Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells

Detalhes bibliográficos
Autor(a) principal: Souza, Cleide dos Santos
Data de Publicação: 2011
Outros Autores: Chicaybam, Leonardo, Bonamino, Martin Hernán, Bahia, Marcus Vinicius, Costa, Silvia Lima, Borges, Helena L., Rehen, Stevens K.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14750
Resumo: Texto completo: acesso restrito. p. 1711-1720
id UFBA-2_daf32b01176a18e2404d074426292f59
oai_identifier_str oai:repositorio.ufba.br:ri/14750
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Souza, Cleide dos SantosChicaybam, LeonardoBonamino, Martin HernánBahia, Marcus ViniciusCosta, Silvia LimaBorges, Helena L.Rehen, Stevens K.Souza, Cleide dos SantosChicaybam, LeonardoBonamino, Martin HernánBahia, Marcus ViniciusCosta, Silvia LimaBorges, Helena L.Rehen, Stevens K.2014-03-13T18:24:48Z20111547-3287http://repositorio.ufba.br/ri/handle/ri/14750v. 20, n. 10Texto completo: acesso restrito. p. 1711-1720Flavonoids have key functions in the regulation of multiple cellular processes; however, their effects have been poorly examined in pluripotent stem cells. Here, we tested the hypothesis that neurogenesis induced by all-trans retinoic acid (RA) is enhanced by agathisflavone (FAB, Caesalpinia pyramidalis Tull). Mouse embryonic stem (mES) cells and induced pluripotent stem (miPS) cells growing as embryoid bodies (EBs) for 4 days were treated with FAB (60 μM) and/or RA (2 μM) for additional 4 days. FAB did not interfere with the EB mitotic rate of mES cells, as evidenced by similar percentages of mitotic figures labeled by phospho-histone H3 in control (3.4%±0.4%) and FAB-treated groups (3.5%±1.1%). Nevertheless, the biflavonoid reduced cell death in both control and RA-treated EBs from mES cells by almost 2-fold compared with untreated EBs. FAB was unable, by itself, to induce neuronal differentiation in EBs after 4 days of treatment. On the other hand, FAB enhanced neuronal differentiation induced by RA in both EBs of mES and miPS. FAB increased the percentage of nestin-labeled cells by 2.7-fold (mES) and 2.4 (miPS) and β-tubulin III–positive cells by 2-fold (mES) and 2.7 (miPS) in comparison to RA-treated EBs only. FAB increased the expression of RA receptors α and β in mES EBs, suggesting that the availability of RA receptors is limiting RA-induced neurogenesis in pluripotent stem cells. This is the first report to describe that naturally occurring biflavonoids regulate apoptosis and neuronal differentiation in pluripotent stem cells.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-03-13T17:03:21Z No. of bitstreams: 1 Cleide S. Souza.pdf: 801326 bytes, checksum: c5b0023557e1956e9368e5601b957064 (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-03-13T18:24:47Z (GMT) No. of bitstreams: 1 Cleide S. Souza.pdf: 801326 bytes, checksum: c5b0023557e1956e9368e5601b957064 (MD5)Made available in DSpace on 2014-03-13T18:24:48Z (GMT). No. of bitstreams: 1 Cleide S. Souza.pdf: 801326 bytes, checksum: c5b0023557e1956e9368e5601b957064 (MD5) Previous issue date: 2011http://dx.doi.org/ 10.1089/scd.2010.0446reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAAgathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem CellsStem Cells and Developmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALCleide S. Souza.pdfCleide S. Souza.pdfapplication/pdf801326https://repositorio.ufba.br/bitstream/ri/14750/1/Cleide%20S.%20Souza.pdfc5b0023557e1956e9368e5601b957064MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14750/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXTCleide S. Souza.pdf.txtCleide S. Souza.pdf.txtExtracted texttext/plain41202https://repositorio.ufba.br/bitstream/ri/14750/3/Cleide%20S.%20Souza.pdf.txt9f0fa8b1e74fece87de42c0eccaa3e9aMD53ri/147502022-07-05 14:03:12.35oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:12Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells
dc.title.alternative.pt_BR.fl_str_mv Stem Cells and Development
title Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells
spellingShingle Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells
Souza, Cleide dos Santos
title_short Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells
title_full Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells
title_fullStr Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells
title_full_unstemmed Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells
title_sort Agathisflavone Enhances Retinoic Acid-Induced Neurogenesis and Its Receptors α and β in Pluripotent Stem Cells
author Souza, Cleide dos Santos
author_facet Souza, Cleide dos Santos
Chicaybam, Leonardo
Bonamino, Martin Hernán
Bahia, Marcus Vinicius
Costa, Silvia Lima
Borges, Helena L.
Rehen, Stevens K.
author_role author
author2 Chicaybam, Leonardo
Bonamino, Martin Hernán
Bahia, Marcus Vinicius
Costa, Silvia Lima
Borges, Helena L.
Rehen, Stevens K.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Cleide dos Santos
Chicaybam, Leonardo
Bonamino, Martin Hernán
Bahia, Marcus Vinicius
Costa, Silvia Lima
Borges, Helena L.
Rehen, Stevens K.
Souza, Cleide dos Santos
Chicaybam, Leonardo
Bonamino, Martin Hernán
Bahia, Marcus Vinicius
Costa, Silvia Lima
Borges, Helena L.
Rehen, Stevens K.
description Texto completo: acesso restrito. p. 1711-1720
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-03-13T18:24:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14750
dc.identifier.issn.none.fl_str_mv 1547-3287
dc.identifier.number.pt_BR.fl_str_mv v. 20, n. 10
identifier_str_mv 1547-3287
v. 20, n. 10
url http://repositorio.ufba.br/ri/handle/ri/14750
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/ 10.1089/scd.2010.0446
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/14750/1/Cleide%20S.%20Souza.pdf
https://repositorio.ufba.br/bitstream/ri/14750/2/license.txt
https://repositorio.ufba.br/bitstream/ri/14750/3/Cleide%20S.%20Souza.pdf.txt
bitstream.checksum.fl_str_mv c5b0023557e1956e9368e5601b957064
0d4b811ef71182510d2015daa7c8a900
9f0fa8b1e74fece87de42c0eccaa3e9a
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459472230678528

Registros relacionados