The effect of using different energy sources and levels on carcass weight, breast muscle mass, mTORC1 and AKt gene expression in Cobb 500 broiler chicks
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista brasileira de higiene e sanidade animal |
Texto Completo: | http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/687 |
Resumo: | The present study was aimed to evaluate the effect of different energy sources and levels on carcass weight, breast muscle mass mTORC1 and AKt gene expression in Cobb 500 broiler chicks. A total of 600 1-day-old Cobb 500 broiler chicks with an average weight of 39 ± 0.50 g were randomly divided into five treatments. Each treatment was further divided into four replicates. Chicks were fed by basal diet based on corn and energy level by Cobb 500 instruction manual as control group, basal diet with 3% lesser energy than control (T1), basal diet with 6% lesser energy than control (T2), basal diet based on corn and fat level according to Cobb 500 instruction manual (T3), basal diet based on corn and fat with 3% upper energy (T4) for 42 days. To assess carcass characteristics two chicks of each replication were slaughtered and weighted after picking the carcasses. After DNA extraction, the UV spectrophotometric method was performed for determines the quality and quantity and Real-time PCR was performed on the corresponding cDNA synthesized from each sample. Data from this study showed that there were significant differences about carcass and breast muscle weight between treatments (P <0.05). As result obtained from this study the highest mTORC1 and AKt gene expression were for T4 and T3, T2, T1 had higher gene expression compared to control respectively. In conclusion higher levels of energy are needed for Cobb 500, for both mTORC1 and AKt gene expression as well as the more carcass and breast muscle influences. |
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The effect of using different energy sources and levels on carcass weight, breast muscle mass, mTORC1 and AKt gene expression in Cobb 500 broiler chicksBreast muscle mass mTORC1, AKt gene expression in Cobb 500 broiler chicksThe present study was aimed to evaluate the effect of different energy sources and levels on carcass weight, breast muscle mass mTORC1 and AKt gene expression in Cobb 500 broiler chicks. A total of 600 1-day-old Cobb 500 broiler chicks with an average weight of 39 ± 0.50 g were randomly divided into five treatments. Each treatment was further divided into four replicates. Chicks were fed by basal diet based on corn and energy level by Cobb 500 instruction manual as control group, basal diet with 3% lesser energy than control (T1), basal diet with 6% lesser energy than control (T2), basal diet based on corn and fat level according to Cobb 500 instruction manual (T3), basal diet based on corn and fat with 3% upper energy (T4) for 42 days. To assess carcass characteristics two chicks of each replication were slaughtered and weighted after picking the carcasses. After DNA extraction, the UV spectrophotometric method was performed for determines the quality and quantity and Real-time PCR was performed on the corresponding cDNA synthesized from each sample. Data from this study showed that there were significant differences about carcass and breast muscle weight between treatments (P <0.05). As result obtained from this study the highest mTORC1 and AKt gene expression were for T4 and T3, T2, T1 had higher gene expression compared to control respectively. In conclusion higher levels of energy are needed for Cobb 500, for both mTORC1 and AKt gene expression as well as the more carcass and breast muscle influences.Revista Brasileira de Higiene e Sanidade Animal2023-01-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/68710.5935/rbhsa.v16i3.687Revista Brasileira de Higiene e Sanidade Animal; v. 16, n. 3 (2022); 1-13reponame:Revista brasileira de higiene e sanidade animalinstname:Universidade Federal do Ceará (UFC)instacron:UFCporhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/687/3348Akbari, Sayed Mostafa; Universidade Federal do Ceará, Fortaleza - CE - BrasilSadeghi, Ali Asghar; Islamic Azad University, Tehran, IranAminafshar, Mehdi; Islamic Azad University, Tehran, IranShawrang, Parvin; Islamic Azad University, Tehran, IranChamani, Mohammad; Institute, Atomic Energy Organization of Iraninfo:eu-repo/semantics/openAccess2023-01-13T00:45:37Zoai:ojs.www.higieneanimal.ufc.br:article/687Revistahttp://www.higieneanimal.ufc.br/PUBhttp://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/oaiwesleylyeverton@yahoo.com.br||ronaldo.sales@ufc.br1981-29651981-2965opendoar:2023-01-13T00:45:37Revista brasileira de higiene e sanidade animal - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
The effect of using different energy sources and levels on carcass weight, breast muscle mass, mTORC1 and AKt gene expression in Cobb 500 broiler chicks |
title |
The effect of using different energy sources and levels on carcass weight, breast muscle mass, mTORC1 and AKt gene expression in Cobb 500 broiler chicks |
