New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads

Detalhes bibliográficos
Autor(a) principal: Barreto, R.V.G.
Data de Publicação: 2010
Outros Autores: Hissa, Denise Cavalcante, Paes, Fernanda Araújo, Grangeiro, T. B., Nascimento, R. F., Rebelo, Luciana Magalhães, Craveiro, A. A., Melo, V. M. M.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/62956
Resumo: Spores ofBacillus subtilisLAMI008 were entrapped in 3-mm chitosan beads and cross-linked with 0.3%glutaraldehyde forn-hexadecane biodegradation and biosurfactant recovery. When exposed to nutrients,the spores generated vegetative cells without morphological alterations as revealed by atomic forcemicroscopy. The entrapped cells degraded almost 100% of 1% ofn-hexadecane in medium supplementedwith 1% glucose and produce biosurfactant within 48 h, as well as free cells. The number of viable cellsinside the beads was maintained throughout then-hexadecane degradation process and the released bio-surfactant was not used as a carbon source. Entrapment of bacterial spores in chitosan beads overcomesproblems with stability, storage, and long term cell viability encountered with vegetative cells. Thisapproach can potentially be utilized for biodegradation of complex compounds by entrapping sporesof different species of bacteria.
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spelling New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beadsImmobilizationSporesChitosanHexadecaneBiodegradationSpores ofBacillus subtilisLAMI008 were entrapped in 3-mm chitosan beads and cross-linked with 0.3%glutaraldehyde forn-hexadecane biodegradation and biosurfactant recovery. When exposed to nutrients,the spores generated vegetative cells without morphological alterations as revealed by atomic forcemicroscopy. The entrapped cells degraded almost 100% of 1% ofn-hexadecane in medium supplementedwith 1% glucose and produce biosurfactant within 48 h, as well as free cells. The number of viable cellsinside the beads was maintained throughout then-hexadecane degradation process and the released bio-surfactant was not used as a carbon source. Entrapment of bacterial spores in chitosan beads overcomesproblems with stability, storage, and long term cell viability encountered with vegetative cells. Thisapproach can potentially be utilized for biodegradation of complex compounds by entrapping sporesof different species of bacteria.Bioresource Technology2021-12-13T17:44:01Z2021-12-13T17:44:01Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBARRETO, R.V.G. et al. New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads. Bioresource Technolog, [s. l.], v. 101, p. 2121-2125, 2010http://www.repositorio.ufc.br/handle/riufc/62956Barreto, R.V.G.Hissa, Denise CavalcantePaes, Fernanda AraújoGrangeiro, T. B.Nascimento, R. F.Rebelo, Luciana MagalhãesCraveiro, A. A.Melo, V. M. M.info:eu-repo/semantics/openAccessporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFC2023-10-10T19:35:57Zoai:repositorio.ufc.br:riufc/62956Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:34:51.667981Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads
title New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads
spellingShingle New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads
Barreto, R.V.G.
Immobilization
Spores
Chitosan
Hexadecane
Biodegradation
title_short New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads
title_full New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads
title_fullStr New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads
title_full_unstemmed New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads
title_sort New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads
author Barreto, R.V.G.
author_facet Barreto, R.V.G.
Hissa, Denise Cavalcante
Paes, Fernanda Araújo
Grangeiro, T. B.
Nascimento, R. F.
Rebelo, Luciana Magalhães
Craveiro, A. A.
Melo, V. M. M.
author_role author
author2 Hissa, Denise Cavalcante
Paes, Fernanda Araújo
Grangeiro, T. B.
Nascimento, R. F.
Rebelo, Luciana Magalhães
Craveiro, A. A.
Melo, V. M. M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barreto, R.V.G.
Hissa, Denise Cavalcante
Paes, Fernanda Araújo
Grangeiro, T. B.
Nascimento, R. F.
Rebelo, Luciana Magalhães
Craveiro, A. A.
Melo, V. M. M.
dc.subject.por.fl_str_mv Immobilization
Spores
Chitosan
Hexadecane
Biodegradation
topic Immobilization
Spores
Chitosan
Hexadecane
Biodegradation
description Spores ofBacillus subtilisLAMI008 were entrapped in 3-mm chitosan beads and cross-linked with 0.3%glutaraldehyde forn-hexadecane biodegradation and biosurfactant recovery. When exposed to nutrients,the spores generated vegetative cells without morphological alterations as revealed by atomic forcemicroscopy. The entrapped cells degraded almost 100% of 1% ofn-hexadecane in medium supplementedwith 1% glucose and produce biosurfactant within 48 h, as well as free cells. The number of viable cellsinside the beads was maintained throughout then-hexadecane degradation process and the released bio-surfactant was not used as a carbon source. Entrapment of bacterial spores in chitosan beads overcomesproblems with stability, storage, and long term cell viability encountered with vegetative cells. Thisapproach can potentially be utilized for biodegradation of complex compounds by entrapping sporesof different species of bacteria.
publishDate 2010
dc.date.none.fl_str_mv 2010
2021-12-13T17:44:01Z
2021-12-13T17:44:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BARRETO, R.V.G. et al. New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads. Bioresource Technolog, [s. l.], v. 101, p. 2121-2125, 2010
http://www.repositorio.ufc.br/handle/riufc/62956
identifier_str_mv BARRETO, R.V.G. et al. New approach for petroleum hydrocarbon degradation using bacterial spores entrapped in chitosan beads. Bioresource Technolog, [s. l.], v. 101, p. 2121-2125, 2010
url http://www.repositorio.ufc.br/handle/riufc/62956
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Bioresource Technology
publisher.none.fl_str_mv Bioresource Technology
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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