Perfil de susceptibilidade antimicrobiana e preliminar de virulência entre cepas de Vibrio spp. isoladas da água e sedimento do estuário do Rio Acaraú, Ceará, Brasil

• Autor(a) principal: Rocha, Rafael dos Santos
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/1130
• topic: Vibrionaceae; Ambiente; Engenharia de pesca; Antimicrobianos; Patogenicidade

The estuarine environment is recognized as an intake of contaminated water from different sources. The genus Vibrio is found in those places, being reported in many papers resistant strains to antibiotics used routinely in medical treatment. Seventy strains of Vibrio were selected originated from samples of water and sediment from the Acaraú estuary, West coast of Ceará, Brazil to experiment. The antibiogram was proposed in two batteries, one with Mueller Hinton agar dissolved in distilled water (MH+DW) and the other in seawater (MH+SW), and is tested the presence of virulence factors protease (caseinase, elastase and gelatinase), lipases, phospholipases, DNases, amylase, urease, and hemolysin (β-hemolysis test by Kanagawa Wagatsuma agar). The antimicrobials tested were NAL, AMP, ATM, CFL, CIP, CHL, CTX, STP, GEN, OTC, PEN, TCY and STX. In MH+DW, the resistance profile was observed for 47 (67.1%) strains to PEN, 26 (37.1%) to AMP and CFL, 8 (11.4%) to OTC, 6 (8.6%) to TCY, 3 (4.3%) to ATM and 1 (1.4%) to CHL, while for MH+SW, 68 (97.1%) strains were resistant to OTC, 67 (95.7%) to TCY, 57 (81.4%) to PEN, 34 (48.6%) to CFL, 29 (41.4%) to AMP , 21 (30.0%) to STP, 20 (28.5%) to GEN, 5 (7.2%) to ATM and NAL, 3 (4.3%) to STX and 1 (1.4%) to CIP. The resistant strains in MH+DW were subjected to plasmid curing by acridine orange agent. Resistance plasmid was observed in 36.21% of resistant strains to AMP, 5.17% to ATM, 37.93% to CFL, 13.79% to OTC, 53.45% to PEN and 6.90% to TCY. By order of recurrence, 67 (91.42%) strains of Vibrio spp. have analyzed the enzyme urease, followed by 57 (81.42%) to lipase, 54 (77.14%) to amylase, 53 (75.72%) to gelatinase, 43 (61.42%) to caseinase, 26 (37.14%) to phospholipase, 17 (24.28%) to DNase, 16 (22.86%) to elastase and 13 (18.57%) to β-hemolysis (Kanagawa). Seawater influence significantly the characterization of the resistance of strains of Vibrio spp. analyzed mainly on the drugs of the tetracycline class, and has been detected multidrug resistant strains associated with preliminary virulence factors.