InvestigaÃÃo da aÃÃo central do timol em modelos comportamentais de ansiedade, depressÃo e convulsÃo em camundongos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=10762 |
Resumo: | Thymol (2-isopropyl-5-methyl-phenol) an isomer of Carvacrol. Present as large colorless crystals or white crystalline powder. It is a monoterpene essential oil extracted from various herbs, and the main component of the essential oil of rosemary-pepper (Lippia sidoides), constituting approximately 48% of its composition. This paper presents the behavioral actions of thymol in animal models of anxiety, depression, seizures and sedation, such as elevated plus maze (EPM), open field, rota rod, forced swim, tail suspension, seizure induced by pentylenetetrazol and pentobarbital-induced sleep time. Thymol was administered orally in male mice at doses of 25mg/kg and 50mg / kg. The results showed that thymol at both doses studied did cause muscula relaxament activits change locomotor activity on the test rota rod nor the number of crossings in the open field test, but reducedThe grooming was reduced with the administration of thymol in the open field test. At both doses studied LCE thymol increased all parameters observed suggesting a possible anxiolytic effect. Flumazenil, an antagonist of GABA / benzodiazepine, reversed this effect. In the test of sleep time induced by pentobarbital thymol not alter the latency but increased sleep duration. Although this test is sensitive to CNS depressant agents but is not specific for compounds which would interfere with the biotransformation of pentobarbital. In the test of pentylenetetrazol-induced seizures, thymol increased latency of seizure at the dose of 50mg/kg, however was not able to change the mortality of animals. Thymol showed antidepressant effect in the forced swim test and the tail suspension. This effect was reversed by pretreatment of animals with PCPA (a serotonin synthesis inhibitor), Prazosin and Yohimbine (adrenoreceptor antagonists), SCH23390 and Sulpiride (dopamine receptor antagonists). These results suggest that thymol shows anxiolytic effects probably associated with the GABAergic system and antidepressant effects associated with the serotonergic, noradrenergic and dopaminergic without causing sedation. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisInvestigaÃÃo da aÃÃo central do timol em modelos comportamentais de ansiedade, depressÃo e convulsÃo em camundongos2012-11-29Francisca ClÃa FlorenÃo de Sousa31636020372http://lattes.cnpq.br/1180465052181572Danielle MacÃdo Gaspar50160176387http://lattes.cnpq.br/1566937332957369Gisela Costa CamarÃo06001734372http://lattes.cnpq.br/881251533662289380764070363http://lattes.cnpq.br/6295544741163726Mariana Lima FernandesUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em FarmacologiaUFCBRAnxiety, Depression, ThymolFARMACOLOGIAThymol (2-isopropyl-5-methyl-phenol) an isomer of Carvacrol. Present as large colorless crystals or white crystalline powder. It is a monoterpene essential oil extracted from various herbs, and the main component of the essential oil of rosemary-pepper (Lippia sidoides), constituting approximately 48% of its composition. This paper presents the behavioral actions of thymol in animal models of anxiety, depression, seizures and sedation, such as elevated plus maze (EPM), open field, rota rod, forced swim, tail suspension, seizure induced by pentylenetetrazol and pentobarbital-induced sleep time. Thymol was administered orally in male mice at doses of 25mg/kg and 50mg / kg. The results showed that thymol at both doses studied did cause muscula relaxament activits change locomotor activity on the test rota rod nor the number of crossings in the open field test, but reducedThe grooming was reduced with the administration of thymol in the open field test. At both doses studied LCE thymol increased all parameters observed suggesting a possible anxiolytic effect. Flumazenil, an antagonist of GABA / benzodiazepine, reversed this effect. In the test of sleep time induced by pentobarbital thymol not alter the latency but increased sleep duration. Although this test is sensitive to CNS depressant agents but is not specific for compounds which would interfere with the biotransformation of pentobarbital. In the test of pentylenetetrazol-induced seizures, thymol increased latency of seizure at the dose of 50mg/kg, however