Antigens of Coccidioides posadasii as an Important Tool for the Immunodiagnosis of Coccidioidomycosis

• Autor(a) principal: Medrano, Delia Jessica Astete
• Data de Publicação: 2013
• Outros Autores: Cordeiro, Rossana de Aguiar, Patoilo, Kharla Rabelo Nobre, Praciano, Silviane Bandeira, Marques, Francisca Jakelyne de Farias, Martins, Liline Maria Soares, Eulalio, Kelsen Dantas, Deus Filho, Antônio de, Brilhante, Raimunda Samia Nogueira, Camargo, Zoilo Pires de, Rocha, Marcos Fábio Gadelha, Sidrim, José Júlio Costa, Fechine, Maria Auxiliadora Bezerra, Cavalvanti, Maria do Amparo Salmito
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/4937
• topic: Coccidioidomicose; Técnicas Imunoenzimáticas; Imunodifusão

Serologic diagnosis has been presented as a safe alternative for coccidioidomycosis. However, commercial kits based on coccidioidal antibodies available in the USA are considered too expensive for laboratories outside that country. In this study, we describe the preparation of antigens for detection of human coccidioidal antibodies by the immunodiffusion test (ID) and enzyme immunoassay (EIA). Antigens were tested against serum samples from patients with coccidioidomycosis, histoplasmosis and paracoccidioidomycosis, as well as healthy individuals. The highest reactivity in the ID tests was seen in the F0-90 antigen. In the EIAs, the best results were obtained with the F60-90 antigen. None of the serum samples from healthy individuals were recognized by any of the antigen extracts tested by ID or EIA. In conclusion, the F0-90 and F60-90 antigens have the potential to be commercially employed in presumptive diagnosis of coccidioidomycosis by ID or EIA, respectively. The tests could improve serological diagnosis of coccidioidomycosis in South America.