Collagen - sulfated polysaccharide films with antithrombogenic properties: preparation and characterization

Detalhes bibliográficos
Autor(a) principal: Ana AngÃlica Mathias Macedo
Data de Publicação: 2006
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7709
Resumo: This work reports the preparation and characterization of collagen-sulfated polysaccharide films and applications in coating of cardiovascular prostheses. The films were prepared by adding the sulfated polysaccharide solution on soluble collagen (0, 5, 10, 20 and 25 %). The blends of collagen-sulfated polysaccharide were casted in acrylic molds, and dried at low temperature. The physico-chemical properties of collagen-sulfated polysaccharide films, were studied using infrared spectroscopy that showed absorptions typical of collagen and sulphated polysaccharide; the thermal analysis (DSC) showed that the structural integrity of collagen was unmodified during the extraction process and the polysaccharide present in the samples did not decrease the thermal stability of the collagen, which was higher for the concentrations of 20 %. In the thermal analysis (DTG), the addition of the polysaccharide caused a small decrease of the stability of thermal degradation and when was adding 5 % of the sulfated polysaccharide to the collagen, a reduction in its stability occurs, however, in the concentrations of 10, 20 and 25 %, the stability of thermal degradation of the film increased. In the impedance spectroscopy, the results are associated possibly, to the interactions between the macromolecules. The blood compatibility was analyzed by platelet adhesion and the results showed that the films possess hemocompatibility and antitrombogenic properties.
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spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisCollagen - sulfated polysaccharide films with antithrombogenic properties: preparation and characterization Filmes de colÃgeno - polissacarÃdeo sulfatado como matrizes com propriedades antitrombogÃnicas: preparaÃÃo e caracterizaÃÃo 2006-03-03Renato de Azevedo Moreira00114715300http://lattes.cnpq.br/4451208231291916SÃnia Duarte FigueirÃ11258470349http://lattes.cnpq.br/2412379131379664Andrà Luis Coelho da Silva625 499 953 68http://lattes.cnpq.br/3701975151596050 35853280325http://lattes.cnpq.br/7219178586824787Ana AngÃlica Mathias MacedoUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em BioquÃmicaUFCBRColÃgeno PolissacarÃdeo sulfatado PrÃteses vasculares Matrizes heparinizadascollagen sulfated polysaccharidecardiovascular prosthesesQUIMICA DOS PRODUTOS NATURAISThis work reports the preparation and characterization of collagen-sulfated polysaccharide films and applications in coating of cardiovascular prostheses. The films were prepared by adding the sulfated polysaccharide solution on soluble collagen (0, 5, 10, 20 and 25 %). The blends of collagen-sulfated polysaccharide were casted in acrylic molds, and dried at low temperature. The physico-chemical properties of collagen-sulfated polysaccharide films, were studied using infrared spectroscopy that showed absorptions typical of collagen and sulphated polysaccharide; the thermal analysis (DSC) showed that the structural integrity of collagen was unmodified during the extraction process and the polysaccharide present in the samples did not decrease the thermal stability of the collagen, which was higher for the concentrations of 20 %. In the thermal analysis (DTG), the addition of the polysaccharide caused a small decrease of the stability of thermal degradation and when was adding 5 % of the sulfated polysaccharide to the collagen, a reduction in its stability occurs, however, in the concentrations of 10, 20 and 25 %, the stability of thermal degradation of the film increased. In the impedance spectroscopy, the results are associated possibly, to the interactions between the macromolecules. The blood compatibility was analyzed by platelet adhesion and the results showed that the films possess hemocompatibility and antitrombogenic properties.Este trabalho aborda sobre o preparo e a caracterizaÃÃo de filmes de colÃgeno-polissacarÃdeo sulfatado e sua aplicaÃÃo para revestimento de prÃteses vasculares. Os filmes foram obtidos a partir da mistura de soluÃÃo de colÃgeno e polissacarÃdeo sulfatado, nas concentraÃÃes 0, 5, 10, 20 e 25 % do polissacarÃdeo. As soluÃÃes de colÃgeno-polissacarÃdeo sulfatado foram formatadas em moldes de acrÃlico em condiÃÃes de baixa umidade e temperatura. A caracterizaÃÃo fÃsico-quÃmica dos filmes de colÃgeno-polissacarÃdeo sulfatado foi estudada por espectrometria no infravermelho que mostraram bandas tÃpicas do colÃgeno e bandas tÃpicas do polissacarÃdeo sulfatado; a anÃlise tÃrmica por DSC mostra que a integridade estrutural do colÃgeno foi preservada no processo de extraÃÃo e que a adiÃÃo do polissacarÃdeo nas amostras nÃo diminuiu a estabilidade tÃrmica do colÃgeno tendo sido maior na concentraÃÃo de 20 %. Na anÃlise tÃrmica por DTG, a adiÃÃo do polissacarÃdeo causa um pequeno decrÃscimo da estabilidade de degradaÃÃo tÃrmica e ao adicionarmos 5 % do polissacarÃdeo sulfatado ao colÃgeno, ocorre uma reduÃÃo em sua estabilidade, porÃm, a partir daÃ, nas concentraÃÃes 10, 20 e 25 %, a estabilidade de degradaÃÃo tÃrmica do filme vai aumentando. Na espectroscopia de impedÃncia, os resultados estÃo associados, possivelmente, a interaÃÃes entre as macromolÃculas. A compatibilidade sangÃÃnea foi analisada por adesÃo de plaquetas e os resultados mostraram que os filmes possuem hemocompatibilidade e propriedades antitrombogÃnicas. Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgicoFundaÃÃo de Amparo à Pesquisa do Estado do CearÃhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7709application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:20:44Zmail@mail.com -
dc.title.en.fl_str_mv Collagen - sulfated polysaccharide films with antithrombogenic properties: preparation and characterization
dc.title.alternative.pt.fl_str_mv Filmes de colÃgeno - polissacarÃdeo sulfatado como matrizes com propriedades antitrombogÃnicas: preparaÃÃo e caracterizaÃÃo
title Collagen - sulfated polysaccharide films with antithrombogenic properties: preparation and characterization
spellingShingle Collagen - sulfated polysaccharide films with antithrombogenic properties: preparation and characterization
Ana AngÃlica Mathias Macedo
ColÃgeno
PolissacarÃdeo sulfatado
PrÃteses vasculares
Matrizes heparinizadas
collagen
sulfated polysaccharide
cardiovascular prostheses
QUIMICA DOS PRODUTOS NATURAIS
title_short Collagen - sulfated polysaccharide films with antithrombogenic properties: preparation and characterization
title_full Collagen - sulfated polysaccharide films with antithrombogenic properties: preparation and characterization
title_fullStr Collagen - sulfated polysaccharide films with antithrombogenic properties: preparation and characterization
title_full_unstemmed Collagen - sulfated polysaccharide films with antithrombogenic properties: preparation and characterization
title_sort Collagen - sulfated polysaccharide films with antithrombogenic properties: preparation and characterization
author Ana AngÃlica Mathias Macedo
author_facet Ana AngÃlica Mathias Macedo
author_role author
dc.contributor.advisor1.fl_str_mv Renato de Azevedo Moreira
dc.contributor.advisor1ID.fl_str_mv 00114715300
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4451208231291916
dc.contributor.advisor-co1.fl_str_mv SÃnia Duarte FigueirÃ
dc.contributor.advisor-co1ID.fl_str_mv 11258470349
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/2412379131379664
dc.contributor.referee1.fl_str_mv Andrà Luis Coelho da Silva
dc.contributor.referee1ID.fl_str_mv 625 499 953 68
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/3701975151596050
dc.contributor.authorID.fl_str_mv 35853280325
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7219178586824787
dc.contributor.author.fl_str_mv Ana AngÃlica Mathias Macedo
contributor_str_mv Renato de Azevedo Moreira
SÃnia Duarte FigueirÃ
Andrà Luis Coelho da Silva
dc.subject.por.fl_str_mv ColÃgeno
PolissacarÃdeo sulfatado
PrÃteses vasculares
Matrizes heparinizadas
topic ColÃgeno
PolissacarÃdeo sulfatado
PrÃteses vasculares
Matrizes heparinizadas
collagen
sulfated polysaccharide
cardiovascular prostheses
QUIMICA DOS PRODUTOS NATURAIS
dc.subject.eng.fl_str_mv collagen
sulfated polysaccharide
cardiovascular prostheses
dc.subject.cnpq.fl_str_mv QUIMICA DOS PRODUTOS NATURAIS
dc.description.sponsorship.fl_txt_mv Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico
FundaÃÃo de Amparo à Pesquisa do Estado do CearÃ
dc.description.abstract.por.fl_txt_mv This work reports the preparation and characterization of collagen-sulfated polysaccharide films and applications in coating of cardiovascular prostheses. The films were prepared by adding the sulfated polysaccharide solution on soluble collagen (0, 5, 10, 20 and 25 %). The blends of collagen-sulfated polysaccharide were casted in acrylic molds, and dried at low temperature. The physico-chemical properties of collagen-sulfated polysaccharide films, were studied using infrared spectroscopy that showed absorptions typical of collagen and sulphated polysaccharide; the thermal analysis (DSC) showed that the structural integrity of collagen was unmodified during the extraction process and the polysaccharide present in the samples did not decrease the thermal stability of the collagen, which was higher for the concentrations of 20 %. In the thermal analysis (DTG), the addition of the polysaccharide caused a small decrease of the stability of thermal degradation and when was adding 5 % of the sulfated polysaccharide to the collagen, a reduction in its stability occurs, however, in the concentrations of 10, 20 and 25 %, the stability of thermal degradation of the film increased. In the impedance spectroscopy, the results are associated possibly, to the interactions between the macromolecules. The blood compatibility was analyzed by platelet adhesion and the results showed that the films possess hemocompatibility and antitrombogenic properties.
