Evaluation of oxidative stress in women diagnosed with primary hypothyroidism
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=19266 |
Resumo: | Thyroid hormones regulate the body metabolism, with changes in cellular oxygen usage profile. As a result, there may be production of reactive oxygen species that induce oxidative stress and cellular dysfunction in various tissues. The study aims to evaluate the levels of oxidative stress in women diagnosed with primary hypothyroidism. The study included 25 female patients from the Endocrinology Clinic of the University Hospital Walter CantÃdio (HUWC) in Fortaleza, CearÃ, with primary hypothyroidism. Blood samples of patients were collected in the hypothyroid condition and euthyroid. We evaluated the thyroid hormone function, biochemical and haematological profile of patients, beyond the parameters of oxidative stress from measurements of malondialdehyde, nitrite/nitrate, superoxide dismutase and catalase. We observed a significant reduction of total cholesterol, HDL, LDL and triglycerides and leukocytes after correction of hypothyroidism. In relation to oxidative stress, there was a significant reduction of catalase activity (50,12 Â12,75 vs 63,77 Â 23,8 atv/min; p = 0.03) when we compared two moments. There was no change in nitrite / nitrate concentration (p = 0.18), malonaldehyde (p = 0.67) and superoxide dismutase activity (p = 0.93) when we analyzed the whole group. There was no significant difference in the concentration of malonaldehyde in both situations (P = 0.67). The concentration of nitrite / nitrate was increased in cases of hypothyroidism with metabolic syndrome (34.07 Â 10.89 Vs 30.12 Â 10.12, p = 0.03). These data suggest that hypothyroidism alters oxidative stress, and catalase activity is the most sensitive marker. When hypothyroidism occurs with metabolic syndrome, NO levels increase, suggesting different mechanisms in oxidative stress in these two clinical conditions. |
id |
UFC_6b04b5bb6a600782f08633c4485a1bd6 |
---|---|
oai_identifier_str |
oai:www.teses.ufc.br:12170 |
network_acronym_str |
UFC |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UFC |
spelling |
info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisEvaluation of oxidative stress in women diagnosed with primary hypothyroidismAvaliaÃÃo do estresse oxidativo em mulheres diagnosticadas com hipotireoidismo primÃrio2017-02-10Manoel Ricardo Alves Martins48034622320http://lattes.cnpq.br/3503117351197846ArlÃndia Cristina Lima Nobre de Morais40410706353http://lattes.cnpq.br/2247671773636111Ana Rosa Pinto Quidute89941357404http://lattes.cnpq.br/0730748142072670Eveline Gadelha Pereira Fontenele4431693335http://lattes.cnpq.br/275119963160817203292151325http://lattes.cnpq.br/3971486501170280LAÃS FARIAS MASULLOUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em PatologiaUFCBRENDOCRINOLOGIAThyroid hormones regulate the body metabolism, with changes in cellular oxygen usage profile. As a result, there may be production of reactive oxygen species that induce oxidative stress and cellular dysfunction in various tissues. The study aims to evaluate the levels of oxidative stress in women diagnosed with primary hypothyroidism. The study included 25 female patients from the Endocrinology Clinic of the University Hospital Walter CantÃdio (HUWC) in Fortaleza, CearÃ, with primary hypothyroidism. Blood samples of patients were collected in the hypothyroid condition and euthyroid. We evaluated the thyroid hormone function, biochemical and haematological profile of patients, beyond the parameters of oxidative stress from measurements of malondialdehyde, nitrite/nitrate, superoxide dismutase and catalase. We observed a significant reduction of total cholesterol, HDL, LDL and triglycerides and leukocytes after correction of hypothyroidism. In relation to oxidative stress, there