Desenvolvimento, eficácia clínica e microbiológica de dentifrício incorporado com própolis vermelha brasileira

• Autor(a) principal: Furtado Júnior, João Hildo de Carvalho
• Data de Publicação: 2019
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/40449
• topic: Própolis; Biofilme; Produtos naturais; Saliva

Introduction: Propolis is a resinous complex with several biological activities documented in the literature, several are products derived from propolis and every day patent deposits are made. The buccal cavity is quite complex, and in individuals with retentive factors, such as orthodontic patients, a greater accumulation of the dental biofilm occurs. Objectives: To carry out a prospective study of dental patents on propolis. To develop, evaluate in vitro and clinical the efficacy of a dentifrice based on Brazilian red propolis extract (PVB). Material and Methods: This is a three-stage study. In the first stage a prospective patent study on propolis and dentistry in intellectual property banks was carried out. In the second step, the antimicrobial activity of the extract and dentrifricium of PVB was verified in vitro, and compared to the dentifrice Parodontax. Strains of Streptococcus mutans (S.mutans) were used and the microdilution method in culture broth was chosen. The third stage was a clinical trial, in which a total of 92 caries-free participants were randomized into two groups: Group I - Common fluoridated dentifrice and Group II - Dentifrice PVB. Each group used the toothpaste for a period of 28 days, three times daily. Gingival examination was performed to evaluate the marginal bleeding index (ISM) and saliva was collected at the baseline (T0) and after 28 days (T28) for counts of colony forming units (CFU) of gram-negative bacteria and S.mutans. The ISM and UFC (log10) data were expressed as mean and standard deviation, submitted to the Kolmogorov-Smirnov normality test and compared using the Wilcoxon and Mann-Whitney tests. Results: There were found 62 products of propolis deposited in intellectual property banks in the world. In the in vitro study the propolis extract and dentifrice showed efficacy against S.mutans up to the concentration of 0.3906 μg/mL and the Parodontax dentifrice showed antimicrobial activity at all dilutions. There was thus no difference between the propolis extract and the dentifrice incorporated with the extract. In the clinical phase, the two groups had statistically significant ISM reduction, and there was no difference in the intergroup analysis. During the treatment period the PVB dentifrice showed better clinical and antimicrobial activity. Future studies are needed to identify better effects to establish the use of dentifrice in the control of dental biofilm.