Farnesol against Coccidioides posadasii : its effect on growth, ergosterol
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/8551 |
Resumo: | Coccidioidomycosis is a systemic mycosis caused by the dimorphic fungi Coccidioides spp. The treatment for chronic and/or disseminated coccidioidomycosis can be prolonged and complicated. Therefore, the search for new drugs is necessary. Farnesol is a precursor in the sterol biosynthesis pathway that has been shown to present antifungal activity. Thus, the objective of this study was to evaluate the in vitro antifungal activity of farnesol alone and in combination with antifungal agents against clinical and environmental strains of Coccidioides posadasii, as well as to determine their effect on the synthesis of ergosterol and on cell permeability. This study employed the broth macrodilution method to determine the minimum inhibitory concentration (MIC) of farnesol against strains of C. posadasii. Quantification of ergosterol was performed with ten strains of C. posadasii, after the exposure to sub-inhibitory concentrations of farnesol. Finally, the activity of farnesol was evaluated at the presence of osmotic stress, induced by the addition of NaCl to the culture medium, during the susceptibility tests. The results showed that farnesol exhibited low MICs (ranging from 0,00171- 0,01369 mg/L) against all tested strains. The combination of farnesol with the antifungals showed synergistic effects (FICI≤0.5). As for ergosterol quantification, it was observed that exposure to sub-inhibitory concentrations of farnesol decreased the amount of ergosterol extracted from the fungal cells. Furthermore, farnesol also showed lower MIC values when the strains were subjected to osmotic stress, indicating the action of this compound on the fungal membrane. Thus, due to the high in vitro antifungal activity, this work brings perspectives for the performance of in vivo studies to further elucidate the effects of farnesol on the host cells. |
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Farnesol against Coccidioides posadasii : its effect on growth, ergosterolCoccidioidomicoseErgosterolCoccidioidomycosis is a systemic mycosis caused by the dimorphic fungi Coccidioides spp. The treatment for chronic and/or disseminated coccidioidomycosis can be prolonged and complicated. Therefore, the search for new drugs is necessary. Farnesol is a precursor in the sterol biosynthesis pathway that has been shown to present antifungal activity. Thus, the objective of this study was to evaluate the in vitro antifungal activity of farnesol alone and in combination with antifungal agents against clinical and environmental strains of Coccidioides posadasii, as well as to determine their effect on the synthesis of ergosterol and on cell permeability. This study employed the broth macrodilution method to determine the minimum inhibitory concentration (MIC) of farnesol against strains of C. posadasii. Quantification of ergosterol was performed with ten strains of C. posadasii, after the exposure to sub-inhibitory concentrations of farnesol. Finally, the activity of farnesol was evaluated at the presence of osmotic stress, induced by the addition of NaCl to the culture medium, during the susceptibility tests. The results showed that farnesol exhibited low MICs (ranging from 0,00171- 0,01369 mg/L) against all tested strains. The combination of farnesol with the antifungals showed synergistic effects (FICI≤0.5). As for ergosterol quantification, it was observed that exposure to sub-inhibitory concentrations of farnesol decreased the amount of ergosterol extracted from the fungal cells. Furthermore, farnesol also showed lower MIC values when the strains were subjected to osmotic stress, indicating the action of this compound on the fungal membrane. Thus, due to the high in vitro antifungal activity, this work brings perspectives for the performance of in vivo studies to further elucidate the effects of farnesol on the host cells.Antimicrobial Agents and Chemotherapy2014-07-29T13:46:37Z2014-07-29T13:46:37Z2013-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBRILHANTE, R. S. N. et al. Farnesol against Coccidioides posadasii : its effect on growth, ergosterol. Antimicrobial Agents and Chemotherapy, v. 57, n. 5, p. 2167-2170, mar. 2013.1098-6596 On linehttp://www.repositorio.ufc.br/handle/riufc/8551Brilhante, Raimunda Sâmia NogueiraLima, Rita Amanda Chaves deCaetano, Erica PachecoLeite, João Jaime GiffoniCastelo-Branco, Débora de Souza Collares MaiaRibeiro, Joyce FontelesBandeira, Tereza de Jesus Pinheiro GomesCordeiro, Rossana de AguiarMonteiro, André JallesSidrim, José Júlio CostaRocha, Marcos Fábio Gadelhaengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-01-18T18:40:15Zoai:repositorio.ufc.br:riufc/8551Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:25:51.591933Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Farnesol against Coccidioides posadasii : its effect on growth, ergosterol |
