Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Saúde e Produção Animal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402012000200017 |
Resumo: | Nine ruminally cannulated cows fed different energy sources were used to evaluate an avianderived polyclonal antibody preparation against specific ruminal bacteria and monensin on microbial community diversity. The experimental design was three Latin squares 3 x 3 distinguished by the main energy source in the diet [dry-ground corn grain, high moisture corn silage or citrus pulp]. Inside each Latin square, animals received one of the feed additives per period [control, monensin or polyclonal antibody preparation]. Each period lasted 21 days where 20 were used for treatments adaptation and the last one for sampling collection. Microbial diversity was evaluated by protozoa counts and denaturing gradient gel electrophoresis. Polyclonal antibodies plus citrus pulp (CiPu) addition in the diet resulted in an increase of relative counting of Isotricha protozoa that indicates a possible effect on this ruminal ciliate population. In general lines, in the present experiment, it was not possible to assign that there was a pattern in the structures of amplification of Bacteria and Archaea communities of the ruminal content. Oral passive immunization is a technology that arises as an effective alternative for feed additive production. Further research is still necessary to better understand its mechanisms of action. |
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Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sourcesdenaturing gradient gel by electrophoresisfeed additiveionophorespassive immunityprotozoaNine ruminally cannulated cows fed different energy sources were used to evaluate an avianderived polyclonal antibody preparation against specific ruminal bacteria and monensin on microbial community diversity. The experimental design was three Latin squares 3 x 3 distinguished by the main energy source in the diet [dry-ground corn grain, high moisture corn silage or citrus pulp]. Inside each Latin square, animals received one of the feed additives per period [control, monensin or polyclonal antibody preparation]. Each period lasted 21 days where 20 were used for treatments adaptation and the last one for sampling collection. Microbial diversity was evaluated by protozoa counts and denaturing gradient gel electrophoresis. Polyclonal antibodies plus citrus pulp (CiPu) addition in the diet resulted in an increase of relative counting of Isotricha protozoa that indicates a possible effect on this ruminal ciliate population. In general lines, in the present experiment, it was not possible to assign that there was a pattern in the structures of amplification of Bacteria and Archaea communities of the ruminal content. Oral passive immunization is a technology that arises as an effective alternative for feed additive production. Further research is still necessary to better understand its mechanisms of action.UFBA - Universidade Federal da Bahia2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402012000200017Revista Brasileira de Saúde e Produção Animal v.13 n.2 2012reponame:Revista Brasileira de Saúde e Produção Animalinstname:Universidade Federal da Bahia (UFBA)instacron:UFBA10.1590/S1519-99402012000200017info:eu-repo/semantics/openAccessOtero,Walter GuimarãesMarino,Carolina TobiasBarreto,Cristine ChavesPellizari,Vivian HelenaMillen,Danilo DominguesPacheco,Rodrigo Dias LauritanoFerreira,Fernanda AltieriDicostanzo,AlfredoArrigoni,Mário de BeniRodrigues,Paulo Henrique Mazzaeng2014-09-29T00:00:00Zoai:scielo:S1519-99402012000200017Revistahttp://www.rbspa.ufba.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||rbspa@ufba.br1519-99401519-9940opendoar:2014-09-29T00:00Revista Brasileira de Saúde e Produção Animal - Universidade Federal da Bahia (UFBA)false |
dc.title.none.fl_str_mv |
Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources |
title |
Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources |
spellingShingle |
Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources Otero,Walter Guimarães denaturing gradient gel by electrophoresis feed additive ionophores passive immunity protozoa |
title_short |
Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources |
title_full |
Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources |
title_fullStr |
Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources |
title_full_unstemmed |
Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources |
title_sort |
Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources |
author |
Otero,Walter Guimarães |
author_facet |
Otero,Walter Guimarães Marino,Carolina Tobias Barreto,Cristine Chaves Pellizari,Vivian Helena Millen,Danilo Domingues Pacheco,Rodrigo Dias Lauritano Ferreira,Fernanda Altieri Dicostanzo,Alfredo Arrigoni,Mário de Beni Rodrigues,Paulo Henrique Mazza |
author_role |
author |
author2 |
Marino,Carolina Tobias Barreto,Cristine Chaves Pellizari,Vivian Helena Millen,Danilo Domingues Pacheco,Rodrigo Dias Lauritano Ferreira,Fernanda Altieri Dicostanzo,Alfredo Arrigoni,Mário de Beni Rodrigues,Paulo Henrique Mazza |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Otero,Walter Guimarães Marino,Carolina Tobias Barreto,Cristine Chaves Pellizari,Vivian Helena Millen,Danilo Domingues Pacheco,Rodrigo Dias Lauritano Ferreira,Fernanda Altieri Dicostanzo,Alfredo Arrigoni,Mário de Beni Rodrigues,Paulo Henrique Mazza |
dc.subject.por.fl_str_mv |
denaturing gradient gel by electrophoresis feed additive ionophores passive immunity protozoa |
topic |
denaturing gradient gel by electrophoresis feed additive ionophores passive immunity protozoa |
description |
Nine ruminally cannulated cows fed different energy sources were used to evaluate an avianderived polyclonal antibody preparation against specific ruminal bacteria and monensin on microbial community diversity. The experimental design was three Latin squares 3 x 3 distinguished by the main energy source in the diet [dry-ground corn grain, high moisture corn silage or citrus pulp]. Inside each Latin square, animals received one of the feed additives per period [control, monensin or polyclonal antibody preparation]. Each period lasted 21 days where 20 were used for treatments adaptation and the last one for sampling collection. Microbial diversity was evaluated by protozoa counts and denaturing gradient gel electrophoresis. Polyclonal antibodies plus citrus pulp (CiPu) addition in the diet resulted in an increase of relative counting of Isotricha protozoa that indicates a possible effect on this ruminal ciliate population. In general lines, in the present experiment, it was not possible to assign that there was a pattern in the structures of amplification of Bacteria and Archaea communities of the ruminal content. Oral passive immunization is a technology that arises as an effective alternative for feed additive production. Further research is still necessary to better understand its mechanisms of action. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402012000200017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402012000200017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1519-99402012000200017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
UFBA - Universidade Federal da Bahia |
publisher.none.fl_str_mv |
UFBA - Universidade Federal da Bahia |
dc.source.none.fl_str_mv |
Revista Brasileira de Saúde e Produção Animal v.13 n.2 2012 reponame:Revista Brasileira de Saúde e Produção Animal instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
instname_str |
Universidade Federal da Bahia (UFBA) |
instacron_str |
UFBA |
institution |
UFBA |
reponame_str |
Revista Brasileira de Saúde e Produção Animal |
collection |
Revista Brasileira de Saúde e Produção Animal |
repository.name.fl_str_mv |
Revista Brasileira de Saúde e Produção Animal - Universidade Federal da Bahia (UFBA) |
repository.mail.fl_str_mv |
||rbspa@ufba.br |
_version_ |
1750297506120466432 |