was a significant reduction of catalase activity (50,12 Â12,75 vs 63,77  23,8 atv/min; p = 0.03) when we compared two moments. There was no change in nitrite / nitrate concentration (p = 0.18), malonaldehyde (p = 0.67) and superoxide dismutase activity (p = 0.93) when we analyzed the whole group. There was no significant difference in the concentration of malonaldehyde in both situations (P = 0.67). The concentration of nitrite / nitrate was increased in cases of hypothyroidism with metabolic syndrome (34.07  10.89 Vs 30.12  10.12, p = 0.03). These data suggest that hypothyroidism alters oxidative stress, and catalase activity is the most sensitive marker. When hypothyroidism occurs with metabolic syndrome, NO levels increase, suggesting different mechanisms in oxidative stress in these two clinical conditions. Os hormÃnios tireoidianos regulam o metabolismo corporal, com mudanÃa no perfil de utilizaÃÃo de oxigÃnio celular. Como consequÃncia, pode ocorrer a produÃÃo de espÃcies reativas de oxigÃnio que induzem o estresse oxidativo e disfunÃÃes celulares em diversos tecidos. O estudo tem como objetivo avaliar os nÃveis de estresse oxidativo em mulheres diagnosticadas com hipotireoidismo primÃrio. Participaram do estudo 25 pacientes do sexo feminino, atendidas no ambulatÃrio de Endocrinologia do Hospital UniversitÃrio Walter CantÃdio (HUWC) em Fortaleza-CearÃ, com hipotireoidismo primÃrio. As amostras de sangue dos pacientes foram coletadas na condiÃÃo de hipotireoidismo e em eutireoidismo. Avaliou-se a funÃÃo hormonal tireoideana, o perfil bioquÃmico e hematolÃgico dos pacientes, alÃm dos parÃmetros do estresse oxidativo a partir das dosagens de malonaldeÃdo, nitrito/nitrato, catalase e superÃxido dismutase. Observamos reduÃÃo significativa do colesterol total, LDL e triglicÃrideos apÃs a correÃÃo do hipotireoidismo. Em relaÃÃo ao estresse oxidativo, houve aumento da atividade da catalase (50,25  12,75 vs 63,77  23,8 atv/min; p = 0,03) quando comparamos os dois momentos. NÃo houve alteraÃÃo no nitrito/nitrato (p=0,18), malonaldeÃdo (p=0,67) e na atividade da superÃxido dismutase (p=0,93) quando analisamos o grupo inteiro. A concentraÃÃo de nitrito/nitrato estava aumentada nos casos de hipotireoidismo com sÃndrome metabÃlica (34,07  10,89 vs 30,12 Â10,12; p=0,03). Esses dados sugerem que o hipotireoidismo altera o estresse oxidativo, sendo que a atividade da catalase à o marcador mais sensÃvel. Quando o hipotireoidismo està associado à sÃndrome metabÃlica, os nÃveis de NO aumentam, o que sugere mecanismos diferentes no estresse oxidativo nessas duas condiÃÃes clÃnicas.CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superiorhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=19266application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:31:53Zmail@mail.com - |
dc.title.en.fl_str_mv |
Evaluation of oxidative stress in women diagnosed with primary hypothyroidism |
dc.title.alternative.pt.fl_str_mv |
AvaliaÃÃo do estresse oxidativo em mulheres diagnosticadas com hipotireoidismo primÃrio |
title |
Evaluation of oxidative stress in women diagnosed with primary hypothyroidism |
spellingShingle |
Evaluation of oxidative stress in women diagnosed with primary hypothyroidism LAÃS FARIAS MASULLO ENDOCRINOLOGIA |
title_short |
Evaluation of oxidative stress in women diagnosed with primary hypothyroidism |
title_full |
Evaluation of oxidative stress in women diagnosed with primary hypothyroidism |
title_fullStr |
Evaluation of oxidative stress in women diagnosed with primary hypothyroidism |
title_full_unstemmed |
Evaluation of oxidative stress in women diagnosed with primary hypothyroidism |
title_sort |
Evaluation of oxidative stress in women diagnosed with primary hypothyroidism |
author |
LAÃS FARIAS MASULLO |
author_facet |
LAÃS FARIAS MASULLO |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Manoel Ricardo Alves Martins |
