Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated

Bibliographic Details
Main Author: Zambotti, Beatriz Belloni; UNIR/EMBRAPA
Publication Date: 2020
Other Authors: Martines, Mariela Aparecida Claro; Universidade Paulista Júlio de Mesquita Filho (UNESP) – Jaboticabal., Dreibi, Marina Lopes Mechler; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal., Storino, Gabriel Yuri; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal, Ferreira, Marcela Manduca; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal., Oliveira, Luís Guilherme de; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal
Format: Article
Language: por
Source: Revista brasileira de higiene e sanidade animal
Download full: http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/565
Summary: Pigs, in natural conditions, can become infected with the bovine viral diarrhea virus (BVDV), a pestivirus of ruminants that leads to reproductive and economic losses. The final third of pregnancy is a critical period for gilts due to stress and the consequent immunological drop. Thus, the objective was to promote the experimental inoculation of gilts with BVDV-2 and to evaluate the capacity of virus transmission to fetuses in a critical period of stress and immunosuppression. Six pregnant gilts were inoculated between the 105th and 110th day of gestation, the piglets born underwent blood collection (to obtain whole blood and serum) and an umbilical cord fragment at birth prior to colostrum ingestion, and had blood collected weekly up to 21 days of age. Samples from all placentas were also collected. Two gilts composed the control group, and their piglets underwent the same procedures. The blood, umbilical cord and placental fragments samples were submitted to RT-PCR evaluations, while the serum was evaluated by the virus neutralization test. There was no evidence of viral transplacental transmission to fetuses in the evaluated period.  
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spelling Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculatedBVDV, experimental inoculation, piglets, reproductive lossesPigs, in natural conditions, can become infected with the bovine viral diarrhea virus (BVDV), a pestivirus of ruminants that leads to reproductive and economic losses. The final third of pregnancy is a critical period for gilts due to stress and the consequent immunological drop. Thus, the objective was to promote the experimental inoculation of gilts with BVDV-2 and to evaluate the capacity of virus transmission to fetuses in a critical period of stress and immunosuppression. Six pregnant gilts were inoculated between the 105th and 110th day of gestation, the piglets born underwent blood collection (to obtain whole blood and serum) and an umbilical cord fragment at birth prior to colostrum ingestion, and had blood collected weekly up to 21 days of age. Samples from all placentas were also collected. Two gilts composed the control group, and their piglets underwent the same procedures. The blood, umbilical cord and placental fragments samples were submitted to RT-PCR evaluations, while the serum was evaluated by the virus neutralization test. There was no evidence of viral transplacental transmission to fetuses in the evaluated period.  Revista Brasileira de Higiene e Sanidade Animal2020-05-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/56510.5935/rbhsa.v14i2.565Revista Brasileira de Higiene e Sanidade Animal; v. 14, n. 2 (2020); 158-166reponame:Revista brasileira de higiene e sanidade animalinstname:Universidade Federal do Ceará (UFC)instacron:UFCporhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/565/2847Zambotti, Beatriz Belloni; UNIR/EMBRAPAMartines, Mariela Aparecida Claro; Universidade Paulista Júlio de Mesquita Filho (UNESP) – Jaboticabal.Dreibi, Marina Lopes Mechler; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal.Storino, Gabriel Yuri; Universidade Paulista Júlio de Mesquita filho (UNESP) – JaboticabalFerreira, Marcela Manduca; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal.Oliveira, Luís Guilherme de; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabalinfo:eu-repo/semantics/openAccess2021-03-24T14:18:45Zoai:ojs.www.higieneanimal.ufc.br:article/565Revistahttp://www.higieneanimal.ufc.br/PUBhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/oaiwesleylyeverton@yahoo.com.br||ronaldo.sales@ufc.br1981-29651981-2965opendoar:2021-03-24T14:18:45Revista brasileira de higiene e sanidade animal - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated
title Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated
spellingShingle Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated
Zambotti, Beatriz Belloni; UNIR/EMBRAPA
BVDV, experimental inoculation, piglets, reproductive losses
title_short Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated
title_full Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated
title_fullStr Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated
title_full_unstemmed Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated
title_sort Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated
author Zambotti, Beatriz Belloni; UNIR/EMBRAPA
author_facet Zambotti, Beatriz Belloni; UNIR/EMBRAPA
Martines, Mariela Aparecida Claro; Universidade Paulista Júlio de Mesquita Filho (UNESP) – Jaboticabal.
Dreibi, Marina Lopes Mechler; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal.
Storino, Gabriel Yuri; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal
Ferreira, Marcela Manduca; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal.
Oliveira, Luís Guilherme de; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal
author_role author
author2 Martines, Mariela Aparecida Claro; Universidade Paulista Júlio de Mesquita Filho (UNESP) – Jaboticabal.
Dreibi, Marina Lopes Mechler; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal.
Storino, Gabriel Yuri; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal
Ferreira, Marcela Manduca; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal.
Oliveira, Luís Guilherme de; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Zambotti, Beatriz Belloni; UNIR/EMBRAPA
Martines, Mariela Aparecida Claro; Universidade Paulista Júlio de Mesquita Filho (UNESP) – Jaboticabal.
Dreibi, Marina Lopes Mechler; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal.
Storino, Gabriel Yuri; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal
Ferreira, Marcela Manduca; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal.
Oliveira, Luís Guilherme de; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal
dc.subject.por.fl_str_mv BVDV, experimental inoculation, piglets, reproductive losses
topic BVDV, experimental inoculation, piglets, reproductive losses
description Pigs, in natural conditions, can become infected with the bovine viral diarrhea virus (BVDV), a pestivirus of ruminants that leads to reproductive and economic losses. The final third of pregnancy is a critical period for gilts due to stress and the consequent immunological drop. Thus, the objective was to promote the experimental inoculation of gilts with BVDV-2 and to evaluate the capacity of virus transmission to fetuses in a critical period of stress and immunosuppression. Six pregnant gilts were inoculated between the 105th and 110th day of gestation, the piglets born underwent blood collection (to obtain whole blood and serum) and an umbilical cord fragment at birth prior to colostrum ingestion, and had blood collected weekly up to 21 days of age. Samples from all placentas were also collected. Two gilts composed the control group, and their piglets underwent the same procedures. The blood, umbilical cord and placental fragments samples were submitted to RT-PCR evaluations, while the serum was evaluated by the virus neutralization test. There was no evidence of viral transplacental transmission to fetuses in the evaluated period.  
publishDate 2020
dc.date.none.fl_str_mv 2020-05-24
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format article
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dc.identifier.uri.fl_str_mv http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/565
10.5935/rbhsa.v14i2.565
url http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/565
identifier_str_mv 10.5935/rbhsa.v14i2.565
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/565/2847
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Revista Brasileira de Higiene e Sanidade Animal
publisher.none.fl_str_mv Revista Brasileira de Higiene e Sanidade Animal
dc.source.none.fl_str_mv Revista Brasileira de Higiene e Sanidade Animal; v. 14, n. 2 (2020); 158-166
reponame:Revista brasileira de higiene e sanidade animal
instname:Universidade Federal do Ceará (UFC)
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instname_str Universidade Federal do Ceará (UFC)
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collection Revista brasileira de higiene e sanidade animal
repository.name.fl_str_mv Revista brasileira de higiene e sanidade animal - Universidade Federal do Ceará (UFC)
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