Percepção do uso e atividade desinfetante de libidibia ferrea

Detalhes bibliográficos
Autor(a) principal: Holanda, Jamile Rodrigues Cosme de
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
Texto Completo: https://repositorio.ufersa.edu.br/handle/prefix/1861
Resumo: The population lives exposed to a diversity of microorganisms that are present in the environment with which they coexist daily. These microorganisms may be pathogenic or not. In order to control these microorganisms on the surfaces, disinfection with chemicals is currently carried out. These, in turn, have been causing health and environmental damage, as well as the development of resistance of these microorganisms. In this way, the need arises for the use of natural alternative measures. Since Libidibia ferrea is a tree with great medicinal potential, it is sought to analyze the inhibitory properties of L. ferrea leaves and decoct, and the phytochemical components, as well as the communities' knowledge and acceptance of this alternative disinfectant. effectiveness as a disinfectant. For this, the following methodologies were used: disc diffusion technique and growth curve under the action of juca to evaluate the antimicrobial activity; disinfectant evaluation test on plastic surfaces, stainless steel and ceramics and phytochemical prospecting, as well as the application of the product acceptability test and the questionnaire to evaluate the knowledge about the use of jucá in the rural communities of the municipality of Mossoró / RN. A statistical analysis was given by the SigmaPlot program, by One-Way ANOVA and One-Way ANOVA RM followed by Tukey, Kruskal-Wallis and Friedman. For the sensorial analysis the Wilconxon was used, being the values of 0,05 signified. As a result, in the well diffusion technique the sensitivity of S. aureus and E. feacalis to the decoction and for the extract P. aeruginosa, Micrococcus spp., Corynebacterium spp., S. Typhirium, S. aureus and E. feacalis . As for the growth curve evaluated after 24 hours, this showed a reduction of the absorbance value for the extract to P. aeruginoa, Corynebacterium spp., S. Typhirium and E. feacalis, being that for the decoction only the latter. In the test of the disinfectant action on the surfaces it was verified that there was a bacterial reduction for the decoction as for the extract considering the negative control in all the surfaces, except for S. Typhimurium in relation to the steel. Phytochemistry showed the presence of flavonoids, saponins, tannins, coumarins, steroids and phenolic compounds. It was also observed that in all the questionnaires evaluated there is a greater acceptance of disinfectant based on L. ferrea leaves. In this way it can be affirmed that the jucá-based disinfectant is an effective natural alternative and well accepted by the population
id UFER_a3d11813af549157394aaeeb86ccfe8e
oai_identifier_str oai:repositorio.ufersa.edu.br:prefix/1861
network_acronym_str UFER
network_name_str Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
repository_id_str
spelling Percepção do uso e atividade desinfetante de libidibia ferreaDesinfecçãoExtrato vegetaisTeste de sensibilidade microbianaAnálise sensorialDecoctoDisinfectionPlant ExtractMicrobial Sensitivity TestSensory AnalysisDecoctCNPQ::CIENCIAS AMBIENTAIS::MEIO AMBIENTEThe population lives exposed to a diversity of microorganisms that are present in the environment with which they coexist daily. These microorganisms may be pathogenic or not. In order to control these microorganisms on the surfaces, disinfection with chemicals is currently carried out. These, in turn, have been causing health and environmental damage, as well as the development of resistance of these microorganisms. In this way, the need arises for the use of natural alternative measures. Since Libidibia ferrea is a tree with great medicinal potential, it is sought to analyze the inhibitory properties of L. ferrea leaves and decoct, and the phytochemical