Avaliação da transfusão sanguínea homóloga em ovinos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/tede/334 |
Resumo: | This study evaluated the clinical, hematological, biochemical and blood gas responses and the oxidative stress in sheep submitted to hiperacute anemia and subsequently underwent to homologous transfusion with whole blood, either fresh, or stored for 15 or 35 days. Eighteen adult Santa Inez crossbred sheep, males and females, were used, weighing on average 52kg. The animals were submitted to a single phlebotomy to remove 40% of blood volume and equally divided into three experimental groups: G0 - animals receiving fresh blood; G15 receiving blood stored for 15 days; and G35 - receiving blood stored for 35 days. The animals were submitted to clinical examination and blood collection 24 h after induction of anemia (T0), 30 minutes after transfusion (T30), six, twelve, twenty-four, forty-eight, seventy-two and ninety-six hours post-transfusion (T6, T12, T24, T48, T72 and T96, respectively), and eight and sixteen days post-transfusion (T8d and T16d, respectively). The blood bags stored for 35 days showed an increase in K, pCO2, pO2, lactate, plasma hemoglobin and decreased plasma pH, sodium and leukocytes. The sheep transfused from all groups had increased GV, red cell count and total hemoglobin in the T30. The animals of the G35 had higher plasma hemoglobin in T12, a significant decrease in blood pH indicating a mild metabolic acidosis on T96. With respect to oxidative stress, was observed a decreased on catalase values of the G35 at T30, T6, T12 and T24, suggesting the occurrence of hemolysis witch was supported by the concomitant increase in the total bilirubin values at the same periods. Animals that received blood stored for 35 days showed higher alteration on hematological, blood gas, biochemical and oxidative stress parameters |
id |
UFER_a9e0705ac8bd9d22b2101376a8081296 |
---|---|
oai_identifier_str |
oai:repositorio.ufersa.edu.br:tede/334 |
network_acronym_str |
UFER |
network_name_str |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
repository_id_str |
|
spelling |
Avaliação da transfusão sanguínea homóloga em ovinosEvaluation of homologous blood transfusion in sheepRed blood cellsOxidative stressBlood gas analysisHemáciasestresse oxidativoAnálises hemogasométricaCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThis study evaluated the clinical, hematological, biochemical and blood gas responses and the oxidative stress in sheep submitted to hiperacute anemia and subsequently underwent to homologous transfusion with whole blood, either fresh, or stored for 15 or 35 days. Eighteen adult Santa Inez crossbred sheep, males and females, were used, weighing on average 52kg. The animals were submitted to a single phlebotomy to remove 40% of blood volume and equally divided into three experimental groups: G0 - animals receiving fresh blood; G15 receiving blood stored for 15 days; and G35 - receiving blood stored for 35 days. The animals were submitted to clinical examination and blood collection 24 h after induction of anemia (T0), 30 minutes after transfusion (T30), six, twelve, twenty-four, forty-eight, seventy-two and ninety-six hours post-transfusion (T6, T12, T24, T48, T72 and T96, respectively), and eight and sixteen days post-transfusion (T8d and T16d, respectively). The blood bags stored for 35 days showed an increase in K, pCO2, pO2, lactate, plasma hemoglobin and decreased plasma pH, sodium and leukocytes. The sheep transfused from all groups had increased GV, red cell count and total hemoglobin in the T30. The animals of the G35 had higher plasma hemoglobin in T12, a significant decrease in blood pH indicating a mild metabolic acidosis on T96. With respect to oxidative stress, was observed a decreased on catalase values of the G35 at T30, T6, T12 and T24, suggesting the occurrence of hemolysis witch was supported by the concomitant increase in the total bilirubin values at the same periods. Animals that received blood stored for 35 days showed higher alteration on hematological, blood gas, biochemical and oxidative stress parametersO presente trabalho objetivou avaliar as respostas clínicas, hematológicas, bioquímicas, hemogasométricas e o estresse oxidativo de ovinos induzidos à anemia superaguda e transfundidos com sangue total fresco ou armazenado por dois diferentes períodos (15 e 35 dias). Foram utilizados 18 ovinos, machos e fêmeas, com idade entre 3 e 4 anos, pesando em media 52kg. Os animais foram submetidos a uma única flebotomia para retirada de 40% do volume sanguíneo e divididos em três grupos experimentais, sendo o G0 composto por animais que receberam sangue fresco, G15 e G35 animais que receberam sangue armazenado em bolsas CPDA-1 por 15 e 35 dias, respectivamente. Foi realizado exame clínico e coleta de amostras de sangue 24 horas pós-indução da anemia (T0), 30 minutos pós-transfusão (T30), seis, 12, 24, 48, 72 e 96 horas após à transfusão (T6, T12, T24, T48, T72, T96, respectivamente) e oito e dezesseis dias após à transfusão (T8d e T16d, respectivamente). O sangue armazenado por 35 dias apresentou aumento do potássio, pCO2, pO2, lactato, hemoglobina plasmática e diminuição do pH, sódio e leucócitos. Ovinos transfundidos no T30 apresentaram aumento significativo do VG, hemácias e hemoglobina total. Os animais do G35 apresentaram maiores valores de hemoglobina plasmática no T12 e diminuição do pH sanguíneo, caracterizando leve acidemia metabólica no T96. Com relação ao estresse oxidativo o G35 apresentou redução da catalase no T30, T6, T12 e T24, indicando a ocorrência de hemólise, o que foi corroborado pelo aumento concomitante da bilirrubina. Os animais que receberam sangue armazenado por 35 dias apresentaram maiores alterações hematológicas, hemogasométricas, bioquímicas e oxidativasConselho Nacional de Desenvolvimento Científico e TecnológicoUniversidade Federal Rural do Semi-ÁridoBRCentro de Ciências Agrárias - CCAUFERSAPrograma de Pós-Graduação em Ciência AnimalBarrêto Júnior, Raimundo AlvesCPF:43214366387http://lattes.cnpq.br/0516971232838494Paula, Valéria Veras deCPF:36261300372http://lattes.cnpq.br/9083821440600669Ortolani, Enrico LippiCPF:00330026810http://lattes.cnpq.br/5614582531767994Sousa, Rejane dos Santos2016-08-15T20:31:08Z2013-12-022012-07-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSOUSA, Rejane dos Santos. Evaluation of homologous blood transfusion in sheep. 