Biotechnological potential of lectins: induction of apoptosis in A549 cells, pro-healing effects on experimental wounds and cell toxicity analysis on Artemia sp.

Detalhes bibliográficos
Autor(a) principal: Francisco Vassiliepe Sousa Arruda
Data de Publicação: 2011
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11065
Resumo: This work evaluated the biotechnological potential of lectins in the induction of apoptosis of neoplastic cells; the effect on wound healing in a murine model (in vivo) and established the level of toxicity on Artemia sp. Lectins are proteins or glycoproteins ubiquitously distributed in nature, being found from bacteria to humans. Since carbohydrates can act as mediators of biological information, the interaction with lectins can trigger some biologically important effect. To verify the role of lectins in the induction of apoptosis, A549 cells (lung carcinoma) were grown for 1 hour with lectins isolated from seeds of Canavalia ensiformis (ConA), C. brasiliensis (ConBr), C. boliviana (ConBol), C. grandiflora (Cgran) and C. gladiata (CGL) labeled with FITC. In addition, the relative gene expression of p53, Bax, Bcl-2 and caspase 9 was also evaluated when the cells were cultured with ConA, ConBr and ConBol. The data showed that all lectins interacted with A549 cells. Nevertheless, p53 and Bax had no significant change. Unlike, the expression of BCL-2 was down-regulated while caspase 9 was up-regulated by treatment with lectins. Concerning the pro-healing potential, the lectin isolated from the red alga Bryothamnion seafortii (BS) was tested by topical treatment of skin lesions. The data showed that BS improved the quality of healing, probably through the induction of fibroblast proliferation. Finally, the lethality test of Artemia sp. was carried out to determine the toxicity of some Diocleinae lectins. ConBr is the most toxic lectin against Artemia, while ConA is the least toxic, and ConBol, ConM, ConGr and CGL exhibit intermediate levels of toxicity.
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spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisBiotechnological potential of lectins: induction of apoptosis in A549 cells, pro-healing effects on experimental wounds and cell toxicity analysis on Artemia sp.Potencial biotecnolÃgico de lectinas: induÃÃo de apoptose em cÃlulas A549, efeito prÃ-cicatrizante em feridas experimentais e anÃlise de toxicidade sobre Artemia sp.2011-11-29Edson Holanda Teixeira71755985304http://lattes.cnpq.br/8800103074322967 Benildo Sousa Cavada24242349068http://lattes.cnpq.br/5029704662813380 Kyria Santiago do Nascimento08671084795http://lattes.cnpq.br/4999713109630711Jorge Luiz Martins24255807000http://lattes.cnpq.br/4207988938663438 Helcio Silva dos Santos77045050330http://lattes.cnpq.br/347884205498449789025075304http://lattes.cnpq.br/1724172190095569Francisco Vassiliepe Sousa ArrudaUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em Biotecnologia (Rede Nordeste de Biotecnologia - RENORBIO)UFCBRLectinas Diocleinae Apoptose CicatrizaÃÃo Toxicidade Artemia A549Lectin Diocleinae Apoptosis Wound healing Toxicity Artemia A549IMUNOLOGIAThis work evaluated the biotechnological potential of lectins in the induction of apoptosis of neoplastic cells; the effect on wound healing in a murine model (in vivo) and established the level of toxicity on Artemia sp. Lectins are proteins or glycoproteins ubiquitously distributed in nature, being found from bacteria to humans. Since carbohydrates can act as mediators of biological information, the interaction with lectins can trigger some biologically important effect. To verify the role of lectins in the induction of apoptosis, A549 cells (lung carcinoma) were grown for 1 hour with lectins isolated from seeds of Canavalia ensiformis (ConA), C. brasiliensis (ConBr), C. boliviana (ConBol), C. grandiflora (Cgran) and C. gladiata (CGL) labeled with FITC. In addition, the relative gene expression of p53, Bax, Bcl-2 and caspase 9 was also evaluated when the cells were cultured with ConA, ConBr and ConBol. The data showed that all lectins interacted with A549 cells. Nevertheless, p53 and Bax had no significant change. Unlike, the