Amino acids change liver growth factors gene expression in malnourished rats

Detalhes bibliográficos
Autor(a) principal: Jesus, Rosangela Passos de
Data de Publicação: 2010
Outros Autores: Nardi, Letícia de, Rós, Nancy da, Salaorni, Sibeli, Nagai, Maria Aparecida, Brentani, Maria Mitzi, Akamine, Dirce, Waitzberg, Dan L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13479
Resumo: p.382-387
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spelling Jesus, Rosangela Passos deNardi, Letícia deRós, Nancy daSalaorni, SibeliNagai, Maria AparecidaBrentani, Maria MitziAkamine, DirceWaitzberg, Dan L.Jesus, Rosangela Passos deNardi, Letícia deRós, Nancy daSalaorni, SibeliNagai, Maria AparecidaBrentani, Maria MitziAkamine, DirceWaitzberg, Dan L.2013-11-01T11:54:10Z2013-11-01T11:54:10Z20100212-1611http://repositorio.ufba.br/ri/handle/ri/13479v. 25, n. 3p.382-387Background: Glutamine and proline are metabolized in the liver and may collaborate on its regeneration. Parenteral nutrition (PN) containing either glutamine or proline was given to partially hepatectomized rats. The total RNA content and growth factor gene expression in hepatic remnants was measured, to determine the effects of these amino acid supplementation on the expression of growth factors during liver regeneration. Methods: Wistar rats nourished (HN) and malnourished (HM) were hepatectomized and divided in two groups: 20 receiving PN enriched with Alanyl-Glutamine (HN-Gln and HM-Gln) and 20 PN enriched with proline+alanine (HN-Pro and HM-Pro). The control groups comprised 7 nourished (CN) and 7 malnourished (CM) rats that didn’t undergo surgery. Growth factor and thymidine kinase mRNA levels were measured by RT-PCR. Results: In nourished rats, total hepatic RNA levels were lower in the HN-Gln and HN-Pro groups (0.75 and 0.63g/mg tissue, respectively) than in control group (1.67g/mg tissue) (P<0.05). In malnourished rats, total hepatic RNA content was higher in the HM-Pro group than HN-Pro, HM-Gln, and CM (3.18 vs. 0.63, 0.93 and 1.10g/mg, respectively; P<0.05). Hepatocyte growth factor mRNA was more abundant in the HM-Gln group when compared to CM (0.31 vs. 0.23 arbitrary units) and also in HM-Pro in relation to HM-Gln, HN-Pro, and CM (0.46 vs. 0.33 and 0.23, respectively, P<0.05). Conclusions: Proline or glutamine supplementation in malnourished rats improves total RNA content in the remnant hepatic tissue. Amino acids administration increased HGF gene expression after partial hepatectomy in malnourished rats, with a greater effect of proline than glutamine.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-08-29T15:37:21Z No. of bitstreams: 1 Rosangela4292.pdf: 85585 bytes, checksum: 7ade0f7678afba7363783ba8ee4b7aef (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-01T11:54:10Z (GMT) No. of bitstreams: 1 Rosangela4292.pdf: 85585 bytes, checksum: 7ade0f7678afba7363783ba8ee4b7aef (MD5)Made available in DSpace on 2013-11-01T11:54:10Z (GMT). No. of bitstreams: 1 Rosangela4292.pdf: 85585 bytes, checksum: 7ade0f7678afba7363783ba8ee4b7aef (MD5) Previous issue date: 2010http://dx.doi.org/10.3305/nh.2010.25.3.4292reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAAmino acidsParenteral nutritionLiver rege- nerationProlineGlutamineAmino acids change liver growth factors gene expression in malnourished ratsNutrición Hospitalariainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALRosangela4292.pdfRosangela4292.pdfapplication/pdf85585https://repositorio.ufba.br/bitstream/ri/13479/1/Rosangela4292.pdf7ade0f7678afba7363783ba8ee4b7aefMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/13479/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTRosangela4292.pdf.txtRosangela4292.pdf.txtExtracted texttext/plain28416https://repositorio.ufba.br/bitstream/ri/13479/3/Rosangela4292.pdf.txt8c7e03907ecbaaefdfd23dcf4dc76b5cMD53ri/134792022-08-08 13:18:19.026oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:18:19Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Amino acids change liver growth factors gene expression in malnourished rats
dc.title.alternative.pt_BR.fl_str_mv Nutrición Hospitalaria
title Amino acids change liver growth factors gene expression in malnourished rats
spellingShingle Amino acids change liver growth factors gene expression in malnourished rats
Jesus, Rosangela Passos de
Amino acids
Parenteral nutrition
Liver rege- neration
Proline
Glutamine
title_short Amino acids change liver growth factors gene expression in malnourished rats
title_full Amino acids change liver growth factors gene expression in malnourished rats
title_fullStr Amino acids change liver growth factors gene expression in malnourished rats
title_full_unstemmed Amino acids change liver growth factors gene expression in malnourished rats
title_sort Amino acids change liver growth factors gene expression in malnourished rats
author Jesus, Rosangela Passos de
author_facet Jesus, Rosangela Passos de
Nardi, Letícia de
Rós, Nancy da
Salaorni, Sibeli
Nagai, Maria Aparecida
Brentani, Maria Mitzi
Akamine, Dirce
Waitzberg, Dan L.
author_role author
author2 Nardi, Letícia de
Rós, Nancy da
Salaorni, Sibeli
Nagai, Maria Aparecida
Brentani, Maria Mitzi
Akamine, Dirce
Waitzberg, Dan L.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jesus, Rosangela Passos de
Nardi, Letícia de
Rós, Nancy da
Salaorni, Sibeli
Nagai, Maria Aparecida
Brentani, Maria Mitzi
Akamine, Dirce
Waitzberg, Dan L.
Jesus, Rosangela Passos de
Nardi, Letícia de
Rós, Nancy da
Salaorni, Sibeli
Nagai, Maria Aparecida
Brentani, Maria Mitzi
Akamine, Dirce
Waitzberg, Dan L.
dc.subject.por.fl_str_mv Amino acids
Parenteral nutrition
Liver rege- neration
Proline
Glutamine
topic Amino acids
Parenteral nutrition
Liver rege- neration
Proline
Glutamine
description p.382-387
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2013-11-01T11:54:10Z
dc.date.available.fl_str_mv 2013-11-01T11:54:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13479
dc.identifier.issn.none.fl_str_mv 0212-1611
dc.identifier.number.pt_BR.fl_str_mv v. 25, n. 3
identifier_str_mv 0212-1611
v. 25, n. 3
url http://repositorio.ufba.br/ri/handle/ri/13479
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.3305/nh.2010.25.3.4292
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
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