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Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño

Detalhes bibliográficos
Autor(a) principal: Santos,Mauricio Reginaldo Alves dos
Data de Publicação: 2017
Outros Autores: Souza,Carolina Augusto de, Paz,Eloísa Santana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista ciência agronômica (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000300523
Resumo: ABSTRACT The genus Capsicum belongs to the Solanaceae botanical family and is notable for the production of secondary metabolites of medicinal and economical importance. In vitro methods have been successfully utilized for the large scale production of plant secondary metabolites. The objective of this study was to establish a protocol for callus induction from leaves, nodal and internodal segments of the cultivar C. annuum var. annuum cv. Iberaba Jalapeño, and to determine the growth pattern of the calluses, aiming at the identification of the deceleration phase, when the callus cells must be subcultured for the establishment of cell suspensions and the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combination. Percentage of callus induction and the explant area covered by callus cells (ACCC) were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The highest callus induction percentage and ACCC were observed with the combination of 2.22 µM BA + 18.10 µM 2,4-D for leaf and internodal explants and 2.22 µM BA + 9.05 µM 2,4-D for nodal explants. The calluses produced were friable and whitish, and their growth pattern followed a sigmoid shape. The deceleration phase started on the 31st day of cultivation for leaf explants, on the 26th for internodal explants, and on the 29th day for nodal explants.
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spelling Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba JalapeñoCallogenesisGrowth curveSecondary metabolitesABSTRACT The genus Capsicum belongs to the Solanaceae botanical family and is notable for the production of secondary metabolites of medicinal and economical importance. In vitro methods have been successfully utilized for the large scale production of plant secondary metabolites. The objective of this study was to establish a protocol for callus induction from leaves, nodal and internodal segments of the cultivar C. annuum var. annuum cv. Iberaba Jalapeño, and to determine the growth pattern of the calluses, aiming at the identification of the deceleration phase, when the callus cells must be subcultured for the establishment of cell suspensions and the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combination. Percentage of callus induction and the explant area covered by callus cells (ACCC) were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The highest callus induction percentage and ACCC were observed with the combination of 2.22 µM BA + 18.10 µM 2,4-D for leaf and internodal explants and 2.22 µM BA + 9.05 µM 2,4-D for nodal explants. The calluses produced were friable and whitish, and their growth pattern followed a sigmoid shape. The deceleration phase started on the 31st day of cultivation for leaf explants, on the 26th for internodal explants, and on the 29th day for nodal explants.Universidade Federal do Ceará2017-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000300523Revista Ciência Agronômica v.48 n.3 2017reponame:Revista ciência agronômica (Online)instname:Universidade Federal do Ceará (UFC)instacron:UFC10.5935/1806-6690.20170061info:eu-repo/semantics/openAccessSantos,Mauricio Reginaldo Alves dosSouza,Carolina Augusto dePaz,Eloísa Santanaeng2017-05-22T00:00:00Zoai:scielo:S1806-66902017000300523Revistahttp://www.ccarevista.ufc.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||alekdutra@ufc.br|| ccarev@ufc.br1806-66900045-6888opendoar:2017-05-22T00:00Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño
title Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño
spellingShingle Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño
Santos,Mauricio Reginaldo Alves dos
Callogenesis
Growth curve
Secondary metabolites
title_short Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño
title_full Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño
title_fullStr Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño
title_full_unstemmed Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño
title_sort Growth pattern of friable calluses from leaves of Capsicum annuum var. annuum cv. Iberaba Jalapeño
author Santos,Mauricio Reginaldo Alves dos
author_facet Santos,Mauricio Reginaldo Alves dos
Souza,Carolina Augusto de
Paz,Eloísa Santana
author_role author
author2 Souza,Carolina Augusto de
Paz,Eloísa Santana
author2_role author
author
dc.contributor.author.fl_str_mv Santos,Mauricio Reginaldo Alves dos
Souza,Carolina Augusto de
Paz,Eloísa Santana
dc.subject.por.fl_str_mv Callogenesis
Growth curve
Secondary metabolites
topic Callogenesis
Growth curve
Secondary metabolites
description ABSTRACT The genus Capsicum belongs to the Solanaceae botanical family and is notable for the production of secondary metabolites of medicinal and economical importance. In vitro methods have been successfully utilized for the large scale production of plant secondary metabolites. The objective of this study was to establish a protocol for callus induction from leaves, nodal and internodal segments of the cultivar C. annuum var. annuum cv. Iberaba Jalapeño, and to determine the growth pattern of the calluses, aiming at the identification of the deceleration phase, when the callus cells must be subcultured for the establishment of cell suspensions and the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combination. Percentage of callus induction and the explant area covered by callus cells (ACCC) were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The highest callus induction percentage and ACCC were observed with the combination of 2.22 µM BA + 18.10 µM 2,4-D for leaf and internodal explants and 2.22 µM BA + 9.05 µM 2,4-D for nodal explants. The calluses produced were friable and whitish, and their growth pattern followed a sigmoid shape. The deceleration phase started on the 31st day of cultivation for leaf explants, on the 26th for internodal explants, and on the 29th day for nodal explants.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000300523
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000300523
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/1806-6690.20170061
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal do Ceará
publisher.none.fl_str_mv Universidade Federal do Ceará
dc.source.none.fl_str_mv Revista Ciência Agronômica v.48 n.3 2017
reponame:Revista ciência agronômica (Online)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Revista ciência agronômica (Online)
collection Revista ciência agronômica (Online)
repository.name.fl_str_mv Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv ||alekdutra@ufc.br|| ccarev@ufc.br
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