was not able to change the mortality of animals. Thymol showed antidepressant effect in the forced swim test and the tail suspension. This effect was reversed by pretreatment of animals with PCPA (a serotonin synthesis inhibitor), Prazosin and Yohimbine (adrenoreceptor antagonists), SCH23390 and Sulpiride (dopamine receptor antagonists). These results suggest that thymol shows anxiolytic effects probably associated with the GABAergic system and antidepressant effects associated with the serotonergic, noradrenergic and dopaminergic without causing sedation.O Timol (2-isopropil-5-metil-fenol) um isÃmero do Carvacrol. Apresenta-se como cristal incolor grandes ou pà cristalino branco. à um monoterpeno extraÃdo do Ãleo essencial de diversas plantas aromÃticas, sendo o componente principal do Ãleo essencial do alecrim-pimenta (Lippia sidoides), constituindo aproximadamente 48% da sua composiÃÃo. Este trabalho avaliou as aÃÃes comportamentais do timol em modelos animais de ansiedade, depressÃo, convulsÃo e sedaÃÃo, tais como labirinto em cruz elevado (LCE), campo aberto, rota rod, nado forÃado, suspensÃo da cauda, convulsÃo induzida por pentilenotetrazol e tempo de sono induzido por pentobarbital. Timol foi administrado por via oral, em camundongos machos, em doses Ãnicas de 25mg/Kg ou 50 mg/Kg. Os resultados mostraram que o timol nas duas doses estudadas, nÃo causou efeito relaxante muscular no teste do rota Rod nem alterou o nÃmero de cruzamentos no teste do campo aberto, mas reduziu o grooming. No LCE ambas as doses estudadas do timol aumentaram todos os parÃmetros observados sugerindo um possÃvel efeito ansiolÃtico. O flumazenil, um antagonista dos receptores GABAA/BenzodiazepÃnico, reverteu este efeito. No teste do tempo de sono induzido por pentobarbital o timol nÃo alterou a latÃncia, mas aumentou a duraÃÃo do sono. Entretanto este teste embora sendo sensÃvel para agentes depressores do SNC nÃo à especÃfico pois compostos que possam interferir com a biotransformaÃÃo do pentobarbital. No teste da convulsÃo induzida por pentilenotetrazol, timol aumentou a latÃncia da convulsÃo na dose de 50mg/Kg, no entanto nÃo foi capaz de alterar a latÃncia de morte e a mortalidade dos animais. Timol apresentou efeito antidepressivo no teste do nado forÃado e da suspensÃo da cauda. Este efeito foi revertido pelo prÃ-tratamento dos animais com PCPA (um inibidor da sÃntese de serotonina), Prazosina e Ioimbina (antagonistas dos receptores adrenÃrgicos), SCH23390 e Sulpirida (antagonistas dos receptores dopaminÃrgicos). Estes resultados sugerem que o timol apresenta efeitos ansiolÃticos provavelmente relacionados com o sistema GABAÃrgico e efeitos antidepressivos associados aos sistemas serotonÃrgico, noradrenÃrgico e dopaminÃrgico sem causar sedaÃÃo. nÃo hÃhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=10762application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:23:54Zmail@mail.com - |
dc.title.pt.fl_str_mv |
InvestigaÃÃo da aÃÃo central do timol em modelos comportamentais de ansiedade, depressÃo e convulsÃo em camundongos |
title |
InvestigaÃÃo da aÃÃo central do timol em modelos comportamentais de ansiedade, depressÃo e convulsÃo em camundongos |
spellingShingle |
InvestigaÃÃo da aÃÃo central do timol em modelos comportamentais de ansiedade, depressÃo e convulsÃo em camundongos Mariana Lima Fernandes Anxiety, Depression, Thymol FARMACOLOGIA |
title_short |
InvestigaÃÃo da aÃÃo central do timol em modelos comportamentais de ansiedade, depressÃo e convulsÃo em camundongos |
title_full |
InvestigaÃÃo da aÃÃo central do timol em modelos comportamentais de ansiedade, depressÃo e convulsÃo em camundongos |
title_fullStr |
InvestigaÃÃo da aÃÃo central do timol em modelos comportamentais de ansiedade, depressÃo e convulsÃo em camundongos |
title_full_unstemmed |
InvestigaÃÃo da aÃÃo central do timol em modelos comportamentais de ansiedade, depressÃo e convulsÃo em camundongos |
title_sort |
InvestigaÃÃo da aÃÃo central do timol em modelos comportamentais de ansiedade, depressÃo e convulsÃo em camundongos |
author |
Mariana Lima Fernandes |
author_facet |
Mariana Lima Fernandes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Francisca ClÃa FlorenÃo de Sousa |
dc.contributor.advisor1ID.fl_str_mv |
31636020372 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1180465052181572 |
dc.contributor.referee1.fl_str_mv |
