Preconditioning nutraceutical with mixtures of omega oils 3, 6 and 9 on acute zymosan-induced arthritis in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=12253 |
Resumo: | Artritis is an inflammatory condition that affects synovial articulations. The most relevant manifestations are decreased pain threshold and edema. A possible alternative as adjuvant therapy to this disease is the inclusion of nutraceutics in the diet. In the present study preconditioning with mixes of oils omega 3, 6 and 9 was used at an experimental model of acute artritis induced by zymosan (Zy) in rats. The objective of this study was to evaluate the antiinflammatory and antinociceptive effects of these mixes which contain high omega- 9:omega-6 ratio (3.4:1) and low omega-6:omega-3 ratio (1.4:1). These oil mixes contained different sources of omega-3: Mix 1 with alfa-linolenic acid, Mix 2 with alfa-linolenic, eicosapentanoic and docosaexanoic acids, and Mix 3 with alfa-linolenic and docosaexanoic acids. The monoinsaturated omega-9 fatty acids present antioxidant action whereas the poliinsaturated fatty acids omega-3 present antiinflammatory effects and the omega-6 fatty acids are proinflammatory. Thirty male wistar rats weighing 180-200g were used, for each experimental group. The animals were randomly distributed in two groups: Control (=12) and Test (n=18). The Control group was subdivided into groups: Negative Control whose animals received water orogastrically during 7 days (n=6), and Positive Controls whose animals received no oil mixes during 7 days but were treated with either dexametazone (DEXA â n=6) at the experiment day (day 8). The Test group was distributed into three groups of 6 rats subjected to orgastric administration of mixes 1, 2 and 3. On day 8 all animals had artritis induction by intraarticular injection of zymosan (1mg/50microlitters) in the right knee. Analised variables were articular incapacitation (A.I.), leucocyte migration, mieloperoxidase (MPO) activity, articular edema, vascular permeability, articular capsule immunostain for iNOS and NF-kB and histopathological analysis. Hypernociception was induced by carrageenan and PGE2. Results demonstrated a significant decrease (p<0.05) in A.I. during the third hour in animals preconditioned with mixes 1, 2 and 3, to level similar to that of those treated with DEXA. As concerned to leucocyte migration, there was a significant reduction (p<0.05) in the number of leucocytes in the aticular wash in rats preconditioned with all three mixes, as well as decreased activity of MPO (p<0.05) similar to what was found in animals treated with DEXA. Edema was inhibited and vascular permeability diminished in all groups preconditioned with all mixes (p<0.05). In animals of Mix 3 the decrease in vascular permeability was even more pronounced as compared to all other groups (p<0.001). Histopathology analysis showed a decrease in cellular inflitration and prevention from loss of articular capsule integrity in rats preconditioned with mixes 1 and 2. Immunohistochemical stain for iNOS and NFkB was significantly smaller in rats of groups 1, 2 and 3, similar to that of DEXA group. All oil mixes showed a significant inhibitory effect (p<0.05) as concerned to antinociception through the use of a model of plantar mecanic hiperalgia induced by carrageenan, similar to that of rats of INDO group, a drug used in the positive control . On the other hand, the same did not occur when hiperalgia was induced by PGE2. The results therefore suggest that preconditioning with mixes 1, 2 and 3 present antiinflammatory and antinociceptive effects, as well as decreases synovial expression of iNOS and NFkB. However, the observed antinociceptive effect appears to be indirect and due to the antiinflammatory effect. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPreconditioning nutraceutical with mixtures of omega oils 3, 6 and 9 on acute zymosan-induced arthritis in ratsPrÃ-condicionamento nutracÃutico com misturas de Ãleos Ãmega 3, 6 e 9 na artrite aguda induzida por zymosan em ratos2014-04-11Paulo Roberto LeitÃo de Vasconcelos11852844353http://buscatextual.cnpq.br/buscatextual/servletrecuperafoto?id=K4787736J6Mariana Lima Vale69461538391http://lattes.cnpq.br/2233181081815735SÃrgio Botelho GuimarÃes02855089387http://lattes.cnpq.br/6819610160280901Maria Luisa Pereira de Melo25968998334http://lattes.cnpq.br/557617491486892600022177329http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4290182H2Beatriz Torres de Melo Cavalcante Universidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em CirurgiaUFCBRCIRURGIAArtritis is an inflammatory condition that affects synovial articulations. The most relevant manifestations are decreased pain threshold and edema. A possible alternative as adjuvant therapy to this disease is the inclusion of nutraceutics in the diet. In the present study preconditioning with mixes of oils omega 3, 6 and 9 was used at an experimental model of acute artritis induced by zymosan (Zy) in rats. The objective of this study was to evaluate the antiinflammatory and antinociceptive effects of these mixes which contain high omega- 9:omega-6 ratio (3.4:1) and low omega-6:omega-3 ratio (1.4:1). These oil mixes contained different sources of omega-3: Mix 1 with alfa-linolenic acid, Mix 2 with alfa-linolenic, eicosapentanoic and docosaexanoic acids, and Mix 3 with alfa-linolenic and docosaexanoic acids. The monoinsaturated omega-9 fatty acids present antioxidant action whereas the poliinsaturated fatty acids omega-3 present antiinflammatory effects and the omega-6 fatty acids are proinflammatory. Thirty male wistar rats weighing 180-200g were used, for each experimental group. The animals were randomly distributed in two groups: Control (=12) and Test (n=18). The Control group was subdivided into groups: Negative Control whose animals received water orogastrically during 7 days (n=6), and Positive Controls whose animals received no oil mixes during 7 days but were treated with either dexametazone (DEXA â n=6) at the experiment day (day 8). The Test group was distributed into three groups of 6 rats subjected to orgastric administration of mixes 1, 2 and 3. On day 8 all animals had artritis induction by intraarticular injection of zymosan (1mg/50microlitters) in the right knee. Analised variables were articular incapacitation (A.I.), leucocyte migration, mieloperoxidase (MPO) activity, articular edema, vascular permeability, articular capsule immunostain for iNOS and NF-kB and histopathological analysis. Hypernociception was induced by carrageenan and PGE2. Results demonstrated a significant decrease (p<0.05) in A.I. during the third hour in animals preconditioned with mixes 1, 2 and 3, to level similar to that of those treated with DEXA. As concerned to leucocyte migration, there was a significant reduction (p<0.05) in the number of leucocytes in the aticular wash in rats preconditioned with all three mixes, as well as decreased activity of MPO (p<0.05) similar to what was found in animals treated with DEXA. Edema was inhibited and vascular permeability diminished in all groups preconditioned with all mixes (p<0.05). In animals of Mix 3 the decrease in vascular permeability was even more pronounced as compared to all other groups (p<0.001). Histopathology analysis showed a decrease in cellular inflitration and prevention from loss of articular capsule integrity in rats preconditioned with mixes 1 and 2. Immunohistochemical stain for iNOS and NFkB was significantly smaller in rats of groups 1, 2 and 3, similar to that of DEXA group. All oil mixes showed a