expression of BCL-2 was down-regulated while caspase 9 was up-regulated by treatment with lectins. Concerning the pro-healing potential, the lectin isolated from the red alga Bryothamnion seafortii (BS) was tested by topical treatment of skin lesions. The data showed that BS improved the quality of healing, probably through the induction of fibroblast proliferation. Finally, the lethality test of Artemia sp. was carried out to determine the toxicity of some Diocleinae lectins. ConBr is the most toxic lectin against Artemia, while ConA is the least toxic, and ConBol, ConM, ConGr and CGL exhibit intermediate levels of toxicity.Este trabalho avaliou o potencial biotecnolÃgico de lectinas na induÃÃo da apoptose de cÃlulas neoplÃsicas, o efeito sobre a cicatrizaÃÃo de feridas em modelo murino (in vivo) e estabeleceu o nÃvel de toxicidade sobre Artemia sp. Lectinas sÃo proteÃnas ou glicoproteÃnas distribuÃdas ubiquamente na natureza, sendo encontradas desde bactÃrias atà seres humanos. Tais proteÃnas possuem a capacidade de ligar-se seletivamente a carboidratos. Considerando que carboidratos possam atuar como mediadores da informaÃÃo biolÃgica, a sua interaÃÃo com lectinas pode desencadear algum efeito biolÃgico importante. Para verificar o papel das lectinas sobre a induÃÃo da apoptose, cÃlulas A549 (carcinoma de pulmÃo) foram cultivadas durante 1 hora juntamente com lectinas isoladas das sementes de Canavalia ensiformis (ConA), C. brasiliensis (ConBr), C. boliviana (ConBol), C. grandiflora (Cgran) e C. gladiata (CGL) marcadas com FITC. Em adiÃÃo, a expressÃo gÃnica relativa de p53, Bax, BCL-2 e caspase 9 foi tambÃm avaliada quando as cÃlulas foram cultivadas com ConA, ConBr e ConBol. Os dados mostraram que todas as lectinas intergiram com as cÃlulas A549. NÃo obstante, p53 e Bax nÃo tiveram nenhuma alteraÃÃo significativa. PorÃm, BCL-2 teve sua expressÃo diminuÃda enquanto a da caspase 9 foi aumentada pelo tratamento com as lectinas. Com relaÃÃo ao potencial pro-cicatrizante, a lectina isolada da alga vermelha Bryothamnion seafortii (BS) foi testada atravÃs do tratamento tÃpico de lesÃes cutÃneas. Os dados mostraram que BS melhora a qualidade da cicatrizaÃÃo, muito possivelmente atravÃs da induÃÃo da proliferaÃÃo de fibroblastos. Por fim, o teste de letalidade sobre Artemia sp. foi utilizado para verificar a toxicidade de algumas lectinas da subtribo Diocleinae. Os resultados mostraran que ConBr e ConA sÃo as lectinas mais tÃxica e menos tÃxica, respectivamente. ConBol, ConM, Cgran e CGL exibiram nÃveis intermediÃrios de toxicidade.FundaÃÃo de Amparo à Pesquisa do Estado do CearÃhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11065application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:24:13Zmail@mail.com -
dc.title.en.fl_str_mv Biotechnological potential of lectins: induction of apoptosis in A549 cells, pro-healing effects on experimental wounds and cell toxicity analysis on Artemia sp.
dc.title.alternative.pt.fl_str_mv Potencial biotecnolÃgico de lectinas: induÃÃo de apoptose em cÃlulas A549, efeito prÃ-cicatrizante em feridas experimentais e anÃlise de toxicidade sobre Artemia sp.
title Biotechnological potential of lectins: induction of apoptosis in A549 cells, pro-healing effects on experimental wounds and cell toxicity analysis on Artemia sp.
spellingShingle Biotechnological potential of lectins: induction of apoptosis in A549 cells, pro-healing effects on experimental wounds and cell toxicity analysis on Artemia sp.
Francisco Vassiliepe Sousa Arruda
Lectinas
Diocleinae
Apoptose
CicatrizaÃÃo
Toxicidade
Artemia
A549
Lectin
Diocleinae
Apoptosis
Wound healing
Toxicity
Artemia
A549
IMUNOLOGIA
title_short Biotechnological potential of lectins: induction of apoptosis in A549 cells, pro-healing effects on experimental wounds and cell toxicity analysis on Artemia sp.
title_full Biotechnological potential of lectins: induction of apoptosis in A549 cells, pro-healing effects on experimental wounds and cell toxicity analysis on Artemia sp.
title_fullStr Biotechnological potential of lectins: induction of apoptosis in A549 cells, pro-healing effects on experimental wounds and cell toxicity analysis on Artemia sp.
