Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated
Main Author: | |
---|---|
Publication Date: | 2020 |
Other Authors: | , , , , |
Format: | Article |
Language: | por |
Source: | Revista brasileira de higiene e sanidade animal |
Download full: | http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/565 |
Summary: | Pigs, in natural conditions, can become infected with the bovine viral diarrhea virus (BVDV), a pestivirus of ruminants that leads to reproductive and economic losses. The final third of pregnancy is a critical period for gilts due to stress and the consequent immunological drop. Thus, the objective was to promote the experimental inoculation of gilts with BVDV-2 and to evaluate the capacity of virus transmission to fetuses in a critical period of stress and immunosuppression. Six pregnant gilts were inoculated between the 105th and 110th day of gestation, the piglets born underwent blood collection (to obtain whole blood and serum) and an umbilical cord fragment at birth prior to colostrum ingestion, and had blood collected weekly up to 21 days of age. Samples from all placentas were also collected. Two gilts composed the control group, and their piglets underwent the same procedures. The blood, umbilical cord and placental fragments samples were submitted to RT-PCR evaluations, while the serum was evaluated by the virus neutralization test. There was no evidence of viral transplacental transmission to fetuses in the evaluated period. |
id |
UFC-4_3e7e8535627fe976d8446744b2bdd139 |
---|---|
oai_identifier_str |
oai:ojs.www.higieneanimal.ufc.br:article/565 |
network_acronym_str |
UFC-4 |
network_name_str |
Revista brasileira de higiene e sanidade animal |
repository_id_str |
|
spelling |
Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculatedBVDV, experimental inoculation, piglets, reproductive lossesPigs, in natural conditions, can become infected with the bovine viral diarrhea virus (BVDV), a pestivirus of ruminants that leads to reproductive and economic losses. The final third of pregnancy is a critical period for gilts due to stress and the consequent immunological drop. Thus, the objective was to promote the experimental inoculation of gilts with BVDV-2 and to evaluate the capacity of virus transmission to fetuses in a critical period of stress and immunosuppression. Six pregnant gilts were inoculated between the 105th and 110th day of gestation, the piglets born underwent blood collection (to obtain whole blood and serum) and an umbilical cord fragment at birth prior to colostrum ingestion, and had blood collected weekly up to 21 days of age. Samples from all placentas were also collected. Two gilts composed the control group, and their piglets underwent the same procedures. The blood, umbilical cord and placental fragments samples were submitted to RT-PCR evaluations, while the serum was evaluated by the virus neutralization test. There was no evidence of viral transplacental transmission to fetuses in the evaluated period. Revista Brasileira de Higiene e Sanidade Animal2020-05-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/56510.5935/rbhsa.v14i2.565Revista Brasileira de Higiene e Sanidade Animal; v. 14, n. 2 (2020); 158-166reponame:Revista brasileira de higiene e sanidade animalinstname:Universidade Federal do Ceará (UFC)instacron:UFCporhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/565/2847Zambotti, Beatriz Belloni; UNIR/EMBRAPAMartines, Mariela Aparecida Claro; Universidade Paulista Júlio de Mesquita Filho (UNESP) – Jaboticabal.Dreibi, Marina Lopes Mechler; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal.Storino, Gabriel Yuri; Universidade Paulista Júlio de Mesquita filho (UNESP) – JaboticabalFerreira, Marcela Manduca; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal.Oliveira, Luís Guilherme de; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabalinfo:eu-repo/semantics/openAccess2021-03-24T14:18:45Zoai:ojs.www.higieneanimal.ufc.br:article/565Revistahttp://www.higieneanimal.ufc.br/PUBhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/oaiwesleylyeverton@yahoo.com.br||ronaldo.sales@ufc.br1981-29651981-2965opendoar:2021-03-24T14:18:45Revista brasileira de higiene e sanidade animal - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated |
title |
Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated |
spellingShingle |
Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated Zambotti, Beatriz Belloni; UNIR/EMBRAPA BVDV, experimental inoculation, piglets, reproductive losses |
title_short |
Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated |
title_full |
Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated |
title_fullStr |
Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated |
title_full_unstemmed |
Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated |
title_sort |
Evaluation of the transmission of Bovine Viral Diarrhea Virus by transplacental route in pregnant females experimentally inoculated |
author |
Zambotti, Beatriz Belloni; UNIR/EMBRAPA |
author_facet |
Zambotti, Beatriz Belloni; UNIR/EMBRAPA Martines, Mariela Aparecida Claro; Universidade Paulista Júlio de Mesquita Filho (UNESP) – Jaboticabal. Dreibi, Marina Lopes Mechler; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal. Storino, Gabriel Yuri; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal Ferreira, Marcela Manduca; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal. Oliveira, Luís Guilherme de; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal |
author_role |
author |
author2 |
Martines, Mariela Aparecida Claro; Universidade Paulista Júlio de Mesquita Filho (UNESP) – Jaboticabal. Dreibi, Marina Lopes Mechler; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal. Storino, Gabriel Yuri; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal Ferreira, Marcela Manduca; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal. Oliveira, Luís Guilherme de; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Zambotti, Beatriz Belloni; UNIR/EMBRAPA Martines, Mariela Aparecida Claro; Universidade Paulista Júlio de Mesquita Filho (UNESP) – Jaboticabal. Dreibi, Marina Lopes Mechler; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal. Storino, Gabriel Yuri; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal Ferreira, Marcela Manduca; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal. Oliveira, Luís Guilherme de; Universidade Paulista Júlio de Mesquita filho (UNESP) – Jaboticabal |
dc.subject.por.fl_str_mv |
BVDV, experimental inoculation, piglets, reproductive losses |
topic |
BVDV, experimental inoculation, piglets, reproductive losses |
description |
Pigs, in natural conditions, can become infected with the bovine viral diarrhea virus (BVDV), a pestivirus of ruminants that leads to reproductive and economic losses. The final third of pregnancy is a critical period for gilts due to stress and the consequent immunological drop. Thus, the objective was to promote the experimental inoculation of gilts with BVDV-2 and to evaluate the capacity of virus transmission to fetuses in a critical period of stress and immunosuppression. Six pregnant gilts were inoculated between the 105th and 110th day of gestation, the piglets born underwent blood collection (to obtain whole blood and serum) and an umbilical cord fragment at birth prior to colostrum ingestion, and had blood collected weekly up to 21 days of age. Samples from all placentas were also collected. Two gilts composed the control group, and their piglets underwent the same procedures. The blood, umbilical cord and placental fragments samples were submitted to RT-PCR evaluations, while the serum was evaluated by the virus neutralization test. There was no evidence of viral transplacental transmission to fetuses in the evaluated period. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/565 10.5935/rbhsa.v14i2.565 |
url |
http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/565 |
identifier_str_mv |
10.5935/rbhsa.v14i2.565 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/565/2847 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Revista Brasileira de Higiene e Sanidade Animal |
publisher.none.fl_str_mv |
Revista Brasileira de Higiene e Sanidade Animal |
dc.source.none.fl_str_mv |
Revista Brasileira de Higiene e Sanidade Animal; v. 14, n. 2 (2020); 158-166 reponame:Revista brasileira de higiene e sanidade animal instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Revista brasileira de higiene e sanidade animal |
collection |
Revista brasileira de higiene e sanidade animal |
repository.name.fl_str_mv |
Revista brasileira de higiene e sanidade animal - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
wesleylyeverton@yahoo.com.br||ronaldo.sales@ufc.br |
_version_ |
1814256475127676928 |