A subplacenta do preá Galea spixii Wagler, 1831
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/tede/361 |
Resumo: | The subplacenta is considered an ideal model for comparative studies of trophoblastic processes in humans. Thus, the aim of this study was made a morphological description of the development of the Spix s yellow-toothed cavy. To do this, 12 females were distributed in three groups in a proportion of 1 male to 4 females, kept in pickets of 5m2. Thereafter, vaginal cytology examinations were made daily, to verify if the females were copulated and to separate them from the other females. Then, the collection of subplacentas was made in the 15, 23, 30, 45, 53 and 55 days of gestation, by slaughtering the pregnant females using a specific anesthesic protocol. The samples were processed to standard histological techniques, cytochemistry and immunohistochemistry, and to transmission electronic microscopy. In the 15th day of gestation, the subplacenta was formed by a single cytotrophoblastic layer surrounded by a vacuolized syncytium with maternal lacunae which presented invasive characteristic in the portions apart of the cytotrophoblastic layer. In the 23th day of gestation, the subplacenta did not presented a well-defined shape, however, it was organized in lobules composed of predominantly of cytotrophoblast e its syncytium was related to the regions where maternal lacunae previously appeared. In the 30th day of gestation, the subplacenta appeared as a compact organ with a well-defined shape and with an evident mesenchymal capilarization; the lobules was composed by syncytiotrophoblast in the centre, and by a mesenchyme surrounded by cytotrophoblast. From the 45th day of gestation, the degeneration of the subplacental tissue was evident and the syncytiotrophoblast was more abundant than the cytotrophoblast; moreover, the syncytium was markedly vacuolized and presented signs of cellular death. Near to gestation term (53-54thday), the subplacenta was in an advanced degeneration stage, with evident signs of cellular death and reduction of subplacental tissue. The presence of fetal circulation was characteristic from the 23th day of gestation, evidenced by the positive reaction to vimentin; positive reaction to cytokeratin was observed during entire gestation. The proliferative activity of the subplacenta was assessed by PCNA and AgNOR procedures, and demonstrated to be higher in the beginning of the gestational period, decreasing progressively during the gestation. Ultrastructurally, the subplacenta presented cellular and syncytial trophoblastic characteristics. The development of the Spix s yellow-toothed cavy subplacenta starts around the 15th day of gestation, reaching maximum development in the 30th day and becomes necrotic in the end of gestation. Moreover, it presented an organization and structure similar to the subplacenta of other cavidae |
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A subplacenta do preá Galea spixii Wagler, 1831The subplacenta of preá Galea spixii Wagler, 1831CytotrophoblastDevelopmentSyncytiotrophoblastSubplacentaCitotrofoblastoDesenvolvimentoSinciciotrofoblastoSubplacentaCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThe subplacenta is considered an ideal model for comparative studies of trophoblastic processes in humans. Thus, the aim of this study was made a morphological description of the development of the Spix s yellow-toothed cavy. To do this, 12 females were distributed in three groups in a proportion of 1 male to 4 females, kept in pickets of 5m2. Thereafter, vaginal cytology examinations were made daily, to verify if the females were copulated and to separate them from the other females. Then, the collection of subplacentas was made in the 15, 23, 30, 45, 53 and 55 days of gestation, by slaughtering the pregnant females using a specific anesthesic protocol. The samples were processed to standard histological techniques, cytochemistry and immunohistochemistry, and to transmission electronic microscopy. In the 15th day of gestation, the subplacenta was formed by a single cytotrophoblastic layer surrounded by a vacuolized syncytium with maternal lacunae which presented invasive characteristic in the portions apart of the cytotrophoblastic layer. In the 23th day of gestation, the subplacenta did not presented a well-defined shape, however, it was organized in lobules composed of predominantly of cytotrophoblast e its syncytium was related to the regions where maternal lacunae previously appeared. In the 30th day of gestation, the subplacenta appeared as a compact organ with a well-defined shape and with an evident mesenchymal capilarization; the lobules was composed by syncytiotrophoblast in the centre, and by a mesenchyme surrounded by cytotrophoblast. From the 45th day of gestation, the degeneration of the subplacental tissue was evident and the syncytiotrophoblast was more abundant than the cytotrophoblast; moreover, the syncytium was markedly vacuolized and presented signs of cellular death. Near to gestation term (53-54thday), the subplacenta was in an advanced degeneration stage, with evident signs of cellular death and reduction of subplacental tissue. The presence of fetal circulation