Danielle MacÃdo Gaspar |
dc.contributor.referee1ID.fl_str_mv |
50160176387 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1566937332957369 |
dc.contributor.referee2.fl_str_mv |
Gisela Costa CamarÃo |
dc.contributor.referee2ID.fl_str_mv |
06001734372 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/8812515336622893 |
dc.contributor.authorID.fl_str_mv |
80764070363 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6295544741163726 |
dc.contributor.author.fl_str_mv |
Mariana Lima Fernandes |
contributor_str_mv |
Francisca ClÃa FlorenÃo de Sousa Danielle MacÃdo Gaspar Gisela Costa CamarÃo |
dc.subject.eng.fl_str_mv |
Anxiety, Depression, Thymol |
topic |
Anxiety, Depression, Thymol FARMACOLOGIA |
dc.subject.cnpq.fl_str_mv |
FARMACOLOGIA |
dc.description.sponsorship.fl_txt_mv |
nÃo hà |
dc.description.abstract.por.fl_txt_mv |
Thymol (2-isopropyl-5-methyl-phenol) an isomer of Carvacrol. Present as large colorless crystals or white crystalline powder. It is a monoterpene essential oil extracted from various herbs, and the main component of the essential oil of rosemary-pepper (Lippia sidoides), constituting approximately 48% of its composition. This paper presents the behavioral actions of thymol in animal models of anxiety, depression, seizures and sedation, such as elevated plus maze (EPM), open field, rota rod, forced swim, tail suspension, seizure induced by pentylenetetrazol and pentobarbital-induced sleep time. Thymol was administered orally in male mice at doses of 25mg/kg and 50mg / kg. The results showed that thymol at both doses studied did cause muscula relaxament activits change locomotor activity on the test rota rod nor the number of crossings in the open field test, but reducedThe grooming was reduced with the administration of thymol in the open field test. At both doses studied LCE thymol increased all parameters observed suggesting a possible anxiolytic effect. Flumazenil, an antagonist of GABA / benzodiazepine, reversed this effect. In the test of sleep time induced by pentobarbital thymol not alter the latency but increased sleep duration. Although this test is sensitive to CNS depressant agents but is not specific for compounds which would interfere with the biotransformation of pentobarbital. In the test of pentylenetetrazol-induced seizures, thymol increased latency of seizure at the dose of 50mg/kg, however was not able to change the mortality of animals. Thymol showed antidepressant effect in the forced swim test and the tail suspension. This effect was reversed by pretreatment of animals with PCPA (a serotonin synthesis inhibitor), Prazosin and Yohimbine (adrenoreceptor antagonists), SCH23390 and Sulpiride (dopamine receptor antagonists). These results suggest that thymol shows anxiolytic effects probably associated with the GABAergic system and antidepressant effects associated with the serotonergic, noradrenergic and dopaminergic without causing sedation. O Timol (2-isopropil-5-metil-fenol) um isÃmero do Carvacrol. Apresenta-se como cristal incolor grandes ou pà cristalino branco. à um monoterpeno extraÃdo do Ãleo essencial de diversas plantas aromÃticas, sendo o componente principal do Ãleo essencial do alecrim-pimenta (Lippia sidoides), constituindo aproximadamente 48% da sua composiÃÃo. Este trabalho avaliou as aÃÃes comportamentais do timol em modelos animais de ansiedade, depressÃo, convulsÃo e sedaÃÃo, tais como labirinto em cruz elevado (LCE), campo aberto, rota rod, nado forÃado, suspensÃo da cauda, convulsÃo induzida por pentilenotetrazol e tempo de sono induzido por pentobarbital. Timol foi administrado por via oral, em camundongos machos, em doses Ãnicas de 25mg/Kg ou 50 mg/Kg. Os resultados mostraram que o timol nas duas doses estudadas, nÃo causou efeito relaxante muscular no teste do rota Rod nem alterou o nÃmero de cruzamentos no teste do campo aberto, mas reduziu o grooming. No LCE ambas as doses estudadas do timol aumentaram todos os parÃmetros observados sugerindo um possÃvel efeito ansiolÃtico. O flumazenil, um antagonista dos receptores GABAA/BenzodiazepÃnico, reverteu este efeito. No teste do tempo de sono induzido por pentobarbital o timol nÃo alterou a latÃncia, mas aumentou a duraÃÃo do sono. Entretanto este teste embora sendo sensÃvel para agentes depressores do SNC nÃo à especÃfico pois compostos que possam interferir com a biotransformaÃÃo do