Development of a subcutaneous endometriosis rat model

Detalhes bibliográficos
Autor(a) principal: Pereira, Francisco Edson Ximenes Gomes
Data de Publicação: 2015
Outros Autores: Almeida, Paulo Roberto Carvalho de, Dias, Bruno Hállan Meneses, Vasconcelos, Paulo Roberto Leitão de, Guimarães, Sérgio Botelho Guimarães, Medeiros, Francisco das Chagas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
dARK ID: ark:/83112/001300000qpqr
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/18140
Resumo: PURPOSE: To present a rat model of subcutaneous endometriosis for the study of pathophysiology and the effects of drugs. METHODS: Fifty three-month-old female Wistar rats (Rattus norvergicus) were distributed into one control group and four treatment groups: estradiol (2.5; 5; 10mg/kg sc), medroxyprogesterone acetate (0.5; 2; 5mg/kg sc), triptorelin pamoate (0.18; 0.56mg/kg sc) and acetylsalicylic acid (3mg/kg per os). The animals were autoimplanted subcutaneously with 4x4-mm uterine fragments to induce endometriosis. The endometriomas were measured on days 1, 7, 14 and 21. The relative dry and wet weights of the endometrioma were used to evaluate response to the drug. Endometrial -like tissue was confirmed by histology. The greatest weight gain was observed on day 14 (relative wet weight: 29.1 ± 6.7mg%, relative dry weight: 5.3 ± 0.9mg %). Treatments were administered between day 5 and day 14. RESULTS: The relative wet weight of the hemiuterus in the 10mg/kg estradiol group differed significantly from control and the other two estradiol groups (p=0.0001). In the medroxyprogesterone acetate group the weight decreased significantly but this decrease was not dose-dependent. Weight reduction was also significant in the triptorelin pamoate and the acetylsalicylic acid groups. CONCLUSION: The model of subcutaneous endometriosis is reproducible, low-cost and easy to perform, and suitable for the study of pathophysiology and the effects of drugs.
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spelling Development of a subcutaneous endometriosis rat modelEndometriosePamoato de TriptorrelinaPURPOSE: To present a rat model of subcutaneous endometriosis for the study of pathophysiology and the effects of drugs. METHODS: Fifty three-month-old female Wistar rats (Rattus norvergicus) were distributed into one control group and four treatment groups: estradiol (2.5; 5; 10mg/kg sc), medroxyprogesterone acetate (0.5; 2; 5mg/kg sc), triptorelin pamoate (0.18; 0.56mg/kg sc) and acetylsalicylic acid (3mg/kg per os). The animals were autoimplanted subcutaneously with 4x4-mm uterine fragments to induce endometriosis. The endometriomas were measured on days 1, 7, 14 and 21. The relative dry and wet weights of the endometrioma were used to evaluate response to the drug. Endometrial -like tissue was confirmed by histology. The greatest weight gain was observed on day 14 (relative wet weight: 29.1 ± 6.7mg%, relative dry weight: 5.3 ± 0.9mg %). Treatments were administered between day 5 and day 14. RESULTS: The relative wet weight of the hemiuterus in the 10mg/kg estradiol group differed significantly from control and the other two estradiol groups (p=0.0001). In the medroxyprogesterone acetate group the weight decreased significantly but this decrease was not dose-dependent. Weight reduction was also significant in the triptorelin pamoate and the acetylsalicylic acid groups. CONCLUSION: The model of subcutaneous endometriosis is reproducible, low-cost and easy to perform, and suitable for the study of pathophysiology and the effects of drugs.Acta Cirúrgica Brasileira2016-07-05T11:21:16Z2016-07-05T11:21:16Z2015-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPEREIRA, F. E. X. G. et al. Development of a subcutaneous endometriosis rat model. Acta Cirúrgica Brasileira, São Paulo, v. 30, n. 1, p. 1-7, jan. 2015.On-line version 1678-2674http://www.repositorio.ufc.br/handle/riufc/18140ark:/83112/001300000qpqrPereira, Francisco Edson Ximenes GomesAlmeida, Paulo Roberto Carvalho deDias, Bruno Hállan MenesesVasconcelos, Paulo Roberto Leitão deGuimarães, Sérgio Botelho GuimarãesMedeiros, Francisco das Chagasengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2018-12-17T11:11:22Zoai:repositorio.ufc.br:riufc/18140Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:15:48.291040Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Development of a subcutaneous endometriosis rat model
