Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista ciência agronômica (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000500001 |
Resumo: | The no-tillage (NT) system of soil management is recognized as more sustainable than conventional tillage (CT), with an important role played by soil microorganisms. The objective of this study was to estimate differences in soil microbial diversity under NT and CT at different soil depths. For that, bacterial (16S rDNA) and fungal (18S rDNA) communities were evaluated by denaturing gradient gel electrophoresis (DGGE) in a 20-year field experiment established on an Oxisol in southern Brazil on which soybean has been grown in the summer and wheat in the winter. Soil samples were collected at the depths of 0-5, 5-10, 10-20 and 20-30 cm, and submitted to DGGE analyses. The results revealed lower similarity (28%) between bacterial communities in the NT and the CT systems at the 0-5 cm layer. The Shannon index (H) confirmed higher bacterial diversity with NT at all depths, when compared to CT. In relation to fungal communities, higher diversity was detected with CT, especially at the 0 - 5-cm depth. The results indicate that fungal communities can be more tolerant to environmental stresses related to soil disturbance than bacteria. More emphasis should be given for understanding processes affecting the diversity of microorganisms in agricultural soils, with particular emphasis on tillage systems. |
id |
UFC-2_bfc4e0509fd2e414283ab2af936f9068 |
---|---|
oai_identifier_str |
oai:scielo:S1806-66902014000500001 |
network_acronym_str |
UFC-2 |
network_name_str |
Revista ciência agronômica (Online) |
repository_id_str |
|
spelling |
Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern BrazilBacterial diversityFungal diversityPCR-DGGESoil tillageThe no-tillage (NT) system of soil management is recognized as more sustainable than conventional tillage (CT), with an important role played by soil microorganisms. The objective of this study was to estimate differences in soil microbial diversity under NT and CT at different soil depths. For that, bacterial (16S rDNA) and fungal (18S rDNA) communities were evaluated by denaturing gradient gel electrophoresis (DGGE) in a 20-year field experiment established on an Oxisol in southern Brazil on which soybean has been grown in the summer and wheat in the winter. Soil samples were collected at the depths of 0-5, 5-10, 10-20 and 20-30 cm, and submitted to DGGE analyses. The results revealed lower similarity (28%) between bacterial communities in the NT and the CT systems at the 0-5 cm layer. The Shannon index (H) confirmed higher bacterial diversity with NT at all depths, when compared to CT. In relation to fungal communities, higher diversity was detected with CT, especially at the 0 - 5-cm depth. The results indicate that fungal communities can be more tolerant to environmental stresses related to soil disturbance than bacteria. More emphasis should be given for understanding processes affecting the diversity of microorganisms in agricultural soils, with particular emphasis on tillage systems.Universidade Federal do Ceará2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000500001Revista Ciência Agronômica v.45 n.5spe 2014reponame:Revista ciência agronômica (Online)instname:Universidade Federal do Ceará (UFC)instacron:UFC10.1590/S1806-66902014000500001info:eu-repo/semantics/openAccessBabujia,Letícia CarlosSilva,Adriana PereiraNogueira,Marco AntonioHungria,Mariangelaeng2014-10-28T00:00:00Zoai:scielo:S1806-66902014000500001Revistahttp://www.ccarevista.ufc.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||alekdutra@ufc.br|| ccarev@ufc.br1806-66900045-6888opendoar:2014-10-28T00:00Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil |
title |
Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil |
spellingShingle |
Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil Babujia,Letícia Carlos Bacterial diversity Fungal diversity PCR-DGGE Soil tillage |
title_short |
Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil |
title_full |
Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil |
title_fullStr |
Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil |
title_full_unstemmed |
Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil |
title_sort |
Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil |
author |
Babujia,Letícia Carlos |
author_facet |
Babujia,Letícia Carlos Silva,Adriana Pereira Nogueira,Marco Antonio Hungria,Mariangela |
author_role |
author |
author2 |
Silva,Adriana Pereira Nogueira,Marco Antonio Hungria,Mariangela |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Babujia,Letícia Carlos Silva,Adriana Pereira Nogueira,Marco Antonio Hungria,Mariangela |
dc.subject.por.fl_str_mv |
Bacterial diversity Fungal diversity PCR-DGGE Soil tillage |
topic |
Bacterial diversity Fungal diversity PCR-DGGE Soil tillage |
description |
The no-tillage (NT) system of soil management is recognized as more sustainable than conventional tillage (CT), with an important role played by soil microorganisms. The objective of this study was to estimate differences in soil microbial diversity under NT and CT at different soil depths. For that, bacterial (16S rDNA) and fungal (18S rDNA) communities were evaluated by denaturing gradient gel electrophoresis (DGGE) in a 20-year field experiment established on an Oxisol in southern Brazil on which soybean has been grown in the summer and wheat in the winter. Soil samples were collected at the depths of 0-5, 5-10, 10-20 and 20-30 cm, and submitted to DGGE analyses. The results revealed lower similarity (28%) between bacterial communities in the NT and the CT systems at the 0-5 cm layer. The Shannon index (H) confirmed higher bacterial diversity with NT at all depths, when compared to CT. In relation to fungal communities, higher diversity was detected with CT, especially at the 0 - 5-cm depth. The results indicate that fungal communities can be more tolerant to environmental stresses related to soil disturbance than bacteria. More emphasis should be given for understanding processes affecting the diversity of microorganisms in agricultural soils, with particular emphasis on tillage systems. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000500001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000500001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1806-66902014000500001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal do Ceará |
publisher.none.fl_str_mv |
Universidade Federal do Ceará |
dc.source.none.fl_str_mv |
Revista Ciência Agronômica v.45 n.5spe 2014 reponame:Revista ciência agronômica (Online) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Revista ciência agronômica (Online) |
collection |
Revista ciência agronômica (Online) |
repository.name.fl_str_mv |
Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
||alekdutra@ufc.br|| ccarev@ufc.br |
_version_ |
1750297487501950976 |