title_full_unstemmed Biotechnological potential of lectins: induction of apoptosis in A549 cells, pro-healing effects on experimental wounds and cell toxicity analysis on Artemia sp.
title_sort Biotechnological potential of lectins: induction of apoptosis in A549 cells, pro-healing effects on experimental wounds and cell toxicity analysis on Artemia sp.
author Francisco Vassiliepe Sousa Arruda
author_facet Francisco Vassiliepe Sousa Arruda
author_role author
dc.contributor.advisor1.fl_str_mv Edson Holanda Teixeira
dc.contributor.advisor1ID.fl_str_mv 71755985304
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8800103074322967
dc.contributor.advisor-co1.fl_str_mv Benildo Sousa Cavada
dc.contributor.advisor-co1ID.fl_str_mv 24242349068
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/5029704662813380
dc.contributor.referee1.fl_str_mv Kyria Santiago do Nascimento
dc.contributor.referee1ID.fl_str_mv 08671084795
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/4999713109630711
dc.contributor.referee2.fl_str_mv Jorge Luiz Martins
dc.contributor.referee2ID.fl_str_mv 24255807000
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/4207988938663438
dc.contributor.referee3.fl_str_mv Helcio Silva dos Santos
dc.contributor.referee3ID.fl_str_mv 77045050330
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/3478842054984497
dc.contributor.authorID.fl_str_mv 89025075304
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1724172190095569
dc.contributor.author.fl_str_mv Francisco Vassiliepe Sousa Arruda
contributor_str_mv Edson Holanda Teixeira
Benildo Sousa Cavada
Kyria Santiago do Nascimento
Jorge Luiz Martins
Helcio Silva dos Santos
dc.subject.por.fl_str_mv Lectinas
Diocleinae
Apoptose
CicatrizaÃÃo
Toxicidade
Artemia
A549
topic Lectinas
Diocleinae
Apoptose
CicatrizaÃÃo
Toxicidade
Artemia
A549
Lectin
Diocleinae
Apoptosis
Wound healing
Toxicity
Artemia
A549
IMUNOLOGIA
dc.subject.eng.fl_str_mv Lectin
Diocleinae
Apoptosis
Wound healing
Toxicity
Artemia
A549
dc.subject.cnpq.fl_str_mv IMUNOLOGIA
dc.description.sponsorship.fl_txt_mv FundaÃÃo de Amparo à Pesquisa do Estado do CearÃ
dc.description.abstract.por.fl_txt_mv This work evaluated the biotechnological potential of lectins in the induction of apoptosis of neoplastic cells; the effect on wound healing in a murine model (in vivo) and established the level of toxicity on Artemia sp. Lectins are proteins or glycoproteins ubiquitously distributed in nature, being found from bacteria to humans. Since carbohydrates can act as mediators of biological information, the interaction with lectins can trigger some biologically important effect. To verify the role of lectins in the induction of apoptosis, A549 cells (lung carcinoma) were grown for 1 hour with lectins isolated from seeds of Canavalia ensiformis (ConA), C. brasiliensis (ConBr), C. boliviana (ConBol), C. grandiflora (Cgran) and C. gladiata (CGL) labeled with FITC. In addition, the relative gene expression of p53, Bax, Bcl-2 and caspase 9 was also evaluated when the cells were cultured with ConA, ConBr and ConBol. The data showed that all lectins interacted with A549 cells. Nevertheless, p53 and Bax had no significant change. Unlike, the expression of BCL-2 was down-regulated while caspase 9 was up-regulated by treatment with lectins. Concerning the pro-healing potential, the lectin isolated from the red alga Bryothamnion seafortii (BS) was tested by topical treatment of skin lesions. The data showed that BS improved the quality of healing, probably through the induction of fibroblast proliferation. Finally, the lethality test of Artemia sp. was carried out to determine the toxicity of some Diocleinae lectins. ConBr is the most toxic lectin against Artemia, while ConA is the least toxic, and ConBol, ConM, ConGr and CGL exhibit intermediate levels of toxicity.