Este trabalho aborda sobre o preparo e a caracterizaÃÃo de filmes de colÃgeno-polissacarÃdeo sulfatado e sua aplicaÃÃo para revestimento de prÃteses vasculares. Os filmes foram obtidos a partir da mistura de soluÃÃo de colÃgeno e polissacarÃdeo sulfatado, nas concentraÃÃes 0, 5, 10, 20 e 25 % do polissacarÃdeo. As soluÃÃes de colÃgeno-polissacarÃdeo sulfatado foram formatadas em moldes de acrÃlico em condiÃÃes de baixa umidade e temperatura. A caracterizaÃÃo fÃsico-quÃmica dos filmes de colÃgeno-polissacarÃdeo sulfatado foi estudada por espectrometria no infravermelho que mostraram bandas tÃpicas do colÃgeno e bandas tÃpicas do polissacarÃdeo sulfatado; a anÃlise tÃrmica por DSC mostra que a integridade estrutural do colÃgeno foi preservada no processo de extraÃÃo e que a adiÃÃo do polissacarÃdeo nas amostras nÃo diminuiu a estabilidade tÃrmica do colÃgeno tendo sido maior na concentraÃÃo de 20 %. Na anÃlise tÃrmica por DTG, a adiÃÃo do polissacarÃdeo causa um pequeno decrÃscimo da estabilidade de degradaÃÃo tÃrmica e ao adicionarmos 5 % do polissacarÃdeo sulfatado ao colÃgeno, ocorre uma reduÃÃo em sua estabilidade, porÃm, a partir daÃ, nas concentraÃÃes 10, 20 e 25 %, a estabilidade de degradaÃÃo tÃrmica do filme vai aumentando. Na espectroscopia de impedÃncia, os resultados estÃo associados, possivelmente, a interaÃÃes entre as macromolÃculas. A compatibilidade sangÃÃnea foi analisada por adesÃo de plaquetas e os resultados mostraram que os filmes possuem hemocompatibilidade e propriedades antitrombogÃnicas.
description This work reports the preparation and characterization of collagen-sulfated polysaccharide films and applications in coating of cardiovascular prostheses. The films were prepared by adding the sulfated polysaccharide solution on soluble collagen (0, 5, 10, 20 and 25 %). The blends of collagen-sulfated polysaccharide were casted in acrylic molds, and dried at low temperature. The physico-chemical properties of collagen-sulfated polysaccharide films, were studied using infrared spectroscopy that showed absorptions typical of collagen and sulphated polysaccharide; the thermal analysis (DSC) showed that the structural integrity of collagen was unmodified during the extraction process and the polysaccharide present in the samples did not decrease the thermal stability of the collagen, which was higher for the concentrations of 20 %. In the thermal analysis (DTG), the addition of the polysaccharide caused a small decrease of the stability of thermal degradation and when was adding 5 % of the sulfated polysaccharide to the collagen, a reduction in its stability occurs, however, in the concentrations of 10, 20 and 25 %, the stability of thermal degradation of the film increased. In the impedance spectroscopy, the results are associated possibly, to the interactions between the macromolecules. The blood compatibility was analyzed by platelet adhesion and the results showed that the films possess hemocompatibility and antitrombogenic properties.
publishDate 2006
dc.date.issued.fl_str_mv 2006-03-03
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
status_str publishedVersion
format masterThesis
dc.identifier.uri.fl_str_mv http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7709
url http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7709
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.publisher.program.fl_str_mv Programa de PÃs-GraduaÃÃo em BioquÃmica
dc.publisher.initials.fl_str_mv UFC
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFC
instname:Universidade Federal do Ceará
instacron:UFC
reponame_str Biblioteca Digital de Teses e Dissertações da UFC
collection Biblioteca Digital de Teses e Dissertações da UFC
instname_str Universidade Federal do Ceará
instacron_str UFC
institution UFC
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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