Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/7253 |
Resumo: | This study aimed to evaluate the mechanism associated with cytotoxic activity displayed by the drug 5- fluorouracil incorporated in Cu-BTC MOF and its slow delivery from the Cu-BTC MOF. Structural characterization encompasses elemental analysis (CHNS), differential scanning calorimetry (DSC), thermogravimetric analysis (TG/DTG), Fournier transform infrared (FIT-IR) and X-ray diffraction (XRD) was performed to verify the process of association between the drug 5-FU and Cu-BTC MOF. Flow cytometry was done to indicate that apoptosis is the mechanism responsible for the cell death. The release profile of the drug 5-FU from Cu-BTC MOF for 48 hours was obeisant. Also, the anti-inflammatory activity was evaluated by the peritonitis testing and the production of nitric oxide and pro-inflammatory cytokines were measured. The chemical characterization of the material indicated the presence of drug associated with the coordination network in a proportion of 0.82 g 5-FU per 1.0 g of Cu-BTC MOF. The cytotoxic tests were carried out against four cell lines: NCI-H292, MCF-7, HT29 and HL60. The Cu-BTC MOF associated drug was extremely cytotoxic against the human breast cancer adenocarcinoma (MCF- 7) cell line and against human acute promyelocytic leukemia cells (HL60), cancer cells were killed by apoptosis mechanisms. The drug demonstrated a slow release profile where 82% of the drug was released in 48 hours. The results indicated that the drug incorporated in Cu-BTC MOF decreased significantly the number of leukocytes in the peritoneal cavity of rodents as well as reduced levels of cytokines and nitric oxide production. |
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Repositório Institucional da Universidade Federal do Ceará (UFC) |
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Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTCApoptoseCancroMorte CelularThis study aimed to evaluate the mechanism associated with cytotoxic activity displayed by the drug 5- fluorouracil incorporated in Cu-BTC MOF and its slow delivery from the Cu-BTC MOF. Structural characterization encompasses elemental analysis (CHNS), differential scanning calorimetry (DSC), thermogravimetric analysis (TG/DTG), Fournier transform infrared (FIT-IR) and X-ray diffraction (XRD) was performed to verify the process of association between the drug 5-FU and Cu-BTC MOF. Flow cytometry was done to indicate that apoptosis is the mechanism responsible for the cell death. The release profile of the drug 5-FU from Cu-BTC MOF for 48 hours was obeisant. Also, the anti-inflammatory activity was evaluated by the peritonitis testing and the production of nitric oxide and pro-inflammatory cytokines were measured. The chemical characterization of the material indicated the presence of drug associated with the coordination network in a proportion of 0.82 g 5-FU per 1.0 g of Cu-BTC MOF. The cytotoxic tests were carried out against four cell lines: NCI-H292, MCF-7, HT29 and HL60. The Cu-BTC MOF associated drug was extremely cytotoxic against the human breast cancer adenocarcinoma (MCF- 7) cell line and against human acute promyelocytic leukemia cells (HL60), cancer cells were killed by apoptosis mechanisms. The drug demonstrated a slow release profile where 82% of the drug was released in 48 hours. The results indicated that the drug incorporated in Cu-BTC MOF decreased significantly the number of leukocytes in the peritoneal cavity of rodents as well as reduced levels of cytokines and nitric oxide production.Biomedicine & Pharmacotherapy2014-02-13T15:56:20Z2014-02-13T15:56:20Z2013-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLUCENA, F. R. S. et al. Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC. Biomedicine & Pharmacotherapy, Paris, v. 67, n. 8, p. 707-713, out. 2013.0753-3322 Impresso1950-6007 On linehttp://www.repositorio.ufc.br/handle/riufc/7253Lucena, Flávia Raquel SantosAraújo, Larissa C.C. deRodrigues, Maria do D.Silva, Teresinha G. daPereira, Valéria R.A.Militão, Gardênia C.G.Fontes, Danilo A.F.Rolim-Neto, Pedro J.Silva, Fausthon F. daNascimento, Silene C.engreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-01-15T15:50:06Zoai:repositorio.ufc.br:riufc/7253Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2019-01-15T15:50:06Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC |
title |
Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC |
spellingShingle |
Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC Lucena, Flávia Raquel Santos Apoptose Cancro Morte Celular |
title_short |
Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC |
title_full |
Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC |
title_fullStr |
Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC |
title_full_unstemmed |
Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC |
title_sort |
Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC |
author |
Lucena, Flávia Raquel Santos |
author_facet |
Lucena, Flávia Raquel Santos Araújo, Larissa C.C. de Rodrigues, Maria do D. Silva, Teresinha G. da Pereira, Valéria R.A. Militão, Gardênia C.G. Fontes, Danilo A.F. Rolim-Neto, Pedro J. Silva, Fausthon F. da Nascimento, Silene C. |
author_role |
author |
author2 |
Araújo, Larissa C.C. de Rodrigues, Maria do D. Silva, Teresinha G. da Pereira, Valéria R.A. Militão, Gardênia C.G. Fontes, Danilo A.F. Rolim-Neto, Pedro J. Silva, Fausthon F. da Nascimento, Silene C. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lucena, Flávia Raquel Santos Araújo, Larissa C.C. de Rodrigues, Maria do D. Silva, Teresinha G. da Pereira, Valéria R.A. Militão, Gardênia C.G. Fontes, Danilo A.F. Rolim-Neto, Pedro J. Silva, Fausthon F. da Nascimento, Silene C. |
dc.subject.por.fl_str_mv |
Apoptose Cancro Morte Celular |
topic |
Apoptose Cancro Morte Celular |
description |
This study aimed to evaluate the mechanism associated with cytotoxic activity displayed by the drug 5- fluorouracil incorporated in Cu-BTC MOF and its slow delivery from the Cu-BTC MOF. Structural characterization encompasses elemental analysis (CHNS), differential scanning calorimetry (DSC), thermogravimetric analysis (TG/DTG), Fournier transform infrared (FIT-IR) and X-ray diffraction (XRD) was performed to verify the process of association between the drug 5-FU and Cu-BTC MOF. Flow cytometry was done to indicate that apoptosis is the mechanism responsible for the cell death. The release profile of the drug 5-FU from Cu-BTC MOF for 48 hours was obeisant. Also, the anti-inflammatory activity was evaluated by the peritonitis testing and the production of nitric oxide and pro-inflammatory cytokines were measured. The chemical characterization of the material indicated the presence of drug associated with the coordination network in a proportion of 0.82 g 5-FU per 1.0 g of Cu-BTC MOF. The cytotoxic tests were carried out against four cell lines: NCI-H292, MCF-7, HT29 and HL60. The Cu-BTC MOF associated drug was extremely cytotoxic against the human breast cancer adenocarcinoma (MCF- 7) cell line and against human acute promyelocytic leukemia cells (HL60), cancer cells were killed by apoptosis mechanisms. The drug demonstrated a slow release profile where 82% of the drug was released in 48 hours. The results indicated that the drug incorporated in Cu-BTC MOF decreased significantly the number of leukocytes in the peritoneal cavity of rodents as well as reduced levels of cytokines and nitric oxide production. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10 2014-02-13T15:56:20Z 2014-02-13T15:56:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
LUCENA, F. R. S. et al. Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC. Biomedicine & Pharmacotherapy, Paris, v. 67, n. 8, p. 707-713, out. 2013. 0753-3322 Impresso 1950-6007 On line http://www.repositorio.ufc.br/handle/riufc/7253 |
identifier_str_mv |
LUCENA, F. R. S. et al. Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC. Biomedicine & Pharmacotherapy, Paris, v. 67, n. 8, p. 707-713, out. 2013. 0753-3322 Impresso 1950-6007 On line |
url |
http://www.repositorio.ufc.br/handle/riufc/7253 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Biomedicine & Pharmacotherapy |
publisher.none.fl_str_mv |
Biomedicine & Pharmacotherapy |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1809935802071777280 |