components, as well as the communities' knowledge and acceptance of this alternative disinfectant. effectiveness as a disinfectant. For this, the following methodologies were used: disc diffusion technique and growth curve under the action of juca to evaluate the antimicrobial activity; disinfectant evaluation test on plastic surfaces, stainless steel and ceramics and phytochemical prospecting, as well as the application of the product acceptability test and the questionnaire to evaluate the knowledge about the use of jucá in the rural communities of the municipality of Mossoró / RN. A statistical analysis was given by the SigmaPlot program, by One-Way ANOVA and One-Way ANOVA RM followed by Tukey, Kruskal-Wallis and Friedman. For the sensorial analysis the Wilconxon was used, being the values of 0,05 signified. As a result, in the well diffusion technique the sensitivity of S. aureus and E. feacalis to the decoction and for the extract P. aeruginosa, Micrococcus spp., Corynebacterium spp., S. Typhirium, S. aureus and E. feacalis . As for the growth curve evaluated after 24 hours, this showed a reduction of the absorbance value for the extract to P. aeruginoa, Corynebacterium spp., S. Typhirium and E. feacalis, being that for the decoction only the latter. In the test of the disinfectant action on the surfaces it was verified that there was a bacterial reduction for the decoction as for the extract considering the negative control in all the surfaces, except for S. Typhimurium in relation to the steel. Phytochemistry showed the presence of flavonoids, saponins, tannins, coumarins, steroids and phenolic compounds. It was also observed that in all the questionnaires evaluated there is a greater acceptance of disinfectant based on L. ferrea leaves. In this way it can be affirmed that the jucá-based disinfectant is an effective natural alternative and well accepted by the populationA população vive exposta a uma diversidade de micro-organismos que estão presentes no meio ambiente com os quais convivem diariamente. Podendo tais microorganismos ser patogênicos ou não. A fim de controlar estes micro-organismos nas superfícies, realiza-se a desinfecção, com produtos químicos. Estes, por sua vez, vêm ocasionando agravos ao meio ambiente e saúde, bem como proporcionando o desenvolvimento da resistência destes micro-organismos. Desta forma, surge a necessidade do emprego de medidas alternativas naturais. Visto que a Libidibia ferrea é uma árvore que tem grande potencial medicinal, busca-se analisar as propriedades inibitórias do extrato e decocto das folhas da L. ferrea, e os componentes fitoquímicos, como também o conhecimento e aceitação das comunidades sobre esse desinfetante alternativo e sua eficácia como desinfetante. Para tal, adotou-se as seguintes metodologias: técnica de disco difusão e curva de crescimento sob a ação do jucá para a avaliação da atividade antimicrobiana; teste de avaliação desinfetante nas superfícies plásticas, aço inox e cerâmica e prospecção fitoquímica, bem como, a aplicação do teste de aceitabilidade do produto e do questionário para avaliar as formas de conhecimento sobre o uso do jucá, nas comunidades rurais de tradição do município de Mossoró/RN. A analise estatística deu-se pelo programa SigmaPlot, por One-Way ANOVA e One-Way ANOVA RM seguidos de Tukey, Kruskal-Wallis e Friedman. Para a analise sensorial foi usado o Wilconxon, sendo os valores de p>0,05 significatidos. Obtendo como resultado na técnica de disco difusão em poços a sensibilidade da S. aureus e E. feacalis ao decocto e para o extrato a P. aeruginosa, Micrococcus spp., Corynebacterium spp., S. Typhirium, S. aureus e E. feacalis. Quanto a curva de crescimento avaliada após as 24 horas, essa demostrou uma redução do valor de absorbância para o extrato a P. aeruginoa, Corynebacterium spp., S. Typhirium e E. feacalis, para o decocto somente a ultima. No teste da ação desinfetante nas superfícies comprovou-se que houve uma redução bacteriana para o