was characteristic from the 23th day of gestation, evidenced by the positive reaction to vimentin; positive reaction to cytokeratin was observed during entire gestation. The proliferative activity of the subplacenta was assessed by PCNA and AgNOR procedures, and demonstrated to be higher in the beginning of the gestational period, decreasing progressively during the gestation. Ultrastructurally, the subplacenta presented cellular and syncytial trophoblastic characteristics. The development of the Spix s yellow-toothed cavy subplacenta starts around the 15th day of gestation, reaching maximum development in the 30th day and becomes necrotic in the end of gestation. Moreover, it presented an organization and structure similar to the subplacenta of other cavidaeA subplacenta é considerada um modelo ideal para o estudo comparativo dos processos trofoblásticos em humanos. Dessa maneira objetivou-se descrever morfologicamente o desenvolvimento da subplacenta no preá. Para isto foram utilizadas 12 fêmeas desta espécie que foram distribuídas em três grupos numa relação de um macho para quatro fêmeas, mantidos em boxes de 5m2. Após formação dos grupos, exames de citologia vaginal eram realizados diariamente, para verificação da cópula, separando-se dos grupos as fêmeas que eram cobertas. A partir da ocorrência da cópula programaram-se as coletas das subplacentas nos dias 15, 23, 30, 45, 53, e 55 da gestação e estas foram realizadas mediante sacrifício das fêmeas gestantes com a utilização de protocolo anestésico específico. O material, então, era processado segundo técnicas para histologia convencional, citoquímica, imunohistoquímica e microscopia eletrônica de transmissão. No 15° dia de gestação observou-se que a subplacenta era constituída por uma monocamada citotrofoblástica envolta por sincício que apresentava vacúolos e lacunas maternas e que ao se afastar da camada de citotrofoblasto apresentava características invasivas. Aos 23 dias de gestação a subplacenta apresentou-se ainda sem uma forma característica definida, porém, mostrava uma conformação em lóbulos compostos predominantemente por citotrofoblasto e seu sincício esteve relacionado com as regiões onde apareciam as lacunas de origem materna. Aos 30 dias de gestação a subplacenta mostrou-se como um órgão compacto de forma definida e com a capilarização do mesênquima bastante evidente, os lóbulos eram constituídos por citotrofoblasto que envolvia o mesênquima e em seu centro possuía sinciciotrofoblasto. A partir dos 45 dias de gestação a degeneração do tecido subplacentário era evidente e a quantidade de sinciciotrofoblasto supera a de citotrofoblasto, alem disto o sincício apresenta uma grande quantidade de vacúolos e sinais de morte celular podiam ser visualizados. Aproximando-se ao termo da gestação (53 e 55 dias) a subplacenta encontrava-se em avançada degeneração e os sinais de morte celular assim como a diminuição de seu tecido eram evidentes. A presença da circulação fetal foi característica a partir do 23° dia de gestação podendo ser destacada pela reação positiva a vimentina, e a reação a citoqueratina foi positiva durante toda a gestação especialmente no citotrofoblasto. A atividade proliferativa do tecido subplacentário, avaliada pelas técnicas de PCNA e AgNOR, demonstrou ser maior no inicio da gestação e decair com o avançar desta. Ultraestruturalmente evidenciou-se as características do trofoblasto celular e sincicial. O desenvolvimento da subplacenta do preá inicia-se por volta do 15° dia de gestação, tem seu auge aos 30 dias e a termo é necrótica. Além disto, é muito semelhante a subplacenta de outros cavídeos, quanto a sua organização e estruturaConselho Nacional de Desenvolvimento Científico e TecnológicoUniversidade Federal Rural do Semi-ÁridoBRCentro de Ciências Agrárias - CCAUFERSAPrograma de Pós-Graduação em Ciência AnimalOliveira, Moacir Franco deCPF:32594950459http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703980T5Miglino, Maria AngelicaCPF:00542335859http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783016H9Favaron, Phelipe OliveiraCPF:34752716801http://lattes.cnpq.br/9687044676982334Bezerra, Ferdinando Vinícius Fernandes2016-08-15T20:31:21Z2014-10-272014-02-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfBEZERRA, Ferdinando Vinícius Fernandes. The subplacenta of preá Galea spixii Wagler, 1831. 2014. 150 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2014.https://repositorio.ufersa.edu.br/handle/tede/361porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-09-14T00:50:46Zoai:repositorio.ufersa.edu.br:tede/361Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-09-14T00:50:46Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.none.fl_str_mv |
A subplacenta do preá Galea spixii Wagler, 1831 The subplacenta of preá Galea spixii Wagler, 1831 |
title |
A subplacenta do preá Galea spixii Wagler, 1831 |
spellingShingle |
A subplacenta do preá Galea spixii Wagler, 1831 Bezerra, Ferdinando Vinícius Fernandes Cytotrophoblast Development Syncytiotrophoblast Subplacenta Citotrofoblasto Desenvolvimento Sinciciotrofoblasto Subplacenta CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
A subplacenta do preá Galea spixii Wagler, 1831 |
title_full |
