A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/4787 |
Resumo: | Studies in different populations have shown that vaccination with BCG provides partial protection against leprosy. However, need of boost BCG vaccination and your impact in response immune against antigens of Mycobacterium leprae is poorly understood, mainly in children who are contacts of these patients with leprosy, and constitute population of risk for illness. This study, we investigated the paper a second dose of BCG given to contacts of leprosy patients under the age of fifteen years in modulate the response imune. Blood samples of twenty five children, who received a first dose of BCG at birth, were collected immediately before and two months after BCG revaccination. Your peripheral blood mononuclear cells (PBMC) stimulated by proteins and peptides of M. leprae, were measured IFN-γ and IL-10 by an ELISA assay. Before revaccination the serum anti-PGL1 IgG and IgM isotypes were measured by ELISA assays performed with native PGL1- coated microplates, and PPD-skin reaction was measured 2 to 3 months after revaccination. The study was approved by the local Ethic Committee, number 006-08, May 7th, 2008. BCG revaccination can induces IFN-gamma and/or IL-10 production related to antigen and age, but did not correlated with PPD-skin reaction or anti-PGL1 levels. The PBMC’s production of IFN-gamma against MLT (total M. leprae antigen) after BCG was higher on children under 4 years old (N= 8, 4203,0 ± 539,2 pg/mL before BCG x 9141,0 ± 860,9 pg/mL after BCG, p=0,015, Mann-Whitney’s test). Children between 5 and 9 years old showed an increase either of IFN-gama levels (N=11, 4912 ± 1065 pg/mL x 9249,0 ± 1171 pg/mL, p=0,024, Mann-Whitney’s test) or IL-10 levels (N=11, 210,6 ± 39,4 pg/mL x 680,3 ± 76,74 pg/mL, p=0, 0,001, Mann-Whitney’s test). However children between 10 and 15 years old failed to increase the cytokines levels after BCG booster. Contacts of multibacillary patients produced higher IFN-gamma levels (N= 13, 4536,0 ± 543,1 pg/mL x 9263,0 ± 989,0 pg/mL, p=0,0012, Mann-Whitney’s test) and IL-10 levels (N= 13, 235,9 ± 46,5 pg/mL x 743,5 ± 87,8, p=0,0012, Mann-Whitney’s test) against MLT after BCG, but only IFN-gamma levels (N=12, 4975,0 ± 995,8 pg/mL x 8126,0 ± 788,6, p=0,0342, Mann-Whitney’s test) increased after BCG on contacts of paucibacillary patients. The production of IFN-gamma and IL-10 against two synthetic peptides (p38 and p67), before and after BCG vaccine, were similar as seen with MLT antigen, but the absence of cytokine production against p69 help to identify this peptide as a effective diagnostic marker. |
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A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníaseRevaccination with BCG modulates cytokine production against antigens of Mycobacterium leprae in contacts under 15 years of leprosy patientsHanseníaseMycobacterium bovisVacina BCGCitocinasStudies in different populations have shown that vaccination with BCG provides partial protection against leprosy. However, need of boost BCG vaccination and your impact in response immune against antigens of Mycobacterium leprae is poorly understood, mainly in children who are contacts of these patients with leprosy, and constitute population of risk for illness. This study, we investigated the paper a second dose of BCG given to contacts of leprosy patients under the age of fifteen years in modulate the response imune. Blood samples of twenty five children, who received a first dose of BCG at birth, were collected immediately before and two months after BCG revaccination. Your peripheral blood mononuclear cells (PBMC) stimulated by proteins and peptides of M. leprae, were measured IFN-γ and IL-10 by an ELISA assay. Before revaccination the serum anti-PGL1 IgG and IgM isotypes were measured by ELISA assays performed with native PGL1- coated microplates, and PPD-skin reaction was measured 2 to 3 months after revaccination. The study was approved by the local Ethic Committee, number 006-08, May 7th, 2008. BCG revaccination can induces IFN-gamma and/or IL-10 production related to antigen and age, but did not correlated with PPD-skin reaction or anti-PGL1 levels. The PBMC’s production of IFN-gamma against MLT (total M. leprae antigen) after BCG was higher on children under 4 years old (N= 8, 4203,0 ± 539,2 pg/mL before BCG x 9141,0 ± 