Warifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/12166 |
Resumo: | Texto completo. Acesso restrito. p. 37-44 |
| id |
UFBA-2_fe7e28f9e813672a1c1b762bf031c831 |
|---|---|
| oai_identifier_str |
oai:repositorio.ufba.br:ri/12166 |
| network_acronym_str |
UFBA-2 |
| network_name_str |
Repositório Institucional da UFBA |
| repository_id_str |
1932 |
| spelling |
Assis, Ápio Cláudio de LimaGonçalves, Islania Giselia AlbuquerqueLima, Renata P. C.Almeida, Mônica M.Marinho, Alexsandro FernandesBarbosa Filho, José MariaCruz, Jader dos SantosVasconcelos, Darizy Flavia Silva Amorim deMedeiros, Isac Almeida deAssis, Ápio Cláudio de LimaGonçalves, Islania Giselia AlbuquerqueLima, Renata P. C.Almeida, Mônica M.Marinho, Alexsandro FernandesBarbosa Filho, José MariaCruz, Jader dos SantosVasconcelos, Darizy Flavia Silva Amorim deMedeiros, Isac Almeida de2013-07-11T13:29:29Z2013-07-11T13:29:29Z20130305-1870http://www.repositorio.ufba.br/ri/handle/ri/12166v. 40, n. 1Texto completo. Acesso restrito. p. 37-44The present study used functional and electrophysiological approaches to investigate the mechanisms by which warifteine, a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis Eichl., causes vasorelaxation of the rat thoracic aorta. 2. Warifteine (1 pmol/L–10 lmol/L) induced concentration- dependent relaxation (pD2 = 9.40 ± 0.06; n = 5) of endothelium-intact aortic rings precontracted with noradrenaline (10–100 lmol/L). The relaxation effects were not attenuated by removal of the endothelium. Warifteine also induced the relaxation of prostaglandin F2a (1–10 mmol/L)-precontracted rings (pD2 = 9.2 ± 0.2; n = 8). In contrast, the relaxant activity of warifteine was nearly abolished in high K+ (80 mmol/L)-precontracted aortic rings. In preparations incubated with 20 mmol/L KCl or with the K+ channel blockers tetraethylammonium (1, 3 and 5 mmol/L), iberiotoxin (20 nmol/L), 4-aminopyridine (1 mmol/L) or glibenclamide (10 lmol/L), the vasorelaxant activity of warifteine was markedly reduced. However, BaCl2 (1 mmol/L) had no effect on the relaxant effects of warifteine. 3. In vascular myocytes, warifteine (100 nmol/L) significantly increased whole-cell K+ currents (at 70 mV). Under nominally Ca2+-free conditions, warifteine did not reduce extracellular Ca2+-induced contractions in rings precontracted with high K+ or noradrenaline (100 lmol/L). 4. Together, the results of the present study indicate that warifteine induces potent concentration-dependent relaxation in the rat aorta via an endothelium-independent mechanism that involves the activation of K+ channels.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-07-08T15:11:55Z No. of bitstreams: 1 99999999999999999.pdf: 419451 bytes, checksum: bacc5f7b601e22fce4b4083185ff6439 (MD5)Approved for entry into archive by Rodrigo Meirelles(rodrigomei@ufba.br) on 2013-07-11T13:29:29Z (GMT) No. of bitstreams: 1 99999999999999999.pdf: 419451 bytes, checksum: bacc5f7b601e22fce4b4083185ff6439 (MD5)Made available in DSpace on 2013-07-11T13:29:29Z (GMT). No. of bitstreams: 1 99999999999999999.pdf: 419451 bytes, checksum: bacc5f7b601e22fce4b4083185ff6439 (MD5) Previous issue date: 2013SalvadorClinical and Experimental Pharmacology and Physiology10.1111/1440-1681.12029reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAbisbenzylisoquinoline alkaloidpotassium channelsrat aortavascular smooth muscle cellsvasodilatationwarifteineWarifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytesClinical and Experimental Pharmacology and Physiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL99999999999999999.pdf99999999999999999.pdfapplication/pdf419451https://repositorio.ufba.br/bitstream/ri/12166/1/99999999999999999.pdfbacc5f7b601e22fce4b4083185ff6439MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12166/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT99999999999999999.pdf.txt99999999999999999.pdf.txtExtracted texttext/plain42696https://repositorio.ufba.br/bitstream/ri/12166/3/99999999999999999.pdf.txtd0a8e0f559d21d72472462fa484c1807MD53ri/121662022-08-08 13:17:34.135oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:34Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Warifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytes |
| dc.title.alternative.pt_BR.fl_str_mv |
Clinical and Experimental Pharmacology and Physiology |
| title |
Warifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytes |
| spellingShingle |
Warifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytes Assis, Ápio Cláudio de Lima bisbenzylisoquinoline alkaloid potassium channels rat aorta vascular smooth muscle cells vasodilatation warifteine |
| title_short |
Warifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytes |
| title_full |
Warifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytes |
| title_fullStr |
Warifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytes |
| title_full_unstemmed |
Warifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytes |
| title_sort |
Warifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytes |
| author |
Assis, Ápio Cláudio de Lima |
| author_facet |
Assis, Ápio Cláudio de Lima Gonçalves, Islania Giselia Albuquerque Lima, Renata P. C. Almeida, Mônica M. Marinho, Alexsandro Fernandes Barbosa Filho, José Maria Cruz, Jader dos Santos Vasconcelos, Darizy Flavia Silva Amorim de Medeiros, Isac Almeida de |
| author_role |
author |
| author2 |
Gonçalves, Islania Giselia Albuquerque Lima, Renata P. C. Almeida, Mônica M. Marinho, Alexsandro Fernandes Barbosa Filho, José Maria Cruz, Jader dos Santos Vasconcelos, Darizy Flavia Silva Amorim de Medeiros, Isac Almeida de |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Assis, Ápio Cláudio de Lima Gonçalves, Islania Giselia Albuquerque Lima, Renata P. C. Almeida, Mônica M. Marinho, Alexsandro Fernandes Barbosa Filho, José Maria Cruz, Jader dos Santos Vasconcelos, Darizy Flavia Silva Amorim de Medeiros, Isac Almeida de Assis, Ápio Cláudio de Lima Gonçalves, Islania Giselia Albuquerque Lima, Renata P. C. Almeida, Mônica M. Marinho, Alexsandro Fernandes Barbosa Filho, José Maria Cruz, Jader dos Santos Vasconcelos, Darizy Flavia Silva Amorim de Medeiros, Isac Almeida de |
| dc.subject.por.fl_str_mv |
bisbenzylisoquinoline alkaloid potassium channels rat aorta vascular smooth muscle cells vasodilatation warifteine |
| topic |
bisbenzylisoquinoline alkaloid potassium channels rat aorta vascular smooth muscle cells vasodilatation warifteine |
| description |
Texto completo. Acesso restrito. p. 37-44 |
| publishDate |
2013 |
| dc.date.accessioned.fl_str_mv |
2013-07-11T13:29:29Z |
| dc.date.available.fl_str_mv |
2013-07-11T13:29:29Z |
| dc.date.issued.fl_str_mv |
2013 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/12166 |
| dc.identifier.issn.none.fl_str_mv |
0305-1870 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 40, n. 1 |
| identifier_str_mv |
0305-1870 v. 40, n. 1 |
| url |
http://www.repositorio.ufba.br/ri/handle/ri/12166 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Clinical and Experimental Pharmacology and Physiology |
| publisher.none.fl_str_mv |
Clinical and Experimental Pharmacology and Physiology |
| dc.source.pt_BR.fl_str_mv |
10.1111/1440-1681.12029 |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
| instname_str |
Universidade Federal da Bahia (UFBA) |
| instacron_str |
UFBA |
| institution |
UFBA |
| reponame_str |
Repositório Institucional da UFBA |
| collection |
Repositório Institucional da UFBA |
| bitstream.url.fl_str_mv |
https://repositorio.ufba.br/bitstream/ri/12166/1/99999999999999999.pdf https://repositorio.ufba.br/bitstream/ri/12166/2/license.txt https://repositorio.ufba.br/bitstream/ri/12166/3/99999999999999999.pdf.txt |
| bitstream.checksum.fl_str_mv |
bacc5f7b601e22fce4b4083185ff6439 1b89a9a0548218172d7c829f87a0eab9 d0a8e0f559d21d72472462fa484c1807 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA) |
| repository.mail.fl_str_mv |
|
| _version_ |
1808459451183661056 |