In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13

Detalhes bibliográficos
Autor(a) principal: Coêlho, Zirlane Castelo B
Data de Publicação: 2010
Outros Autores: Teixeira, Maria Jania, Mota, Erika Freitas, Frutuoso, Mércia Sindeaux, Silva, João Santana da, Barral, Aldina, Barral Netto, Manoel, Pompeu, Margarida Maria L
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/63120
Resumo: The initial encounter of Leishmania with its host’s immune system is important in the outcome of infection. Previous studies have shown that PBMCs from healthy volunteers (HV) exposed to Leishmania differ in IFN-γ production. We have expanded such observations evaluating the profi le and kinetics of cytokines (IFN-γ, IL-12p70, IL-10, IL-13), chemokines (CCL5, CCL3, CCL4, CXCL10), and chemokine receptors (CCR1,CCR5, CXCR3, CCR4) in vitro L. amazonensis-stimulated of HV´s PBMCs. HVs were divided in groups of high (HR) or low (LR) IFN-γ responders. In both groups, HR and LR, after L. amazonensis infection there was a predominance of IL-10 and IL-13 over IFN-γ production, while IL-12 was produced in similar amount. Regarding chemokines, a more striking difference was observed for CCL3 expression that was lower at 12 hours and 48 hours post infection in LR than in HR. Interestingly, a downregulation of CCR5 and a greater expression of CCR4 were found in low IFN-γ responders. These data suggest that early after L. amazonensis infection there is a cytokine milieu dominated by IL-13 and IL-10, and despite of this environment, IFN-γ is produced, supporting the complexity of the response. It is noteworthy that the pattern of immune response is mounted in fi rst hours after Leishmania stimulation, with the defi nition of the differentiation of Th1 versus Th2 cells. It remains to be determined if such an in vitro difference has an in vivo counterpart in terms of susceptibility to infection
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spelling In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13Leishmania amazonensisInterleukin-10Interleukin-13The initial encounter of Leishmania with its host’s immune system is important in the outcome of infection. Previous studies have shown that PBMCs from healthy volunteers (HV) exposed to Leishmania differ in IFN-γ production. We have expanded such observations evaluating the profi le and kinetics of cytokines (IFN-γ, IL-12p70, IL-10, IL-13), chemokines (CCL5, CCL3, CCL4, CXCL10), and chemokine receptors (CCR1,CCR5, CXCR3, CCR4) in vitro L. amazonensis-stimulated of HV´s PBMCs. HVs were divided in groups of high (HR) or low (LR) IFN-γ responders. In both groups, HR and LR, after L. amazonensis infection there was a predominance of IL-10 and IL-13 over IFN-γ production, while IL-12 was produced in similar amount. Regarding chemokines, a more striking difference was observed for CCL3 expression that was lower at 12 hours and 48 hours post infection in LR than in HR. Interestingly, a downregulation of CCR5 and a greater expression of CCR4 were found in low IFN-γ responders. These data suggest that early after L. amazonensis infection there is a cytokine milieu dominated by IL-13 and IL-10, and despite of this environment, IFN-γ is produced, supporting the complexity of the response. It is noteworthy that the pattern of immune response is mounted in fi rst hours after Leishmania stimulation, with the defi nition of the differentiation of Th1 versus Th2 cells. It remains to be determined if such an in vitro difference has an in vivo counterpart in terms of susceptibility to infectionThe Brazilian Journal of Infectious Diseases2021-12-20T14:46:57Z2021-12-20T14:46:57Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCOÊLHO, Zirlane Castelo B et al. In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13. The Brazilian Journal of Infectious Diseases, [s. l.], v. 14, p. 478-484, 2010.http://www.repositorio.ufc.br/handle/riufc/63120Coêlho, Zirlane Castelo BTeixeira, Maria JaniaMota, Erika FreitasFrutuoso, Mércia SindeauxSilva, João Santana daBarral, AldinaBarral Netto, ManoelPompeu, Margarida Maria Linfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFC2023-10-10T19:38:21Zoai:repositorio.ufc.br:riufc/63120Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:45:25.153093Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
title In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
spellingShingle In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
Coêlho, Zirlane Castelo B
Leishmania amazonensis
Interleukin-10
Interleukin-13
title_short In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
title_full In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
title_fullStr In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
title_full_unstemmed In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
title_sort In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
author Coêlho, Zirlane Castelo B
author_facet Coêlho, Zirlane Castelo B
Teixeira, Maria Jania
Mota, Erika Freitas
Frutuoso, Mércia Sindeaux
Silva, João Santana da
Barral, Aldina
Barral Netto, Manoel
Pompeu, Margarida Maria L
author_role author
author2 Teixeira, Maria Jania
Mota, Erika Freitas
Frutuoso, Mércia Sindeaux
Silva, João Santana da
Barral, Aldina
Barral Netto, Manoel
Pompeu, Margarida Maria L
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Coêlho, Zirlane Castelo B
Teixeira, Maria Jania
Mota, Erika Freitas
Frutuoso, Mércia Sindeaux
Silva, João Santana da
Barral, Aldina
Barral Netto, Manoel
Pompeu, Margarida Maria L
dc.subject.por.fl_str_mv Leishmania amazonensis
Interleukin-10
Interleukin-13
topic Leishmania amazonensis
Interleukin-10
Interleukin-13
description The initial encounter of Leishmania with its host’s immune system is important in the outcome of infection. Previous studies have shown that PBMCs from healthy volunteers (HV) exposed to Leishmania differ in IFN-γ production. We have expanded such observations evaluating the profi le and kinetics of cytokines (IFN-γ, IL-12p70, IL-10, IL-13), chemokines (CCL5, CCL3, CCL4, CXCL10), and chemokine receptors (CCR1,CCR5, CXCR3, CCR4) in vitro L. amazonensis-stimulated of HV´s PBMCs. HVs were divided in groups of high (HR) or low (LR) IFN-γ responders. In both groups, HR and LR, after L. amazonensis infection there was a predominance of IL-10 and IL-13 over IFN-γ production, while IL-12 was produced in similar amount. Regarding chemokines, a more striking difference was observed for CCL3 expression that was lower at 12 hours and 48 hours post infection in LR than in HR. Interestingly, a downregulation of CCR5 and a greater expression of CCR4 were found in low IFN-γ responders. These data suggest that early after L. amazonensis infection there is a cytokine milieu dominated by IL-13 and IL-10, and despite of this environment, IFN-γ is produced, supporting the complexity of the response. It is noteworthy that the pattern of immune response is mounted in fi rst hours after Leishmania stimulation, with the defi nition of the differentiation of Th1 versus Th2 cells. It remains to be determined if such an in vitro difference has an in vivo counterpart in terms of susceptibility to infection
publishDate 2010
dc.date.none.fl_str_mv 2010
2021-12-20T14:46:57Z
2021-12-20T14:46:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv COÊLHO, Zirlane Castelo B et al. In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13. The Brazilian Journal of Infectious Diseases, [s. l.], v. 14, p. 478-484, 2010.
http://www.repositorio.ufc.br/handle/riufc/63120
identifier_str_mv COÊLHO, Zirlane Castelo B et al. In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13. The Brazilian Journal of Infectious Diseases, [s. l.], v. 14, p. 478-484, 2010.
url http://www.repositorio.ufc.br/handle/riufc/63120
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Brazilian Journal of Infectious Diseases
publisher.none.fl_str_mv The Brazilian Journal of Infectious Diseases
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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