive traits in Nelore cattle.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-03-12T12:45:12Z No. of bitstreams: 1 j.1439-0388.2007.00628.x.pdf: 636308 bytes, checksum: b035dde8ac318d210990dfa87f1e09f8 (MD5)Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-06-05T17:46:14Z (GMT) No. of bitstreams: 1 j.1439-0388.2007.00628.x.pdf: 636308 bytes, checksum: b035dde8ac318d210990dfa87f1e09f8 (MD5)Made available in DSpace on 2014-06-05T17:46:14Z (GMT). No. of bitstreams: 1 j.1439-0388.2007.00628.x.pdf: 636308 bytes, checksum: b035dde8ac318d210990dfa87f1e09f8 (MD5) Previous issue date: 2007http://dx.doi.org/10.1111/j.1439-0388.2007.00628.xreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACattleMolecular markerPCRVNTRPolymerase Chain ReactionMinisatellite RepeatsGlycosylationGlycosylationOligonucleotide probesOligonucleotidesThe polymorphism in MUC1 gene in Nelore cattleJournal of Animal Breeding and Geneticsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALj.1439-0388.2007.00628.x.pdfj.1439-0388.2007.00628.x.pdfapplication/pdf636308https://repositorio.ufba.br/bitstream/ri/15060/1/j.1439-0388.2007.00628.x.pdfb035dde8ac318d210990dfa87f1e09f8MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15060/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXTj.1439-0388.2007.00628.x.pdf.txtj.1439-0388.2007.00628.x.pdf.txtExtracted texttext/plain19826https://repositorio.ufba.br/bitstream/ri/15060/3/j.1439-0388.2007.00628.x.pdf.txte736b558b608e83b28e259e370c9bc70MD53ri/150602022-07-05 14:03:31.158oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:31Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv The polymorphism in MUC1 gene in Nelore cattle
dc.title.alternative.pt_BR.fl_str_mv Journal of Animal Breeding and Genetics
title The polymorphism in MUC1 gene in Nelore cattle
spellingShingle The polymorphism in MUC1 gene in Nelore cattle
Souza, F. R. P. de
Cattle
Molecular marker
PCR
VNTR
Polymerase Chain Reaction
Minisatellite Repeats
Glycosylation
Glycosylation
Oligonucleotide probes
Oligonucleotides
title_short The polymorphism in MUC1 gene in Nelore cattle
title_full The polymorphism in MUC1 gene in Nelore cattle
title_fullStr The polymorphism in MUC1 gene in Nelore cattle
title_full_unstemmed The polymorphism in MUC1 gene in Nelore cattle
title_sort The polymorphism in MUC1 gene in Nelore cattle
author Souza, F. R. P. de
author_facet Souza, F. R. P. de
Dentillo, D. B.
Meola, J.
Biase, F. H.
Andréa, M. V.
Vozzi, P. A.
Lôbo, Raysildo Barbosa
Martelli, L. R.
author_role author
author2 Dentillo, D. B.
Meola, J.
Biase, F. H.
Andréa, M. V.
Vozzi, P. A.
Lôbo, Raysildo Barbosa
Martelli, L. R.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, F. R. P. de
Dentillo, D. B.
Meola, J.
Biase, F. H.
Andréa, M. V.
Vozzi, P. A.
Lôbo, Raysildo Barbosa
Martelli, L. R.
Souza, F. R. P. de
Dentillo, D. B.
Meola, J.
Biase, F. H.
Andréa, M. V.
Vozzi, P. A.
Lôbo, Raysildo Barbosa
Martelli, L. R.
dc.subject.por.fl_str_mv Cattle
Molecular marker
PCR
VNTR
Polymerase Chain Reaction
Minisatellite Repeats
Glycosylation
Glycosylation
Oligonucleotide probes
Oligonucleotides
topic Cattle
Molecular marker
PCR
VNTR
Polymerase Chain Reaction
Minisatellite Repeats
Glycosylation
Glycosylation
Oligonucleotide probes
Oligonucleotides
description Texto completo: acesso restrito. p. 42–46
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2014-06-05T17:46:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/15060
dc.identifier.issn.none.fl_str_mv 0931-2668
dc.identifier.number.pt_BR.fl_str_mv v. 124, n. 1
identifier_str_mv 0931-2668
v. 124, n. 1
url http://repositorio.ufba.br/ri/handle/ri/15060
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1111/j.1439-0388.2007.00628.x
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