A subplacenta do preá Galea spixii Wagler, 1831 |
title_fullStr |
A subplacenta do preá Galea spixii Wagler, 1831 |
title_full_unstemmed |
A subplacenta do preá Galea spixii Wagler, 1831 |
title_sort |
A subplacenta do preá Galea spixii Wagler, 1831 |
author |
Bezerra, Ferdinando Vinícius Fernandes |
author_facet |
Bezerra, Ferdinando Vinícius Fernandes |
author_role |
author |
dc.contributor.none.fl_str_mv |
Oliveira, Moacir Franco de CPF:32594950459 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4703980T5 Miglino, Maria Angelica CPF:00542335859 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783016H9 Favaron, Phelipe Oliveira CPF:34752716801 http://lattes.cnpq.br/9687044676982334 |
dc.contributor.author.fl_str_mv |
Bezerra, Ferdinando Vinícius Fernandes |
dc.subject.por.fl_str_mv |
Cytotrophoblast Development Syncytiotrophoblast Subplacenta Citotrofoblasto Desenvolvimento Sinciciotrofoblasto Subplacenta CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Cytotrophoblast Development Syncytiotrophoblast Subplacenta Citotrofoblasto Desenvolvimento Sinciciotrofoblasto Subplacenta CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The subplacenta is considered an ideal model for comparative studies of trophoblastic processes in humans. Thus, the aim of this study was made a morphological description of the development of the Spix s yellow-toothed cavy. To do this, 12 females were distributed in three groups in a proportion of 1 male to 4 females, kept in pickets of 5m2. Thereafter, vaginal cytology examinations were made daily, to verify if the females were copulated and to separate them from the other females. Then, the collection of subplacentas was made in the 15, 23, 30, 45, 53 and 55 days of gestation, by slaughtering the pregnant females using a specific anesthesic protocol. The samples were processed to standard histological techniques, cytochemistry and immunohistochemistry, and to transmission electronic microscopy. In the 15th day of gestation, the subplacenta was formed by a single cytotrophoblastic layer surrounded by a vacuolized syncytium with maternal lacunae which presented invasive characteristic in the portions apart of the cytotrophoblastic layer. In the 23th day of gestation, the subplacenta did not presented a well-defined shape, however, it was organized in lobules composed of predominantly of cytotrophoblast e its syncytium was related to the regions where maternal lacunae previously appeared. In the 30th day of gestation, the subplacenta appeared as a compact organ with a well-defined shape and with an evident mesenchymal capilarization; the lobules was composed by syncytiotrophoblast in the centre, and by a mesenchyme surrounded by cytotrophoblast. From the 45th day of gestation, the degeneration of the subplacental tissue was evident and the syncytiotrophoblast was more abundant than the cytotrophoblast; moreover, the syncytium was markedly vacuolized and presented signs of cellular death. Near to gestation term (53-54thday), the subplacenta was in an advanced degeneration stage, with evident signs of cellular death and reduction of subplacental tissue. The presence of fetal circulation was characteristic from the 23th day of gestation, evidenced by the positive reaction to vimentin; positive reaction to cytokeratin was observed during entire gestation. The proliferative activity of the subplacenta was assessed by PCNA and AgNOR procedures, and demonstrated to be higher in the beginning of the gestational period, decreasing progressively during the gestation. Ultrastructurally, the subplacenta presented cellular and syncytial trophoblastic characteristics. The development of the Spix s yellow-toothed cavy subplacenta starts around the 15th day of gestation, reaching maximum development in the 30th day and becomes necrotic in the end of gestation. Moreover, it presented an organization and structure similar to the subplacenta of other cavidae |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-27 2014-02-28 2016-08-15T20:31:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BEZERRA, Ferdinando Vinícius Fernandes. The subplacenta of preá Galea spixii Wagler, 1831. 2014. 150 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2014. https://repositorio.ufersa.edu.br/handle/tede/361 |
identifier_str_mv |
BEZERRA, Ferdinando Vinícius Fernandes. The subplacenta of preá Galea spixii Wagler, 1831. 2014. 150 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2014. |
url |
https://repositorio.ufersa.edu.br/handle/tede/361 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido BR Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido BR Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
dc.source.none.fl_str_mv |
reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
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Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
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Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
collection |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
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Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br |
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1809747474315739136 |