Development of a subcutaneous endometriosis rat model
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/18140 |
Resumo: | PURPOSE: To present a rat model of subcutaneous endometriosis for the study of pathophysiology and the effects of drugs. METHODS: Fifty three-month-old female Wistar rats (Rattus norvergicus) were distributed into one control group and four treatment groups: estradiol (2.5; 5; 10mg/kg sc), medroxyprogesterone acetate (0.5; 2; 5mg/kg sc), triptorelin pamoate (0.18; 0.56mg/kg sc) and acetylsalicylic acid (3mg/kg per os). The animals were autoimplanted subcutaneously with 4x4-mm uterine fragments to induce endometriosis. The endometriomas were measured on days 1, 7, 14 and 21. The relative dry and wet weights of the endometrioma were used to evaluate response to the drug. Endometrial -like tissue was confirmed by histology. The greatest weight gain was observed on day 14 (relative wet weight: 29.1 ± 6.7mg%, relative dry weight: 5.3 ± 0.9mg %). Treatments were administered between day 5 and day 14. RESULTS: The relative wet weight of the hemiuterus in the 10mg/kg estradiol group differed significantly from control and the other two estradiol groups (p=0.0001). In the medroxyprogesterone acetate group the weight decreased significantly but this decrease was not dose-dependent. Weight reduction was also significant in the triptorelin pamoate and the acetylsalicylic acid groups. CONCLUSION: The model of subcutaneous endometriosis is reproducible, low-cost and easy to perform, and suitable for the study of pathophysiology and the effects of drugs. |
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Repositório Institucional da Universidade Federal do Ceará (UFC) |
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Development of a subcutaneous endometriosis rat modelEndometriosePamoato de TriptorrelinaPURPOSE: To present a rat model of subcutaneous endometriosis for the study of pathophysiology and the effects of drugs. METHODS: Fifty three-month-old female Wistar rats (Rattus norvergicus) were distributed into one control group and four treatment groups: estradiol (2.5; 5; 10mg/kg sc), medroxyprogesterone acetate (0.5; 2; 5mg/kg sc), triptorelin pamoate (0.18; 0.56mg/kg sc) and acetylsalicylic acid (3mg/kg per os). The animals were autoimplanted subcutaneously with 4x4-mm uterine fragments to induce endometriosis. The endometriomas were measured on days 1, 7, 14 and 21. The relative dry and wet weights of the endometrioma were used to evaluate response to the drug. Endometrial -like tissue was confirmed by histology. The greatest weight gain was observed on day 14 (relative wet weight: 29.1 ± 6.7mg%, relative dry weight: 5.3 ± 0.9mg %). Treatments were administered between day 5 and day 14. RESULTS: The relative wet weight of the hemiuterus in the 10mg/kg estradiol group differed significantly from control and the other two estradiol groups (p=0.0001). In the medroxyprogesterone acetate group the weight decreased significantly but this decrease was not dose-dependent. Weight reduction was also significant in the triptorelin pamoate and the acetylsalicylic acid groups. CONCLUSION: The model of subcutaneous endometriosis is reproducible, low-cost and easy to perform, and suitable for the study of pathophysiology and the effects of drugs.Acta Cirúrgica Brasileira2016-07-05T11:21:16Z2016-07-05T11:21:16Z2015-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPEREIRA, F. E. X. G. et al. Development of a subcutaneous endometriosis rat model. Acta Cirúrgica Brasileira, São Paulo, v. 30, n. 1, p. 1-7, jan. 2015.On-line version 1678-2674http://www.repositorio.ufc.br/handle/riufc/18140Pereira, Francisco Edson Ximenes GomesAlmeida, Paulo Roberto Carvalho deDias, Bruno Hállan MenesesVasconcelos, Paulo Roberto Leitão deGuimarães, Sérgio Botelho GuimarãesMedeiros, Francisco das Chagasengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2018-12-17T11:11:22Zoai:repositorio.ufc.br:riufc/18140Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2018-12-17T11:11:22Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Development of a subcutaneous endometriosis rat model |
