Avaliação da transfusão sanguínea homóloga em ovinos

Detalhes bibliográficos
Autor(a) principal: Sousa, Rejane dos Santos
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
Texto Completo: https://repositorio.ufersa.edu.br/handle/tede/334
Resumo: This study evaluated the clinical, hematological, biochemical and blood gas responses and the oxidative stress in sheep submitted to hiperacute anemia and subsequently underwent to homologous transfusion with whole blood, either fresh, or stored for 15 or 35 days. Eighteen adult Santa Inez crossbred sheep, males and females, were used, weighing on average 52kg. The animals were submitted to a single phlebotomy to remove 40% of blood volume and equally divided into three experimental groups: G0 - animals receiving fresh blood; G15 receiving blood stored for 15 days; and G35 - receiving blood stored for 35 days. The animals were submitted to clinical examination and blood collection 24 h after induction of anemia (T0), 30 minutes after transfusion (T30), six, twelve, twenty-four, forty-eight, seventy-two and ninety-six hours post-transfusion (T6, T12, T24, T48, T72 and T96, respectively), and eight and sixteen days post-transfusion (T8d and T16d, respectively). The blood bags stored for 35 days showed an increase in K, pCO2, pO2, lactate, plasma hemoglobin and decreased plasma pH, sodium and leukocytes. The sheep transfused from all groups had increased GV, red cell count and total hemoglobin in the T30. The animals of the G35 had higher plasma hemoglobin in T12, a significant decrease in blood pH indicating a mild metabolic acidosis on T96. With respect to oxidative stress, was observed a decreased on catalase values of the G35 at T30, T6, T12 and T24, suggesting the occurrence of hemolysis witch was supported by the concomitant increase in the total bilirubin values at the same periods. Animals that received blood stored for 35 days showed higher alteration on hematological, blood gas, biochemical and oxidative stress parameters
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spelling Avaliação da transfusão sanguínea homóloga em ovinosEvaluation of homologous blood transfusion in sheepRed blood cellsOxidative stressBlood gas analysisHemáciasestresse oxidativoAnálises hemogasométricaCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThis study evaluated the clinical, hematological, biochemical and blood gas responses and the oxidative stress in sheep submitted to hiperacute anemia and subsequently underwent to homologous transfusion with whole blood, either fresh, or stored for 15 or 35 days. Eighteen adult Santa Inez crossbred sheep, males and females, were used, weighing on average 52kg. The animals were submitted to a single phlebotomy to remove 40% of blood volume and equally divided into three experimental groups: G0 - animals receiving fresh blood; G15 receiving blood stored for 15 days; and G35 - receiving blood stored for 35 days. The animals were submitted to clinical examination and blood collection 24 h after induction of anemia (T0), 30 minutes after transfusion (T30), six, twelve, twenty-four, forty-eight, seventy-two and ninety-six hours post-transfusion (T6, T12, T24, T48, T72 and T96, respectively), and eight and sixteen days post-transfusion (T8d and T16d, respectively). The blood bags stored for 35 days showed an increase in K, pCO2, pO2, lactate, plasma hemoglobin and decreased plasma pH, sodium and leukocytes. The sheep transfused from all groups had increased GV, red cell count and total hemoglobin in the T30. The animals of the G35 had higher plasma hemoglobin in T12, a significant decrease in blood pH indicating a mild metabolic acidosis on T96. With respect to oxidative stress, was observed a decreased on catalase values of the G35 at T30, T6, T12 and T24, suggesting the occurrence of hemolysis witch was supported by the concomitant increase in the total bilirubin values at the same periods. Animals that received blood stored for 35 days showed higher alteration on hematological, blood gas, biochemical and oxidative stress parametersO presente trabalho objetivou avaliar as respostas clínicas, hematológicas, bioquímicas, hemogasométricas e o estresse oxidativo de ovinos induzidos à anemia superaguda e transfundidos com sangue total fresco ou armazenado por dois diferentes períodos (15 e 35 dias). Foram utilizados 18 ovinos, machos e fêmeas, com idade entre 3 e 4 anos, pesando em media 52kg. Os animais foram submetidos a uma única flebotomia para retirada de 40% do volume sanguíneo e divididos em três grupos experimentais, sendo o G0 composto por animais que receberam sangue fresco, G15 e G35 animais que receberam sangue armazenado em bolsas CPDA-1 por 15 e 35 dias, respectivamente. Foi realizado exame clínico e coleta de amostras de sangue 24 horas pós-indução da anemia (T0), 30 minutos pós-transfusão (T30), seis, 12, 24, 48, 72 e 96 horas após à transfusão (T6, T12, T24, T48, T72, T96, respectivamente) e oito e dezesseis dias após à transfusão (T8d e T16d, respectivamente). O sangue armazenado por 35 dias apresentou aumento do potássio, pCO2, pO2, lactato, hemoglobina plasmática e diminuição do pH, sódio e leucócitos. Ovinos transfundidos no T30 apresentaram aumento significativo do VG, hemácias e hemoglobina total. Os animais do G35 apresentaram maiores valores de hemoglobina plasmática no T12 e diminuição do pH sanguíneo, caracterizando leve acidemia metabólica no T96. Com relação ao estresse oxidativo o G35 apresentou redução da catalase no T30, T6, T12 e T24, indicando a ocorrência de hemólise, o que foi corroborado pelo aumento concomitante da bilirrubina. Os animais que receberam sangue armazenado por 35 dias apresentaram maiores alterações hematológicas, hemogasométricas, bioquímicas e oxidativasConselho Nacional de Desenvolvimento Científico e TecnológicoUniversidade Federal Rural do Semi-ÁridoBRCentro de Ciências Agrárias - CCAUFERSAPrograma de Pós-Graduação em Ciência AnimalBarrêto Júnior, Raimundo AlvesCPF:43214366387http://lattes.cnpq.br/0516971232838494Paula, Valéria Veras deCPF:36261300372http://lattes.cnpq.br/9083821440600669Ortolani, Enrico LippiCPF:00330026810http://lattes.cnpq.br/5614582531767994Sousa, Rejane dos Santos2016-08-15T20:31:08Z2013-12-022012-07-04info:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfSOUSA, Rejane dos Santos. Evaluation of homologous blood transfusion in sheep. 