860,9 pg/mL after BCG, p=0,015, Mann-Whitney’s test). Children between 5 and 9 years old showed an increase either of IFN-gama levels (N=11, 4912 ± 1065 pg/mL x 9249,0 ± 1171 pg/mL, p=0,024, Mann-Whitney’s test) or IL-10 levels (N=11, 210,6 ± 39,4 pg/mL x 680,3 ± 76,74 pg/mL, p=0, 0,001, Mann-Whitney’s test). However children between 10 and 15 years old failed to increase the cytokines levels after BCG booster. Contacts of multibacillary patients produced higher IFN-gamma levels (N= 13, 4536,0 ± 543,1 pg/mL x 9263,0 ± 989,0 pg/mL, p=0,0012, Mann-Whitney’s test) and IL-10 levels (N= 13, 235,9 ± 46,5 pg/mL x 743,5 ± 87,8, p=0,0012, Mann-Whitney’s test) against MLT after BCG, but only IFN-gamma levels (N=12, 4975,0 ± 995,8 pg/mL x 8126,0 ± 788,6, p=0,0342, Mann-Whitney’s test) increased after BCG on contacts of paucibacillary patients. The production of IFN-gamma and IL-10 against two synthetic peptides (p38 and p67), before and after BCG vaccine, were similar as seen with MLT antigen, but the absence of cytokine production against p69 help to identify this peptide as a effective diagnostic marker.Estudos em diferentes populações têm mostrado que a vacinação com a BCG concede proteção parcial contra a hanseníase. Entretanto, a necessidade da revacinação e seu impacto na resposta imune contra antígenos do Mycobacterium leprae é pouco compreendida, principalmente nas crianças que são contatos destes pacientes com hanseníase, e constituem uma população de risco para o adoecimento. Neste estudo, investigamos o papel da revacinação com a BCG na modulação da resposta imune em contatos menores de quinze anos de pacientes com hanseníase. Amostras de sangue periférico de vinte e cinco crianças, vacinadas com a BCG ao nascer, foram coletas antes e dois meses após a revacinação com a BCG. Suas células mononucleares (PBMC) foram estimuladas com proteínas e peptídeos do M. leprae, dosando-se as quantidades de IFN-γ e IL-10 por ELISA. Anteriormente a revacinação, foram coletas amostras para dosagem de IgM e IgG anti-PGL-1 e realizado o teste tuberculínico (PPD) para avaliação da eficácia vacinal após a revacinação. Identificamos o potencial papel da BCG em estimular a produção de IFN-γ e/ou IL-10 dependendo do antígeno e da idade do contato, sem relação com o PPD e sorologia anti-PGL-1. Estes resultados sugerem que a BCG modula a resposta imune dos contatos frente aos antígenos do M. leprae, com o frequente aumento nos níveis de IFN-γ, frente ao MLT, nas crianças com idade entre 1 a 4 anos (4.203 + 539,2 pg/mL pré-BCG x 9.141 + 860,9 pg/mL pós-BCG, p=0,001). Já os contatos entre 5 a 9 anos mostram aumento na produção de IL-10 (210 +39,4 pg/mL pré-BCG x 680 + 76,74 pg/mL pós-BCG, p=0,001) e IFN-γ (4.912 + 1.065 pg/mL x 9.249 + 1.171 pg/mL, p=0,024). Estes achados são acompanhados com o aumento dos casos de hanseníase em crianças entre 5 a 9 anos na comunidade. Entretanto, nos contatos entre 10 a 15 anos a revacinação falha em induzir o aumento de IFN-γ e IL-10. Os contatos de MB produziram, simultaneamente, altos níveis de IFN-γ e IL-10 em resposta ao MLT, enquanto que os contatos de PB somente produziram altos níveis de IFN-γ após a revacinação. A produção de IFN-γ e IL-10 frente aos peptídeos sintéticos p38 e p69 indica que estes antígenos podem ser possíveis marcadores de infecção. Assim, a BCG pode ser protetora, sendo eficiente em induzir o aumento da resposta por IFN-γ nestes contatos, frente aos antígenos do M. leprae.Câmara , Lilia Maria CarneiroFerreira, Emerson Ramalho2013-04-29T16:29:51Z2013-04-29T16:29:51Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfFERREIRA, E. R. A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase. 2010. 104 f. Dissertação (Mestrado em Microbiologia Médica) - Universidade Federal do Ceará. Faculdade de Medicina, Fortaleza, 2010.http://www.repositorio.ufc.br/handle/riufc/4787porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2021-02-09T14:58:59Zoai:repositorio.ufc.br:riufc/4787Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:17:27.764910Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase Revaccination with BCG modulates cytokine production against antigens of Mycobacterium leprae in contacts under 15 years of leprosy patients |
title |
A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase |
spellingShingle |
