Efeito do sildenafil na função endotelial de camundongos ateroscleróticos

Detalhes bibliográficos
Autor(a) principal: Leal, Marcos André Soares
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/8005
Resumo: In previous studies we have shown that sildenafil ameliorates aortic relaxations in apolipoprotein E knockout (apoE) mice. Now, we tested the hypothesis that endothelial dysfunction (ED) in this model is characterized by contractile hyperresponsiveness to phenylephrine (PE) and that this abnormality could be repaired by the treatment with sildenafil. The aortic rings were evaluated in apoE mice treated with sildenafil (apoE-sil, 40 mg/kg/day) and compared with apoE and wild-type (WT) mice administered with vehicle (veh). The apoE-veh mice exhibited an imbalance of nitric oxide and reactive oxygen species (NO/ROS) levels and an increased maximum response (Rmax, 20%) and sensitivity (7%) to PE, which were not modified by endothelial removal. Under the prostanoids blockade, vasocontraction was decreased more in apoE-veh (-37%) than in WT (-27%) and apoE-sil (-30%) mice. NADPH-oxidase blockade abolished the enhanced contractile responsiveness in apoE-veh (-33%), without effects in WT and apoE-sil groups. The atherosclerotic lesions and the imbalance of NO/ROS were reduced (40%) in apoEsil mice. In conclusion, ED in apoE mice was characterized by decreased NObioavailability and contractile hyperresponsiveness, due to thromboxane and oxidative stress, and was normalized by sildenafil. The beneficial effects of this phosphodiesterase-5 inhibitor on ED and lipid deposition provide new insights for its use as adjuvant in the treatment of atherosclerosis.
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spelling Balarini, Camille de MouraVasquez, Elisardo CorralLeal, Marcos André SoaresPereira, Thiago de Melo CostaGraceli, Jones Bernardes2018-08-01T22:58:48Z2018-08-012018-08-01T22:58:48Z2015-06-02In previous studies we have shown that sildenafil ameliorates aortic relaxations in apolipoprotein E knockout (apoE) mice. Now, we tested the hypothesis that endothelial dysfunction (ED) in this model is characterized by contractile hyperresponsiveness to phenylephrine (PE) and that this abnormality could be repaired by the treatment with sildenafil. The aortic rings were evaluated in apoE mice treated with sildenafil (apoE-sil, 40 mg/kg/day) and compared with apoE and wild-type (WT) mice administered with vehicle (veh). The apoE-veh mice exhibited an imbalance of nitric oxide and reactive oxygen species (NO/ROS) levels and an increased maximum response (Rmax, 20%) and sensitivity (7%) to PE, which were not modified by endothelial removal. Under the prostanoids blockade, vasocontraction was decreased more in apoE-veh (-37%) than in WT (-27%) and apoE-sil (-30%) mice. NADPH-oxidase blockade abolished the enhanced contractile responsiveness in apoE-veh (-33%), without effects in WT and apoE-sil groups. The atherosclerotic lesions and the imbalance of NO/ROS were reduced (40%) in apoEsil mice. In conclusion, ED in apoE mice was characterized by decreased NObioavailability and contractile hyperresponsiveness, due to thromboxane and oxidative stress, and was normalized by sildenafil. The beneficial effects of this phosphodiesterase-5 inhibitor on ED and lipid deposition provide new insights for its use as adjuvant in the treatment of atherosclerosis.Vimos anteriormente que o sildenafil restaura o relaxamento dependente do endotélio em aortas de animais ateroscleróticos apolipoprotein E knockout (apoE). No presente estudo, testamos a hipótese que a disfunção endotelial tambem é caracterizada por hiper-reatividade à fenilefrina (FE), e que o sildenafil pode reparar esta anormalidade neste modelo experimental. Anéis de aorta de camundongos apoE tratados com sildenafil (apoE-sil, 40 mg/kg/dia) foram avaliados e comparados com apoE-veí e controle (CT) os quais receberam apenas veículo. O grupo apoE-veí exibiu um desbalanço entre os os níveis de Óxido Nítrico (NO) e Espécies Reativas de Óxigênio (NO/EROs) e um aumento na resposta máxIma (Rmax, 20%) e sensibilidade (7%) à FE, os quais não foram modificados após retirada do endotélio. Após o bloqueio com indometacina a resposta máxima diminuiu sigficativamente no grupo apoE-veí (37%), no grupo CT (27%), e no grupo apoE-sil (30%). Em seguida, bloqueamos a NADPH Oxidase, e a resposta máxima diminuiu sigficativamente no grupo apoE-/- veículo (- 33%), enquanto nos demais grupos não foi observada diferença. O sildenafil reduziu as lesões ateroscleróticas e o desbalanço (NO/EROs) nos camundongos apoE (40%). Concluímos portanto, que a disfunção endotelial no camudongos apoE-veí foi caracterizada por diminuição na biodisponibilidade de NO, e aumento da resposta contrátil à FE, devido ao estresse oxidativo e tromboxano A2, e o sildenafil corrigiu esta anormalidade. Os efeitos benéficos deste inibidor da fosfodiesterase-5 na disfunção endotelial e deposição