decocto como para o extrato considerando o controle negativo em todas as superfícies, com exceção da S. Typhimurium em relação ao aço. Quanto à fitoquímica evidenciou-se a presença de flavonóides, saponinas, taninos, cumarinas, esteroides e compostos fenólicos. Observou-se também que em todos os questionários avaliados há uma maior aceitação do desinfetante a base das folhas da L. ferrea. Desta forma pode-se afirmar que o desinfetante a base de jucá é uma alternativa natural eficaz e bem aceita pela populaçãoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESUniversidade Federal Rural do Semi-ÁridoBrasilCentro de Ciências Agrárias - CCAUFERSAPrograma de Pós-Graduação em Ambiente, Tecnologia e SociedadeFeijó, Francisco Marlon Carneiro41688597387http://lattes.cnpq.br/7436750766676260Leite, Ricardo Henrique de Lima52264343400http://lattes.cnpq.br/3801476460958779Alves, Nilza Dutra42583780387http://lattes.cnpq.br/5897477356455243Coelho, Wesley Adson Costa01080935495http://lattes.cnpq.br/8362491439074916Holanda, Jamile Rodrigues Cosme de2019-06-17T11:53:36Z2019-06-172019-06-17T11:53:36Z2019-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfCitação com autor incluído no texto: Holanda (2019) Citação com autor não incluído no texto: (HOLANDA, 2019)https://repositorio.ufersa.edu.br/handle/prefix/1861porHOLANDA, Jamile Rodrigues Cosme de. Percepção do uso e atividade desinfetante de libidibia ferrea. 2019. 90 f. Dissertação (Mestrado em Ambiente, Tecnologia e Sociedade), Universidade Federal Rural do Semi-Árido, Mossoró, 2019.CC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-10-30T20:29:08Zoai:repositorio.ufersa.edu.br:prefix/1861Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-10-30T20:29:08Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false
dc.title.none.fl_str_mv Percepção do uso e atividade desinfetante de libidibia ferrea
title Percepção do uso e atividade desinfetante de libidibia ferrea
spellingShingle Percepção do uso e atividade desinfetante de libidibia ferrea
Holanda, Jamile Rodrigues Cosme de
Desinfecção
Extrato vegetais
Teste de sensibilidade microbiana
Análise sensorial
Decocto
Disinfection
Plant Extract
Microbial Sensitivity Test
Sensory Analysis
Decoct
CNPQ::CIENCIAS AMBIENTAIS::MEIO AMBIENTE
title_short Percepção do uso e atividade desinfetante de libidibia ferrea
title_full Percepção do uso e atividade desinfetante de libidibia ferrea
title_fullStr Percepção do uso e atividade desinfetante de libidibia ferrea
title_full_unstemmed Percepção do uso e atividade desinfetante de libidibia ferrea
title_sort Percepção do uso e atividade desinfetante de libidibia ferrea
author Holanda, Jamile Rodrigues Cosme de
author_facet Holanda, Jamile Rodrigues Cosme de
author_role author
dc.contributor.none.fl_str_mv Feijó, Francisco Marlon Carneiro
41688597387
http://lattes.cnpq.br/7436750766676260
Leite, Ricardo Henrique de Lima
52264343400
http://lattes.cnpq.br/3801476460958779
Alves, Nilza Dutra
42583780387
http://lattes.cnpq.br/5897477356455243
Coelho, Wesley Adson Costa
01080935495
http://lattes.cnpq.br/8362491439074916
dc.contributor.author.fl_str_mv Holanda, Jamile Rodrigues Cosme de
dc.subject.por.fl_str_mv Desinfecção
Extrato vegetais
Teste de sensibilidade microbiana
Análise sensorial
Decocto
Disinfection
Plant Extract
Microbial Sensitivity Test
Sensory Analysis
Decoct
CNPQ::CIENCIAS AMBIENTAIS::MEIO AMBIENTE
topic Desinfecção
Extrato vegetais
Teste de sensibilidade microbiana
Análise sensorial
Decocto
Disinfection
Plant Extract
Microbial Sensitivity Test
Sensory Analysis
Decoct
CNPQ::CIENCIAS AMBIENTAIS::MEIO AMBIENTE
