Identification of proteins from the marine red macroalga Hypnea musciformis by proteomic analysis

Detalhes bibliográficos
Autor(a) principal: Fernando Edson Pessoa do Nascimento
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13931
Resumo: We currently live in a fierce competition in the research development of new biotechnology products, the marine environment can be seen as a source of functional biotechnological materials. Recent studies show that within the marine environment algae stand out as organisms with great biotechnological potential by having in its composition various bioactive molecules that have diverse and intense biological activities, among all algal groups in this study emphasize the of red algae, which the kind of study is red seaweed Hypnea musciformis (Wulfen). JV Lamouroux belongs to the order Gigartinales and Hypneaceae family, abundant on the coast of CearÃ. Biotechnological and scientific works are carried out with this species, in this sense, the main objective of this work is to identify and characterize proteins existing in this organism through a proteomics approach, is a new area in science that allows bioprospect a set of proteins in an organism by using analytical tools because under specific physiological conditions the biological functioning of the system can be reflected by the proteome analysis of the same, and thus the functional gene expression. Considering the lack of studies on proteomics in seaweed and in particular the carragenÃfita Hypnea musciformis, this paper aims to develop methods for separating proteins from protein extract of Hypnea musciformis and identify the proteins expressed by the organism. In this work we have established a methodology for separating proteins of this organism from a fractionated extract with ammonium sulfate and by mass spectrometry to identify proteins of some kelp, thus being of fundamental importance for the enrichment of macroalgae in the proteomics literature marine. The results of this work are consistent with our objective was the identification of three proteins by mass spectrometry using electrospray ionization type protein fraction of 20-40% and the identification of some proteins by Mascot
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spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisIdentification of proteins from the marine red macroalga Hypnea musciformis by proteomic analysisIdentificaÃÃo de proteÃnas da macroalga marinha vermelha Hypnea musciformis por anÃlise proteÃmica2014-08-29Kyria Santiago do Nascimento08671084795http://lattes.cnpq.br/4999713109630711Francisco Nascimento Pereira JÃnior01363350390http://lattes.cnpq.br/0009366744574438Celso Shiniti Nagano81850786100http://lattes.cnpq.br/773240765013509901033505358http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4270278E9Fernando Edson Pessoa do NascimentoUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em Engenharia de PescaUFCBREletrospray MALDIFracionamento protÃico Mass spectrometryElectrospray MALDIProtein fractionationRECURSOS PESQUEIROS E ENGENHARIA DE PESCAWe currently live in a fierce competition in the research development of new biotechnology products, the marine environment can be seen as a source of functional biotechnological materials. Recent studies show that within the marine environment algae stand out as organisms with great biotechnological potential by having in its composition various bioactive molecules that have diverse and intense biological activities, among all algal groups in this study emphasize the of red algae, which the kind of study is red seaweed Hypnea musciformis (Wulfen). JV Lamouroux belongs to the order Gigartinales and Hypneaceae family, abundant on the coast of CearÃ. Biotechnological and scientific works are carried out with this species, in this sense, the main objective of this work is to identify and characterize proteins existing in this organism through a proteomics approach, is a new area in science that allows bioprospect a set of proteins in an organism by using analytical tools because under specific physiological conditions the biological functioning of the system can be reflected by the proteome analysis of the same, and thus the functional gene expression. Considering the lack of studies on proteomics in seaweed and in particular the carragenÃfita Hypnea musciformis, this paper aims to develop methods for separating proteins from protein extract of Hypnea musciformis and identify the proteins expressed by the organism. In this work we have established a methodology for separating proteins of this organism from a fractionated extract with ammonium sulfate and by mass spectrometry to identify proteins of some kelp, thus being of fundamental importance for the enrichment of macroalgae in the proteomics literature marine. The results of this work are consistent with our objective was the identification of three proteins by mass spectrometry using electrospray ionization type protein fraction of 20-40% and the identification of some proteins by Mascot Atualmente vivemos em uma acirrada competiÃÃo na pesquisa de desenvolvimento de novos produtos biotecnolÃgicos, o ambiente marinho pode ser visto como uma fonte de materiais biotecnolÃgicos funcionais. Recentemente, estudos demostram que dentro do ambiente marinho as algas se destacam como organismos com grande potencial biotecnolÃgico por possuirem em sua composiÃÃo vÃrias molÃculas bioativas que apresentam diversas e intensas atividades biolÃgicas. Dentre todos os grupos algais, neste trabalho enfatizamos o das algas vermelhas, a qual a espÃcie de estudo à a alga marinha vermelha Hypnea musciformis (Wulfen). J. V. Lamouroux pertence à ordem Gigartinales e a famÃlia Hypneaceae, abundante