Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar Guaricura, from serogroup Sejroe

Detalhes bibliográficos
Autor(a) principal: Barbosa, Cristina
Data de Publicação: 2023
Outros Autores: Oliveira, Anderson dos Santos, Mendes, Julia Santos, Ristow, Paula, Lilenbaum, Walter
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/60695
Resumo: Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro. Conselho Nacional de Desenvolvimento Científico e Tecnológico.
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spelling Barbosa, CristinaOliveira, Anderson dos SantosMendes, Julia SantosRistow, PaulaLilenbaum, Walter2023-10-04T19:01:22Z2023-10-04T19:01:22Z2023BARBOSA, Cristina et al. Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar guaricura, from serogroup sejroe. Veterinary Research Communications, p. 1-5, 2023.1573-7446https://www.arca.fiocruz.br/handle/icict/6069510.1007/s11259-023-10108-yFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro. Conselho Nacional de Desenvolvimento Científico e Tecnológico.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil / Universidade Federal da Bahia. Instituto de Biologia. Laboratório de Bacteriologia e Saúde. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Biologia. Instituto Nacional de Ciência e Tecnologia em Estudos Interdisciplinares e Transdisciplinares em Ecologia e Evolução. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.Leptospirosis is an important zoonosis that in cattle is characterized as a reproductive disease. It is well reported that the main agent of bovine leptospirosis worldwide is Sejroe serogroup serovar Hardjo. Reproductive disease in cattle has several gaps in its knowledge and studies with Golden Syrian hamsters, experimentally infected, are limited. Therefore, a protocol that could reproduce the chronic genital disease in hamsters would be extremely valuable for the advance of the knowledge of that syndrome. The aim of this study was to establish an experimental protocol for chronic non-lethal genital infection of female hamsters by L. santarosai serovar Guaricura (Sejroe serogroup), strain 2013_VF52. For this, two concentrations (1.0 × 108 leptospires/mL and 1.0 × 104 leptospires/mL) were used intraperitoneally in female hamsters of 06–08 weeks of age. Hamsters that survived for up to forty days after inoculation were euthanized. Uterine and renal tissues were collected to evaluate leptospires’ presence by PCR and culture. The protocol demonstrated that 1.0 × 104 leptospires/mL of the strain determined chronic genital leptospirosis in the hamster model. The standardization of a protocol for chronic genital leptospirosis in hamsters can be extremely useful for the understanding of the physiopathology of the infection, as the distribution of leptospires in the uterus and the agent-host interactions.engSpringerDoença crônicaÚteroHamsterLeptospiroseChronic diseaseUterusHamsterLeptospirosisDoença crônicaÚteroLeptospiroseStandardization of chronic uterine infection of hamsters by leptospira santarosai serovar Guaricura, from serogroup Sejroeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/60695/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBarbosa, Cristina - Standardization of chronic uterine infection of hamsters by Leptospira santarosai serovar Guaricura, from serogroup Sejroe.pdfBarbosa, Cristina - Standardization of chronic uterine infection of hamsters by Leptospira santarosai serovar Guaricura, from serogroup Sejroe.pdfapplication/pdf847431https://www.arca.fiocruz.br/bitstream/icict/60695/2/Barbosa%2c%20Cristina%20-%20Standardization%20of%20chronic%20uterine%20infection%20of%20hamsters%20by%20Leptospira%20santarosai%20serovar%20Guaricura%2c%20from%20serogroup%20Sejroe.pdf9de03803b060804e220844bd0245a801MD52icict/606952023-10-04 16:01:23.418oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-10-04T19:01:23Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.en_US.fl_str_mv Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar Guaricura, from serogroup Sejroe
title Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar Guaricura, from serogroup Sejroe
spellingShingle Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar Guaricura, from serogroup Sejroe
Barbosa, Cristina
Doença crônica
Útero
Hamster
Leptospirose
Chronic disease
Uterus
Hamster
Leptospirosis
Doença crônica
Útero
Leptospirose
title_short Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar Guaricura, from serogroup Sejroe
title_full Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar Guaricura, from serogroup Sejroe
title_fullStr Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar Guaricura, from serogroup Sejroe
title_full_unstemmed Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar Guaricura, from serogroup Sejroe
title_sort Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar Guaricura, from serogroup Sejroe
author Barbosa, Cristina
author_facet Barbosa, Cristina
Oliveira, Anderson dos Santos
Mendes, Julia Santos
Ristow, Paula
Lilenbaum, Walter
author_role author
author2 Oliveira, Anderson dos Santos
Mendes, Julia Santos
Ristow, Paula
Lilenbaum, Walter
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, Cristina
Oliveira, Anderson dos Santos
Mendes, Julia Santos
Ristow, Paula
Lilenbaum, Walter
dc.subject.other.en_US.fl_str_mv Doença crônica
Útero
Hamster
Leptospirose
topic Doença crônica
Útero
Hamster
Leptospirose
Chronic disease
Uterus
Hamster
Leptospirosis
Doença crônica
Útero
Leptospirose
dc.subject.en.en_US.fl_str_mv Chronic disease
Uterus
Hamster
Leptospirosis
dc.subject.decs.en_US.fl_str_mv Doença crônica
Útero
Leptospirose
description Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro. Conselho Nacional de Desenvolvimento Científico e Tecnológico.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-10-04T19:01:22Z
dc.date.available.fl_str_mv 2023-10-04T19:01:22Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA, Cristina et al. Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar guaricura, from serogroup sejroe. Veterinary Research Communications, p. 1-5, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/60695
dc.identifier.issn.en_US.fl_str_mv 1573-7446
dc.identifier.doi.none.fl_str_mv 10.1007/s11259-023-10108-y
identifier_str_mv BARBOSA, Cristina et al. Standardization of chronic uterine infection of hamsters by leptospira santarosai serovar guaricura, from serogroup sejroe. Veterinary Research Communications, p. 1-5, 2023.
1573-7446
10.1007/s11259-023-10108-y
url https://www.arca.fiocruz.br/handle/icict/60695
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/60695/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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