Efeito do sildenafil na função endotelial de camundongos ateroscleróticos
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) |
Texto Completo: | http://repositorio.ufes.br/handle/10/8005 |
Resumo: | In previous studies we have shown that sildenafil ameliorates aortic relaxations in apolipoprotein E knockout (apoE) mice. Now, we tested the hypothesis that endothelial dysfunction (ED) in this model is characterized by contractile hyperresponsiveness to phenylephrine (PE) and that this abnormality could be repaired by the treatment with sildenafil. The aortic rings were evaluated in apoE mice treated with sildenafil (apoE-sil, 40 mg/kg/day) and compared with apoE and wild-type (WT) mice administered with vehicle (veh). The apoE-veh mice exhibited an imbalance of nitric oxide and reactive oxygen species (NO/ROS) levels and an increased maximum response (Rmax, 20%) and sensitivity (7%) to PE, which were not modified by endothelial removal. Under the prostanoids blockade, vasocontraction was decreased more in apoE-veh (-37%) than in WT (-27%) and apoE-sil (-30%) mice. NADPH-oxidase blockade abolished the enhanced contractile responsiveness in apoE-veh (-33%), without effects in WT and apoE-sil groups. The atherosclerotic lesions and the imbalance of NO/ROS were reduced (40%) in apoEsil mice. In conclusion, ED in apoE mice was characterized by decreased NObioavailability and contractile hyperresponsiveness, due to thromboxane and oxidative stress, and was normalized by sildenafil. The beneficial effects of this phosphodiesterase-5 inhibitor on ED and lipid deposition provide new insights for its use as adjuvant in the treatment of atherosclerosis. |
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Balarini, Camille de MouraVasquez, Elisardo CorralLeal, Marcos André SoaresPereira, Thiago de Melo CostaGraceli, Jones Bernardes2018-08-01T22:58:48Z2018-08-012018-08-01T22:58:48Z2015-06-02In previous studies we have shown that sildenafil ameliorates aortic relaxations in apolipoprotein E knockout (apoE) mice. Now, we tested the hypothesis that endothelial dysfunction (ED) in this model is characterized by contractile hyperresponsiveness to phenylephrine (PE) and that this abnormality could be repaired by the treatment with sildenafil. The aortic rings were evaluated in apoE mice treated with sildenafil (apoE-sil, 40 mg/kg/day) and compared with apoE and wild-type (WT) mice administered with vehicle (veh). The apoE-veh mice exhibited an imbalance of nitric oxide and reactive oxygen species (NO/ROS) levels and an increased maximum response (Rmax, 20%) and sensitivity (7%) to PE, which were not modified by endothelial removal. Under the prostanoids blockade, vasocontraction was decreased more in apoE-veh (-37%) than in WT (-27%) and apoE-sil (-30%) mice. NADPH-oxidase blockade abolished the enhanced contractile responsiveness in apoE-veh (-33%), without effects in WT and apoE-sil groups. The atherosclerotic lesions and the imbalance of NO/ROS were reduced (40%) in apoEsil mice. In conclusion, ED in apoE mice was characterized by decreased NObioavailability and contractile hyperresponsiveness, due to thromboxane and oxidative stress, and was normalized by sildenafil. The beneficial effects of this phosphodiesterase-5 inhibitor on ED and lipid deposition provide new insights for its use as adjuvant in the treatment of atherosclerosis.Vimos anteriormente que o sildenafil restaura o relaxamento dependente do endotélio em aortas de animais ateroscleróticos apolipoprotein E knockout (apoE). No presente estudo, testamos a hipótese que a disfunção endotelial tambem é caracterizada por hiper-reatividade à fenilefrina (FE), e que o sildenafil pode reparar esta anormalidade neste modelo experimental. Anéis de aorta de camundongos apoE tratados com sildenafil (apoE-sil, 40 mg/kg/dia) foram avaliados e comparados com apoE-veí e controle (CT) os quais receberam apenas veículo. O grupo apoE-veí exibiu um desbalanço entre os os níveis de Óxido Nítrico (NO) e Espécies Reativas de Óxigênio (NO/EROs) e um aumento na resposta máxIma (Rmax, 20%) e sensibilidade (7%) à FE, os quais não foram modificados após retirada do endotélio. Após o bloqueio com indometacina a resposta máxima diminuiu sigficativamente no grupo apoE-veí (37%), no grupo CT (27%), e no grupo apoE-sil (30%). Em seguida, bloqueamos a NADPH Oxidase, e a resposta máxima diminuiu sigficativamente no grupo apoE-/- veículo (- 33%), enquanto nos demais grupos não foi observada diferença. O sildenafil reduziu as lesões ateroscleróticas e o desbalanço (NO/EROs) nos camundongos apoE (40%). Concluímos portanto, que a disfunção endotelial no camudongos apoE-veí foi caracterizada por diminuição na biodisponibilidade de NO, e aumento da resposta contrátil à FE, devido ao estresse oxidativo e tromboxano A2, e o sildenafil corrigiu esta anormalidade. Os efeitos benéficos deste inibidor da fosfodiesterase-5 na disfunção endotelial e deposição lipídica o levam a ser uma interessante ferramenta para o tratamento da aterosclerose.Texthttp://repositorio.ufes.br/handle/10/8005porUniversidade Federal do Espírito SantoMestrado em Ciências FisiológicasPrograma de Pós-Graduação em Ciências FisiológicasUFESBRCentro de Ciências da SaúdeReatividade vascularAnéis de aortaAteroscleroseCamundongos ApoE KnockoutSildenafilÓxido nítricoEstresse oxidativoDisfunção endotelialGMP cíclicoFosfodiesterase 5Fisiologia612Efeito do sildenafil na função endotelial de camundongos ateroscleróticosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALtese_8988_Dissertação Marcos André Soares Leal.pdfapplication/pdf1544945http://repositorio.ufes.br/bitstreams/089cb566-3206-4e59-9fb2-5606cf663486/download5d7d84076b861c354eae9912af1bc606MD5110/80052024-07-16 17:10:17.801oai:repositorio.ufes.br:10/8005http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-10-15T18:01:22.938278Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false |
