Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats

Detalhes bibliográficos
Autor(a) principal: Acioli, Porfírio Cezar Passos
Data de Publicação: 2014
Outros Autores: Albuquerque, Andrea de Oliveira, Guimarães, Isaac Bevilaqua de Albuquerque, Araujo, Roberto Wagner Bezerra de, Vasconcelos, Paulo Roberto Leitão, Guimarães, Sérgio Botelho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/9473
Resumo: ABSTRACTPURPOSE: To evaluate the effects of acupuncture (Ac) and electroacupuncture (EAc) on oxidative stress and inflammation in testis torsion/detorsion (T/D) model in rats.METHODS: Thirty male Wistar rats were randomized into five groups. G1 Group (Sham) served as control. The remaining groups were submitted to spermatic cord torsion (720°) for 3 hours, followed by detorsion and reperfusion for 4 hours. Before detorsion G3, G4 and G5 rats were treated with Ac, EAc 2Hz and EAc 10 Hz, respectively, applied to acupoint Gulai (S-29) bilaterally under anesthesia for 5 minutes. Next, the testes were detorsioned and reperfused for 4 hours. Afterwards, blood samples and the right testis were collected for biochemical assays: reduced Glutathione (GSH), Malonaldehyde (MDA), Myeloperoxidase (MPO).RESULTS: EAc stimulation (2 and 10 Hz) promoted significant increase in concentrations of GSH in plasma and testis of G4-G5 rats, compared with G1. There was significant increase of tissue MDA in groups G4-G5 and plasma MDA in all groups, compared with G1. There was a significant reduction in MPO activity in groups G4-G5 compared with G1.CONCLUSION: Electroacupuncture stimulation (2 and 10 Hz) attenuates oxidative stress and inflammatory response in rats subjected to testicular torsion/detorsion.
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spelling Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in ratsTorção do Cordão EspermáticoIsquemiaEletroacupunturaABSTRACTPURPOSE: To evaluate the effects of acupuncture (Ac) and electroacupuncture (EAc) on oxidative stress and inflammation in testis torsion/detorsion (T/D) model in rats.METHODS: Thirty male Wistar rats were randomized into five groups. G1 Group (Sham) served as control. The remaining groups were submitted to spermatic cord torsion (720°) for 3 hours, followed by detorsion and reperfusion for 4 hours. Before detorsion G3, G4 and G5 rats were treated with Ac, EAc 2Hz and EAc 10 Hz, respectively, applied to acupoint Gulai (S-29) bilaterally under anesthesia for 5 minutes. Next, the testes were detorsioned and reperfused for 4 hours. Afterwards, blood samples and the right testis were collected for biochemical assays: reduced Glutathione (GSH), Malonaldehyde (MDA), Myeloperoxidase (MPO).RESULTS: EAc stimulation (2 and 10 Hz) promoted significant increase in concentrations of GSH in plasma and testis of G4-G5 rats, compared with G1. There was significant increase of tissue MDA in groups G4-G5 and plasma MDA in all groups, compared with G1. There was a significant reduction in MPO activity in groups G4-G5 compared with G1.CONCLUSION: Electroacupuncture stimulation (2 and 10 Hz) attenuates oxidative stress and inflammatory response in rats subjected to testicular torsion/detorsion.Acta Cirúrgica Brasileira2014-10-16T16:45:55Z2014-10-16T16:45:55Z2014-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfACIOLI, P. C. P. et al. Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats. Acta Cirúrgica Brasileira, São Paulo, v. 29, n. 7, p. 450-456, jul., 2014.1678-2674 On linehttp://www.repositorio.ufc.br/handle/riufc/9473Acioli, Porfírio Cezar PassosAlbuquerque, Andrea de OliveiraGuimarães, Isaac Bevilaqua de AlbuquerqueAraujo, Roberto Wagner Bezerra deVasconcelos, Paulo Roberto LeitãoGuimarães, Sérgio Botelhoengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2018-12-17T13:31:12Zoai:repositorio.ufc.br:riufc/9473Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:17:01.550849Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats
title Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats
spellingShingle Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats
Acioli, Porfírio Cezar Passos
Torção do Cordão Espermático
Isquemia
Eletroacupuntura
title_short Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats
title_full Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats
title_fullStr Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats
title_full_unstemmed Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats
title_sort Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats
author Acioli, Porfírio Cezar Passos
author_facet Acioli, Porfírio Cezar Passos
Albuquerque, Andrea de Oliveira
Guimarães, Isaac Bevilaqua de Albuquerque
Araujo, Roberto Wagner Bezerra de
Vasconcelos, Paulo Roberto Leitão
Guimarães, Sérgio Botelho
author_role author
author2 Albuquerque, Andrea de Oliveira
Guimarães, Isaac Bevilaqua de Albuquerque
Araujo, Roberto Wagner Bezerra de
Vasconcelos, Paulo Roberto Leitão
Guimarães, Sérgio Botelho
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Acioli, Porfírio Cezar Passos
Albuquerque, Andrea de Oliveira
Guimarães, Isaac Bevilaqua de Albuquerque
Araujo, Roberto Wagner Bezerra de
Vasconcelos, Paulo Roberto Leitão
Guimarães, Sérgio Botelho
dc.subject.por.fl_str_mv Torção do Cordão Espermático
Isquemia
Eletroacupuntura
topic Torção do Cordão Espermático
Isquemia
Eletroacupuntura
description ABSTRACTPURPOSE: To evaluate the effects of acupuncture (Ac) and electroacupuncture (EAc) on oxidative stress and inflammation in testis torsion/detorsion (T/D) model in rats.METHODS: Thirty male Wistar rats were randomized into five groups. G1 Group (Sham) served as control. The remaining groups were submitted to spermatic cord torsion (720°) for 3 hours, followed by detorsion and reperfusion for 4 hours. Before detorsion G3, G4 and G5 rats were treated with Ac, EAc 2Hz and EAc 10 Hz, respectively, applied to acupoint Gulai (S-29) bilaterally under anesthesia for 5 minutes. Next, the testes were detorsioned and reperfused for 4 hours. Afterwards, blood samples and the right testis were collected for biochemical assays: reduced Glutathione (GSH), Malonaldehyde (MDA), Myeloperoxidase (MPO).RESULTS: EAc stimulation (2 and 10 Hz) promoted significant increase in concentrations of GSH in plasma and testis of G4-G5 rats, compared with G1. There was significant increase of tissue MDA in groups G4-G5 and plasma MDA in all groups, compared with G1. There was a significant reduction in MPO activity in groups G4-G5 compared with G1.CONCLUSION: Electroacupuncture stimulation (2 and 10 Hz) attenuates oxidative stress and inflammatory response in rats subjected to testicular torsion/detorsion.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-16T16:45:55Z
2014-10-16T16:45:55Z
2014-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ACIOLI, P. C. P. et al. Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats. Acta Cirúrgica Brasileira, São Paulo, v. 29, n. 7, p. 450-456, jul., 2014.
1678-2674 On line
http://www.repositorio.ufc.br/handle/riufc/9473
identifier_str_mv ACIOLI, P. C. P. et al. Protective effects of abdominal electroacupuncture on oxidative stress and inflammation due to testis torsion/detorsion in rats. Acta Cirúrgica Brasileira, São Paulo, v. 29, n. 7, p. 450-456, jul., 2014.
1678-2674 On line
url http://www.repositorio.ufc.br/handle/riufc/9473
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Acta Cirúrgica Brasileira
publisher.none.fl_str_mv Acta Cirúrgica Brasileira
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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