Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos

Detalhes bibliográficos
Autor(a) principal: Pontes, Ana Georgina Oliveira
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/16370
Resumo: Pombalia calceolaria L., Violaceae, popularly known as ipecac-the-beach, ipecabranca, ipecacunha of healers is a perennial, herbaceous predominant in the northeastern backlands. Although there are no data in the literature proving their pharmacological activity, for decades, its roots are prepared in the form of decoction, licker and maceration, the main indications are popular cough, expectoration, as anthelmintic, antidiarrheal and dentition. This study aimed to carry out the Pharmacognostic characterization of P. roots calceolaria the botanical, chemical and pharmacological aspects. The importance of morphoanatomical is the fact that there is no record in the literature describing the botanical profile of this plant species. Thus, the roots of P. recémcoletadas calceolaria were characterized morphologically with the naked eye and then held the anatomical characterization by histological section, immunohistochemical reaction photomicrographic record in clear light field, where it was shown that the root study presents the secondary xylem originating exchange with dimorphism in the size of vessels and phloem consists of few layers surrounding the xylem. In cortical parenchyma was visualized and fotomicrografado under polarized light, RESENCE inulin crystals. The phytochemical study was preceded by preliminary tests to establish the plant drug, where the quality specifications were obtained, as the determination of yield from plant drugs, residual moisture, the content of total ash, followed by phytochemical screening has resulted mainly in presence of saponins, reducing sugars and steroids. Then, the aqueous extract obtained from the decoction was divided into two samples where one was lyophilized and through the other, was obtained by precipitation in the presence of methanol, a polysaccharide with a content of 13.0% w / w, and this subjected to analysis by chromatographic techniques such as HPLC and GPC, which indicated the presence of fructose and glucose, and the molecular weight of 4.0 x 10³ Da, respectively. Subsequently, after obtaining uni- and two-dimensional NMR spectra of ¹H, ¹³C, DEPT, HSQC and 135, the chemical structure of the polysaccharide was identified as inulin. The pharmacological tests were performed on isolated rat trachea with both the polysaccharide and with the lyophilized aqueous extract of the roots of P. calceolaria (EALPC) to measure the relaxant activity on the contractility of the body. After addition of CCh 1μM to induce sustained contraction in isolated organ in vats system in test with polysaccharide there was no significant relaxation in the sustained contraction. Testing conducted EALPC was observed relaxation of contraction, which showed an effective reduction of the contractile response of EALPC of approximately 20% compared to control, featuring a mild bronchodilator activity.
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spelling Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicosPharmacognosy study of the roots of ipecac-the-beach (Pombalia calceolaria (L.) Paula-Souza): botanical aspects, chemical and pharmacologicalRaízes de PlantasInsulinaFarmacognosiaPombalia calceolaria L., Violaceae, popularly known as ipecac-the-beach, ipecabranca, ipecacunha of healers is a perennial, herbaceous predominant in the northeastern backlands. Although there are no data in the literature proving their pharmacological activity, for decades, its roots are prepared in the form of decoction, licker and maceration, the main indications are popular cough, expectoration, as anthelmintic, antidiarrheal and dentition. This study aimed to carry out the Pharmacognostic characterization of P. roots calceolaria the botanical, chemical and pharmacological aspects. The importance of morphoanatomical is the fact that there is no record in the literature describing the botanical profile of this plant species. Thus, the roots of P. recémcoletadas calceolaria were characterized morphologically with the naked eye and then held the anatomical characterization by histological section, immunohistochemical reaction photomicrographic record in clear light field, where it was shown that the root study presents the secondary xylem originating exchange with dimorphism in the size of vessels and phloem consists of few layers surrounding the xylem. In cortical parenchyma was visualized and fotomicrografado under polarized light, RESENCE inulin crystals. The phytochemical study was preceded by preliminary tests to establish the plant drug, where the quality specifications were obtained, as the determination of yield from plant drugs, residual moisture, the content of total ash, followed by phytochemical screening has resulted mainly in presence of saponins, reducing sugars and steroids. Then, the aqueous extract