Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta)

Detalhes bibliográficos
Autor(a) principal: Ibrahim, Abdulrazak Baba
Data de Publicação: 2008
Outros Autores: Nogueira, Fabio C. S., Pinheiro, Camila B., Brasil, Juliana N., Jereissati, Emmanuel S., Jucá, Thiago Lustosa, Soares, Arlete A., Santos, Marise F., Domont, Gilberto B., Campos, Francisco A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/59063
Resumo: Using histological analysis of the induction of secondary somatic embryogenesis (SSE) in cassava (Manihot esculenta Crantz) as a guide, we performed 2-DE for protein separation and matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry (MALDI-TOF-TOF-MS) for protein identification in cotyledons of cassava somatic embryos undergoing SSE. Reference map obtained by 2- DE within a pH range of 3–10 and a size range of 6–97 kDa revealed approximately 410 eletrophoretically resolved spots populated primarily by acidic (pI < 7) proteins with molecular masses between 30 and 75 kDa. Tryptic digestion of 163 of the most abundant spots led to the identification of 86 proteins with a protein identification success rate of 53%. In total, 43% of the identified proteins were involved in metabolism and energy and 11.6% in protein destination and storage. Others are, disease/defense (11.6%), transcription and protein synthesis (7%), signal transduction (5.8%), cell growth/division (3.5%), transporters (3.5%), cell structure (2.3%), secondary metabolism (1.2%) and other functional classes (10.5%). Our studies demonstrate that 2-DE-based proteomic approaches combined with histological studies can serve as tools for identifying protein markers for the developmental stages of cassava SE while providing clues on the underlying causes of the low rate of conversion of cassava somatic embryos into mature plants.
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spelling Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta)Manihot esculentaProteome analysisMass spectrometrySomatic embryogenesisSecondary somatic embryogenesisUsing histological analysis of the induction of secondary somatic embryogenesis (SSE) in cassava (Manihot esculenta Crantz) as a guide, we performed 2-DE for protein separation and matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry (MALDI-TOF-TOF-MS) for protein identification in cotyledons of cassava somatic embryos undergoing SSE. Reference map obtained by 2- DE within a pH range of 3–10 and a size range of 6–97 kDa revealed approximately 410 eletrophoretically resolved spots populated primarily by acidic (pI < 7) proteins with molecular masses between 30 and 75 kDa. Tryptic digestion of 163 of the most abundant spots led to the identification of 86 proteins with a protein identification success rate of 53%. In total, 43% of the identified proteins were involved in metabolism and energy and 11.6% in protein destination and storage. Others are, disease/defense (11.6%), transcription and protein synthesis (7%), signal transduction (5.8%), cell growth/division (3.5%), transporters (3.5%), cell structure (2.3%), secondary metabolism (1.2%) and other functional classes (10.5%). Our studies demonstrate that 2-DE-based proteomic approaches combined with histological studies can serve as tools for identifying protein markers for the developmental stages of cassava SE while providing clues on the underlying causes of the low rate of conversion of cassava somatic embryos into mature plants.Plant Science2021-06-17T16:33:16Z2021-06-17T16:33:16Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBABA, Abdulrazak I. et al. Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta). Plant Science, [s.l.], v. 175, p. 717-723, 2008.0168-9452http://www.repositorio.ufc.br/handle/riufc/59063Ibrahim, Abdulrazak BabaNogueira, Fabio C. S.Pinheiro, Camila B.Brasil, Juliana N.Jereissati, Emmanuel S.Jucá, Thiago LustosaSoares, Arlete A.Santos, Marise F.Domont, Gilberto B.Campos, Francisco A. P.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFC2023-10-10T19:40:35Zoai:repositorio.ufc.br:riufc/59063Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:53:33.852769Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta)
title Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta)
spellingShingle Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta)
Ibrahim, Abdulrazak Baba
Manihot esculenta
Proteome analysis
Mass spectrometry
Somatic embryogenesis
Secondary somatic embryogenesis
title_short Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta)
title_full Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta)
title_fullStr Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta)
title_full_unstemmed Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta)
title_sort Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta)
author Ibrahim, Abdulrazak Baba
author_facet Ibrahim, Abdulrazak Baba
Nogueira, Fabio C. S.
Pinheiro, Camila B.
Brasil, Juliana N.
Jereissati, Emmanuel S.
Jucá, Thiago Lustosa
Soares, Arlete A.
Santos, Marise F.
Domont, Gilberto B.
Campos, Francisco A. P.
author_role author
author2 Nogueira, Fabio C. S.
Pinheiro, Camila B.
Brasil, Juliana N.
Jereissati, Emmanuel S.
Jucá, Thiago Lustosa
Soares, Arlete A.
Santos, Marise F.
Domont, Gilberto B.
Campos, Francisco A. P.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ibrahim, Abdulrazak Baba
Nogueira, Fabio C. S.
Pinheiro, Camila B.
Brasil, Juliana N.
Jereissati, Emmanuel S.
Jucá, Thiago Lustosa
Soares, Arlete A.
Santos, Marise F.
Domont, Gilberto B.
Campos, Francisco A. P.
dc.subject.por.fl_str_mv Manihot esculenta
Proteome analysis
Mass spectrometry
Somatic embryogenesis
Secondary somatic embryogenesis
topic Manihot esculenta
Proteome analysis
Mass spectrometry
Somatic embryogenesis
Secondary somatic embryogenesis
description Using histological analysis of the induction of secondary somatic embryogenesis (SSE) in cassava (Manihot esculenta Crantz) as a guide, we performed 2-DE for protein separation and matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry (MALDI-TOF-TOF-MS) for protein identification in cotyledons of cassava somatic embryos undergoing SSE. Reference map obtained by 2- DE within a pH range of 3–10 and a size range of 6–97 kDa revealed approximately 410 eletrophoretically resolved spots populated primarily by acidic (pI < 7) proteins with molecular masses between 30 and 75 kDa. Tryptic digestion of 163 of the most abundant spots led to the identification of 86 proteins with a protein identification success rate of 53%. In total, 43% of the identified proteins were involved in metabolism and energy and 11.6% in protein destination and storage. Others are, disease/defense (11.6%), transcription and protein synthesis (7%), signal transduction (5.8%), cell growth/division (3.5%), transporters (3.5%), cell structure (2.3%), secondary metabolism (1.2%) and other functional classes (10.5%). Our studies demonstrate that 2-DE-based proteomic approaches combined with histological studies can serve as tools for identifying protein markers for the developmental stages of cassava SE while providing clues on the underlying causes of the low rate of conversion of cassava somatic embryos into mature plants.
publishDate 2008
dc.date.none.fl_str_mv 2008
2021-06-17T16:33:16Z
2021-06-17T16:33:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BABA, Abdulrazak I. et al. Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta). Plant Science, [s.l.], v. 175, p. 717-723, 2008.
0168-9452
http://www.repositorio.ufc.br/handle/riufc/59063
identifier_str_mv BABA, Abdulrazak I. et al. Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta). Plant Science, [s.l.], v. 175, p. 717-723, 2008.
0168-9452
url http://www.repositorio.ufc.br/handle/riufc/59063
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Plant Science
publisher.none.fl_str_mv Plant Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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