A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase Ferreira, Emerson Ramalho Hanseníase Mycobacterium bovis Vacina BCG Citocinas |
title_short |
A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase |
title_full |
A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase |
title_fullStr |
A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase |
title_full_unstemmed |
A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase |
title_sort |
A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase |
author |
Ferreira, Emerson Ramalho |
author_facet |
Ferreira, Emerson Ramalho |
author_role |
author |
dc.contributor.none.fl_str_mv |
Câmara , Lilia Maria Carneiro |
dc.contributor.author.fl_str_mv |
Ferreira, Emerson Ramalho |
dc.subject.por.fl_str_mv |
Hanseníase Mycobacterium bovis Vacina BCG Citocinas |
topic |
Hanseníase Mycobacterium bovis Vacina BCG Citocinas |
description |
Studies in different populations have shown that vaccination with BCG provides partial protection against leprosy. However, need of boost BCG vaccination and your impact in response immune against antigens of Mycobacterium leprae is poorly understood, mainly in children who are contacts of these patients with leprosy, and constitute population of risk for illness. This study, we investigated the paper a second dose of BCG given to contacts of leprosy patients under the age of fifteen years in modulate the response imune. Blood samples of twenty five children, who received a first dose of BCG at birth, were collected immediately before and two months after BCG revaccination. Your peripheral blood mononuclear cells (PBMC) stimulated by proteins and peptides of M. leprae, were measured IFN-γ and IL-10 by an ELISA assay. Before revaccination the serum anti-PGL1 IgG and IgM isotypes were measured by ELISA assays performed with native PGL1- coated microplates, and PPD-skin reaction was measured 2 to 3 months after revaccination. The study was approved by the local Ethic Committee, number 006-08, May 7th, 2008. BCG revaccination can induces IFN-gamma and/or IL-10 production related to antigen and age, but did not correlated with PPD-skin reaction or anti-PGL1 levels. The PBMC’s production of IFN-gamma against MLT (total M. leprae antigen) after BCG was higher on children under 4 years old (N= 8, 4203,0 ± 539,2 pg/mL before BCG x 9141,0 ± 860,9 pg/mL after BCG, p=0,015, Mann-Whitney’s test). Children between 5 and 9 years old showed an increase either of IFN-gama levels (N=11, 4912 ± 1065 pg/mL x 9249,0 ± 1171 pg/mL, p=0,024, Mann-Whitney’s test) or IL-10 levels (N=11, 210,6 ± 39,4 pg/mL x 680,3 ± 76,74 pg/mL, p=0, 0,001, Mann-Whitney’s test). However children between 10 and 15 years old failed to increase the cytokines levels after BCG booster. Contacts of multibacillary patients produced higher IFN-gamma levels (N= 13, 4536,0 ± 543,1 pg/mL x 9263,0 ± 989,0 pg/mL, p=0,0012, Mann-Whitney’s test) and IL-10 levels (N= 13, 235,9 ± 46,5 pg/mL x 743,5 ± 87,8, p=0,0012, Mann-Whitney’s test) against MLT after BCG, but only IFN-gamma levels (N=12, 4975,0 ± 995,8 pg/mL x 8126,0 ± 788,6, p=0,0342, Mann-Whitney’s test) increased after BCG on contacts of paucibacillary patients. The production of IFN-gamma and IL-10 against two synthetic peptides (p38 and p67), before and after BCG vaccine, were similar as seen with MLT antigen, but the absence of cytokine production against p69 help to identify this peptide as a effective diagnostic marker. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2013-04-29T16:29:51Z 2013-04-29T16:29:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FERREIRA, E. R. A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase. 2010. 104 f. Dissertação (Mestrado em Microbiologia Médica) - Universidade Federal do Ceará. Faculdade de Medicina, Fortaleza, 2010. http://www.repositorio.ufc.br/handle/riufc/4787 |
identifier_str_mv |
FERREIRA, E. R. A revacinação com a BCG modula a produção de citocinas frente a antígenos do Mycobacterium leprae, em contatos menores de 15 anos de pacientes com hanseníase. 2010. 104 f. Dissertação (Mestrado em Microbiologia Médica) - Universidade Federal do Ceará. Faculdade de Medicina, Fortaleza, 2010. |
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http://www.repositorio.ufc.br/handle/riufc/4787 |
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Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
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