2012. 169 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2012.https://repositorio.ufersa.edu.br/handle/tede/334porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-09-14T00:35:42Zoai:repositorio.ufersa.edu.br:tede/334Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-09-14T00:35:42Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.none.fl_str_mv |
Avaliação da transfusão sanguínea homóloga em ovinos Evaluation of homologous blood transfusion in sheep |
title |
Avaliação da transfusão sanguínea homóloga em ovinos |
spellingShingle |
Avaliação da transfusão sanguínea homóloga em ovinos Sousa, Rejane dos Santos Red blood cells Oxidative stress Blood gas analysis Hemácias estresse oxidativo Análises hemogasométrica CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Avaliação da transfusão sanguínea homóloga em ovinos |
title_full |
Avaliação da transfusão sanguínea homóloga em ovinos |
title_fullStr |
Avaliação da transfusão sanguínea homóloga em ovinos |
title_full_unstemmed |
Avaliação da transfusão sanguínea homóloga em ovinos |
title_sort |
Avaliação da transfusão sanguínea homóloga em ovinos |
author |
Sousa, Rejane dos Santos |
author_facet |
Sousa, Rejane dos Santos |
author_role |
author |
dc.contributor.none.fl_str_mv |
Barrêto Júnior, Raimundo Alves CPF:43214366387 http://lattes.cnpq.br/0516971232838494 Paula, Valéria Veras de CPF:36261300372 http://lattes.cnpq.br/9083821440600669 Ortolani, Enrico Lippi CPF:00330026810 http://lattes.cnpq.br/5614582531767994 |
dc.contributor.author.fl_str_mv |
Sousa, Rejane dos Santos |
dc.subject.por.fl_str_mv |
Red blood cells Oxidative stress Blood gas analysis Hemácias estresse oxidativo Análises hemogasométrica CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Red blood cells Oxidative stress Blood gas analysis Hemácias estresse oxidativo Análises hemogasométrica CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
This study evaluated the clinical, hematological, biochemical and blood gas responses and the oxidative stress in sheep submitted to hiperacute anemia and subsequently underwent to homologous transfusion with whole blood, either fresh, or stored for 15 or 35 days. Eighteen adult Santa Inez crossbred sheep, males and females, were used, weighing on average 52kg. The animals were submitted to a single phlebotomy to remove 40% of blood volume and equally divided into three experimental groups: G0 - animals receiving fresh blood; G15 receiving blood stored for 15 days; and G35 - receiving blood stored for 35 days. The animals were submitted to clinical examination and blood collection 24 h after induction of anemia (T0), 30 minutes after transfusion (T30), six, twelve, twenty-four, forty-eight, seventy-two and ninety-six hours post-transfusion (T6, T12, T24, T48, T72 and T96, respectively), and eight and sixteen days post-transfusion (T8d and T16d, respectively). The blood bags stored for 35 days showed an increase in K, pCO2, pO2, lactate, plasma hemoglobin and decreased plasma pH, sodium and leukocytes. The sheep transfused from all groups had increased GV, red cell count and total hemoglobin in the T30. The animals of the G35 had higher plasma hemoglobin in T12, a significant decrease in blood pH indicating a mild metabolic acidosis on T96. With respect to oxidative stress, was observed a decreased on catalase values of the G35 at T30, T6, T12 and T24, suggesting the occurrence of hemolysis witch was supported by the concomitant increase in the total bilirubin values at the same periods. Animals that received blood stored for 35 days showed higher alteration on hematological, blood gas, biochemical and oxidative stress parameters |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-07-04 2013-12-02 2016-08-15T20:31:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SOUSA, Rejane dos Santos. Evaluation of homologous blood transfusion in sheep. 2012. 169 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2012. https://repositorio.ufersa.edu.br/handle/tede/334 |
identifier_str_mv |
SOUSA, Rejane dos Santos. Evaluation of homologous blood transfusion in sheep. 2012. 169 f. Dissertação (Mestrado em Sanidade e Produção Animal) - Universidade Federal Rural do Semi-Árido, Mossoró, 2012. |
url |
https://repositorio.ufersa.edu.br/handle/tede/334 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido BR Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido BR Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
dc.source.none.fl_str_mv |
reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
instname_str |
Universidade Federal Rural do Semi-Árido (UFERSA) |
instacron_str |
UFERSA |
institution |
UFERSA |
reponame_str |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
collection |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
repository.name.fl_str_mv |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br |
_version_ |
1809747470271381504 |