Este trabalho avaliou o potencial biotecnolÃgico de lectinas na induÃÃo da apoptose de cÃlulas neoplÃsicas, o efeito sobre a cicatrizaÃÃo de feridas em modelo murino (in vivo) e estabeleceu o nÃvel de toxicidade sobre Artemia sp. Lectinas sÃo proteÃnas ou glicoproteÃnas distribuÃdas ubiquamente na natureza, sendo encontradas desde bactÃrias atà seres humanos. Tais proteÃnas possuem a capacidade de ligar-se seletivamente a carboidratos. Considerando que carboidratos possam atuar como mediadores da informaÃÃo biolÃgica, a sua interaÃÃo com lectinas pode desencadear algum efeito biolÃgico importante. Para verificar o papel das lectinas sobre a induÃÃo da apoptose, cÃlulas A549 (carcinoma de pulmÃo) foram cultivadas durante 1 hora juntamente com lectinas isoladas das sementes de Canavalia ensiformis (ConA), C. brasiliensis (ConBr), C. boliviana (ConBol), C. grandiflora (Cgran) e C. gladiata (CGL) marcadas com FITC. Em adiÃÃo, a expressÃo gÃnica relativa de p53, Bax, BCL-2 e caspase 9 foi tambÃm avaliada quando as cÃlulas foram cultivadas com ConA, ConBr e ConBol. Os dados mostraram que todas as lectinas intergiram com as cÃlulas A549. NÃo obstante, p53 e Bax nÃo tiveram nenhuma alteraÃÃo significativa. PorÃm, BCL-2 teve sua expressÃo diminuÃda enquanto a da caspase 9 foi aumentada pelo tratamento com as lectinas. Com relaÃÃo ao potencial pro-cicatrizante, a lectina isolada da alga vermelha Bryothamnion seafortii (BS) foi testada atravÃs do tratamento tÃpico de lesÃes cutÃneas. Os dados mostraram que BS melhora a qualidade da cicatrizaÃÃo, muito possivelmente atravÃs da induÃÃo da proliferaÃÃo de fibroblastos. Por fim, o teste de letalidade sobre Artemia sp. foi utilizado para verificar a toxicidade de algumas lectinas da subtribo Diocleinae. Os resultados mostraran que ConBr e ConA sÃo as lectinas mais tÃxica e menos tÃxica, respectivamente. ConBol, ConM, Cgran e CGL exibiram nÃveis intermediÃrios de toxicidade.
description This work evaluated the biotechnological potential of lectins in the induction of apoptosis of neoplastic cells; the effect on wound healing in a murine model (in vivo) and established the level of toxicity on Artemia sp. Lectins are proteins or glycoproteins ubiquitously distributed in nature, being found from bacteria to humans. Since carbohydrates can act as mediators of biological information, the interaction with lectins can trigger some biologically important effect. To verify the role of lectins in the induction of apoptosis, A549 cells (lung carcinoma) were grown for 1 hour with lectins isolated from seeds of Canavalia ensiformis (ConA), C. brasiliensis (ConBr), C. boliviana (ConBol), C. grandiflora (Cgran) and C. gladiata (CGL) labeled with FITC. In addition, the relative gene expression of p53, Bax, Bcl-2 and caspase 9 was also evaluated when the cells were cultured with ConA, ConBr and ConBol. The data showed that all lectins interacted with A549 cells. Nevertheless, p53 and Bax had no significant change. Unlike, the expression of BCL-2 was down-regulated while caspase 9 was up-regulated by treatment with lectins. Concerning the pro-healing potential, the lectin isolated from the red alga Bryothamnion seafortii (BS) was tested by topical treatment of skin lesions. The data showed that BS improved the quality of healing, probably through the induction of fibroblast proliferation. Finally, the lethality test of Artemia sp. was carried out to determine the toxicity of some Diocleinae lectins. ConBr is the most toxic lectin against Artemia, while ConA is the least toxic, and ConBol, ConM, ConGr and CGL exhibit intermediate levels of toxicity.
publishDate 2011
dc.date.issued.fl_str_mv 2011-11-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
status_str publishedVersion
format doctoralThesis
dc.identifier.uri.fl_str_mv http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11065
url http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11065
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.publisher.program.fl_str_mv Programa de PÃs-GraduaÃÃo em Biotecnologia (Rede Nordeste de Biotecnologia - RENORBIO)
dc.publisher.initials.fl_str_mv UFC
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFC
instname:Universidade Federal do Ceará
instacron:UFC
reponame_str Biblioteca Digital de Teses e Dissertações da UFC
collection Biblioteca Digital de Teses e Dissertações da UFC
instname_str Universidade Federal do Ceará
instacron_str UFC
institution UFC
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repository.mail.fl_str_mv mail@mail.com
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