Identification phenotypic, genotypic virulence factors and characterization in isolated environmental Aeromonas spp

• Autor(a) principal: Camila MagalhÃes Silva
• Data de Publicação: 2014
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
• Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14414
• topic: Aeromonas; Testes fenotÃpicos e genotÃpicos; Meios de cultivo; Aeromonas; Phenotypic and Genotypic tests; Culture medium; ENGENHARIA DE PESCA

This study aimed to identify through polyphasic approach (phenotypic and genotypic methods) strains of Aeromonas spp. isolated from surface water and sediment from three different points of the Coco River, Cearà following the salinity gradient of the water. Sampling was conducted between October 2011 and March 2012 each collection consisted of six samples. Aeromonas strains were isolated according to the technique described in the literature Gelatin Phosphate Salt Agar (agar plus GSP 20 μg/mL ampicillin). The physico-chemical parameters (salinity, pH and temperature) of water were on a track that favored not only survival, but also the multiplication of micro-organisms in both sites. Among this sample was isolated 140 strains suspected of Aeromonas and (36.4%) 51 strains were confirmed like Aeromonas spp., wich will be identifiyed until species for fenotipic and genotipic tests. In parallel to the insulation work was done a survey of alternative culture media, where we assessed the efficiency of culture media with different compositions in the characterization of colonies of multiple species of Aeromonas. While the antimicrobial susceptibility profile of Aeromonas strains were resistant 100% penicillin (PEN) and cephalothin (CFL) and 100% antimicrobial susceptibility Amikacin (AMI) and imipenem (IMP) among the nine antimicrobials tested and generally A. caviae was the species that showed resistance to antimicrobials. Among the 27 strains tested (45.8%) were multidrug-resistant by making a multiple resistence antibiotic (MRA) > 2. Five factors of different virulence and all strains were tested had at least one of the five virulence factors tested so that different combinations of virulence factors were found in isolates from the same sample. The results of genetic analysis using primers Aero16S-F and Aero16S-F showed that all isolates identified by conventional microbiological tests were confirmed by PCR and 65.6% of the species identified by conventional biochemical tests were confirmed by Box-PCR method.