Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis

Detalhes bibliográficos
Autor(a) principal: Victório,Cristiane Pimentel
Data de Publicação: 2012
Outros Autores: Lage,Celso Luiz Salgueiro, Sato,Alice
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista ciência agronômica (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000300017
Resumo: The high demand for plant material from Calendula officinalis in the production of herbal medicines and cosmetics, turns the technique of plant-tissue culture into one of the alternatives for the improvement of crops over a short period of time. A protocol for tissue culture was developed from segments of seedlings of C. officinalis, in order to improve the proliferation of shoots and roots. We used a Murashige and Skoog (MS½N) medium, reduced to half the concentrations of NH4NO3 and KNO3 to verify the effect of different types of explants (basal, intermediate, and apical), a medium containing beach sand as support instead of agar, and the effect of auxins and cytokinins (TDZ tidiazuron; BAP, 6-benzylaminopurine, IAA, indol-3-acetic acid, IBA, indol-3-butyric acid, NAA, naphthalene-acetic acid) on plant development in vitro. The results showed pronounced rooting from the apical explants, as well as a greater elongation of shoots and number of leaves. The solid medium was more suitable for the C. officinalis cultures. Shoot proliferation was dependent on cytokinins with better results from the influence of TDZ or BAP compared to the other treatments. Plants regenerated from the medium containing TDZ displayed a glazed appearance and morphogenetic deformations. The highest rate for rooting (80%) was obtained using IAA 0.1mgL-1. Through in-vitro propagation, healthy C. officinalis plants were obtained with roots which can acclimatise, allowing the continuous supplement of raw material.
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spelling Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalisMedicinal plantShoots (Plants)Plant-tissue culturePlant-growth regulatorsThe high demand for plant material from Calendula officinalis in the production of herbal medicines and cosmetics, turns the technique of plant-tissue culture into one of the alternatives for the improvement of crops over a short period of time. A protocol for tissue culture was developed from segments of seedlings of C. officinalis, in order to improve the proliferation of shoots and roots. We used a Murashige and Skoog (MS½N) medium, reduced to half the concentrations of NH4NO3 and KNO3 to verify the effect of different types of explants (basal, intermediate, and apical), a medium containing beach sand as support instead of agar, and the effect of auxins and cytokinins (TDZ tidiazuron; BAP, 6-benzylaminopurine, IAA, indol-3-acetic acid, IBA, indol-3-butyric acid, NAA, naphthalene-acetic acid) on plant development in vitro. The results showed pronounced rooting from the apical explants, as well as a greater elongation of shoots and number of leaves. The solid medium was more suitable for the C. officinalis cultures. Shoot proliferation was dependent on cytokinins with better results from the influence of TDZ or BAP compared to the other treatments. Plants regenerated from the medium containing TDZ displayed a glazed appearance and morphogenetic deformations. The highest rate for rooting (80%) was obtained using IAA 0.1mgL-1. Through in-vitro propagation, healthy C. officinalis plants were obtained with roots which can acclimatise, allowing the continuous supplement of raw material.Universidade Federal do Ceará2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000300017Revista Ciência Agronômica v.43 n.3 2012reponame:Revista ciência agronômica (Online)instname:Universidade Federal do Ceará (UFC)instacron:UFC10.1590/S1806-66902012000300017info:eu-repo/semantics/openAccessVictório,Cristiane PimentelLage,Celso Luiz SalgueiroSato,Aliceeng2012-04-12T00:00:00Zoai:scielo:S1806-66902012000300017Revistahttp://www.ccarevista.ufc.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||alekdutra@ufc.br|| ccarev@ufc.br1806-66900045-6888opendoar:2012-04-12T00:00Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis
title Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis
spellingShingle Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis
Victório,Cristiane Pimentel
Medicinal plant
Shoots (Plants)
Plant-tissue culture
Plant-growth regulators
title_short Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis
title_full Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis
title_fullStr Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis
title_full_unstemmed Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis
title_sort Tissue culture techniques in the proliferation of shoots and roots of Calendula officinalis
author Victório,Cristiane Pimentel
author_facet Victório,Cristiane Pimentel
Lage,Celso Luiz Salgueiro
Sato,Alice
author_role author
author2 Lage,Celso Luiz Salgueiro
Sato,Alice
author2_role author
author
dc.contributor.author.fl_str_mv Victório,Cristiane Pimentel
Lage,Celso Luiz Salgueiro
Sato,Alice
dc.subject.por.fl_str_mv Medicinal plant
Shoots (Plants)
Plant-tissue culture
Plant-growth regulators
topic Medicinal plant
Shoots (Plants)
Plant-tissue culture
Plant-growth regulators
description The high demand for plant material from Calendula officinalis in the production of herbal medicines and cosmetics, turns the technique of plant-tissue culture into one of the alternatives for the improvement of crops over a short period of time. A protocol for tissue culture was developed from segments of seedlings of C. officinalis, in order to improve the proliferation of shoots and roots. We used a Murashige and Skoog (MS½N) medium, reduced to half the concentrations of NH4NO3 and KNO3 to verify the effect of different types of explants (basal, intermediate, and apical), a medium containing beach sand as support instead of agar, and the effect of auxins and cytokinins (TDZ tidiazuron; BAP, 6-benzylaminopurine, IAA, indol-3-acetic acid, IBA, indol-3-butyric acid, NAA, naphthalene-acetic acid) on plant development in vitro. The results showed pronounced rooting from the apical explants, as well as a greater elongation of shoots and number of leaves. The solid medium was more suitable for the C. officinalis cultures. Shoot proliferation was dependent on cytokinins with better results from the influence of TDZ or BAP compared to the other treatments. Plants regenerated from the medium containing TDZ displayed a glazed appearance and morphogenetic deformations. The highest rate for rooting (80%) was obtained using IAA 0.1mgL-1. Through in-vitro propagation, healthy C. officinalis plants were obtained with roots which can acclimatise, allowing the continuous supplement of raw material.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000300017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000300017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1806-66902012000300017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal do Ceará
publisher.none.fl_str_mv Universidade Federal do Ceará
dc.source.none.fl_str_mv Revista Ciência Agronômica v.43 n.3 2012
reponame:Revista ciência agronômica (Online)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Revista ciência agronômica (Online)
collection Revista ciência agronômica (Online)
repository.name.fl_str_mv Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv ||alekdutra@ufc.br|| ccarev@ufc.br
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