significant inhibitory effect (p<0.05) as concerned to antinociception through the use of a model of plantar mecanic hiperalgia induced by carrageenan, similar to that of rats of INDO group, a drug used in the positive control . On the other hand, the same did not occur when hiperalgia was induced by PGE2. The results therefore suggest that preconditioning with mixes 1, 2 and 3 present antiinflammatory and antinociceptive effects, as well as decreases synovial expression of iNOS and NFkB. However, the observed antinociceptive effect appears to be indirect and due to the antiinflammatory effect.A artrite à uma condiÃÃo inflamatÃria que afeta as articulaÃÃes sinoviais. Os sintomas mais relevantes sÃo aumento da sensibilidade à dor nas articulaÃÃes e edema. Uma possÃvel alternativa para o tratamento adjuvante da doenÃa à a inclusÃo de nutracÃuticos na dieta oral. No presente estudo foi realizado o prÃ-condicionamento com misturas de Ãleos Ãmega 3, 6 e 9 em um modelo experimental de artrite aguda em ratos por zymosan (Zy). O objetivo deste trabalho foi avaliar os efeitos anti-inflamatÃrio e antinociceptivo das misturas (MIX) de Ãleos, contendo elevada relaÃÃo -9/-6 (3,4:1) e baixa relaÃÃo -6/-3 (1,4:1) na artrite por Zy. As misturas oleosas continham diferentes fontes de -3: MIX-1, contendo o Ãcido -linolÃnico; MIX-2, os Ãcidos -linolÃnico, eicosapentaenÃico e docosaexaenÃico, e o MIX-3, os Ãcidos - linolÃnico e docosaexaenÃico. Os Ãcidos graxos monoinsaturados -9 tÃm aÃÃo antioxidante, os poliinsaturados -3 possuem aÃÃo anti-inflamatÃria, enquanto os -6 sÃo prÃinflamatÃrios. Foram utilizados 30 ratos Wistar machos com peso mÃdio corporal de 180-200 gramas, para cada grupo experimental. Os animais foram distribuÃdos, aleatoriamente, em dois grupos: o Controle (n=12) e o Teste (n=18). O grupo controle foi subdividido em dois grupos: Controle negativo cujos animais receberam Ãgua, por via orogÃstrica, durante 7 dias (n=6). Controle positivo, cujos animais nÃo receberam nenhum dos MIX, durante esses sete dias e foram tratados com dexametasona (DEXA- n=6) no dia do experimento (dia 8). O grupo Teste foi subdividido em trÃs grupos de 6 animais submetidos à administraÃÃo orogÃstrica de MIX-1, MIX-2 e MIX-3, respectivamente. No 8o dia, em todos os grupos (Controle e Teste) foi induzida a artrite por meio de uma injeÃÃo intrarticular (i.a.) de zymosan (1mg/50μL) no joelho direito. As variÃveis avaliadas foram: a incapacitaÃÃo articular (I.A.), migraÃÃo de leucÃcitos, atividade de mieloperoxidase (MPO), edema articular, permeabilidade vascular, imunomarcaÃÃo de iNOS e NF-kB e anÃlise histopatolÃgica. Ainda foram realizados os testes de hipernocicepÃÃo induzida por carragenina e PGE2. Os resultados demonstraram uma diminuiÃÃo significante (p<0,05), durante a 3 hora da I.A. (pico de incapacitaÃÃo) de todos os grupos prÃ-condicionados com as misturas (MIX-1, 2 e 3) à semelhanÃa do controle positivo por DEXA. Em relaÃÃo à migraÃÃo de leucÃcitos para o lÃquido sinovial, todos os grupos prÃ-condicionados com (MIX-1, 2, e 3) apresentaram significante reduÃÃo da migraÃÃo (p<0,05) acompanhado da diminuiÃÃo do nÃmero de neutrÃfilos e da atividade da MPO (p<0,05) à semelhanÃa do grupo tratado com DEXA. O edema foi inibido (p<0,05) assim como, tambÃm, houve uma reduÃÃo da permeabilidade vascular em todos os grupos prÃ-condicionados com diferenÃas significantes (p<0,05), sendo o efeito maior do MIX-3 (p<0,001) em relaÃÃo aos outros. A anÃlise histopatolÃgica demonstrou uma diminuiÃÃo do infiltrado celular e prevenÃÃo da perda da integridade da cÃpsula articular de forma significante (p<0,05) para os MIX-1 e 2. A marcaÃÃo para iNOS e NF-kB mostrou uma diminuiÃÃo nos grupos MIX-1, 2 e 3, igualmente como no grupo da DEXA. Na anÃlise do efeito antinociceptivo no modelo de