description The population lives exposed to a diversity of microorganisms that are present in the environment with which they coexist daily. These microorganisms may be pathogenic or not. In order to control these microorganisms on the surfaces, disinfection with chemicals is currently carried out. These, in turn, have been causing health and environmental damage, as well as the development of resistance of these microorganisms. In this way, the need arises for the use of natural alternative measures. Since Libidibia ferrea is a tree with great medicinal potential, it is sought to analyze the inhibitory properties of L. ferrea leaves and decoct, and the phytochemical components, as well as the communities' knowledge and acceptance of this alternative disinfectant. effectiveness as a disinfectant. For this, the following methodologies were used: disc diffusion technique and growth curve under the action of juca to evaluate the antimicrobial activity; disinfectant evaluation test on plastic surfaces, stainless steel and ceramics and phytochemical prospecting, as well as the application of the product acceptability test and the questionnaire to evaluate the knowledge about the use of jucá in the rural communities of the municipality of Mossoró / RN. A statistical analysis was given by the SigmaPlot program, by One-Way ANOVA and One-Way ANOVA RM followed by Tukey, Kruskal-Wallis and Friedman. For the sensorial analysis the Wilconxon was used, being the values of 0,05 signified. As a result, in the well diffusion technique the sensitivity of S. aureus and E. feacalis to the decoction and for the extract P. aeruginosa, Micrococcus spp., Corynebacterium spp., S. Typhirium, S. aureus and E. feacalis . As for the growth curve evaluated after 24 hours, this showed a reduction of the absorbance value for the extract to P. aeruginoa, Corynebacterium spp., S. Typhirium and E. feacalis, being that for the decoction only the latter. In the test of the disinfectant action on the surfaces it was verified that there was a bacterial reduction for the decoction as for the extract considering the negative control in all the surfaces, except for S. Typhimurium in relation to the steel. Phytochemistry showed the presence of flavonoids, saponins, tannins, coumarins, steroids and phenolic compounds. It was also observed that in all the questionnaires evaluated there is a greater acceptance of disinfectant based on L. ferrea leaves. In this way it can be affirmed that the jucá-based disinfectant is an effective natural alternative and well accepted by the population
publishDate 2019
dc.date.none.fl_str_mv 2019-06-17T11:53:36Z
2019-06-17
2019-06-17T11:53:36Z
2019-02-27
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv Citação com autor incluído no texto: Holanda (2019) Citação com autor não incluído no texto: (HOLANDA, 2019)
https://repositorio.ufersa.edu.br/handle/prefix/1861
identifier_str_mv Citação com autor incluído no texto: Holanda (2019) Citação com autor não incluído no texto: (HOLANDA, 2019)
url https://repositorio.ufersa.edu.br/handle/prefix/1861
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv HOLANDA, Jamile Rodrigues Cosme de. Percepção do uso e atividade desinfetante de libidibia ferrea. 2019. 90 f. Dissertação (Mestrado em Ambiente, Tecnologia e Sociedade), Universidade Federal Rural do Semi-Árido, Mossoró, 2019.
dc.rights.driver.fl_str_mv CC-BY-SA
info:eu-repo/semantics/openAccess
rights_invalid_str_mv CC-BY-SA
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
Brasil
Centro de Ciências Agrárias - CCA
UFERSA
Programa de Pós-Graduação em Ambiente, Tecnologia e Sociedade
publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
Brasil
Centro de Ciências Agrárias - CCA
UFERSA
Programa de Pós-Graduação em Ambiente, Tecnologia e Sociedade
dc.source.none.fl_str_mv reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
instname:Universidade Federal Rural do Semi-Árido (UFERSA)
instacron:UFERSA
instname_str Universidade Federal Rural do Semi-Árido (UFERSA)
instacron_str UFERSA
institution UFERSA
reponame_str Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
collection Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
repository.name.fl_str_mv Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)
repository.mail.fl_str_mv repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br
_version_ 1809747493440716800