dc.contributor.advisor1ID.fl_str_mv |
48034622320 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3503117351197846 |
dc.contributor.referee1.fl_str_mv |
ArlÃndia Cristina Lima Nobre de Morais |
dc.contributor.referee1ID.fl_str_mv |
40410706353 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2247671773636111 |
dc.contributor.referee2.fl_str_mv |
Ana Rosa Pinto Quidute |
dc.contributor.referee2ID.fl_str_mv |
89941357404 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/0730748142072670 |
dc.contributor.referee3.fl_str_mv |
Eveline Gadelha Pereira Fontenele |
dc.contributor.referee3ID.fl_str_mv |
4431693335 |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/2751199631608172 |
dc.contributor.authorID.fl_str_mv |
03292151325 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3971486501170280 |
dc.contributor.author.fl_str_mv |
LAÃS FARIAS MASULLO |
contributor_str_mv |
Manoel Ricardo Alves Martins ArlÃndia Cristina Lima Nobre de Morais Ana Rosa Pinto Quidute Eveline Gadelha Pereira Fontenele |
dc.subject.cnpq.fl_str_mv |
ENDOCRINOLOGIA |
topic |
ENDOCRINOLOGIA |
dc.description.sponsorship.fl_txt_mv |
CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior |
dc.description.abstract.por.fl_txt_mv |
Thyroid hormones regulate the body metabolism, with changes in cellular oxygen usage profile. As a result, there may be production of reactive oxygen species that induce oxidative stress and cellular dysfunction in various tissues. The study aims to evaluate the levels of oxidative stress in women diagnosed with primary hypothyroidism. The study included 25 female patients from the Endocrinology Clinic of the University Hospital Walter CantÃdio (HUWC) in Fortaleza, CearÃ, with primary hypothyroidism. Blood samples of patients were collected in the hypothyroid condition and euthyroid. We evaluated the thyroid hormone function, biochemical and haematological profile of patients, beyond the parameters of oxidative stress from measurements of malondialdehyde, nitrite/nitrate, superoxide dismutase and catalase. We observed a significant reduction of total cholesterol, HDL, LDL and triglycerides and leukocytes after correction of hypothyroidism. In relation to oxidative stress, there was a significant reduction of catalase activity (50,12 Â12,75 vs 63,77  23,8 atv/min; p = 0.03) when we compared two moments. There was no change in nitrite / nitrate concentration (p = 0.18), malonaldehyde (p = 0.67) and superoxide dismutase activity (p = 0.93) when we analyzed the whole group. There was no significant difference in the concentration of malonaldehyde in both situations (P = 0.67). The concentration of nitrite / nitrate was increased in cases of hypothyroidism with metabolic syndrome (34.07  10.89 Vs 30.12  10.12, p = 0.03). These data suggest that hypothyroidism alters oxidative stress, and catalase activity is the most sensitive marker. When hypothyroidism occurs with metabolic syndrome, NO levels increase, suggesting different mechanisms in oxidative stress in these two clinical conditions. Os hormÃnios tireoidianos regulam o metabolismo corporal, com mudanÃa no perfil de utilizaÃÃo de oxigÃnio celular. Como consequÃncia, pode ocorrer a produÃÃo de espÃcies reativas de oxigÃnio que induzem o estresse oxidativo e disfunÃÃes celulares em diversos tecidos. O estudo tem como objetivo avaliar os nÃveis de estresse oxidativo em mulheres diagnosticadas com hipotireoidismo primÃrio. Participaram do estudo 25 pacientes do sexo feminino, atendidas no ambulatÃrio de Endocrinologia do Hospital UniversitÃrio Walter CantÃdio (HUWC) em Fortaleza-CearÃ, com hipotireoidismo primÃrio. As amostras de sangue dos pacientes foram coletadas na condiÃÃo de hipotireoidismo e em eutireoidismo. Avaliou-se a funÃÃo hormonal tireoideana, o perfil bioquÃmico e hematolÃgico dos pacientes, alÃm