hiperalgesia mecÃnica plantar induzido por carragenina, todos os MIX demonstraram efeito inibitÃrio significante (p<0,05), assim como a indometacina, fÃrmaco usado no controle positivo. Entretanto, isso nÃo foi observado na hiperalgesia induzida por PGE2. Os resultados sugerem que o prÃcondicionamento com os MIX- 1, 2 e 3 possuem efeitos antinociceptivos e anti-inflamatÃrios, diminuindo, tambÃm, a expressÃo de iNOS e NF-kB no tecido sinovial. PorÃm, o efeito antinociceptivo parece ser indireto e decorrente do seu efeito anti-inflamatÃrio.http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=12253application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:25:26Zmail@mail.com - |
dc.title.en.fl_str_mv |
Preconditioning nutraceutical with mixtures of omega oils 3, 6 and 9 on acute zymosan-induced arthritis in rats |
dc.title.alternative.pt.fl_str_mv |
PrÃ-condicionamento nutracÃutico com misturas de Ãleos Ãmega 3, 6 e 9 na artrite aguda induzida por zymosan em ratos |
title |
Preconditioning nutraceutical with mixtures of omega oils 3, 6 and 9 on acute zymosan-induced arthritis in rats |
spellingShingle |
Preconditioning nutraceutical with mixtures of omega oils 3, 6 and 9 on acute zymosan-induced arthritis in rats Beatriz Torres de Melo Cavalcante CIRURGIA |
title_short |
Preconditioning nutraceutical with mixtures of omega oils 3, 6 and 9 on acute zymosan-induced arthritis in rats |
title_full |
Preconditioning nutraceutical with mixtures of omega oils 3, 6 and 9 on acute zymosan-induced arthritis in rats |
title_fullStr |
Preconditioning nutraceutical with mixtures of omega oils 3, 6 and 9 on acute zymosan-induced arthritis in rats |
title_full_unstemmed |
Preconditioning nutraceutical with mixtures of omega oils 3, 6 and 9 on acute zymosan-induced arthritis in rats |
title_sort |
Preconditioning nutraceutical with mixtures of omega oils 3, 6 and 9 on acute zymosan-induced arthritis in rats |
author |
Beatriz Torres de Melo Cavalcante |
author_facet |
Beatriz Torres de Melo Cavalcante |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Paulo Roberto LeitÃo de Vasconcelos |
dc.contributor.advisor1ID.fl_str_mv |
11852844353 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/servletrecuperafoto?id=K4787736J6 |
dc.contributor.advisor-co1.fl_str_mv |
Mariana Lima Vale |
dc.contributor.advisor-co1ID.fl_str_mv |
69461538391 |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2233181081815735 |
dc.contributor.referee1.fl_str_mv |
SÃrgio Botelho GuimarÃes |
dc.contributor.referee1ID.fl_str_mv |
02855089387 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6819610160280901 |
dc.contributor.referee2.fl_str_mv |
Maria Luisa Pereira de Melo |
dc.contributor.referee2ID.fl_str_mv |
25968998334 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5576174914868926 |
dc.contributor.authorID.fl_str_mv |
00022177329 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4290182H2 |
dc.contributor.author.fl_str_mv |
Beatriz Torres de Melo Cavalcante |
contributor_str_mv |
Paulo Roberto LeitÃo de Vasconcelos Mariana Lima Vale SÃrgio Botelho GuimarÃes Maria Luisa Pereira de Melo |
dc.subject.cnpq.fl_str_mv |
CIRURGIA |
topic |
CIRURGIA |
dc.description.abstract.por.fl_txt_mv |
Artritis is an inflammatory condition that affects synovial articulations. The most relevant manifestations are decreased pain threshold and edema. A possible alternative as adjuvant therapy to this disease is the inclusion of nutraceutics in the diet. In the present study preconditioning with mixes of oils omega 3, 6 and 9 was used at an experimental model of acute artritis induced by zymosan (Zy) in rats. The objective of this study was to evaluate the antiinflammatory and antinociceptive effects of these mixes which contain high omega- 9:omega-6 ratio (3.4:1) and low omega-6:omega-3 