Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/63486 |
Resumo: | The germin-like protein (GLP) purified from Thevetia peruviana, Peruvianin-I, is the only one described as having proteolytic activity. Therefore, the goal of this study was to investigate the structural features responsible for its enzymatic activity. Although the amino acid sequence of Peruvianin-I showed high identity with other GLPs, it exhibited punctual mutations, which were responsible for the absence of oxalate oxidase activity. The phylogenetic analysis showed that Peruvianin-I does not belong to any classification of GLP subfamilies. Moreover, Peruvianin-I contains a catalytic triad found in all plant cysteine peptidases. Molecular docking simulations confirmed the role of the catalytic triad in its proteolytic activity. Synchrotron radiation circular dichroism assays confirmed that Peruvianin-I was stable at pH ranging from 5.0 to 8.0 and that it presented significant structural changes only above 60 °C. The addition of iodoacetamide caused changes in its native conformation, but only a slight effect was observed after adding a reducing agent. This study reports an unusual protein with germin-like structure, lacking typical oxalate oxidase activity. Instead, the proteolytic activity observed suggests that the protein is a cysteine peptidase. These structural peculiarities make Peruvianin‑I an interesting model for further understanding of the action of laticifer fluids in plant defense. |
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Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activityGLPLaticiferOxalate oxidaseThe germin-like protein (GLP) purified from Thevetia peruviana, Peruvianin-I, is the only one described as having proteolytic activity. Therefore, the goal of this study was to investigate the structural features responsible for its enzymatic activity. Although the amino acid sequence of Peruvianin-I showed high identity with other GLPs, it exhibited punctual mutations, which were responsible for the absence of oxalate oxidase activity. The phylogenetic analysis showed that Peruvianin-I does not belong to any classification of GLP subfamilies. Moreover, Peruvianin-I contains a catalytic triad found in all plant cysteine peptidases. Molecular docking simulations confirmed the role of the catalytic triad in its proteolytic activity. Synchrotron radiation circular dichroism assays confirmed that Peruvianin-I was stable at pH ranging from 5.0 to 8.0 and that it presented significant structural changes only above 60 °C. The addition of iodoacetamide caused changes in its native conformation, but only a slight effect was observed after adding a reducing agent. This study reports an unusual protein with germin-like structure, lacking typical oxalate oxidase activity. Instead, the proteolytic activity observed suggests that the protein is a cysteine peptidase. These structural peculiarities make Peruvianin‑I an interesting model for further understanding of the action of laticifer fluids in plant defense.International Journal of Biological Macromolecules2022-01-13T17:23:19Z2022-01-13T17:23:19Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCRUZ, Wallace T. da et al. Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity. International Journal of Biological Macromolecules, [s. l.], v. 126, p. 1167-1176, 2019.http://www.repositorio.ufc.br/handle/riufc/63486Cruz, Wallace T. daBezerra, Eduardo H. S.Grangeiro, Thalles B.Lopes, Jose L. S.Silva, Maria Z. R.Ramos, Márcio VianaRocha, Bruno A. M.Oliveira, Jefferson S.Freitas, Deborah C.Freitas, Cleverson D. T.info:eu-repo/semantics/openAccessporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFC2023-10-10T19:33:28Zoai:repositorio.ufc.br:riufc/63486Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:26:40.354945Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity |
title |
Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity |
spellingShingle |
Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity Cruz, Wallace T. da GLP Laticifer Oxalate oxidase |
title_short |
Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity |
title_full |
Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity |
title_fullStr |
Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity |
title_full_unstemmed |
Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity |
title_sort |
Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity |
author |
Cruz, Wallace T. da |
author_facet |
Cruz, Wallace T. da Bezerra, Eduardo H. S. Grangeiro, Thalles B. Lopes, Jose L. S. Silva, Maria Z. R. Ramos, Márcio Viana Rocha, Bruno A. M. Oliveira, Jefferson S. Freitas, Deborah C. Freitas, Cleverson D. T. |
author_role |
author |
author2 |
Bezerra, Eduardo H. S. Grangeiro, Thalles B. Lopes, Jose L. S. Silva, Maria Z. R. Ramos, Márcio Viana Rocha, Bruno A. M. Oliveira, Jefferson S. Freitas, Deborah C. Freitas, Cleverson D. T. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cruz, Wallace T. da Bezerra, Eduardo H. S. Grangeiro, Thalles B. Lopes, Jose L. S. Silva, Maria Z. R. Ramos, Márcio Viana Rocha, Bruno A. M. Oliveira, Jefferson S. Freitas, Deborah C. Freitas, Cleverson D. T. |
dc.subject.por.fl_str_mv |
GLP Laticifer Oxalate oxidase |
topic |
GLP Laticifer Oxalate oxidase |
description |
The germin-like protein (GLP) purified from Thevetia peruviana, Peruvianin-I, is the only one described as having proteolytic activity. Therefore, the goal of this study was to investigate the structural features responsible for its enzymatic activity. Although the amino acid sequence of Peruvianin-I showed high identity with other GLPs, it exhibited punctual mutations, which were responsible for the absence of oxalate oxidase activity. The phylogenetic analysis showed that Peruvianin-I does not belong to any classification of GLP subfamilies. Moreover, Peruvianin-I contains a catalytic triad found in all plant cysteine peptidases. Molecular docking simulations confirmed the role of the catalytic triad in its proteolytic activity. Synchrotron radiation circular dichroism assays confirmed that Peruvianin-I was stable at pH ranging from 5.0 to 8.0 and that it presented significant structural changes only above 60 °C. The addition of iodoacetamide caused changes in its native conformation, but only a slight effect was observed after adding a reducing agent. This study reports an unusual protein with germin-like structure, lacking typical oxalate oxidase activity. Instead, the proteolytic activity observed suggests that the protein is a cysteine peptidase. These structural peculiarities make Peruvianin‑I an interesting model for further understanding of the action of laticifer fluids in plant defense. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2022-01-13T17:23:19Z 2022-01-13T17:23:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CRUZ, Wallace T. da et al. Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity. International Journal of Biological Macromolecules, [s. l.], v. 126, p. 1167-1176, 2019. http://www.repositorio.ufc.br/handle/riufc/63486 |
identifier_str_mv |
CRUZ, Wallace T. da et al. Structural and enzymatic characterization of Peruvianin‑I, the first germin-like protein with proteolytic activity. International Journal of Biological Macromolecules, [s. l.], v. 126, p. 1167-1176, 2019. |
url |
http://www.repositorio.ufc.br/handle/riufc/63486 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
International Journal of Biological Macromolecules |
publisher.none.fl_str_mv |
International Journal of Biological Macromolecules |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1813028806100779008 |