dos parÃmetros do estresse oxidativo a partir das dosagens de malonaldeÃdo, nitrito/nitrato, catalase e superÃxido dismutase. Observamos reduÃÃo significativa do colesterol total, LDL e triglicÃrideos apÃs a correÃÃo do hipotireoidismo. Em relaÃÃo ao estresse oxidativo, houve aumento da atividade da catalase (50,25  12,75 vs 63,77  23,8 atv/min; p = 0,03) quando comparamos os dois momentos. NÃo houve alteraÃÃo no nitrito/nitrato (p=0,18), malonaldeÃdo (p=0,67) e na atividade da superÃxido dismutase (p=0,93) quando analisamos o grupo inteiro. A concentraÃÃo de nitrito/nitrato estava aumentada nos casos de hipotireoidismo com sÃndrome metabÃlica (34,07  10,89 vs 30,12 Â10,12; p=0,03). Esses dados sugerem que o hipotireoidismo altera o estresse oxidativo, sendo que a atividade da catalase à o marcador mais sensÃvel. Quando o hipotireoidismo està associado à sÃndrome metabÃlica, os nÃveis de NO aumentam, o que sugere mecanismos diferentes no estresse oxidativo nessas duas condiÃÃes clÃnicas. |
description |
Thyroid hormones regulate the body metabolism, with changes in cellular oxygen usage profile. As a result, there may be production of reactive oxygen species that induce oxidative stress and cellular dysfunction in various tissues. The study aims to evaluate the levels of oxidative stress in women diagnosed with primary hypothyroidism. The study included 25 female patients from the Endocrinology Clinic of the University Hospital Walter CantÃdio (HUWC) in Fortaleza, CearÃ, with primary hypothyroidism. Blood samples of patients were collected in the hypothyroid condition and euthyroid. We evaluated the thyroid hormone function, biochemical and haematological profile of patients, beyond the parameters of oxidative stress from measurements of malondialdehyde, nitrite/nitrate, superoxide dismutase and catalase. We observed a significant reduction of total cholesterol, HDL, LDL and triglycerides and leukocytes after correction of hypothyroidism. In relation to oxidative stress, there was a significant reduction of catalase activity (50,12 Â12,75 vs 63,77 Â 23,8 atv/min; p = 0.03) when we compared two moments. There was no change in nitrite / nitrate concentration (p = 0.18), malonaldehyde (p = 0.67) and superoxide dismutase activity (p = 0.93) when we analyzed the whole group. There was no significant difference in the concentration of malonaldehyde in both situations (P = 0.67). The concentration of nitrite / nitrate was increased in cases of hypothyroidism with metabolic syndrome (34.07 Â 10.89 Vs 30.12 Â 10.12, p = 0.03). These data suggest that hypothyroidism alters oxidative stress, and catalase activity is the most sensitive marker. When hypothyroidism occurs with metabolic syndrome, NO levels increase, suggesting different mechanisms in oxidative stress in these two clinical conditions. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-02-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
status_str |
publishedVersion |
format |
masterThesis |
dc.identifier.uri.fl_str_mv |
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=19266 |
url |
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=19266 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Cearà |
dc.publisher.program.fl_str_mv |
Programa de PÃs-GraduaÃÃo em Patologia |
dc.publisher.initials.fl_str_mv |
UFC |
dc.publisher.country.fl_str_mv |
BR |
publisher.none.fl_str_mv |
Universidade Federal do Cearà |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFC instname:Universidade Federal do Ceará instacron:UFC |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFC |
collection |
Biblioteca Digital de Teses e Dissertações da UFC |
instname_str |
Universidade Federal do Ceará |
instacron_str |
UFC |
institution |
UFC |
repository.name.fl_str_mv |
-
|
repository.mail.fl_str_mv |
mail@mail.com |
_version_ |
1643295233356595200 |