ratio (1.4:1). These oil mixes contained different sources of omega-3: Mix 1 with alfa-linolenic acid, Mix 2 with alfa-linolenic, eicosapentanoic and docosaexanoic acids, and Mix 3 with alfa-linolenic and docosaexanoic acids. The monoinsaturated omega-9 fatty acids present antioxidant action whereas the poliinsaturated fatty acids omega-3 present antiinflammatory effects and the omega-6 fatty acids are proinflammatory. Thirty male wistar rats weighing 180-200g were used, for each experimental group. The animals were randomly distributed in two groups: Control (=12) and Test (n=18). The Control group was subdivided into groups: Negative Control whose animals received water orogastrically during 7 days (n=6), and Positive Controls whose animals received no oil mixes during 7 days but were treated with either dexametazone (DEXA â n=6) at the experiment day (day 8). The Test group was distributed into three groups of 6 rats subjected to orgastric administration of mixes 1, 2 and 3. On day 8 all animals had artritis induction by intraarticular injection of zymosan (1mg/50microlitters) in the right knee. Analised variables were articular incapacitation (A.I.), leucocyte migration, mieloperoxidase (MPO) activity, articular edema, vascular permeability, articular capsule immunostain for iNOS and NF-kB and histopathological analysis. Hypernociception was induced by carrageenan and PGE2. Results demonstrated a significant decrease (p<0.05) in A.I. during the third hour in animals preconditioned with mixes 1, 2 and 3, to level similar to that of those treated with DEXA. As concerned to leucocyte migration, there was a significant reduction (p<0.05) in the number of leucocytes in the aticular wash in rats preconditioned with all three mixes, as well as decreased activity of MPO (p<0.05) similar to what was found in animals treated with DEXA. Edema was inhibited and vascular permeability diminished in all groups preconditioned with all mixes (p<0.05). In animals of Mix 3 the decrease in vascular permeability was even more pronounced as compared to all other groups (p<0.001). Histopathology analysis showed a decrease in cellular inflitration and prevention from loss of articular capsule integrity in rats preconditioned with mixes 1 and 2. Immunohistochemical stain for iNOS and NFkB was significantly smaller in rats of groups 1, 2 and 3, similar to that of DEXA group. All oil mixes showed a significant inhibitory effect (p<0.05) as concerned to antinociception through the use of a model of plantar mecanic hiperalgia induced by carrageenan, similar to that of rats of INDO group, a drug used in the positive control . On the other hand, the same did not occur when hiperalgia was induced by PGE2. The results therefore suggest that preconditioning with mixes 1, 2 and 3 present antiinflammatory and antinociceptive effects, as well as decreases synovial expression of iNOS and NFkB. However, the observed antinociceptive effect appears to be indirect and due to the antiinflammatory effect. A artrite à uma condiÃÃo inflamatÃria que afeta as articulaÃÃes sinoviais. Os sintomas mais relevantes sÃo aumento da sensibilidade à dor nas articulaÃÃes e edema. Uma possÃvel alternativa para o tratamento adjuvante da doenÃa à a inclusÃo de nutracÃuticos na dieta oral. No presente estudo foi realizado o prÃ-condicionamento com misturas de Ãleos Ãmega 3, 6 e 9 em um modelo experimental de artrite aguda em ratos por zymosan (Zy). O objetivo deste trabalho foi avaliar os efeitos anti-inflamatÃrio e antinociceptivo das misturas (MIX) de Ãleos, contendo elevada relaÃÃo -9/-6 (3,4:1) e baixa relaÃÃo -6/-3 (1,4:1) na artrite por Zy. As misturas oleosas continham diferentes fontes de -3: MIX-1, contendo o Ãcido -linolÃnico; MIX-2, os Ãcidos -linolÃnico, eicosapentaenÃico e