Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows

Detalhes bibliográficos
Autor(a) principal: Pires, Bruno Cabral
Data de Publicação: 2018
Outros Autores: Grapiglia, Junia Berzin, Moreira, Lio, Jaeger, Lauren Hubert, Carvalho-Costa, Filipe Anibal, Lilenbaum, Walter
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/29691
Resumo: Reproductive tract is an important site of infection for chronic leptospirosis and cooperate in pathogenesis of reproductive failure, leading to economic losses. Since serology techniques cannot detect chronic carriers, the molecular analysis of clinical samples is an alternative to detect these animals on livestock. The aim of the present study was to perform a retrospective study in order to detect leptospiral uterine carriers in slaughtered cows. Tissue samples were collected from 50 post-pubertal, nonpregnant cows. These samples were fi xed in 10% bu ff ered formalin, para ffi n-embedded and stored. PCR targeting lip L32 gene and molecular characterization by sec Y sequence was performed. Leptospiral DNA was identi fi ed in 18% (9/50) examined blocks. Two sequences were characterized as L. interrogans . These fi ndings suggest that the presence of infectious leptospires in uterus is associated with the physiopathogenesis of the reproductive failure.
id UFC-7_01ab1527b7dd398e03c1620a945b341b
oai_identifier_str oai:repositorio.ufc.br:riufc/29691
network_acronym_str UFC-7
network_name_str Repositório Institucional da Universidade Federal do Ceará (UFC)
repository_id_str
spelling Occurrence of uterine carriers for Leptospira interrogans on slaughtered cowsLeptospiroseLeptospirosisÚteroReproductive tract is an important site of infection for chronic leptospirosis and cooperate in pathogenesis of reproductive failure, leading to economic losses. Since serology techniques cannot detect chronic carriers, the molecular analysis of clinical samples is an alternative to detect these animals on livestock. The aim of the present study was to perform a retrospective study in order to detect leptospiral uterine carriers in slaughtered cows. Tissue samples were collected from 50 post-pubertal, nonpregnant cows. These samples were fi xed in 10% bu ff ered formalin, para ffi n-embedded and stored. PCR targeting lip L32 gene and molecular characterization by sec Y sequence was performed. Leptospiral DNA was identi fi ed in 18% (9/50) examined blocks. Two sequences were characterized as L. interrogans . These fi ndings suggest that the presence of infectious leptospires in uterus is associated with the physiopathogenesis of the reproductive failure.Microbial Pathogenesis2018-02-15T17:42:07Z2018-02-15T17:42:07Z2018-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPIRES, B. C. et al. Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows. Microbial Pathogenesis, London, v. 114, p. 163-165, jan. 2018.0882-40101096-1208 (On line)http://www.repositorio.ufc.br/handle/riufc/29691Pires, Bruno CabralGrapiglia, Junia BerzinMoreira, LioJaeger, Lauren HubertCarvalho-Costa, Filipe AnibalLilenbaum, Walterengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-01-21T13:34:52Zoai:repositorio.ufc.br:riufc/29691Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:30:09.750805Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows
title Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows
spellingShingle Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows
Pires, Bruno Cabral
Leptospirose
Leptospirosis
Útero
title_short Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows
title_full Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows
title_fullStr Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows
title_full_unstemmed Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows
title_sort Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows
author Pires, Bruno Cabral
author_facet Pires, Bruno Cabral
Grapiglia, Junia Berzin
Moreira, Lio
Jaeger, Lauren Hubert
Carvalho-Costa, Filipe Anibal
Lilenbaum, Walter
author_role author
author2 Grapiglia, Junia Berzin
Moreira, Lio
Jaeger, Lauren Hubert
Carvalho-Costa, Filipe Anibal
Lilenbaum, Walter
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pires, Bruno Cabral
Grapiglia, Junia Berzin
Moreira, Lio
Jaeger, Lauren Hubert
Carvalho-Costa, Filipe Anibal
Lilenbaum, Walter
dc.subject.por.fl_str_mv Leptospirose
Leptospirosis
Útero
topic Leptospirose
Leptospirosis
Útero
description Reproductive tract is an important site of infection for chronic leptospirosis and cooperate in pathogenesis of reproductive failure, leading to economic losses. Since serology techniques cannot detect chronic carriers, the molecular analysis of clinical samples is an alternative to detect these animals on livestock. The aim of the present study was to perform a retrospective study in order to detect leptospiral uterine carriers in slaughtered cows. Tissue samples were collected from 50 post-pubertal, nonpregnant cows. These samples were fi xed in 10% bu ff ered formalin, para ffi n-embedded and stored. PCR targeting lip L32 gene and molecular characterization by sec Y sequence was performed. Leptospiral DNA was identi fi ed in 18% (9/50) examined blocks. Two sequences were characterized as L. interrogans . These fi ndings suggest that the presence of infectious leptospires in uterus is associated with the physiopathogenesis of the reproductive failure.
publishDate 2018
dc.date.none.fl_str_mv 2018-02-15T17:42:07Z
2018-02-15T17:42:07Z
2018-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv PIRES, B. C. et al. Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows. Microbial Pathogenesis, London, v. 114, p. 163-165, jan. 2018.
0882-4010
1096-1208 (On line)
http://www.repositorio.ufc.br/handle/riufc/29691
identifier_str_mv PIRES, B. C. et al. Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows. Microbial Pathogenesis, London, v. 114, p. 163-165, jan. 2018.
0882-4010
1096-1208 (On line)
url http://www.repositorio.ufc.br/handle/riufc/29691
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Microbial Pathogenesis
publisher.none.fl_str_mv Microbial Pathogenesis
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
_version_ 1813028830838784000

Registros relacionados