High degree of conservancy among secreted salivary gland proteins from two geographically distant Phlebotomus duboscqi sandflies populations (Mali and Kenya)

• Autor(a) principal: Kato, Hirotomo
• Data de Publicação: 2006
• Outros Autores: Anderson, Jennifer M., Kamhawi, Shaden, Oliveira, Fabiano, Lawyer, Phillip G., Pham, Van My, Sangare, Constance Souko, Samake, Sibiry, Sissoko, Ibrahim, Garfield, Mark, Sigutova, Lucie, Volf, Petr, Doumbia, Seydou, Valenzuela, Jesus G.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFBA
• Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6493

Background: Salivary proteins from sandflies are potential targets for exploitation as vaccines to control Leishmania infection; in this work we tested the hypothesis that salivary proteins from geographically distant Phlebotomus duboscqi sandfly populations are highly divergent due to the pressure exerted by the host immune response. Salivary gland cDNA libraries were prepared from wild-caught P. duboscqi from Mali and recently colonised flies of the same species from Kenya. Results: Transcriptome and proteome analysis resulted in the identification of the most abundant salivary gland-secreted proteins. Orthologues of these salivary proteins were identified by phylogenetic tree analysis. Moreover, comparative analysis between the orthologues of these two different populations resulted in a high level of protein identity, including the predicted MHC class II T-cell epitopes from all these salivary proteins. Conclusion: These data refute the hypothesis that salivary proteins from geographically distinct populations of the same Phlebotomus sandfly species are highly divergent. They also suggest the potential for using the same species-specific components in a potential vector saliva-based vaccine.