docosaexaenÃico, e o MIX-3, os Ãcidos - linolÃnico e docosaexaenÃico. Os Ãcidos graxos monoinsaturados -9 tÃm aÃÃo antioxidante, os poliinsaturados -3 possuem aÃÃo anti-inflamatÃria, enquanto os -6 sÃo prÃinflamatÃrios. Foram utilizados 30 ratos Wistar machos com peso mÃdio corporal de 180-200 gramas, para cada grupo experimental. Os animais foram distribuÃdos, aleatoriamente, em dois grupos: o Controle (n=12) e o Teste (n=18). O grupo controle foi subdividido em dois grupos: Controle negativo cujos animais receberam Ãgua, por via orogÃstrica, durante 7 dias (n=6). Controle positivo, cujos animais nÃo receberam nenhum dos MIX, durante esses sete dias e foram tratados com dexametasona (DEXA- n=6) no dia do experimento (dia 8). O grupo Teste foi subdividido em trÃs grupos de 6 animais submetidos à administraÃÃo orogÃstrica de MIX-1, MIX-2 e MIX-3, respectivamente. No 8o dia, em todos os grupos (Controle e Teste) foi induzida a artrite por meio de uma injeÃÃo intrarticular (i.a.) de zymosan (1mg/50μL) no joelho direito. As variÃveis avaliadas foram: a incapacitaÃÃo articular (I.A.), migraÃÃo de leucÃcitos, atividade de mieloperoxidase (MPO), edema articular, permeabilidade vascular, imunomarcaÃÃo de iNOS e NF-kB e anÃlise histopatolÃgica. Ainda foram realizados os testes de hipernocicepÃÃo induzida por carragenina e PGE2. Os resultados demonstraram uma diminuiÃÃo significante (p<0,05), durante a 3 hora da I.A. (pico de incapacitaÃÃo) de todos os grupos prÃ-condicionados com as misturas (MIX-1, 2 e 3) à semelhanÃa do controle positivo por DEXA. Em relaÃÃo à migraÃÃo de leucÃcitos para o lÃquido sinovial, todos os grupos prÃ-condicionados com (MIX-1, 2, e 3) apresentaram significante reduÃÃo da migraÃÃo (p<0,05) acompanhado da diminuiÃÃo do nÃmero de neutrÃfilos e da atividade da MPO (p<0,05) à semelhanÃa do grupo tratado com DEXA. O edema foi inibido (p<0,05) assim como, tambÃm, houve uma reduÃÃo da permeabilidade vascular em todos os grupos prÃ-condicionados com diferenÃas significantes (p<0,05), sendo o efeito maior do MIX-3 (p<0,001) em relaÃÃo aos outros. A anÃlise histopatolÃgica demonstrou uma diminuiÃÃo do infiltrado celular e prevenÃÃo da perda da integridade da cÃpsula articular de forma significante (p<0,05) para os MIX-1 e 2. A marcaÃÃo para iNOS e NF-kB mostrou uma diminuiÃÃo nos grupos MIX-1, 2 e 3, igualmente como no grupo da DEXA. Na anÃlise do efeito antinociceptivo no modelo de hiperalgesia mecÃnica plantar induzido por carragenina, todos os MIX demonstraram efeito inibitÃrio significante (p<0,05), assim como a indometacina, fÃrmaco usado no controle positivo. Entretanto, isso nÃo foi observado na hiperalgesia induzida por PGE2. Os resultados sugerem que o prÃcondicionamento com os MIX- 1, 2 e 3 possuem efeitos antinociceptivos e anti-inflamatÃrios, diminuindo, tambÃm, a expressÃo de iNOS e NF-kB no tecido sinovial. PorÃm, o efeito antinociceptivo parece ser indireto e decorrente do seu efeito anti-inflamatÃrio. |
description |
Artritis is an inflammatory condition that affects synovial articulations. The most relevant manifestations are decreased pain threshold and edema. A possible alternative as adjuvant therapy to this disease is the inclusion of nutraceutics in the diet. In the present study preconditioning with mixes of oils omega 3, 6 and 9 was used at an experimental model of acute artritis induced by zymosan (Zy) in rats. The objective of this study was to evaluate the antiinflammatory and antinociceptive effects of these mixes which contain high omega- 9:omega-6 ratio (3.4:1) and low omega-6:omega-3 ratio (1.4:1). These oil mixes contained different sources of omega-3: Mix 1 with alfa-linolenic acid, Mix 2 with alfa-linolenic, eicosapentanoic and docosaexanoic acids, and Mix 3 with alfa-linolenic and docosaexanoic acids. The