2012. 169 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2012.https://repositorio.ufersa.edu.br/handle/tede/334porinfo:eu-repo/semantics/openAccessCC-BY-SAreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-09-14T00:35:42Zoai:repositorio.ufersa.edu.br:tede/334Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-09-14T00:35:42Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false
dc.title.none.fl_str_mv Avaliação da transfusão sanguínea homóloga em ovinos
Evaluation of homologous blood transfusion in sheep
title Avaliação da transfusão sanguínea homóloga em ovinos
spellingShingle Avaliação da transfusão sanguínea homóloga em ovinos
Sousa, Rejane dos Santos
Red blood cells
Oxidative stress
Blood gas analysis
Hemácias
estresse oxidativo
Análises hemogasométrica
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
title_short Avaliação da transfusão sanguínea homóloga em ovinos
title_full Avaliação da transfusão sanguínea homóloga em ovinos
title_fullStr Avaliação da transfusão sanguínea homóloga em ovinos
title_full_unstemmed Avaliação da transfusão sanguínea homóloga em ovinos
title_sort Avaliação da transfusão sanguínea homóloga em ovinos
author Sousa, Rejane dos Santos
author_facet Sousa, Rejane dos Santos
author_role author
dc.contributor.none.fl_str_mv Barrêto Júnior, Raimundo Alves
CPF:43214366387
http://lattes.cnpq.br/0516971232838494
Paula, Valéria Veras de
CPF:36261300372
http://lattes.cnpq.br/9083821440600669
Ortolani, Enrico Lippi
CPF:00330026810
http://lattes.cnpq.br/5614582531767994
dc.contributor.author.fl_str_mv Sousa, Rejane dos Santos
dc.subject.por.fl_str_mv Red blood cells
Oxidative stress
Blood gas analysis
Hemácias
estresse oxidativo
Análises hemogasométrica
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
topic Red blood cells
Oxidative stress
Blood gas analysis
Hemácias
estresse oxidativo
Análises hemogasométrica
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
description This study evaluated the clinical, hematological, biochemical and blood gas responses and the oxidative stress in sheep submitted to hiperacute anemia and subsequently underwent to homologous transfusion with whole blood, either fresh, or stored for 15 or 35 days. Eighteen adult Santa Inez crossbred sheep, males and females, were used, weighing on average 52kg. The animals were submitted to a single phlebotomy to remove 40% of blood volume and equally divided into three experimental groups: G0 - animals receiving fresh blood; G15 receiving blood stored for 15 days; and G35 - receiving blood stored for 35 days. The animals were submitted to clinical examination and blood collection 24 h after induction of anemia (T0), 30 minutes after transfusion (T30), six, twelve, twenty-four, forty-eight, seventy-two and ninety-six hours post-transfusion (T6, T12, T24, T48, T72 and T96, respectively), and eight and sixteen days post-transfusion (T8d and T16d, respectively). The blood bags stored for 35 days showed an increase in K, pCO2, pO2, lactate, plasma hemoglobin and decreased plasma pH, sodium and leukocytes. The sheep transfused from all groups had increased GV, red cell count and total hemoglobin in the T30. The animals of the G35 had higher plasma hemoglobin in T12, a significant decrease in blood pH indicating a mild metabolic acidosis on T96. With respect to oxidative stress, was observed a decreased on catalase values of the G35 at T30, T6, T12 and T24, suggesting the occurrence of hemolysis witch was supported by the concomitant increase in the total bilirubin values at the same periods. Animals that received blood stored for 35 days showed higher alteration on hematological, blood gas, biochemical and oxidative stress parameters
publishDate 2012
dc.date.none.fl_str_mv 2012-07-04
2013-12-02
2016-08-15T20:31:08Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SOUSA, Rejane dos Santos. Evaluation of homologous blood transfusion in sheep. 2012. 169 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2012.
https://repositorio.ufersa.edu.br/handle/tede/334
identifier_str_mv SOUSA, Rejane dos Santos. Evaluation of homologous blood transfusion in sheep. 2012. 169 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2012.
url https://repositorio.ufersa.edu.br/handle/tede/334
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
CC-BY-SA
eu_rights_str_mv openAccess
rights_invalid_str_mv CC-BY-SA
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
BR
Centro de Ciências Agrárias - CCA
UFERSA
Programa de Pós-Graduação em Ciência Animal
publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
BR
Centro de Ciências Agrárias - CCA
UFERSA
Programa de Pós-Graduação em Ciência Animal
dc.source.none.fl_str_mv reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
instname:Universidade Federal Rural do Semi-Árido (UFERSA)
instacron:UFERSA
instname_str Universidade Federal Rural do Semi-Árido (UFERSA)
instacron_str UFERSA
institution UFERSA
reponame_str Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
collection Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)
repository.name.fl_str_mv Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)
repository.mail.fl_str_mv repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br
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