Susceptibility the antimicrobial and profile of strength in strains plasmid Escherichia coli isolated freshwater fish and marine, marketed in Fortaleza - Ce.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16332 |
Resumo: | The marketing of fish has shown substantial increase; however, their place of origin and ways of handling and storage may pose health risk. The microorganisms in the food chain and in capture environments cause concern over the risk of disease transmission and the multiple resistance to several drugs. This study aimed at characterizing the antimicrobial resistance of 191 strains of Escherichia coli isolated from 20 fish samples, ten saltwater samples: (5) mackerel (S. cavalla) and (5) snapper (L. purpureus) and ten freshwater samples: (5) curimatà (P. cearenses) and (5) tilapia (O. niloticus), sold in street markets of the city of Fortaleza (Cearà State), Brazil. The strains were isolated, identified and then subjected to susceptibility testing (20 antibiotics) in order to provide the resistance profile. Moreover, it was also performed: Multiple Resistance Index (MRI), the Antimicrobial Resistance Index (ARI), Minimum Inhibitory Concentration (MIC), plasmid "cure" and analysis of plasmid profile through DNA extraction. Strains isolated from marine and freshwater fish species showed distinct multidrug resistance profiles, but with broad resistance to penicillins and tetracyclines. There was high frequency of saltwater isolates resistant to chloramphenicol. Overall, low resistance to betalactamase inhibitors (ampicillin/sulbactam, and piperacillin/tazobactam) and aminoglycosides was observed, excepting streptomycin. The MRI has shown that about 50% of the isolates were resistant to five of the twenty tested antimicrobials, highlighting the strains from mackerel (55.5%). As for MIC, the percentage of isolates resistant to NAL, CIP, MFX, SUT and AMO stood out in saltwater fish strains. For bacteria from freshwater, greater resistance to high concentrations of antimicrobials were most significant for AMO, SUL and NAL. Chromosomal profiles in freshwater isolates were mostly composed of amoxicillin, ampicillin, streptomycin, and sulfametazol/trimethoprim. For saltwater fish, there was predominance of quinolones. Analysis of the plasmid DNA showed the occurrence of a heterogeneous population of small plasmids distributed in various profiles. The greater diversity and lower molecular weight were observed in strains of marine fish samples. Thus, it is emphasized that the circulation of pathogenic E. coli with antimicrobial resistance characteristics poses a risk to the aquatic ecosystem and the marketing environment, there being need for continued vigilance to contaminant bacterial to fish so that the safety of these foods is guaranteed. |
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Biblioteca Digital de Teses e Dissertações da UFC |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisSusceptibility the antimicrobial and profile of strength in strains plasmid Escherichia coli isolated freshwater fish and marine, marketed in Fortaleza - Ce.Susceptibilidade a antimicrobianos e perfil de resistÃncia plasmidial em cepas de Escherichia coli isoladas de pescado de Ãgua doce e marinha, comercializados em feiras de Fortaleza-Ce2015-11-05Regine Helena Silva dos Fernandes Vieira09061029368Oscarina Viana de Sousa38583933391http://lattes.cnpq.br/6529999796909142Elisabeth Mary Cunha da Silva24392111334http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4793261Y0Suzana ClÃudia Silveira Martins11977558372 http://lattes.cnpq.br/8340687824475620 MARILIA OETTERER 0170809986700198079370http://lattes.cnpq.br/4933039592606571Adalva Lopes MachadoUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em Engenharia de PescaUFCBRAntibiÃticoBactÃrias resistentesContaminaÃÃoCavalaPargoCurimatÃAntibiotic Resistant bacteriaResistant bacteriaContaminationMackerelSnapperTilapiaRECURSOS PESQUEIROS E ENGENHARIA DE PESCAThe marketing of fish has shown substantial increase; however, their place of origin and ways of handling and storage may pose health risk. The microorganisms in the food chain and in capture environments cause concern over the risk of disease transmission and the multiple resistance to several drugs. This study aimed at characterizing the antimicrobial resistance of 191 strains of Escherichia coli isolated from 20 fish samples, ten saltwater samples: (5) mackerel (S. cavalla) and (5) snapper (L. purpureus) and ten freshwater samples: (5) curimatà (P. cearenses) and (5) tilapia (O. niloticus), sold in street markets of the city of Fortaleza (Cearà State), Brazil. The strains were isolated, identified and then subjected to susceptibility testing (20 antibiotics) in order to provide the resistance profile. Moreover, it was also performed: Multiple Resistance Index (MRI), the Antimicrobial Resistance Index (ARI), Minimum Inhibitory Concentration (MIC), plasmid "cure" and analysis of plasmid profile through DNA extraction. Strains isolated from marine and freshwater fish species showed distinct multidrug resistance profiles, but with broad resistance to penicillins and tetracyclines. There was high frequency of saltwater isolates resistant to chloramphenicol. Overall, low resistance to betalactamase inhibitors (ampicillin/sulbactam, and piperacillin/tazobactam) and aminoglycosides was observed, excepting streptomycin. The MRI has shown that about 50% of the isolates were resistant to five of the twenty tested antimicrobials, highlighting the strains from mackerel (55.5%). As for MIC, the percentage of isolates resistant to NAL, CIP, MFX, SUT and AMO stood out in saltwater fish strains. For bacteria from freshwater, greater resistance to high concentrations of antimicrobials were most significant for AMO, SUL and NAL. Chromosomal profiles in freshwater isolates were mostly composed of amoxicillin, ampicillin, streptomycin, and sulfametazol/trimethoprim. For saltwater fish, there was predominance of quinolones. Analysis of the plasmid DNA showed the occurrence of a heterogeneous population of small plasmids distributed in various profiles. The greater diversity and lower molecular weight were observed in strains of marine fish samples. Thus, it is emphasized that the circulation of pathogenic E. coli with antimicrobial resistance characteristics poses a risk to the aquatic ecosystem and the marketing environment, there being need for continued vigilance to contaminant bacterial to fish so that the safety of these foods is guaranteed. A comercializaÃÃo de pescado tem indicado aumento substancial, contudo, seu local de origem e formas de manipulaÃÃo e armazenamento, podem representar risco sanitÃrio. Os micro-organismos presentes na cadeia produtiva de alimentos e nos ambientes de captura ocasionam preocupaÃÃo pelo risco de transmissÃo de doenÃas e pela mÃltipla resistÃncia apresentada a diversos fÃrmacos. Este trabalho objetivou caracterizar a resistÃncia antimicrobiana de 191 cepas de Escherichia coli isoladas de 20 amostras de pescado, sendo dez amostras de pescado de Ãgua salgada: (5) cavala (S. cavalla) e (5) pargo (L. purpureus) e dez amostras de Ãgua doce: (5) curimatà (P. cearenses) e (5) tilÃpia (O.niloticus), comercializadas em feiras livres da cidade de Fortaleza, CE. As cepas foram isoladas, identificadas e posteriormente submetidas a testes de susceptibilidade (20 antimicrobianos) a fim de conferir o perfil de resistÃncia. AlÃm disso, foram realizados: Ãndice de MÃltipla ResistÃncia (IMR), Ãndice de ResistÃncia a Antimicrobianos (IRA), ConcentraÃÃo InibitÃria MÃnina (CIM), âcuraâ plasmidial e anÃlise de perfil plasmidial, atravÃs de extraÃÃo de DNA. As cepas isoladas das espÃcies de pescado marinho e de Ãgua doce apresentaram perfis de multirresistÃncia distintos, porÃm com ampla resistÃncia Ãs penicilinas e Ãs tetraciclinas. Houve elevada frequÃncia de isolados de pescado marinho resistentes ao cloranfenicol. Foi observada, de forma geral, baixa resistÃncia para inibidores de betalactamases (ampicilina/ sulbactam e piperacilina/ tazobactam) e aminoglicosÃdeos, excetuando, estreptomicina. O IMR demonstrou que cerca de 50% dos isolados foram resistentes a cinco dos vinte antimicrobianos testados, destacando as cepas provenientes de cavala (55,5%). Nos testes de CIM observou-se que, cepas isoladas de pescados de Ãgua salgada apresentaram maiores percentuais de isolados resistentes a NAL, CIP, MFX, SUT e AMO. Maiores resistÃncias a elevadas concentraÃÃes de antimicrobianos, quando testadas bactÃrias oriundas de Ãgua doce, foram observadas para AMO, SUL e NAL. Os perfis cromossomiais em isolados de Ãgua doce foram na maioria compostos por amoxicilina, ampicilina, estreptomicina e sulfametazol ∕ trimetropim. Para o pescado de Ãgua salgada, foi verificado a predominÃncia de quinolonas. A anÃlise do DNA plasmidial mostrou a