monoinsaturated omega-9 fatty acids present antioxidant action whereas the poliinsaturated fatty acids omega-3 present antiinflammatory effects and the omega-6 fatty acids are proinflammatory. Thirty male wistar rats weighing 180-200g were used, for each experimental group. The animals were randomly distributed in two groups: Control (=12) and Test (n=18). The Control group was subdivided into groups: Negative Control whose animals received water orogastrically during 7 days (n=6), and Positive Controls whose animals received no oil mixes during 7 days but were treated with either dexametazone (DEXA â n=6) at the experiment day (day 8). The Test group was distributed into three groups of 6 rats subjected to orgastric administration of mixes 1, 2 and 3. On day 8 all animals had artritis induction by intraarticular injection of zymosan (1mg/50microlitters) in the right knee. Analised variables were articular incapacitation (A.I.), leucocyte migration, mieloperoxidase (MPO) activity, articular edema, vascular permeability, articular capsule immunostain for iNOS and NF-kB and histopathological analysis. Hypernociception was induced by carrageenan and PGE2. Results demonstrated a significant decrease (p<0.05) in A.I. during the third hour in animals preconditioned with mixes 1, 2 and 3, to level similar to that of those treated with DEXA. As concerned to leucocyte migration, there was a significant reduction (p<0.05) in the number of leucocytes in the aticular wash in rats preconditioned with all three mixes, as well as decreased activity of MPO (p<0.05) similar to what was found in animals treated with DEXA. Edema was inhibited and vascular permeability diminished in all groups preconditioned with all mixes (p<0.05). In animals of Mix 3 the decrease in vascular permeability was even more pronounced as compared to all other groups (p<0.001). Histopathology analysis showed a decrease in cellular inflitration and prevention from loss of articular capsule integrity in rats preconditioned with mixes 1 and 2. Immunohistochemical stain for iNOS and NFkB was significantly smaller in rats of groups 1, 2 and 3, similar to that of DEXA group. All oil mixes showed a significant inhibitory effect (p<0.05) as concerned to antinociception through the use of a model of plantar mecanic hiperalgia induced by carrageenan, similar to that of rats of INDO group, a drug used in the positive control . On the other hand, the same did not occur when hiperalgia was induced by PGE2. The results therefore suggest that preconditioning with mixes 1, 2 and 3 present antiinflammatory and antinociceptive effects, as well as decreases synovial expression of iNOS and NFkB. However, the observed antinociceptive effect appears to be indirect and due to the antiinflammatory effect. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-04-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
status_str |
publishedVersion |
format |
masterThesis |
dc.identifier.uri.fl_str_mv |
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=12253 |
url |
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=12253 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Cearà |
dc.publisher.program.fl_str_mv |
Programa de PÃs-GraduaÃÃo em Cirurgia |
dc.publisher.initials.fl_str_mv |
UFC |
dc.publisher.country.fl_str_mv |
BR |
publisher.none.fl_str_mv |
Universidade Federal do Cearà |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFC instname:Universidade Federal do Ceará instacron:UFC |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFC |
collection |
Biblioteca Digital de Teses e Dissertações da UFC |
instname_str |
Universidade Federal do Ceará |
instacron_str |
UFC |
institution |
UFC |
repository.name.fl_str_mv |
-
|
repository.mail.fl_str_mv |
mail@mail.com |
_version_ |
1643295191367417856 |