pentobarbital. No teste da convulsÃo induzida por pentilenotetrazol, timol aumentou a latÃncia da convulsÃo na dose de 50mg/Kg, no entanto nÃo foi capaz de alterar a latÃncia de morte e a mortalidade dos animais. Timol apresentou efeito antidepressivo no teste do nado forÃado e da suspensÃo da cauda. Este efeito foi revertido pelo prÃ-tratamento dos animais com PCPA (um inibidor da sÃntese de serotonina), Prazosina e Ioimbina (antagonistas dos receptores adrenÃrgicos), SCH23390 e Sulpirida (antagonistas dos receptores dopaminÃrgicos). Estes resultados sugerem que o timol apresenta efeitos ansiolÃticos provavelmente relacionados com o sistema GABAÃrgico e efeitos antidepressivos associados aos sistemas serotonÃrgico, noradrenÃrgico e dopaminÃrgico sem causar sedaÃÃo. |
description |
Thymol (2-isopropyl-5-methyl-phenol) an isomer of Carvacrol. Present as large colorless crystals or white crystalline powder. It is a monoterpene essential oil extracted from various herbs, and the main component of the essential oil of rosemary-pepper (Lippia sidoides), constituting approximately 48% of its composition. This paper presents the behavioral actions of thymol in animal models of anxiety, depression, seizures and sedation, such as elevated plus maze (EPM), open field, rota rod, forced swim, tail suspension, seizure induced by pentylenetetrazol and pentobarbital-induced sleep time. Thymol was administered orally in male mice at doses of 25mg/kg and 50mg / kg. The results showed that thymol at both doses studied did cause muscula relaxament activits change locomotor activity on the test rota rod nor the number of crossings in the open field test, but reducedThe grooming was reduced with the administration of thymol in the open field test. At both doses studied LCE thymol increased all parameters observed suggesting a possible anxiolytic effect. Flumazenil, an antagonist of GABA / benzodiazepine, reversed this effect. In the test of sleep time induced by pentobarbital thymol not alter the latency but increased sleep duration. Although this test is sensitive to CNS depressant agents but is not specific for compounds which would interfere with the biotransformation of pentobarbital. In the test of pentylenetetrazol-induced seizures, thymol increased latency of seizure at the dose of 50mg/kg, however was not able to change the mortality of animals. Thymol showed antidepressant effect in the forced swim test and the tail suspension. This effect was reversed by pretreatment of animals with PCPA (a serotonin synthesis inhibitor), Prazosin and Yohimbine (adrenoreceptor antagonists), SCH23390 and Sulpiride (dopamine receptor antagonists). These results suggest that thymol shows anxiolytic effects probably associated with the GABAergic system and antidepressant effects associated with the serotonergic, noradrenergic and dopaminergic without causing sedation. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-11-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
format |
masterThesis |
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http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=10762 |
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http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=10762 |
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por |
language |
por |
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openAccess |
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Universidade Federal do Cearà |
dc.publisher.program.fl_str_mv |
Programa de PÃs-GraduaÃÃo em Farmacologia |
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UFC |
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BR |
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Universidade Federal do Cearà |
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reponame:Biblioteca Digital de Teses e Dissertações da UFC instname:Universidade Federal do Ceará instacron:UFC |
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Biblioteca Digital de Teses e Dissertações da UFC |
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Biblioteca Digital de Teses e Dissertações da UFC |
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Universidade Federal do Ceará |
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UFC |
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UFC |
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