ocorrÃncia de uma populaÃÃo heterogÃnea de pequenos plasmÃdios distribuÃdos em vÃrios perfis. A maior diversidade e menor peso molecular foram observados em cepas de amostras de pescado de origem marinha. Dessa forma, destaca-se que a circulaÃÃo de E. coli patogÃnicas com caracterÃsticas de resistÃncia antimicrobiana representa um risco ao ecossistema aquÃtico e ao ambiente de comercializaÃÃo, havendo necessidade de vigilÃncia contÃnua a bactÃrias contaminantes do pescado, para que a seguranÃa desses alimentos seja garantida.CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16332application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:29:50Zmail@mail.com - |
dc.title.en.fl_str_mv |
Susceptibility the antimicrobial and profile of strength in strains plasmid Escherichia coli isolated freshwater fish and marine, marketed in Fortaleza - Ce. |
dc.title.alternative.pt.fl_str_mv |
Susceptibilidade a antimicrobianos e perfil de resistÃncia plasmidial em cepas de Escherichia coli isoladas de pescado de Ãgua doce e marinha, comercializados em feiras de Fortaleza-Ce |
title |
Susceptibility the antimicrobial and profile of strength in strains plasmid Escherichia coli isolated freshwater fish and marine, marketed in Fortaleza - Ce. |
spellingShingle |
Susceptibility the antimicrobial and profile of strength in strains plasmid Escherichia coli isolated freshwater fish and marine, marketed in Fortaleza - Ce. Adalva Lopes Machado AntibiÃtico BactÃrias resistentes ContaminaÃÃo Cavala Pargo Curimatà Antibiotic Resistant bacteria Resistant bacteria Contamination Mackerel Snapper Tilapia RECURSOS PESQUEIROS E ENGENHARIA DE PESCA |
title_short |
Susceptibility the antimicrobial and profile of strength in strains plasmid Escherichia coli isolated freshwater fish and marine, marketed in Fortaleza - Ce. |
title_full |
Susceptibility the antimicrobial and profile of strength in strains plasmid Escherichia coli isolated freshwater fish and marine, marketed in Fortaleza - Ce. |
title_fullStr |
Susceptibility the antimicrobial and profile of strength in strains plasmid Escherichia coli isolated freshwater fish and marine, marketed in Fortaleza - Ce. |
title_full_unstemmed |
Susceptibility the antimicrobial and profile of strength in strains plasmid Escherichia coli isolated freshwater fish and marine, marketed in Fortaleza - Ce. |
title_sort |
Susceptibility the antimicrobial and profile of strength in strains plasmid Escherichia coli isolated freshwater fish and marine, marketed in Fortaleza - Ce. |
author |
Adalva Lopes Machado |
author_facet |
Adalva Lopes Machado |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Regine Helena Silva dos Fernandes Vieira |
dc.contributor.advisor1ID.fl_str_mv |
09061029368 |
dc.contributor.referee1.fl_str_mv |
Oscarina Viana de Sousa |
dc.contributor.referee1ID.fl_str_mv |
38583933391 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6529999796909142 |
dc.contributor.referee2.fl_str_mv |
Elisabeth Mary Cunha da Silva |
dc.contributor.referee2ID.fl_str_mv |
24392111334 |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4793261Y0 |
dc.contributor.referee3.fl_str_mv |
Suzana ClÃudia Silveira Martins |
dc.contributor.referee3ID.fl_str_mv |
11977558372 |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/8340687824475620 |
dc.contributor.referee4.fl_str_mv |
MARILIA OETTERER |
dc.contributor.referee4ID.fl_str_mv |
01708099867 |
dc.contributor.authorID.fl_str_mv |
00198079370 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/4933039592606571 |
dc.contributor.author.fl_str_mv |
Adalva Lopes Machado |
contributor_str_mv |
Regine Helena Silva dos Fernandes Vieira Oscarina Viana de Sousa Elisabeth Mary Cunha da Silva Suzana ClÃudia Silveira Martins MARILIA OETTERER |
dc.subject.por.fl_str_mv |
AntibiÃtico BactÃrias resistentes ContaminaÃÃo Cavala Pargo Curimatà |
topic |
AntibiÃtico BactÃrias resistentes ContaminaÃÃo Cavala Pargo Curimatà Antibiotic Resistant bacteria Resistant bacteria Contamination Mackerel Snapper Tilapia RECURSOS PESQUEIROS E ENGENHARIA DE PESCA |
dc.subject.eng.fl_str_mv |
Antibiotic Resistant bacteria Resistant bacteria Contamination Mackerel Snapper Tilapia |
dc.subject.cnpq.fl_str_mv |
RECURSOS PESQUEIROS E ENGENHARIA DE PESCA |
dc.description.sponsorship.fl_txt_mv |
CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior |
dc.description.abstract.por.fl_txt_mv |
