Easy storage strategies for sporothrix spp. strains

• Autor(a) principal: Brilhante, Raimunda Sâmia Nogueira
• Data de Publicação: 2015
• Outros Autores: Silva, Natalya Fechine, Lima, Rita Amanda Chaves de, Caetano, Érica Pacheco, Alencar, Lucas Pereira de, Castelo-Branco, Débora de Souza Collares Maia, Moreira, José Luciano Bezerra, Bandeira, Silviane Praciano, Camargo, Zoilo Pires de, Rodrigues, Anderson Messias, Bandeira, Tereza de Jesus Pinheiro Gomes, Monteiro, André Jalles, Cordeiro, Rossana de Aguiar, Sidrim, José Júlio Costa, Rocha, Marcos Fábio Gadelha
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/17895
• topic: Sporothrix; Glicerol; Lactose

The present study evaluated the maintenance of Sporothrix spp. (6 Sporothrix brasiliensis;6S. schenckii;5 S. mexicana, and 3 S. globosa) in saline at 4 C, and in 10% glycerol plus either 10% lactose or 10% sucrose, at -20 C and -80 C. Viability was assessed after 3, 6, and 9 months of storage, through the recovery of strains on potato dextrose agar and analysis of macro- and micromorphological features. Conidium quantification was performed before and after storage, at 3, 6 and 9 months. 100% viability was observed, regardless of storage conditions or time period. Storage at 4 C and at -20 C did not alter the number of conidia, but lower conidium counts were observed at -80 C. This study shows that the combination of glycerol with lactose or sucrose is effective to maintain Sporothrix spp. at freezing temperatures.