In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/63120 |
Resumo: | The initial encounter of Leishmania with its host’s immune system is important in the outcome of infection. Previous studies have shown that PBMCs from healthy volunteers (HV) exposed to Leishmania differ in IFN-γ production. We have expanded such observations evaluating the profi le and kinetics of cytokines (IFN-γ, IL-12p70, IL-10, IL-13), chemokines (CCL5, CCL3, CCL4, CXCL10), and chemokine receptors (CCR1,CCR5, CXCR3, CCR4) in vitro L. amazonensis-stimulated of HV´s PBMCs. HVs were divided in groups of high (HR) or low (LR) IFN-γ responders. In both groups, HR and LR, after L. amazonensis infection there was a predominance of IL-10 and IL-13 over IFN-γ production, while IL-12 was produced in similar amount. Regarding chemokines, a more striking difference was observed for CCL3 expression that was lower at 12 hours and 48 hours post infection in LR than in HR. Interestingly, a downregulation of CCR5 and a greater expression of CCR4 were found in low IFN-γ responders. These data suggest that early after L. amazonensis infection there is a cytokine milieu dominated by IL-13 and IL-10, and despite of this environment, IFN-γ is produced, supporting the complexity of the response. It is noteworthy that the pattern of immune response is mounted in fi rst hours after Leishmania stimulation, with the defi nition of the differentiation of Th1 versus Th2 cells. It remains to be determined if such an in vitro difference has an in vivo counterpart in terms of susceptibility to infection |
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In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13Leishmania amazonensisInterleukin-10Interleukin-13The initial encounter of Leishmania with its host’s immune system is important in the outcome of infection. Previous studies have shown that PBMCs from healthy volunteers (HV) exposed to Leishmania differ in IFN-γ production. We have expanded such observations evaluating the profi le and kinetics of cytokines (IFN-γ, IL-12p70, IL-10, IL-13), chemokines (CCL5, CCL3, CCL4, CXCL10), and chemokine receptors (CCR1,CCR5, CXCR3, CCR4) in vitro L. amazonensis-stimulated of HV´s PBMCs. HVs were divided in groups of high (HR) or low (LR) IFN-γ responders. In both groups, HR and LR, after L. amazonensis infection there was a predominance of IL-10 and IL-13 over IFN-γ production, while IL-12 was produced in similar amount. Regarding chemokines, a more striking difference was observed for CCL3 expression that was lower at 12 hours and 48 hours post infection in LR than in HR. Interestingly, a downregulation of CCR5 and a greater expression of CCR4 were found in low IFN-γ responders. These data suggest that early after L. amazonensis infection there is a cytokine milieu dominated by IL-13 and IL-10, and despite of this environment, IFN-γ is produced, supporting the complexity of the response. It is noteworthy that the pattern of immune response is mounted in fi rst hours after Leishmania stimulation, with the defi nition of the differentiation of Th1 versus Th2 cells. It remains to be determined if such an in vitro difference has an in vivo counterpart in terms of susceptibility to infectionThe Brazilian Journal of Infectious Diseases2021-12-20T14:46:57Z2021-12-20T14:46:57Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCOÊLHO, Zirlane Castelo B et al. In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13. The Brazilian Journal of Infectious Diseases, [s. l.], v. 14, p. 478-484, 2010.http://www.repositorio.ufc.br/handle/riufc/63120Coêlho, Zirlane Castelo BTeixeira, Maria JaniaMota, Erika FreitasFrutuoso, Mércia SindeauxSilva, João Santana daBarral, AldinaBarral Netto, ManoelPompeu, Margarida Maria Linfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFC2023-10-10T19:38:21Zoai:repositorio.ufc.br:riufc/63120Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:45:25.153093Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13 |
title |
In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13 |
spellingShingle |
In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13 Coêlho, Zirlane Castelo B Leishmania amazonensis Interleukin-10 Interleukin-13 |
title_short |
In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13 |
title_full |
In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13 |
title_fullStr |
In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13 |
title_full_unstemmed |
In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13 |
title_sort |
In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13 |
author |
Coêlho, Zirlane Castelo B |
author_facet |
Coêlho, Zirlane Castelo B Teixeira, Maria Jania Mota, Erika Freitas Frutuoso, Mércia Sindeaux Silva, João Santana da Barral, Aldina Barral Netto, Manoel Pompeu, Margarida Maria L |
author_role |
author |
author2 |
Teixeira, Maria Jania Mota, Erika Freitas Frutuoso, Mércia Sindeaux Silva, João Santana da Barral, Aldina Barral Netto, Manoel Pompeu, Margarida Maria L |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Coêlho, Zirlane Castelo B Teixeira, Maria Jania Mota, Erika Freitas Frutuoso, Mércia Sindeaux Silva, João Santana da Barral, Aldina Barral Netto, Manoel Pompeu, Margarida Maria L |
dc.subject.por.fl_str_mv |
Leishmania amazonensis Interleukin-10 Interleukin-13 |
topic |
Leishmania amazonensis Interleukin-10 Interleukin-13 |
description |
The initial encounter of Leishmania with its host’s immune system is important in the outcome of infection. Previous studies have shown that PBMCs from healthy volunteers (HV) exposed to Leishmania differ in IFN-γ production. We have expanded such observations evaluating the profi le and kinetics of cytokines (IFN-γ, IL-12p70, IL-10, IL-13), chemokines (CCL5, CCL3, CCL4, CXCL10), and chemokine receptors (CCR1,CCR5, CXCR3, CCR4) in vitro L. amazonensis-stimulated of HV´s PBMCs. HVs were divided in groups of high (HR) or low (LR) IFN-γ responders. In both groups, HR and LR, after L. amazonensis infection there was a predominance of IL-10 and IL-13 over IFN-γ production, while IL-12 was produced in similar amount. Regarding chemokines, a more striking difference was observed for CCL3 expression that was lower at 12 hours and 48 hours post infection in LR than in HR. Interestingly, a downregulation of CCR5 and a greater expression of CCR4 were found in low IFN-γ responders. These data suggest that early after L. amazonensis infection there is a cytokine milieu dominated by IL-13 and IL-10, and despite of this environment, IFN-γ is produced, supporting the complexity of the response. It is noteworthy that the pattern of immune response is mounted in fi rst hours after Leishmania stimulation, with the defi nition of the differentiation of Th1 versus Th2 cells. It remains to be determined if such an in vitro difference has an in vivo counterpart in terms of susceptibility to infection |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2021-12-20T14:46:57Z 2021-12-20T14:46:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
COÊLHO, Zirlane Castelo B et al. In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13. The Brazilian Journal of Infectious Diseases, [s. l.], v. 14, p. 478-484, 2010. http://www.repositorio.ufc.br/handle/riufc/63120 |
identifier_str_mv |
COÊLHO, Zirlane Castelo B et al. In vitro initial immune response against Leishmania amazonensis infection is characterized by an increased production of IL-10 and IL-13. The Brazilian Journal of Infectious Diseases, [s. l.], v. 14, p. 478-484, 2010. |
url |
http://www.repositorio.ufc.br/handle/riufc/63120 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
The Brazilian Journal of Infectious Diseases |
publisher.none.fl_str_mv |
The Brazilian Journal of Infectious Diseases |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
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1813028933994545152 |