obtained from the decoction was divided into two samples where one was lyophilized and through the other, was obtained by precipitation in the presence of methanol, a polysaccharide with a content of 13.0% w / w, and this subjected to analysis by chromatographic techniques such as HPLC and GPC, which indicated the presence of fructose and glucose, and the molecular weight of 4.0 x 10³ Da, respectively. Subsequently, after obtaining uni- and two-dimensional NMR spectra of ¹H, ¹³C, DEPT, HSQC and 135, the chemical structure of the polysaccharide was identified as inulin. The pharmacological tests were performed on isolated rat trachea with both the polysaccharide and with the lyophilized aqueous extract of the roots of P. calceolaria (EALPC) to measure the relaxant activity on the contractility of the body. After addition of CCh 1μM to induce sustained contraction in isolated organ in vats system in test with polysaccharide there was no significant relaxation in the sustained contraction. Testing conducted EALPC was observed relaxation of contraction, which showed an effective reduction of the contractile response of EALPC of approximately 20% compared to control, featuring a mild bronchodilator activity.Pombalia calceolaria L., Violaceae, popularmente conhecida por ipeca-da-praia, ipecabranca, ipecacunha dos raizeiros é uma herbácea perene, predominante no sertão nordestino. Embora não existam dados na literatura comprovando sua atividade farmacológica, por décadas, suas raízes são preparadas na forma de decocto, lambedor e maceração, cujas principais indicações populares são para tosse, expectoração, como vermífugo, antidiarréico e para dentição. O presente estudo teve por objetivo realizar a caracterização farmacognóstica das raízes de P. calceolaria nos aspectos botânicos, químicos e farmacológicos. A importância da caracterização morfoanatômica está no fato de que não há registro em literatura descrevendo o perfil botânico desta espécie vegetal. Assim, as raízes recémcoletadas de P. calceolaria foram caracterizadas morfologicamente com vista desarmada e em seguida realizada a caracterização anatômica através de secção histológica, reação histoquímica, registro fotomicrográfico em campo de luz claro, onde foi evidenciado que a raiz em estudo apresenta o xilema secundário oriundo do câmbio com dimorfismo no tamanho dos vasos e floema secundário constituído de poucas camadas circundando o xilema. No parênquima cortical foi visualizado e fotomicrografado, sob luz polarizada, a resença de cristais de inulina. O estudo fitoquímico foi precedido por ensaios preliminares para a obtenção da droga vegetal, onde foram obtidos as especificações de qualidade, como a determinação do rendimento da droga vegetal, da umidade residual, do teor de cinzas totais, seguida da prospecção fitoquímica que resultou majoritariamente em presença de saponinas, açúcares redutores e esteróides. Em seguida, o extrato aquoso obtido por decocção foi dividido em duas amostras, onde uma foi liofilizada e por meio da outra, foi obtido por precipitação na presença de metanol, um polissacarídeo, com teor de 13,0% p/p, sendo este submetido a análises por técnicas cromatográficas como CLAE e GPC, as quais indicaram a presença de frutose e glicose, e o peso molecular de 4,0 x 10³ Da, respectivamente. Posteriormente, após a obtenção de espectros uni- e bidimensionais de RMN de ¹H, ¹³C, DEPT-135 e HSQC, a estrutura química do polissacarídeo foi identificada como sendo inulina. Os testes farmacológicos foram realizados em traqueia isolada de rato tanto com o polissacarídeo quanto com o extrato aquoso liofilizado das raízes de P. calceolaria (EALPC) para avaliar a atividade miorrelaxante sobre a contratilidade do órgão. Após adição de CCh 1µM, para induzir contração sustentada no órgão isolado no sistema de cubas, em ensaio com o polissacarídeo houve ausência de relaxamento significativo na contração sustentada. No teste realizado com EALPC foi observado relaxamento da contração, o qual apresentou uma redução efetiva da resposta contrátil do EALPC de aproximadamente 20% em relação ao controle, caracterizando uma leve atividade broncodilatadora.Bandeira, Mary Anne MedeirosPontes, Ana Georgina Oliveira2016-04-25T12:26:58Z2016-04-25T12:26:58Z2015-12-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfPONTES, A. G. O. Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos. 2015. 94 f. Dissertação (Mestrado em Ciências Farmacêuticas) - faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal do Ceará, Fortaleza, 2015.http://www.repositorio.ufc.br/handle/riufc/16370porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2018-12-27T13:48:09Zoai:repositorio.ufc.br:riufc/16370Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:54:44.241366Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos
Pharmacognosy study of the roots of ipecac-the-beach (Pombalia calceolaria (L.) Paula-Souza): botanical aspects, chemical and pharmacological
title Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos
spellingShingle Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos
Pontes, Ana Georgina Oliveira
Raízes de Plantas
Insulina
Farmacognosia
title_short Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos
title_full Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos
title_fullStr Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos
title_full_unstemmed Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos
title_sort Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos
author Pontes, Ana Georgina Oliveira
author_facet Pontes, Ana Georgina Oliveira
author_role author
dc.contributor.none.fl_str_mv Bandeira, Mary Anne Medeiros
dc.contributor.author.fl_str_mv Pontes, Ana Georgina Oliveira
dc.subject.por.fl_str_mv Raízes de Plantas
Insulina
Farmacognosia
topic Raízes de Plantas
Insulina
Farmacognosia
description Pombalia calceolaria L., Violaceae, popularly known as ipecac-the-beach, ipecabranca, ipecacunha of healers is a perennial, herbaceous predominant in the northeastern backlands. Although there are no data in the literature proving their pharmacological activity, for decades, its roots are prepared in the form of decoction, licker and maceration, the main indications are popular cough, expectoration, as anthelmintic, antidiarrheal and dentition. This study aimed to carry out the Pharmacognostic characterization of P. roots calceolaria the botanical, chemical and pharmacological aspects. The importance of morphoanatomical is the fact that there is no record in the literature describing the botanical profile of this plant species. Thus, the roots of P. recémcoletadas calceolaria were characterized morphologically with the naked eye and then held the anatomical characterization by histological section, immunohistochemical reaction photomicrographic record in clear light field, where it was shown that the root study presents the secondary xylem originating exchange with dimorphism in the size of vessels and phloem consists of few layers surrounding the xylem. In cortical parenchyma was visualized and fotomicrografado under polarized light, RESENCE inulin crystals. The phytochemical study was preceded by preliminary tests to establish the plant drug, where the quality specifications were obtained, as the determination of yield from plant drugs, residual moisture, the content of total ash, followed by phytochemical screening has resulted mainly in presence of saponins, reducing sugars and steroids. Then, the aqueous extract obtained from the decoction was divided into two samples where one was lyophilized and through the other, was obtained by precipitation in the presence of methanol, a polysaccharide with a content of 13.0% w / w, and this subjected to analysis by chromatographic techniques such as HPLC and GPC, which indicated the presence of fructose and glucose, and the molecular weight of 4.0 x 10³ Da, respectively. Subsequently, after obtaining uni- and two-dimensional NMR spectra of ¹H, ¹³C, DEPT, HSQC and 135, the chemical structure of the polysaccharide was identified as inulin. The pharmacological tests were performed on isolated rat trachea with both the polysaccharide and with the lyophilized aqueous extract of the roots of P. calceolaria (EALPC) to measure the relaxant activity on the contractility of the body. After addition of CCh 1μM to induce sustained contraction in isolated organ in vats system in test with polysaccharide there was no significant relaxation in the sustained contraction. Testing conducted EALPC was observed relaxation of contraction, which showed an effective reduction of the contractile response of EALPC of approximately 20% compared to control, featuring a mild bronchodilator activity.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-14
2016-04-25T12:26:58Z
2016-04-25T12:26:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv PONTES, A. G. O. Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos. 2015. 94 f. Dissertação (Mestrado em Ciências Farmacêuticas) - faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal do Ceará, Fortaleza, 2015.
http://www.repositorio.ufc.br/handle/riufc/16370
identifier_str_mv PONTES, A. G. O. Estudo farmacognóstico das raízes de ipeca-da-praia (Pombalia calceolaria (L.) Paula-Souza): aspectos botânicos, químicos e farmacológicos. 2015. 94 f. Dissertação (Mestrado em Ciências Farmacêuticas) - faculdade de Farmácia, Odontologia e Enfermagem, Universidade Federal do Ceará, Fortaleza, 2015.
url http://www.repositorio.ufc.br/handle/riufc/16370
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instname:Universidade Federal do Ceará (UFC)
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instname_str Universidade Federal do Ceará (UFC)
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reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
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repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
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