The marketing of fish has shown substantial increase; however, their place of origin and ways of handling and storage may pose health risk. The microorganisms in the food chain and in capture environments cause concern over the risk of disease transmission and the multiple resistance to several drugs. This study aimed at characterizing the antimicrobial resistance of 191 strains of Escherichia coli isolated from 20 fish samples, ten saltwater samples: (5) mackerel (S. cavalla) and (5) snapper (L. purpureus) and ten freshwater samples: (5) curimatà (P. cearenses) and (5) tilapia (O. niloticus), sold in street markets of the city of Fortaleza (Cearà State), Brazil. The strains were isolated, identified and then subjected to susceptibility testing (20 antibiotics) in order to provide the resistance profile. Moreover, it was also performed: Multiple Resistance Index (MRI), the Antimicrobial Resistance Index (ARI), Minimum Inhibitory Concentration (MIC), plasmid "cure" and analysis of plasmid profile through DNA extraction. Strains isolated from marine and freshwater fish species showed distinct multidrug resistance profiles, but with broad resistance to penicillins and tetracyclines. There was high frequency of saltwater isolates resistant to chloramphenicol. Overall, low resistance to betalactamase inhibitors (ampicillin/sulbactam, and piperacillin/tazobactam) and aminoglycosides was observed, excepting streptomycin. The MRI has shown that about 50% of the isolates were resistant to five of the twenty tested antimicrobials, highlighting the strains from mackerel (55.5%). As for MIC, the percentage of isolates resistant to NAL, CIP, MFX, SUT and AMO stood out in saltwater fish strains. For bacteria from freshwater, greater resistance to high concentrations of antimicrobials were most significant for AMO, SUL and NAL. Chromosomal profiles in freshwater isolates were mostly composed of amoxicillin, ampicillin, streptomycin, and sulfametazol/trimethoprim. For saltwater fish, there was predominance of quinolones. Analysis of the plasmid DNA showed the occurrence of a heterogeneous population of small plasmids distributed in various profiles. The greater diversity and lower molecular weight were observed in strains of marine fish samples. Thus, it is emphasized that the circulation of pathogenic E. coli with antimicrobial resistance characteristics poses a risk to the aquatic ecosystem and the marketing environment, there being need for continued vigilance to contaminant bacterial to fish so that the safety of these foods is guaranteed. A comercializaÃÃo de pescado tem indicado aumento substancial, contudo, seu local de origem e formas de manipulaÃÃo e armazenamento, podem representar risco sanitÃrio. Os micro-organismos presentes na cadeia produtiva de alimentos e nos ambientes de captura ocasionam preocupaÃÃo pelo risco de transmissÃo de doenÃas e pela mÃltipla resistÃncia apresentada a diversos fÃrmacos. Este trabalho objetivou caracterizar a resistÃncia antimicrobiana de 191 cepas de Escherichia coli isoladas de 20 amostras de pescado, sendo dez amostras de pescado de Ãgua salgada: (5) cavala (S. cavalla) e (5) pargo (L. purpureus) e dez amostras de Ãgua doce: (5) curimatà (P. cearenses) e (5) tilÃpia (O.niloticus), comercializadas em feiras livres da cidade de Fortaleza, CE. As cepas foram isoladas, identificadas e posteriormente submetidas a testes de susceptibilidade (20 antimicrobianos) a fim de conferir o perfil de resistÃncia. AlÃm disso, foram realizados: Ãndice de MÃltipla ResistÃncia (IMR), Ãndice de ResistÃncia a Antimicrobianos (IRA), ConcentraÃÃo InibitÃria MÃnina (CIM), âcuraâ plasmidial e anÃlise de perfil plasmidial, atravÃs de extraÃÃo de DNA. As cepas isoladas das espÃcies de pescado marinho e de Ãgua doce apresentaram perfis de multirresistÃncia distintos, porÃm com ampla resistÃncia Ãs penicilinas e Ãs tetraciclinas. Houve elevada frequÃncia de isolados de pescado marinho resistentes ao cloranfenicol. Foi observada, de forma geral, baixa resistÃncia para inibidores de betalactamases (ampicilina/ sulbactam e piperacilina/ tazobactam) e aminoglicosÃdeos, excetuando, estreptomicina. O IMR demonstrou que cerca de 50% dos isolados foram resistentes a cinco dos vinte antimicrobianos testados, destacando as cepas provenientes de cavala (55,5%). Nos testes de CIM observou-se que, cepas isoladas de pescados de Ãgua salgada apresentaram maiores percentuais de isolados resistentes a NAL, CIP, MFX, SUT e AMO. Maiores resistÃncias