Efeito de um biopolímero, goma do cajueiro, na colite induzida por ácido trinitrobenzenossulfônico em ratos

• Autor(a) principal: Marques, Adryano Gonçalves
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/42425
• topic: Anacardium occidentale; Inflamação; Colite; Gomas Vegetais

Inflammatory bowel diseases (IBDs) encompass a group of diseases characterized by dysregulation of the immune response of the digestive tract to normal intestinal flora in susceptible individuals, leading to a chronic and recurrent inflammatory process. Its main representatives are Crohn's disease (CD) and ulcerative colitis (UC). Currently the treatment of IBDs is defined in an individualized way, however the drugs currently used are still associated with relevant side effects and a high economic cost. The objective of this study was to evaluate the effect of cashew gum (CG), a natural biopolymer obtained from the bark of the Brazilian Northeast (Anacardium occidentale L.) on trinitrobenzenesulfonic acid (TNBS) induced colitis in rats. The experimental study was performed in two stages. Initially, the impact of CG at 10% on colonic mucosal barrier of rats was evaluated 7 days after induction of colitis by TNBS or after application of saline solution (0.9%). For this purpose, colonic samples from rats were mounted in Üssing chambers to evaluate the permeability to fluorescein. In the second stage, the anti-inflammatory effect of the CG 10% was tested, applying it as a rectal enema at doses of 10 mg/Kg and 30 mg/Kg for 7 days in animals with TNBS-induced colitis. A barrier effect was observed after application of CG 10%, evidenced by a significant decrease (p: 0.04) in permeability at minute 90 in animals on the 7th day after induction of colitis (33.84 ± 9,576 RFI), when compared to baseline permeability (92.87 ± 29.60 RFI). All the inflammatory parameters evaluated (intra-abdominal adhesion score, wet weight evaluation, macroscopic and microscopic inflammatory scores, and myeloperoxidase dosage) remained altered after 7 days of treatment with a CG 10% enema, indicating no significant anti-inflammatory action in both doses. From this result we can conclude that cashew gum has an in vitro barrier effect in animals with TNBS induced colitis similar to that exerted by the intestinal mucosal barrier. However, there was no in vivo anti-inflammatory action in the same model of experimental colitis.