Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
Texto Completo: | https://repositorio.ufersa.edu.br/handle/prefix/6731 |
Resumo: | The purpose of this study was to evaluate the antimicrobial activity of the geopropolis extract of the Jandaíra bee (Melipona subnitida Ducke) on Salmonela sp. isolated from beef and chicken sold in the city of Mossoró/RN as an alternative to antibiotics. To conduct the experiments, samples were acquired in the form of a consumer in 30 establishments throughout the city. Sixty samples were collected, 30 of beef (soft drumstick) and 30 of chicken (breast). For detection of Salmonella sp. the procedures described in normative instruction n° 62 of 2003 were followed. Positive samples were submitted to two tests, both using the disk diffusion method. In the first, the susceptibility to antimicrobials for clinical use was evaluated and in the second, the samples were submitted to the extract of geopropolis from the Jandaíra bee, diluted in three different concentrations (100%, 75% and 50%) in order to assess their potential antimicrobial, halos ≥ 6 mm were considered positive. As a result, 18 samples were positive for Salmonella sp., 12 (20%) from chicken and 6 (10%) from beef. In the evaluation of clinical antibiotics, the results showed a resistance index of 72% (13/18) to ampicillin, 11% (2/18) to sulfamethoxazole/trimethoprim, cefoxitin and amoxicillin/clavulanic acid and 6% (1/18) ) cefotaxime. 100% were sensitive to amikacin, meropenem and chloraphenicol, 94% (17/18) to ciproflaxaxin, 89% (16/18) to sulfamethoxazole/trimethoprim, cefoxitin, cefotaxime and clavulanic amoxicillin, 83% to ceftazidime and cefepime and 28 % (5/18) to ampicillin, showed an intermediate reaction to cefepime and ceftazidime 17% (3/18) and ciprofloxaxin and cefotaxime 6% (1/18). And as a response to the geopropolis extract, in the dilutions of 100% and 75%, the halos measured an average of 8mm and in 50% they had a measure of 9mm. It is concluded that there is a need for more attention in the quality control of these meats, since the presence of Salmonella sp. in 30% (18/60) of the samples analyzed, as well as their resistance to some antibiotics tested. On the other hand, we verified that the geopropolis extract presented itself as an effective antibacterial alternative |
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Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frangoProblema de saúde públicaResistência a antibióticosExtrato de geoprópolisPublic health problemAntibiotic resistanceGeopropolis extractCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThe purpose of this study was to evaluate the antimicrobial activity of the geopropolis extract of the Jandaíra bee (Melipona subnitida Ducke) on Salmonela sp. isolated from beef and chicken sold in the city of Mossoró/RN as an alternative to antibiotics. To conduct the experiments, samples were acquired in the form of a consumer in 30 establishments throughout the city. Sixty samples were collected, 30 of beef (soft drumstick) and 30 of chicken (breast). For detection of Salmonella sp. the procedures described in normative instruction n° 62 of 2003 were followed. Positive samples were submitted to two tests, both using the disk diffusion method. In the first, the susceptibility to antimicrobials for clinical use was evaluated and in the second, the samples were submitted to the extract of geopropolis from the Jandaíra bee, diluted in three different concentrations (100%, 75% and 50%) in order to assess their potential antimicrobial, halos ≥ 6 mm were considered positive. As a result, 18 samples were positive for Salmonella sp., 12 (20%) from chicken and 6 (10%) from beef. In the evaluation of clinical antibiotics, the results showed a resistance index of 72% (13/18) to ampicillin, 11% (2/18) to sulfamethoxazole/trimethoprim, cefoxitin and amoxicillin/clavulanic acid and 6% (1/18) ) cefotaxime. 100% were sensitive to amikacin, meropenem and chloraphenicol, 94% (17/18) to ciproflaxaxin, 89% (16/18) to sulfamethoxazole/trimethoprim, cefoxitin, cefotaxime and clavulanic amoxicillin, 83% to ceftazidime and cefepime and 28 % (5/18) to ampicillin, showed an intermediate reaction to cefepime and ceftazidime 17% (3/18) and ciprofloxaxin and cefotaxime 6% (1/18). And as a response to the geopropolis extract, in the dilutions of 100% and 75%, the halos measured an average of 8mm and in 50% they had a measure of 9mm. It is concluded that there is a need for more attention in the quality control of these meats, since the presence of Salmonella sp. in 30% (18/60) of the samples analyzed, as well as their resistance to some antibiotics tested. On the other hand, we verified that the geopropolis extract presented itself as an effective antibacterial alternativeO proposito desse estudo foi avaliar a atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (Melipona subnitida Ducke) em Salmonela sp. isoladas de carne bovina e frango comercializadas no Município de Mossoró/RN como alternativa aos antibióticos. Para a condução dos experimentos as amostras foram adquiridas na forma de consumidor em 30 estabelecimentos espalhados pela cidade. Foram coletadas 60 amostras, sendo 30 de carne bovina (coxão mole) e 30 de frango (peito). Para detecção de Salmonela sp. seguiu-se os procedimentos descritos na instrução normativa n° 62 de 2003. Amostras positivas foram submetidas a dois testes, ambos usando o método de difusão a disco. No primeiro, foi avaliada a susceptibilidade aos antimicrobianos de uso clínico e no segundo, as amostras foram submetidas ao extrato de geoprópolis da abelha jandaíra, diluídos em três concentrações diferentes (100%, 75% e 50%) a fim de avaliar o seu potencial antimicrobiano, foram considerados positivos halos ≥ 6 mm. Como resultado obteve-se 18 amostras positivas para Salmonela sp., 12 (20%) de frango e 6 (10%) de carne bovina. Na avaliação aos antibióticos de uso clinico os resultados demostraram um índice de resistência de 72% (13/18) a ampicilina, 11% (2/18) a sulfametoxazol/trimetropim, cefoxitina e amoxicilina/ácido clavulânico e 6% (1/18) a cefotaxima. 100% apresentaram-se sensíveis a amicacina, meropenem e clorafenicol, 94% (17/18) a ciproflaxaxino, 89% (16/18) a sulfametoxazol/trimetropim, cefoxitina, cefotaxima e amoxicilina clavulânico, 83% a ceftazidima e cefepima e 28% (5/18) a ampicilina, apresentaram ainda reação intermediária a cefepima e ceftazidima 17% (3/18) e ciprofloxaxino e cefotaxima 6% (1/18). E como resposta ao extrato de geoprópolis, nas diluições de 100% e 75% os halos mediram em média 8mm e em 50% apresentaram medida de 9mm. Conclui-se que há necessidade de mais atenção no controle de qualidade dessas carnes, visto que foi verificado presença de Salmonela sp. em 30% (18/60) das amostras analisadas, bem como resistência das mesmas a alguns antibióticos testados. Em contrapartida verificamos que o extrato de geoprópolis apresentou-se como uma alternativa antibacteriana eficazCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESUniversidade Federal Rural do Semi-ÁridoBrasilCentro de Ciências Agrárias - CCAUFERSAPrograma de Pós-Graduação em Ciência AnimalAires, Caio Augusto Martins94768242200http://lattes.cnpq.br/2927115194581869Silva, Jean Berg Alves da02556429461http://lattes.cnpq.br/1849041497210600Silva, Jean Berg Alves da02556429461http://lattes.cnpq.br/1849041497210600Aires, Caio Augusto Martins94768242200http://lattes.cnpq.br/2927115194581869Abrantes, Maria Rociene05476448411http://lattes.cnpq.br/8581676690083096Silva, Érica Lorenna Batista da2021-12-09T18:47:45Z2021-06-242021-12-09T18:47:45Z2021-03-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfSilva (2021) (SILVA, 2021)https://repositorio.ufersa.edu.br/handle/prefix/6731porSILVA, Erica Lorenna Batista da. Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango. 2021. 38 f. Dissertação (Mestrado em Ciência Animal), Universidade Federal Rural do Semi-Árido, Mossoró, 2021.CC-BY-SAhttps://creativecommons.org/licenses/by-sa/4.0/info:eu-repo/semantics/openAccessreponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU)instname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSA2023-10-30T20:28:07Zoai:repositorio.ufersa.edu.br:prefix/6731Repositório Institucionalhttps://repositorio.ufersa.edu.br/PUBhttps://repositorio.ufersa.edu.br/server/oai/requestrepositorio@ufersa.edu.br || admrepositorio@ufersa.edu.bropendoar:2023-10-30T20:28:07Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA)false |
dc.title.none.fl_str_mv |
Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango |
title |
Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango |
spellingShingle |
Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango Silva, Érica Lorenna Batista da Problema de saúde pública Resistência a antibióticos Extrato de geoprópolis Public health problem Antibiotic resistance Geopropolis extract CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango |
title_full |
Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango |
title_fullStr |
Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango |
title_full_unstemmed |
Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango |
title_sort |
Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango |
author |
Silva, Érica Lorenna Batista da |
author_facet |
Silva, Érica Lorenna Batista da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Aires, Caio Augusto Martins 94768242200 http://lattes.cnpq.br/2927115194581869 Silva, Jean Berg Alves da 02556429461 http://lattes.cnpq.br/1849041497210600 Silva, Jean Berg Alves da 02556429461 http://lattes.cnpq.br/1849041497210600 Aires, Caio Augusto Martins 94768242200 http://lattes.cnpq.br/2927115194581869 Abrantes, Maria Rociene 05476448411 http://lattes.cnpq.br/8581676690083096 |