title Development of a subcutaneous endometriosis rat model
spellingShingle Development of a subcutaneous endometriosis rat model
Pereira, Francisco Edson Ximenes Gomes
Endometriose
Pamoato de Triptorrelina
title_short Development of a subcutaneous endometriosis rat model
title_full Development of a subcutaneous endometriosis rat model
title_fullStr Development of a subcutaneous endometriosis rat model
title_full_unstemmed Development of a subcutaneous endometriosis rat model
title_sort Development of a subcutaneous endometriosis rat model
author Pereira, Francisco Edson Ximenes Gomes
author_facet Pereira, Francisco Edson Ximenes Gomes
Almeida, Paulo Roberto Carvalho de
Dias, Bruno Hállan Meneses
Vasconcelos, Paulo Roberto Leitão de
Guimarães, Sérgio Botelho Guimarães
Medeiros, Francisco das Chagas
author_role author
author2 Almeida, Paulo Roberto Carvalho de
Dias, Bruno Hállan Meneses
Vasconcelos, Paulo Roberto Leitão de
Guimarães, Sérgio Botelho Guimarães
Medeiros, Francisco das Chagas
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Francisco Edson Ximenes Gomes
Almeida, Paulo Roberto Carvalho de
Dias, Bruno Hállan Meneses
Vasconcelos, Paulo Roberto Leitão de
Guimarães, Sérgio Botelho Guimarães
Medeiros, Francisco das Chagas
dc.subject.por.fl_str_mv Endometriose
Pamoato de Triptorrelina
topic Endometriose
Pamoato de Triptorrelina
description PURPOSE: To present a rat model of subcutaneous endometriosis for the study of pathophysiology and the effects of drugs. METHODS: Fifty three-month-old female Wistar rats (Rattus norvergicus) were distributed into one control group and four treatment groups: estradiol (2.5; 5; 10mg/kg sc), medroxyprogesterone acetate (0.5; 2; 5mg/kg sc), triptorelin pamoate (0.18; 0.56mg/kg sc) and acetylsalicylic acid (3mg/kg per os). The animals were autoimplanted subcutaneously with 4x4-mm uterine fragments to induce endometriosis. The endometriomas were measured on days 1, 7, 14 and 21. The relative dry and wet weights of the endometrioma were used to evaluate response to the drug. Endometrial -like tissue was confirmed by histology. The greatest weight gain was observed on day 14 (relative wet weight: 29.1 ± 6.7mg%, relative dry weight: 5.3 ± 0.9mg %). Treatments were administered between day 5 and day 14. RESULTS: The relative wet weight of the hemiuterus in the 10mg/kg estradiol group differed significantly from control and the other two estradiol groups (p=0.0001). In the medroxyprogesterone acetate group the weight decreased significantly but this decrease was not dose-dependent. Weight reduction was also significant in the triptorelin pamoate and the acetylsalicylic acid groups. CONCLUSION: The model of subcutaneous endometriosis is reproducible, low-cost and easy to perform, and suitable for the study of pathophysiology and the effects of drugs.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
2016-07-05T11:21:16Z
2016-07-05T11:21:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv PEREIRA, F. E. X. G. et al. Development of a subcutaneous endometriosis rat model. Acta Cirúrgica Brasileira, São Paulo, v. 30, n. 1, p. 1-7, jan. 2015.
On-line version 1678-2674
http://www.repositorio.ufc.br/handle/riufc/18140
dc.identifier.dark.fl_str_mv ark:/83112/001300000qpqr
identifier_str_mv PEREIRA, F. E. X. G. et al. Development of a subcutaneous endometriosis rat model. Acta Cirúrgica Brasileira, São Paulo, v. 30, n. 1, p. 1-7, jan. 2015.
On-line version 1678-2674
ark:/83112/001300000qpqr
url http://www.repositorio.ufc.br/handle/riufc/18140
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Acta Cirúrgica Brasileira
publisher.none.fl_str_mv Acta Cirúrgica Brasileira
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
_version_ 1818373818747453440

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