lipídica o levam a ser uma interessante ferramenta para o tratamento da aterosclerose.Texthttp://repositorio.ufes.br/handle/10/8005porUniversidade Federal do Espírito SantoMestrado em Ciências FisiológicasPrograma de Pós-Graduação em Ciências FisiológicasUFESBRCentro de Ciências da SaúdeReatividade vascularAnéis de aortaAteroscleroseCamundongos ApoE KnockoutSildenafilÓxido nítricoEstresse oxidativoDisfunção endotelialGMP cíclicoFosfodiesterase 5Fisiologia612Efeito do sildenafil na função endotelial de camundongos ateroscleróticosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALtese_8988_Dissertação Marcos André Soares Leal.pdfapplication/pdf1544945http://repositorio.ufes.br/bitstreams/089cb566-3206-4e59-9fb2-5606cf663486/download5d7d84076b861c354eae9912af1bc606MD5110/80052024-07-16 17:10:17.801oai:repositorio.ufes.br:10/8005http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-10-15T18:01:22.938278Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Efeito do sildenafil na função endotelial de camundongos ateroscleróticos
title Efeito do sildenafil na função endotelial de camundongos ateroscleróticos
spellingShingle Efeito do sildenafil na função endotelial de camundongos ateroscleróticos
Leal, Marcos André Soares
Reatividade vascular
Anéis de aorta
Aterosclerose
Camundongos ApoE Knockout
Sildenafil
Óxido nítrico
Estresse oxidativo
Disfunção endotelial
GMP cíclico
Fosfodiesterase 5
Fisiologia
612
title_short Efeito do sildenafil na função endotelial de camundongos ateroscleróticos
title_full Efeito do sildenafil na função endotelial de camundongos ateroscleróticos
title_fullStr Efeito do sildenafil na função endotelial de camundongos ateroscleróticos
title_full_unstemmed Efeito do sildenafil na função endotelial de camundongos ateroscleróticos
title_sort Efeito do sildenafil na função endotelial de camundongos ateroscleróticos
author Leal, Marcos André Soares
author_facet Leal, Marcos André Soares
author_role author
dc.contributor.advisor-co1.fl_str_mv Balarini, Camille de Moura
dc.contributor.advisor1.fl_str_mv Vasquez, Elisardo Corral
dc.contributor.author.fl_str_mv Leal, Marcos André Soares
dc.contributor.referee1.fl_str_mv Pereira, Thiago de Melo Costa
dc.contributor.referee2.fl_str_mv Graceli, Jones Bernardes
contributor_str_mv Balarini, Camille de Moura
Vasquez, Elisardo Corral
Pereira, Thiago de Melo Costa
Graceli, Jones Bernardes
dc.subject.por.fl_str_mv Reatividade vascular
Anéis de aorta
Aterosclerose
Camundongos ApoE Knockout
Sildenafil
Óxido nítrico
Estresse oxidativo
Disfunção endotelial
GMP cíclico
Fosfodiesterase 5
topic Reatividade vascular
Anéis de aorta
Aterosclerose
Camundongos ApoE Knockout
Sildenafil
Óxido nítrico
Estresse oxidativo
Disfunção endotelial
GMP cíclico
Fosfodiesterase 5
Fisiologia
612
dc.subject.cnpq.fl_str_mv Fisiologia
dc.subject.udc.none.fl_str_mv 612
description In previous studies we have shown that sildenafil ameliorates aortic relaxations in apolipoprotein E knockout (apoE) mice. Now, we tested the hypothesis that endothelial dysfunction (ED) in this model is characterized by contractile hyperresponsiveness to phenylephrine (PE) and that this abnormality could be repaired by the treatment with sildenafil. The aortic rings were evaluated in apoE mice treated with sildenafil (apoE-sil, 40 mg/kg/day) and compared with apoE and wild-type (WT) mice administered with vehicle (veh). The apoE-veh mice exhibited an imbalance of nitric oxide and reactive oxygen species (NO/ROS) levels and an increased maximum response (Rmax, 20%) and sensitivity (7%) to PE, which were not modified by endothelial removal. Under the prostanoids blockade, vasocontraction was decreased more in apoE-veh (-37%) than in WT (-27%) and apoE-sil (-30%) mice. NADPH-oxidase blockade abolished the enhanced contractile responsiveness in apoE-veh (-33%), without effects in WT and apoE-sil groups. The atherosclerotic lesions and the imbalance of NO/ROS were reduced (40%) in apoEsil mice. In conclusion, ED in apoE mice was characterized by decreased NObioavailability and contractile hyperresponsiveness, due to thromboxane and oxidative stress, and was normalized by sildenafil. The beneficial effects of this phosphodiesterase-5 inhibitor on ED and lipid deposition provide new insights for its use as adjuvant in the treatment of atherosclerosis.
publishDate 2015
dc.date.issued.fl_str_mv 2015-06-02
dc.date.accessioned.fl_str_mv 2018-08-01T22:58:48Z
dc.date.available.fl_str_mv 2018-08-01
2018-08-01T22:58:48Z
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dc.language.iso.fl_str_mv por
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dc.format.none.fl_str_mv Text
dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciências Fisiológicas
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Fisiológicas
dc.publisher.initials.fl_str_mv UFES
dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Ciências Fisiológicas
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