no litoral do CearÃ. Trabalhos biotecnolÃgicos e cientÃficos sÃo realizados com esta espÃcie, neste sentido o objetivo principal deste trabalho e identificar e caracterizar proteÃnas existente neste organismo atravÃs de uma abordagem proteÃmica, que à uma Ãrea recente na ciÃncia, que permite bioprospectar um conjunto de proteÃnas presentes num organismo atravÃs do uso de ferramentas analÃticas, pois, em condiÃÃes fisiolÃgicas especÃficas, o funcionamento do sistema biolÃgico pode ser refletido por anÃlise do proteoma do mesmo, e assim a sua expressÃo gÃnica funcional. Considerando a carÃncia de estudos sobre proteÃmica em algas marinhas e em especial da carragenÃfita Hypnea musciformis, o presente trabalho tem como objetivo principal desenvolver metodologias de separaÃÃo de proteÃnas de extrato protÃico de Hypnea musciformis e identificar as proteÃnas expressas pelo organismo. Neste trabalho conseguimos estabelecer uma metodologia de separaÃÃo de proteÃnas deste organismo a partir de um extrato fracionado com sulfato de amÃnio e atravÃs de espectrometria de massas, identificar algumas proteÃnas da alga marinha. Desta forma, sendo de fundamental importÃncia para o enriquecimento na literatura de proteomica de macroalgas marinhas. Os resultados deste trabalho que estÃo de acordo com o nosso objetivo foi a identificaÃÃo de 3 proteÃnas por tÃcnica de espectrometria de massas utilizando ionizaÃÃo do tipo eletrospray da fraÃÃo protÃica 20-40% e a identificaÃÃo de algumas proteÃnas atravÃs do MascotCoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13931application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:27:08Zmail@mail.com -
dc.title.en.fl_str_mv Identification of proteins from the marine red macroalga Hypnea musciformis by proteomic analysis
dc.title.alternative.pt.fl_str_mv IdentificaÃÃo de proteÃnas da macroalga marinha vermelha Hypnea musciformis por anÃlise proteÃmica
title Identification of proteins from the marine red macroalga Hypnea musciformis by proteomic analysis
spellingShingle Identification of proteins from the marine red macroalga Hypnea musciformis by proteomic analysis
Fernando Edson Pessoa do Nascimento
Eletrospray
MALDI
Fracionamento protÃico
Mass spectrometry
Electrospray
MALDI
Protein fractionation
RECURSOS PESQUEIROS E ENGENHARIA DE PESCA
title_short Identification of proteins from the marine red macroalga Hypnea musciformis by proteomic analysis
title_full Identification of proteins from the marine red macroalga Hypnea musciformis by proteomic analysis
title_fullStr Identification of proteins from the marine red macroalga Hypnea musciformis by proteomic analysis
title_full_unstemmed Identification of proteins from the marine red macroalga Hypnea musciformis by proteomic analysis
title_sort Identification of proteins from the marine red macroalga Hypnea musciformis by proteomic analysis
author Fernando Edson Pessoa do Nascimento
author_facet Fernando Edson Pessoa do Nascimento
author_role author
dc.contributor.advisor1.fl_str_mv Kyria Santiago do Nascimento
dc.contributor.advisor1ID.fl_str_mv 08671084795
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4999713109630711
dc.contributor.referee1.fl_str_mv Francisco Nascimento Pereira JÃnior
dc.contributor.referee1ID.fl_str_mv 01363350390
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/0009366744574438
dc.contributor.referee2.fl_str_mv Celso Shiniti Nagano
dc.contributor.referee2ID.fl_str_mv 81850786100
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/7732407650135099
dc.contributor.authorID.fl_str_mv 01033505358
dc.contributor.authorLattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4270278E9
dc.contributor.author.fl_str_mv Fernando Edson Pessoa do Nascimento
contributor_str_mv Kyria Santiago do Nascimento
Francisco Nascimento Pereira JÃnior
Celso Shiniti Nagano
dc.subject.por.fl_str_mv Eletrospray
MALDI
Fracionamento protÃico
topic Eletrospray
MALDI
Fracionamento protÃico
Mass spectrometry
Electrospray
MALDI
Protein fractionation
RECURSOS PESQUEIROS E ENGENHARIA DE PESCA
dc.subject.eng.fl_str_mv Mass spectrometry
Electrospray
MALDI
Protein fractionation
dc.subject.cnpq.fl_str_mv RECURSOS PESQUEIROS E ENGENHARIA DE PESCA
dc.description.sponsorship.fl_txt_mv CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior
dc.description.abstract.por.fl_txt_mv We currently live in a fierce competition in the research development of new biotechnology products, the marine environment can be seen as a source of functional biotechnological materials. Recent studies show that within the marine environment algae stand out as organisms with great biotechnological potential by having in its composition various bioactive molecules that have diverse and intense biological activities, among all algal groups in this study emphasize the of red algae, which the kind of study is red seaweed Hypnea musciformis (Wulfen). JV Lamouroux belongs to the order Gigartinales and Hypneaceae family, abundant on the coast of CearÃ. Biotechnological and scientific works are carried out with this species, in this sense, the main objective of this work is to identify and characterize proteins existing in this organism through a proteomics approach, is a new area in science that allows bioprospect a set of proteins in an organism by using analytical tools because under specific physiological conditions the biological functioning of the system can be reflected by the proteome analysis of the same, and thus the functional gene expression. Considering the lack of studies on proteomics in seaweed and in particular the carragenÃfita Hypnea musciformis, this paper aims to develop methods for separating proteins from protein extract of Hypnea musciformis and identify the proteins expressed by the organism. In this work we have established a methodology for separating proteins of this organism from a fractionated extract with ammonium sulfate and by mass spectrometry to identify proteins of some kelp, thus being of fundamental importance for the enrichment of macroalgae in the proteomics literature marine. The results of this work are consistent with our objective was the identification of three proteins by mass spectrometry using electrospray ionization type protein fraction of 20-40% and the identification of some proteins by Mascot