title |
Development of a subcutaneous endometriosis rat model |
spellingShingle |
Development of a subcutaneous endometriosis rat model Pereira, Francisco Edson Ximenes Gomes Endometriose Pamoato de Triptorrelina |
title_short |
Development of a subcutaneous endometriosis rat model |
title_full |
Development of a subcutaneous endometriosis rat model |
title_fullStr |
Development of a subcutaneous endometriosis rat model |
title_full_unstemmed |
Development of a subcutaneous endometriosis rat model |
title_sort |
Development of a subcutaneous endometriosis rat model |
author |
Pereira, Francisco Edson Ximenes Gomes |
author_facet |
Pereira, Francisco Edson Ximenes Gomes Almeida, Paulo Roberto Carvalho de Dias, Bruno Hállan Meneses Vasconcelos, Paulo Roberto Leitão de Guimarães, Sérgio Botelho Guimarães Medeiros, Francisco das Chagas |
author_role |
author |
author2 |
Almeida, Paulo Roberto Carvalho de Dias, Bruno Hállan Meneses Vasconcelos, Paulo Roberto Leitão de Guimarães, Sérgio Botelho Guimarães Medeiros, Francisco das Chagas |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pereira, Francisco Edson Ximenes Gomes Almeida, Paulo Roberto Carvalho de Dias, Bruno Hállan Meneses Vasconcelos, Paulo Roberto Leitão de Guimarães, Sérgio Botelho Guimarães Medeiros, Francisco das Chagas |
dc.subject.por.fl_str_mv |
Endometriose Pamoato de Triptorrelina |
topic |
Endometriose Pamoato de Triptorrelina |
description |
PURPOSE: To present a rat model of subcutaneous endometriosis for the study of pathophysiology and the effects of drugs. METHODS: Fifty three-month-old female Wistar rats (Rattus norvergicus) were distributed into one control group and four treatment groups: estradiol (2.5; 5; 10mg/kg sc), medroxyprogesterone acetate (0.5; 2; 5mg/kg sc), triptorelin pamoate (0.18; 0.56mg/kg sc) and acetylsalicylic acid (3mg/kg per os). The animals were autoimplanted subcutaneously with 4x4-mm uterine fragments to induce endometriosis. The endometriomas were measured on days 1, 7, 14 and 21. The relative dry and wet weights of the endometrioma were used to evaluate response to the drug. Endometrial -like tissue was confirmed by histology. The greatest weight gain was observed on day 14 (relative wet weight: 29.1 ± 6.7mg%, relative dry weight: 5.3 ± 0.9mg %). Treatments were administered between day 5 and day 14. RESULTS: The relative wet weight of the hemiuterus in the 10mg/kg estradiol group differed significantly from control and the other two estradiol groups (p=0.0001). In the medroxyprogesterone acetate group the weight decreased significantly but this decrease was not dose-dependent. Weight reduction was also significant in the triptorelin pamoate and the acetylsalicylic acid groups. CONCLUSION: The model of subcutaneous endometriosis is reproducible, low-cost and easy to perform, and suitable for the study of pathophysiology and the effects of drugs. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01 2016-07-05T11:21:16Z 2016-07-05T11:21:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PEREIRA, F. E. X. G. et al. Development of a subcutaneous endometriosis rat model. Acta Cirúrgica Brasileira, São Paulo, v. 30, n. 1, p. 1-7, jan. 2015. On-line version 1678-2674 http://www.repositorio.ufc.br/handle/riufc/18140 |
identifier_str_mv |
PEREIRA, F. E. X. G. et al. Development of a subcutaneous endometriosis rat model. Acta Cirúrgica Brasileira, São Paulo, v. 30, n. 1, p. 1-7, jan. 2015. On-line version 1678-2674 |
url |
http://www.repositorio.ufc.br/handle/riufc/18140 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Acta Cirúrgica Brasileira |
publisher.none.fl_str_mv |
Acta Cirúrgica Brasileira |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1809935779154100224 |