a elevadas concentraÃÃes de antimicrobianos, quando testadas bactÃrias oriundas de Ãgua doce, foram observadas para AMO, SUL e NAL. Os perfis cromossomiais em isolados de Ãgua doce foram na maioria compostos por amoxicilina, ampicilina, estreptomicina e sulfametazol ∕ trimetropim. Para o pescado de Ãgua salgada, foi verificado a predominÃncia de quinolonas. A anÃlise do DNA plasmidial mostrou a ocorrÃncia de uma populaÃÃo heterogÃnea de pequenos plasmÃdios distribuÃdos em vÃrios perfis. A maior diversidade e menor peso molecular foram observados em cepas de amostras de pescado de origem marinha. Dessa forma, destaca-se que a circulaÃÃo de E. coli patogÃnicas com caracterÃsticas de resistÃncia antimicrobiana representa um risco ao ecossistema aquÃtico e ao ambiente de comercializaÃÃo, havendo necessidade de vigilÃncia contÃnua a bactÃrias contaminantes do pescado, para que a seguranÃa desses alimentos seja garantida. |
description |
The marketing of fish has shown substantial increase; however, their place of origin and ways of handling and storage may pose health risk. The microorganisms in the food chain and in capture environments cause concern over the risk of disease transmission and the multiple resistance to several drugs. This study aimed at characterizing the antimicrobial resistance of 191 strains of Escherichia coli isolated from 20 fish samples, ten saltwater samples: (5) mackerel (S. cavalla) and (5) snapper (L. purpureus) and ten freshwater samples: (5) curimatà (P. cearenses) and (5) tilapia (O. niloticus), sold in street markets of the city of Fortaleza (Cearà State), Brazil. The strains were isolated, identified and then subjected to susceptibility testing (20 antibiotics) in order to provide the resistance profile. Moreover, it was also performed: Multiple Resistance Index (MRI), the Antimicrobial Resistance Index (ARI), Minimum Inhibitory Concentration (MIC), plasmid "cure" and analysis of plasmid profile through DNA extraction. Strains isolated from marine and freshwater fish species showed distinct multidrug resistance profiles, but with broad resistance to penicillins and tetracyclines. There was high frequency of saltwater isolates resistant to chloramphenicol. Overall, low resistance to betalactamase inhibitors (ampicillin/sulbactam, and piperacillin/tazobactam) and aminoglycosides was observed, excepting streptomycin. The MRI has shown that about 50% of the isolates were resistant to five of the twenty tested antimicrobials, highlighting the strains from mackerel (55.5%). As for MIC, the percentage of isolates resistant to NAL, CIP, MFX, SUT and AMO stood out in saltwater fish strains. For bacteria from freshwater, greater resistance to high concentrations of antimicrobials were most significant for AMO, SUL and NAL. Chromosomal profiles in freshwater isolates were mostly composed of amoxicillin, ampicillin, streptomycin, and sulfametazol/trimethoprim. For saltwater fish, there was predominance of quinolones. Analysis of the plasmid DNA showed the occurrence of a heterogeneous population of small plasmids distributed in various profiles. The greater diversity and lower molecular weight were observed in strains of marine fish samples. Thus, it is emphasized that the circulation of pathogenic E. coli with antimicrobial resistance characteristics poses a risk to the aquatic ecosystem and the marketing environment, there being need for continued vigilance to contaminant bacterial to fish so that the safety of these foods is guaranteed. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-11-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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publishedVersion |
format |
doctoralThesis |
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http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16332 |
url |
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16332 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Cearà |
dc.publisher.program.fl_str_mv |
Programa de PÃs-GraduaÃÃo em Engenharia de Pesca |
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UFC |
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BR |
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Universidade Federal do Cearà |
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Universidade Federal do Ceará |
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UFC |
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