Atualmente vivemos em uma acirrada competiÃÃo na pesquisa de desenvolvimento de novos produtos biotecnolÃgicos, o ambiente marinho pode ser visto como uma fonte de materiais biotecnolÃgicos funcionais. Recentemente, estudos demostram que dentro do ambiente marinho as algas se destacam como organismos com grande potencial biotecnolÃgico por possuirem em sua composiÃÃo vÃrias molÃculas bioativas que apresentam diversas e intensas atividades biolÃgicas. Dentre todos os grupos algais, neste trabalho enfatizamos o das algas vermelhas, a qual a espÃcie de estudo à a alga marinha vermelha Hypnea musciformis (Wulfen). J. V. Lamouroux pertence à ordem Gigartinales e a famÃlia Hypneaceae, abundante no litoral do CearÃ. Trabalhos biotecnolÃgicos e cientÃficos sÃo realizados com esta espÃcie, neste sentido o objetivo principal deste trabalho e identificar e caracterizar proteÃnas existente neste organismo atravÃs de uma abordagem proteÃmica, que à uma Ãrea recente na ciÃncia, que permite bioprospectar um conjunto de proteÃnas presentes num organismo atravÃs do uso de ferramentas analÃticas, pois, em condiÃÃes fisiolÃgicas especÃficas, o funcionamento do sistema biolÃgico pode ser refletido por anÃlise do proteoma do mesmo, e assim a sua expressÃo gÃnica funcional. Considerando a carÃncia de estudos sobre proteÃmica em algas marinhas e em especial da carragenÃfita Hypnea musciformis, o presente trabalho tem como objetivo principal desenvolver metodologias de separaÃÃo de proteÃnas de extrato protÃico de Hypnea musciformis e identificar as proteÃnas expressas pelo organismo. Neste trabalho conseguimos estabelecer uma metodologia de separaÃÃo de proteÃnas deste organismo a partir de um extrato fracionado com sulfato de amÃnio e atravÃs de espectrometria de massas, identificar algumas proteÃnas da alga marinha. Desta forma, sendo de fundamental importÃncia para o enriquecimento na literatura de proteomica de macroalgas marinhas. Os resultados deste trabalho que estÃo de acordo com o nosso objetivo foi a identificaÃÃo de 3 proteÃnas por tÃcnica de espectrometria de massas utilizando ionizaÃÃo do tipo eletrospray da fraÃÃo protÃica 20-40% e a identificaÃÃo de algumas proteÃnas atravÃs do Mascot
description We currently live in a fierce competition in the research development of new biotechnology products, the marine environment can be seen as a source of functional biotechnological materials. Recent studies show that within the marine environment algae stand out as organisms with great biotechnological potential by having in its composition various bioactive molecules that have diverse and intense biological activities, among all algal groups in this study emphasize the of red algae, which the kind of study is red seaweed Hypnea musciformis (Wulfen). JV Lamouroux belongs to the order Gigartinales and Hypneaceae family, abundant on the coast of CearÃ. Biotechnological and scientific works are carried out with this species, in this sense, the main objective of this work is to identify and characterize proteins existing in this organism through a proteomics approach, is a new area in science that allows bioprospect a set of proteins in an organism by using analytical tools because under specific physiological conditions the biological functioning of the system can be reflected by the proteome analysis of the same, and thus the functional gene expression. Considering the lack of studies on proteomics in seaweed and in particular the carragenÃfita Hypnea musciformis, this paper aims to develop methods for separating proteins from protein extract of Hypnea musciformis and identify the proteins expressed by the organism. In this work we have established a methodology for separating proteins of this organism from a fractionated extract with ammonium sulfate and by mass spectrometry to identify proteins of some kelp, thus being of fundamental importance for the enrichment of macroalgae in the proteomics literature marine. The results of this work are consistent with our objective was the identification of three proteins by mass spectrometry using electrospray ionization type protein fraction of 20-40% and the identification of some proteins by Mascot
publishDate 2014
dc.date.issued.fl_str_mv 2014-08-29
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
status_str publishedVersion
format masterThesis
dc.identifier.uri.fl_str_mv http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13931
url http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13931
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language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.publisher.program.fl_str_mv Programa de PÃs-GraduaÃÃo em Engenharia de Pesca
dc.publisher.initials.fl_str_mv UFC
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFC
instname:Universidade Federal do Ceará
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reponame_str Biblioteca Digital de Teses e Dissertações da UFC
collection Biblioteca Digital de Teses e Dissertações da UFC
instname_str Universidade Federal do Ceará
instacron_str UFC
institution UFC
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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