Effects of ketamine in methicillin-resistant Staphylococcus aureus and in silico interaction with sortase A

• Autor(a) principal: Coutinho, Tatiana do Nascimento Paiva
• Data de Publicação: 2021
• Outros Autores: Barroso, Fátima Daiana Dias, Silva, Cecília Rocha da, Silva, Anderson Ramos da, Cabral, Vitória Pessoa de Farias, Sá, Lívia Gurgel do Amaral Valente, Cândido, Thiago Mesquita, Silva, Lisandra Juvencio da, Ferreira, Thais Lima, Silva, Wildson Max Barbosa da, Marinho, Emmanuel Silva, Cavalcanti, Bruno Coelho, Moraes, Manoel Odorico de, Andrade Neto, João Batista de, Silva, Jacilene
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/61923
• topic: Staphylococcus Aureus Resistente à Meticilina; Methicillin-Resistant Staphylococcus Aureus; Ketamine; Ketamina; Flow Cytometry; Citometria de Fluxo; Docking Molecular; Acoplamento Molecular

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main human pathogens and is responsible for many diseases, ranging from skin infections to more invasive infections. These infections are dangerous and expensive to treat because these strains are resistant to a large number of conventional antibiotics. Thus, the antibacterial effect of ketamine against MRSA strains, its mechanism of action, and in silico interaction with sortase A were evaluated. The antibacterial effect of ketamine was assessed using the broth microdilution method. Subsequently, the mechanism of action was assessed using flow cytometry and molecular docking assays with sortase A. Our results showed that ketamine has a significant antibacterial activity against MRSA strains in the range of 2.49–3.73 mM. Their mechanism of action involves alterations in membrane integrity and DNA damage, reducing cell viability, and inducing apoptosis. In addition, ketamine had an affinity for S. aureus sortase A. These results indicate that this compound can be used as an alternative to develop new strategies to combat infections caused by MRSA.