dc.title.none.fl_str_mv |
Efeito do sildenafil na função endotelial de camundongos ateroscleróticos |
title |
Efeito do sildenafil na função endotelial de camundongos ateroscleróticos |
spellingShingle |
Efeito do sildenafil na função endotelial de camundongos ateroscleróticos Leal, Marcos André Soares Reatividade vascular Anéis de aorta Aterosclerose Camundongos ApoE Knockout Sildenafil Óxido nítrico Estresse oxidativo Disfunção endotelial GMP cíclico Fosfodiesterase 5 Fisiologia 612 |
title_short |
Efeito do sildenafil na função endotelial de camundongos ateroscleróticos |
title_full |
Efeito do sildenafil na função endotelial de camundongos ateroscleróticos |
title_fullStr |
Efeito do sildenafil na função endotelial de camundongos ateroscleróticos |
title_full_unstemmed |
Efeito do sildenafil na função endotelial de camundongos ateroscleróticos |
title_sort |
Efeito do sildenafil na função endotelial de camundongos ateroscleróticos |
author |
Leal, Marcos André Soares |
author_facet |
Leal, Marcos André Soares |
author_role |
author |
dc.contributor.advisor-co1.fl_str_mv |
Balarini, Camille de Moura |
dc.contributor.advisor1.fl_str_mv |
Vasquez, Elisardo Corral |
dc.contributor.author.fl_str_mv |
Leal, Marcos André Soares |
dc.contributor.referee1.fl_str_mv |
Pereira, Thiago de Melo Costa |
dc.contributor.referee2.fl_str_mv |
Graceli, Jones Bernardes |
contributor_str_mv |
Balarini, Camille de Moura Vasquez, Elisardo Corral Pereira, Thiago de Melo Costa Graceli, Jones Bernardes |
dc.subject.por.fl_str_mv |
Reatividade vascular Anéis de aorta Aterosclerose Camundongos ApoE Knockout Sildenafil Óxido nítrico Estresse oxidativo Disfunção endotelial GMP cíclico Fosfodiesterase 5 |
topic |
Reatividade vascular Anéis de aorta Aterosclerose Camundongos ApoE Knockout Sildenafil Óxido nítrico Estresse oxidativo Disfunção endotelial GMP cíclico Fosfodiesterase 5 Fisiologia 612 |
dc.subject.cnpq.fl_str_mv |
Fisiologia |
dc.subject.udc.none.fl_str_mv |
612 |
description |
In previous studies we have shown that sildenafil ameliorates aortic relaxations in apolipoprotein E knockout (apoE) mice. Now, we tested the hypothesis that endothelial dysfunction (ED) in this model is characterized by contractile hyperresponsiveness to phenylephrine (PE) and that this abnormality could be repaired by the treatment with sildenafil. The aortic rings were evaluated in apoE mice treated with sildenafil (apoE-sil, 40 mg/kg/day) and compared with apoE and wild-type (WT) mice administered with vehicle (veh). The apoE-veh mice exhibited an imbalance of nitric oxide and reactive oxygen species (NO/ROS) levels and an increased maximum response (Rmax, 20%) and sensitivity (7%) to PE, which were not modified by endothelial removal. Under the prostanoids blockade, vasocontraction was decreased more in apoE-veh (-37%) than in WT (-27%) and apoE-sil (-30%) mice. NADPH-oxidase blockade abolished the enhanced contractile responsiveness in apoE-veh (-33%), without effects in WT and apoE-sil groups. The atherosclerotic lesions and the imbalance of NO/ROS were reduced (40%) in apoEsil mice. In conclusion, ED in apoE mice was characterized by decreased NObioavailability and contractile hyperresponsiveness, due to thromboxane and oxidative stress, and was normalized by sildenafil. The beneficial effects of this phosphodiesterase-5 inhibitor on ED and lipid deposition provide new insights for its use as adjuvant in the treatment of atherosclerosis. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-06-02 |
dc.date.accessioned.fl_str_mv |
2018-08-01T22:58:48Z |
dc.date.available.fl_str_mv |
2018-08-01 2018-08-01T22:58:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufes.br/handle/10/8005 |
url |
http://repositorio.ufes.br/handle/10/8005 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
Text |
dc.publisher.none.fl_str_mv |
Universidade Federal do Espírito Santo Mestrado em Ciências Fisiológicas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Fisiológicas |
dc.publisher.initials.fl_str_mv |
UFES |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Federal do Espírito Santo Mestrado em Ciências Fisiológicas |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) instname:Universidade Federal do Espírito Santo (UFES) instacron:UFES |
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Universidade Federal do Espírito Santo (UFES) |
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UFES |
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UFES |
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Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) |
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