title |
Farnesol against Coccidioides posadasii : its effect on growth, ergosterol |
spellingShingle |
Farnesol against Coccidioides posadasii : its effect on growth, ergosterol Brilhante, Raimunda Sâmia Nogueira Coccidioidomicose Ergosterol |
title_short |
Farnesol against Coccidioides posadasii : its effect on growth, ergosterol |
title_full |
Farnesol against Coccidioides posadasii : its effect on growth, ergosterol |
title_fullStr |
Farnesol against Coccidioides posadasii : its effect on growth, ergosterol |
title_full_unstemmed |
Farnesol against Coccidioides posadasii : its effect on growth, ergosterol |
title_sort |
Farnesol against Coccidioides posadasii : its effect on growth, ergosterol |
author |
Brilhante, Raimunda Sâmia Nogueira |
author_facet |
Brilhante, Raimunda Sâmia Nogueira Lima, Rita Amanda Chaves de Caetano, Erica Pacheco Leite, João Jaime Giffoni Castelo-Branco, Débora de Souza Collares Maia Ribeiro, Joyce Fonteles Bandeira, Tereza de Jesus Pinheiro Gomes Cordeiro, Rossana de Aguiar Monteiro, André Jalles Sidrim, José Júlio Costa Rocha, Marcos Fábio Gadelha |
author_role |
author |
author2 |
Lima, Rita Amanda Chaves de Caetano, Erica Pacheco Leite, João Jaime Giffoni Castelo-Branco, Débora de Souza Collares Maia Ribeiro, Joyce Fonteles Bandeira, Tereza de Jesus Pinheiro Gomes Cordeiro, Rossana de Aguiar Monteiro, André Jalles Sidrim, José Júlio Costa Rocha, Marcos Fábio Gadelha |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Brilhante, Raimunda Sâmia Nogueira Lima, Rita Amanda Chaves de Caetano, Erica Pacheco Leite, João Jaime Giffoni Castelo-Branco, Débora de Souza Collares Maia Ribeiro, Joyce Fonteles Bandeira, Tereza de Jesus Pinheiro Gomes Cordeiro, Rossana de Aguiar Monteiro, André Jalles Sidrim, José Júlio Costa Rocha, Marcos Fábio Gadelha |
dc.subject.por.fl_str_mv |
Coccidioidomicose Ergosterol |
topic |
Coccidioidomicose Ergosterol |
description |
Coccidioidomycosis is a systemic mycosis caused by the dimorphic fungi Coccidioides spp. The treatment for chronic and/or disseminated coccidioidomycosis can be prolonged and complicated. Therefore, the search for new drugs is necessary. Farnesol is a precursor in the sterol biosynthesis pathway that has been shown to present antifungal activity. Thus, the objective of this study was to evaluate the in vitro antifungal activity of farnesol alone and in combination with antifungal agents against clinical and environmental strains of Coccidioides posadasii, as well as to determine their effect on the synthesis of ergosterol and on cell permeability. This study employed the broth macrodilution method to determine the minimum inhibitory concentration (MIC) of farnesol against strains of C. posadasii. Quantification of ergosterol was performed with ten strains of C. posadasii, after the exposure to sub-inhibitory concentrations of farnesol. Finally, the activity of farnesol was evaluated at the presence of osmotic stress, induced by the addition of NaCl to the culture medium, during the susceptibility tests. The results showed that farnesol exhibited low MICs (ranging from 0,00171- 0,01369 mg/L) against all tested strains. The combination of farnesol with the antifungals showed synergistic effects (FICI≤0.5). As for ergosterol quantification, it was observed that exposure to sub-inhibitory concentrations of farnesol decreased the amount of ergosterol extracted from the fungal cells. Furthermore, farnesol also showed lower MIC values when the strains were subjected to osmotic stress, indicating the action of this compound on the fungal membrane. Thus, due to the high in vitro antifungal activity, this work brings perspectives for the performance of in vivo studies to further elucidate the effects of farnesol on the host cells. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03 2014-07-29T13:46:37Z 2014-07-29T13:46:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BRILHANTE, R. S. N. et al. Farnesol against Coccidioides posadasii : its effect on growth, ergosterol. Antimicrobial Agents and Chemotherapy, v. 57, n. 5, p. 2167-2170, mar. 2013. 1098-6596 On line http://www.repositorio.ufc.br/handle/riufc/8551 |
identifier_str_mv |
BRILHANTE, R. S. N. et al. Farnesol against Coccidioides posadasii : its effect on growth, ergosterol. Antimicrobial Agents and Chemotherapy, v. 57, n. 5, p. 2167-2170, mar. 2013. 1098-6596 On line |
url |
http://www.repositorio.ufc.br/handle/riufc/8551 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Antimicrobial Agents and Chemotherapy |
publisher.none.fl_str_mv |
Antimicrobial Agents and Chemotherapy |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
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1813028800168984576 |