spellingShingle |
The effect of using different energy sources and levels on carcass weight, breast muscle mass, mTORC1 and AKt gene expression in Cobb 500 broiler chicks Akbari, Sayed Mostafa; Universidade Federal do Ceará, Fortaleza - CE - Brasil Breast muscle mass mTORC1, AKt gene expression in Cobb 500 broiler chicks |
title_short |
The effect of using different energy sources and levels on carcass weight, breast muscle mass, mTORC1 and AKt gene expression in Cobb 500 broiler chicks |
title_full |
The effect of using different energy sources and levels on carcass weight, breast muscle mass, mTORC1 and AKt gene expression in Cobb 500 broiler chicks |
title_fullStr |
The effect of using different energy sources and levels on carcass weight, breast muscle mass, mTORC1 and AKt gene expression in Cobb 500 broiler chicks |
title_full_unstemmed |
The effect of using different energy sources and levels on carcass weight, breast muscle mass, mTORC1 and AKt gene expression in Cobb 500 broiler chicks |
title_sort |
The effect of using different energy sources and levels on carcass weight, breast muscle mass, mTORC1 and AKt gene expression in Cobb 500 broiler chicks |
author |
Akbari, Sayed Mostafa; Universidade Federal do Ceará, Fortaleza - CE - Brasil |
author_facet |
Akbari, Sayed Mostafa; Universidade Federal do Ceará, Fortaleza - CE - Brasil Sadeghi, Ali Asghar; Islamic Azad University, Tehran, Iran Aminafshar, Mehdi; Islamic Azad University, Tehran, Iran Shawrang, Parvin; Islamic Azad University, Tehran, Iran Chamani, Mohammad; Institute, Atomic Energy Organization of Iran |
author_role |
author |
author2 |
Sadeghi, Ali Asghar; Islamic Azad University, Tehran, Iran Aminafshar, Mehdi; Islamic Azad University, Tehran, Iran Shawrang, Parvin; Islamic Azad University, Tehran, Iran Chamani, Mohammad; Institute, Atomic Energy Organization of Iran |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Akbari, Sayed Mostafa; Universidade Federal do Ceará, Fortaleza - CE - Brasil Sadeghi, Ali Asghar; Islamic Azad University, Tehran, Iran Aminafshar, Mehdi; Islamic Azad University, Tehran, Iran Shawrang, Parvin; Islamic Azad University, Tehran, Iran Chamani, Mohammad; Institute, Atomic Energy Organization of Iran |
dc.subject.por.fl_str_mv |
Breast muscle mass mTORC1, AKt gene expression in Cobb 500 broiler chicks |
topic |
Breast muscle mass mTORC1, AKt gene expression in Cobb 500 broiler chicks |
description |
The present study was aimed to evaluate the effect of different energy sources and levels on carcass weight, breast muscle mass mTORC1 and AKt gene expression in Cobb 500 broiler chicks. A total of 600 1-day-old Cobb 500 broiler chicks with an average weight of 39 ± 0.50 g were randomly divided into five treatments. Each treatment was further divided into four replicates. Chicks were fed by basal diet based on corn and energy level by Cobb 500 instruction manual as control group, basal diet with 3% lesser energy than control (T1), basal diet with 6% lesser energy than control (T2), basal diet based on corn and fat level according to Cobb 500 instruction manual (T3), basal diet based on corn and fat with 3% upper energy (T4) for 42 days. To assess carcass characteristics two chicks of each replication were slaughtered and weighted after picking the carcasses. After DNA extraction, the UV spectrophotometric method was performed for determines the quality and quantity and Real-time PCR was performed on the corresponding cDNA synthesized from each sample. Data from this study showed that there were significant differences about carcass and breast muscle weight between treatments (P <0.05). As result obtained from this study the highest mTORC1 and AKt gene expression were for T4 and T3, T2, T1 had higher gene expression compared to control respectively. In conclusion higher levels of energy are needed for Cobb 500, for both mTORC1 and AKt gene expression as well as the more carcass and breast muscle influences. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-01-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/687 10.5935/rbhsa.v16i3.687 |
url |
http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/687 |
identifier_str_mv |
10.5935/rbhsa.v16i3.687 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/687/3348 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Revista Brasileira de Higiene e Sanidade Animal |
publisher.none.fl_str_mv |
Revista Brasileira de Higiene e Sanidade Animal |
dc.source.none.fl_str_mv |
Revista Brasileira de Higiene e Sanidade Animal; v. 16, n. 3 (2022); 1-13 reponame:Revista brasileira de higiene e sanidade animal instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Revista brasileira de higiene e sanidade animal |
collection |
Revista brasileira de higiene e sanidade animal |
repository.name.fl_str_mv |
Revista brasileira de higiene e sanidade animal - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
wesleylyeverton@yahoo.com.br||ronaldo.sales@ufc.br |
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1799695341522518016 |