Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFERSA |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/tede/574 |
Resumo: | Currently, the most widely used test for assessing the seed physiological quality is the germination. However, this presents some obstacles, the largest one, is a long time to obtain results, especially for forest species. The tetrazolium test provides fast results, and this has proved to be a promising alternative for the determination of viability of this kind of seed. However, the success of the test depends on the development of an appropriate methodology for each species. Thus, this research aimed to evaluate procedures for the pre-wetting, preparation and staining jucá seeds to tetrazolium test. The experiments have been divided into two steps. As in the first trial were tested pre-wetting conditions and seed preparation, the second was dedicated to the dyeing stage, in which three concentrations of tetrazolium solution were tested (0.05, 0.075 and 0.1%) and three periods (1, 3 and 6 hours) at the temperatures of 35 to 40 ° C. The seeds were sorted into viable or non-viable according to the colorations obtained. At the same time, the germination test was conducted to compare the results with the feasibility estimates by the tetrazolium test. It was found that the mentioned test is efficient to evaluate the feasibility of jucá seeds and to implement it is recommended the scarification of the seeds with sandpaper followed by soaked paper towel for 42 hours at 25 ° C and removal of the seed coat, with subsequent immersion of the seeds into the tetrazolium solution at 0.05% for 3 hours at 35 or 40 ° C |
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Torres, Salvador BarrosBenedito, Clarisse Pereirahttp://lattes.cnpq.br/2128989556285842Camacho, Ramiro Gustavo Valerahttp://lattes.cnpq.br/1079760233135463Uchoa, Keline Sousa Albuquerquehttp://lattes.cnpq.br/801663927920482504990787480http://lattes.cnpq.br/1363783545368677http://lattes.cnpq.br/9145696078446298Carvalho, Sara Monaliza Costa2017-03-21T14:46:24Z2016-02-12CARVALHO, Sara Monaliza Costa. Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea). 2016. 70 f. Dissertação (Mestrado) - Curso de Pós-graduação em Fitotecnia, Universidade Federal Rural do Semi-Árido, Mossoró, 2016.https://repositorio.ufersa.edu.br/handle/tede/574Currently, the most widely used test for assessing the seed physiological quality is the germination. However, this presents some obstacles, the largest one, is a long time to obtain results, especially for forest species. The tetrazolium test provides fast results, and this has proved to be a promising alternative for the determination of viability of this kind of seed. However, the success of the test depends on the development of an appropriate methodology for each species. Thus, this research aimed to evaluate procedures for the pre-wetting, preparation and staining jucá seeds to tetrazolium test. The experiments have been divided into two steps. As in the first trial were tested pre-wetting conditions and seed preparation, the second was dedicated to the dyeing stage, in which three concentrations of tetrazolium solution were tested (0.05, 0.075 and 0.1%) and three periods (1, 3 and 6 hours) at the temperatures of 35 to 40 ° C. The seeds were sorted into viable or non-viable according to the colorations obtained. At the same time, the germination test was conducted to compare the results with the feasibility estimates by the tetrazolium test. It was found that the mentioned test is efficient to evaluate the feasibility of jucá seeds and to implement it is recommended the scarification of the seeds with sandpaper followed by soaked paper towel for 42 hours at 25 ° C and removal of the seed coat, with subsequent immersion of the seeds into the tetrazolium solution at 0.05% for 3 hours at 35 or 40 ° CAtualmente o teste mais utilizado para a avaliação da qualidade fisiológica de sementes é o de germinação. No entanto, este apresenta alguns entraves, sendo o maior deles o longo tempo para obtenção de resultados, principalmente para as espécies florestais. O teste de tetrazólio proporciona resultados rápidos e por isso tem se mostrado uma alternativa promissora na determinação da viabilidade de sementes dessas espécies. Todavia, o sucesso do teste depende do desenvolvimento de metodologia adequada para cada espécie. Assim, essa pesquisa teve como objetivo avaliar procedimentos destinados ao pré-umedecimento, preparo e coloração de sementes de jucá para o teste de tetrazólio. Os experimentos foram divididos em duas etapas. Enquanto no primeiro ensaio foram testadas condições de pré-umedecimento e preparo das sementes, o segundo foi dedicado à fase de coloração, onde foram testadas: três concentrações da solução de tetrazólio (0,05; 0,075 e 0,1%) e três períodos (1, 3 e 6 horas), nas temperaturas de 35 e 40 °C. As sementes foram classificadas em viáveis ou inviáveis de acordo com as colorações obtidas. Paralelamente, foi conduzido o teste de germinação para comparação dos resultados com as estimativas de viabilidade do teste de tetrazólio. Constatou-se que o teste de tetrazólio é eficiente para avaliar a viabilidade de sementes de jucá e para a sua execução recomenda-se a escarificação das sementes com lixa seguida de embebição em papel toalha por 42 horas a 25 °C e retirada do tegumento, com posterior imersão das sementes em solução de tetrazólio a 0,05% por 3 horas, a 35 ou 40 °C2016-12-05application/pdfhttp://repositorio.ufersa.edu.br/retrieve/1401/SaraMCC_DISSERT.pdf.jpgporUniversidade Federal Rural do Semi-ÁridoPrograma de Pós-Graduação em FitotecniaUFERSABrasilCC-BY-SAinfo:eu-repo/semantics/openAccessFabaceaeTeste rápidoSementes florestaisFabaceaeQuick testForest seedsCNPQ::CIENCIAS AGRARIAS::AGRONOMIATeste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea)Tetrazolium test in seeds of jucá (Libidibiaferrea (Mart. Ex Tul.) LP Queiroz var. ferrea)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Biblioteca Digital de Teses e Dissertações da UFERSAinstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAORIGINALSaraMCC_DISSERT.pdfSaraMCC_DISSERT.pdfapplication/pdf1225703https://repositorio.ufersa.edu.br//bitstream/tede/574/1/SaraMCC_DISSERT.pdf6523a91ba11bd95a902bec3898941e6bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82165https://repositorio.ufersa.edu.br//bitstream/tede/574/2/license.txtbd3efa91386c1718a7f26a329fdcb468MD52TEXTSaraMCC_DISSERT.pdf.txtSaraMCC_DISSERT.pdf.txtExtracted Texttext/plain99235https://repositorio.ufersa.edu.br//bitstream/tede/574/3/SaraMCC_DISSERT.pdf.txt2a1bf97c63c08b3b8003fafb1f133d89MD53THUMBNAILSaraMCC_DISSERT.pdf.jpgSaraMCC_DISSERT.pdf.jpgGenerated Thumbnailimage/jpeg2542https://repositorio.ufersa.edu.br//bitstream/tede/574/4/SaraMCC_DISSERT.pdf.jpg202e57542edaa1b71e68bfca190576d3MD54tede/5742022-07-11 18:43:38.529oai:repositorio.ufersa.edu.br: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Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufersa.edu.br/PUBhttp://bdtd.ufersa.edu.br/oai/requestdirecaosisbi@ufersa.edu.br|| direcaosisbi@ufersa.edu.bropendoar:2022-07-11T21:43:38Biblioteca Digital de Teses e Dissertações da UFERSA - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.por.fl_str_mv |
Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) |
dc.title.alternative.por.fl_str_mv |
Tetrazolium test in seeds of jucá (Libidibiaferrea (Mart. Ex Tul.) LP Queiroz var. ferrea) |
title |
Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) |
spellingShingle |
Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) Carvalho, Sara Monaliza Costa Fabaceae Teste rápido Sementes florestais Fabaceae Quick test Forest seeds CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) |
title_full |
Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) |
title_fullStr |
Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) |
title_full_unstemmed |
Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) |
title_sort |
Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) |
author |
Carvalho, Sara Monaliza Costa |
author_facet |
Carvalho, Sara Monaliza Costa |
author_role |
author |
dc.contributor.authorID.por.fl_str_mv |
04990787480 |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/1363783545368677 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/9145696078446298 |
dc.contributor.advisor1.fl_str_mv |
Torres, Salvador Barros |
dc.contributor.advisor-co1.fl_str_mv |
Benedito, Clarisse Pereira |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2128989556285842 |
dc.contributor.referee1.fl_str_mv |
Camacho, Ramiro Gustavo Valera |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1079760233135463 |
dc.contributor.referee2.fl_str_mv |
Uchoa, Keline Sousa Albuquerque |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/8016639279204825 |
dc.contributor.author.fl_str_mv |
Carvalho, Sara Monaliza Costa |
contributor_str_mv |
Torres, Salvador Barros Benedito, Clarisse Pereira Camacho, Ramiro Gustavo Valera Uchoa, Keline Sousa Albuquerque |
dc.subject.por.fl_str_mv |
Fabaceae Teste rápido Sementes florestais Fabaceae Quick test Forest seeds |
topic |
Fabaceae Teste rápido Sementes florestais Fabaceae Quick test Forest seeds CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Currently, the most widely used test for assessing the seed physiological quality is the germination. However, this presents some obstacles, the largest one, is a long time to obtain results, especially for forest species. The tetrazolium test provides fast results, and this has proved to be a promising alternative for the determination of viability of this kind of seed. However, the success of the test depends on the development of an appropriate methodology for each species. Thus, this research aimed to evaluate procedures for the pre-wetting, preparation and staining jucá seeds to tetrazolium test. The experiments have been divided into two steps. As in the first trial were tested pre-wetting conditions and seed preparation, the second was dedicated to the dyeing stage, in which three concentrations of tetrazolium solution were tested (0.05, 0.075 and 0.1%) and three periods (1, 3 and 6 hours) at the temperatures of 35 to 40 ° C. The seeds were sorted into viable or non-viable according to the colorations obtained. At the same time, the germination test was conducted to compare the results with the feasibility estimates by the tetrazolium test. It was found that the mentioned test is efficient to evaluate the feasibility of jucá seeds and to implement it is recommended the scarification of the seeds with sandpaper followed by soaked paper towel for 42 hours at 25 ° C and removal of the seed coat, with subsequent immersion of the seeds into the tetrazolium solution at 0.05% for 3 hours at 35 or 40 ° C |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-02-12 |
dc.date.accessioned.fl_str_mv |
2017-03-21T14:46:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARVALHO, Sara Monaliza Costa. Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea). 2016. 70 f. Dissertação (Mestrado) - Curso de Pós-graduação em Fitotecnia, Universidade Federal Rural do Semi-Árido, Mossoró, 2016. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufersa.edu.br/handle/tede/574 |
identifier_str_mv |
CARVALHO, Sara Monaliza Costa. Teste de tetrazólio em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea). 2016. 70 f. Dissertação (Mestrado) - Curso de Pós-graduação em Fitotecnia, Universidade Federal Rural do Semi-Árido, Mossoró, 2016. |
url |
https://repositorio.ufersa.edu.br/handle/tede/574 |
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por |
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por |
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CC-BY-SA info:eu-repo/semantics/openAccess |
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CC-BY-SA |
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openAccess |
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application/pdf |
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Universidade Federal Rural do Semi-Árido |
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Programa de Pós-Graduação em Fitotecnia |
dc.publisher.initials.fl_str_mv |
UFERSA |
dc.publisher.country.fl_str_mv |
Brasil |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido |
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