Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia

Detalhes bibliográficos
Autor(a) principal: Lima, José Vinícius Leite
Data de Publicação: 2017
Outros Autores: Martins, Suzana Cláudia Silveira, Siqueira, Katia Aparecida de, Soares, Marcos Antônio, Martins, Claudia Miranda
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/64241
Resumo: The actinobacteria are the most abundant bacterial group in the soil, where they present different characteristics and antagonistic effects on other microorganisms. The objectives of this study were to characterize and evaluate the antagonistic effect of actinobacteria from the semiarid region on rhizobia from the same climatic region. Tests were performed to observe chromogenic and micro-morphological characteristics, tolerance to pH levels of melanin production, and use of carbon sources. This occurred due to the use of modified CDA culture media and basal medium supplemented with specific carbon sources, as well as the use of Ral color chat to observe the colonies. The in vitro antagonism on rhizobia was evaluated in yeast malt agar (YMA) culture medium for the observation of inhibition halo by actinobacteria. We observed different characteristics in relation to the color of aerial mycelium and reverse pigments; tolerance to media with acid and alkaline pH; and production of melanoid pigment occurred only in three strains. The morphological characteristics were described for genera Streptomyces and Saccharothrix, later confirmed by molecular sequencing. These genera were generalists in use of carbon sources, showing their physiological versatility. Regarding antagonism, nine strains of the genus Streptomyces were able to inhibit in vitro strains of Rhizobium tropici and Bradyrhizobium yuanmingense also coming from semiarid soils. The antagonism between actinobacteria and rhizobia can directly affect the symbiosis between nitrogen-fixing bacteria and leguminous plants. These results are pioneering in observing antagonism of these species of rhizobia under the climate condition of the semiarid region, and may contribute to agricultural biotechnology.
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spelling Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobiaStreptomycesSoilMicrobe interactionsBrazilAgricultural biotechnologyActinobacteriaAntagonistic effectThe actinobacteria are the most abundant bacterial group in the soil, where they present different characteristics and antagonistic effects on other microorganisms. The objectives of this study were to characterize and evaluate the antagonistic effect of actinobacteria from the semiarid region on rhizobia from the same climatic region. Tests were performed to observe chromogenic and micro-morphological characteristics, tolerance to pH levels of melanin production, and use of carbon sources. This occurred due to the use of modified CDA culture media and basal medium supplemented with specific carbon sources, as well as the use of Ral color chat to observe the colonies. The in vitro antagonism on rhizobia was evaluated in yeast malt agar (YMA) culture medium for the observation of inhibition halo by actinobacteria. We observed different characteristics in relation to the color of aerial mycelium and reverse pigments; tolerance to media with acid and alkaline pH; and production of melanoid pigment occurred only in three strains. The morphological characteristics were described for genera Streptomyces and Saccharothrix, later confirmed by molecular sequencing. These genera were generalists in use of carbon sources, showing their physiological versatility. Regarding antagonism, nine strains of the genus Streptomyces were able to inhibit in vitro strains of Rhizobium tropici and Bradyrhizobium yuanmingense also coming from semiarid soils. The antagonism between actinobacteria and rhizobia can directly affect the symbiosis between nitrogen-fixing bacteria and leguminous plants. These results are pioneering in observing antagonism of these species of rhizobia under the climate condition of the semiarid region, and may contribute to agricultural biotechnology.African Journal of Biotechnology2022-03-03T15:41:55Z2022-03-03T15:41:55Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLIMA, José Vinícius Leite et al. Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia. African Journal of Biotechnology, [s. l.], v. 16, n. 11, p. 499-507, 2017http://www.repositorio.ufc.br/handle/riufc/64241Lima, José Vinícius LeiteMartins, Suzana Cláudia SilveiraSiqueira, Katia Aparecida deSoares, Marcos AntônioMartins, Claudia Mirandainfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFC2023-10-10T19:36:38Zoai:repositorio.ufc.br:riufc/64241Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:38:40.348763Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia
title Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia
spellingShingle Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia
Lima, José Vinícius Leite
Streptomyces
Soil
Microbe interactions
Brazil
Agricultural biotechnology
Actinobacteria
Antagonistic effect
title_short Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia
title_full Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia
title_fullStr Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia
title_full_unstemmed Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia
title_sort Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia
author Lima, José Vinícius Leite
author_facet Lima, José Vinícius Leite
Martins, Suzana Cláudia Silveira
Siqueira, Katia Aparecida de
Soares, Marcos Antônio
Martins, Claudia Miranda
author_role author
author2 Martins, Suzana Cláudia Silveira
Siqueira, Katia Aparecida de
Soares, Marcos Antônio
Martins, Claudia Miranda
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lima, José Vinícius Leite
Martins, Suzana Cláudia Silveira
Siqueira, Katia Aparecida de
Soares, Marcos Antônio
Martins, Claudia Miranda
dc.subject.por.fl_str_mv Streptomyces
Soil
Microbe interactions
Brazil
Agricultural biotechnology
Actinobacteria
Antagonistic effect
topic Streptomyces
Soil
Microbe interactions
Brazil
Agricultural biotechnology
Actinobacteria
Antagonistic effect
description The actinobacteria are the most abundant bacterial group in the soil, where they present different characteristics and antagonistic effects on other microorganisms. The objectives of this study were to characterize and evaluate the antagonistic effect of actinobacteria from the semiarid region on rhizobia from the same climatic region. Tests were performed to observe chromogenic and micro-morphological characteristics, tolerance to pH levels of melanin production, and use of carbon sources. This occurred due to the use of modified CDA culture media and basal medium supplemented with specific carbon sources, as well as the use of Ral color chat to observe the colonies. The in vitro antagonism on rhizobia was evaluated in yeast malt agar (YMA) culture medium for the observation of inhibition halo by actinobacteria. We observed different characteristics in relation to the color of aerial mycelium and reverse pigments; tolerance to media with acid and alkaline pH; and production of melanoid pigment occurred only in three strains. The morphological characteristics were described for genera Streptomyces and Saccharothrix, later confirmed by molecular sequencing. These genera were generalists in use of carbon sources, showing their physiological versatility. Regarding antagonism, nine strains of the genus Streptomyces were able to inhibit in vitro strains of Rhizobium tropici and Bradyrhizobium yuanmingense also coming from semiarid soils. The antagonism between actinobacteria and rhizobia can directly affect the symbiosis between nitrogen-fixing bacteria and leguminous plants. These results are pioneering in observing antagonism of these species of rhizobia under the climate condition of the semiarid region, and may contribute to agricultural biotechnology.
publishDate 2017
dc.date.none.fl_str_mv 2017
2022-03-03T15:41:55Z
2022-03-03T15:41:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv LIMA, José Vinícius Leite et al. Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia. African Journal of Biotechnology, [s. l.], v. 16, n. 11, p. 499-507, 2017
http://www.repositorio.ufc.br/handle/riufc/64241
identifier_str_mv LIMA, José Vinícius Leite et al. Characterization of actinobacteria from the semiarid region, and their antagonistic effect on strains of rhizobia. African Journal of Biotechnology, [s. l.], v. 16, n. 11, p. 499-507, 2017
url http://www.repositorio.ufc.br/handle/riufc/64241
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv African Journal of Biotechnology
publisher.none.fl_str_mv African Journal of Biotechnology
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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