dc.contributor.author.fl_str_mv |
Silva, Érica Lorenna Batista da |
dc.subject.por.fl_str_mv |
Problema de saúde pública Resistência a antibióticos Extrato de geoprópolis Public health problem Antibiotic resistance Geopropolis extract CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Problema de saúde pública Resistência a antibióticos Extrato de geoprópolis Public health problem Antibiotic resistance Geopropolis extract CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The purpose of this study was to evaluate the antimicrobial activity of the geopropolis extract of the Jandaíra bee (Melipona subnitida Ducke) on Salmonela sp. isolated from beef and chicken sold in the city of Mossoró/RN as an alternative to antibiotics. To conduct the experiments, samples were acquired in the form of a consumer in 30 establishments throughout the city. Sixty samples were collected, 30 of beef (soft drumstick) and 30 of chicken (breast). For detection of Salmonella sp. the procedures described in normative instruction n° 62 of 2003 were followed. Positive samples were submitted to two tests, both using the disk diffusion method. In the first, the susceptibility to antimicrobials for clinical use was evaluated and in the second, the samples were submitted to the extract of geopropolis from the Jandaíra bee, diluted in three different concentrations (100%, 75% and 50%) in order to assess their potential antimicrobial, halos ≥ 6 mm were considered positive. As a result, 18 samples were positive for Salmonella sp., 12 (20%) from chicken and 6 (10%) from beef. In the evaluation of clinical antibiotics, the results showed a resistance index of 72% (13/18) to ampicillin, 11% (2/18) to sulfamethoxazole/trimethoprim, cefoxitin and amoxicillin/clavulanic acid and 6% (1/18) ) cefotaxime. 100% were sensitive to amikacin, meropenem and chloraphenicol, 94% (17/18) to ciproflaxaxin, 89% (16/18) to sulfamethoxazole/trimethoprim, cefoxitin, cefotaxime and clavulanic amoxicillin, 83% to ceftazidime and cefepime and 28 % (5/18) to ampicillin, showed an intermediate reaction to cefepime and ceftazidime 17% (3/18) and ciprofloxaxin and cefotaxime 6% (1/18). And as a response to the geopropolis extract, in the dilutions of 100% and 75%, the halos measured an average of 8mm and in 50% they had a measure of 9mm. It is concluded that there is a need for more attention in the quality control of these meats, since the presence of Salmonella sp. in 30% (18/60) of the samples analyzed, as well as their resistance to some antibiotics tested. On the other hand, we verified that the geopropolis extract presented itself as an effective antibacterial alternative |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-09T18:47:45Z 2021-06-24 2021-12-09T18:47:45Z 2021-03-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Silva (2021) (SILVA, 2021) https://repositorio.ufersa.edu.br/handle/prefix/6731 |
identifier_str_mv |
Silva (2021) (SILVA, 2021) |
url |
https://repositorio.ufersa.edu.br/handle/prefix/6731 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
SILVA, Erica Lorenna Batista da. Atividade antimicrobiana do extrato de geoprópolis da abelha jandaíra (melipona subnitida ducke) em salmonela sp. Isoladas de carne bovina e frango. 2021. 38 f. Dissertação (Mestrado em Ciência Animal), Universidade Federal Rural do Semi-Árido, Mossoró, 2021. |
dc.rights.driver.fl_str_mv |
CC-BY-SA https://creativecommons.org/licenses/by-sa/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA https://creativecommons.org/licenses/by-sa/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido Brasil Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
publisher.none.fl_str_mv |
Universidade Federal Rural do Semi-Árido Brasil Centro de Ciências Agrárias - CCA UFERSA Programa de Pós-Graduação em Ciência Animal |
dc.source.none.fl_str_mv |
reponame:Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) instname:Universidade Federal Rural do Semi-Árido (UFERSA) instacron:UFERSA |
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Universidade Federal Rural do Semi-Árido (UFERSA) |
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UFERSA |
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UFERSA |
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Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
collection |
Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) |
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Repositório Digital da Universidade Federal Rural do Semi-Árido (RDU) - Universidade Federal Rural do Semi-Árido (UFERSA) |
repository.mail.fl_str_mv |
repositorio@ufersa.edu.br || admrepositorio@ufersa.edu.br |
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1809747473643601920 |