Avaliação do potencial osteointegrativo de biomaterial ósseo

Detalhes bibliográficos
Autor(a) principal: Gusman, Ruy Rocha
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)
Texto Completo: http://repositorio.ufes.br/handle/10/13302
Resumo: The growing need for the use of bone grafts, mainly related to orthopedic and dental surgery, is generating the need for production of new biomaterials. Given this need, the use of xenograft as been an alternative as biomaterial. The use of younger bone grafts, which provide primitive factors involved in bone development, could induce more efficient bone regeneration, which can be found using fetal bone grafts. The objective of this study was to test the osteointegration of a decellularized bone biomaterial produced from 0,5% SDS trated bovine fetal bone for use in tissuebio-engineering. To test osteointegration, grafts were applied to cranial holes in rats. Subsequently histochemical stain in gand optical microscopy techniques were used, as well as quantitative collagene valuation by Image J softwere. There was no statistical differences between the different groups evaluated, regardless of the type of graft used (FC: Celularized Fetal - Control, FD: Decellularized Fetal, AC: Celularized Adult – Control, AD: Decellularized Adult) and the animals eutanásia time (2 or 4 weeks). Even for animals that did not recived graft (VZ: Void), there was no statistical significance for the same euthanasia periods mentioned. However, the qualitative evaluation by optical microscopy showed an arrangement to collagen fibers in the longitudinal and parallel direction, joing there main bones (where the holes were made in the skull of the animals), and also the formation of cartilage in the cranial holes in the decellularized groups. It seems to show a potencial of SDS 0,5% treated xenograft to function as biomaterial in the future. New evaluations will be required to confirm this potencial.
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spelling Nogueira, Breno Valentimhttps://orcid.org/0000000221990635http://lattes.cnpq.br/0011229320439147Gusman, Ruy Rochahttps://orcid.org/0000-0002-5984-633Xhttp://lattes.cnpq.br/0413143493263436Graceli, Jones Bernardeshttps://orcid.org/0000000193494369http://lattes.cnpq.br/3803670746263603Balarini, Camille de Mourahttps://orcid.org/0000-0002-4648-6443http://lattes.cnpq.br/0891042053942023Paula, Flavia dehttps://orcid.org/0000000186792982http://lattes.cnpq.br/79132014506636832024-05-29T22:10:53Z2024-05-29T22:10:53Z2019-09-23The growing need for the use of bone grafts, mainly related to orthopedic and dental surgery, is generating the need for production of new biomaterials. Given this need, the use of xenograft as been an alternative as biomaterial. The use of younger bone grafts, which provide primitive factors involved in bone development, could induce more efficient bone regeneration, which can be found using fetal bone grafts. The objective of this study was to test the osteointegration of a decellularized bone biomaterial produced from 0,5% SDS trated bovine fetal bone for use in tissuebio-engineering. To test osteointegration, grafts were applied to cranial holes in rats. Subsequently histochemical stain in gand optical microscopy techniques were used, as well as quantitative collagene valuation by Image J softwere. There was no statistical differences between the different groups evaluated, regardless of the type of graft used (FC: Celularized Fetal - Control, FD: Decellularized Fetal, AC: Celularized Adult – Control, AD: Decellularized Adult) and the animals eutanásia time (2 or 4 weeks). Even for animals that did not recived graft (VZ: Void), there was no statistical significance for the same euthanasia periods mentioned. However, the qualitative evaluation by optical microscopy showed an arrangement to collagen fibers in the longitudinal and parallel direction, joing there main bones (where the holes were made in the skull of the animals), and also the formation of cartilage in the cranial holes in the decellularized groups. It seems to show a potencial of SDS 0,5% treated xenograft to function as biomaterial in the future. New evaluations will be required to confirm this potencial.A necessidade crescente de utilização de enxertos ósseos, principalmente relacionados com as cirurgia ortopédicas e odontológicas, está gerando a necessidade da produção de novos biomateriais. Diante desta necessidade o emprego de xenoenxerto tem sido uma alternativa como biomaterial. A utilização de enxerto ósseo mais jovens, que forneçam fatores primitivos envolvidos no desenvolvimento ósseo, poderiam induzir uma regeneração óssea mais eficiente, o que pode ser encontrado utilizando-se enxerto ósseo de origem fetal. Objetivou-se testar a osteointegração de um biomaterial ósseo descelularizado produzido a partir de osso fetal bovino tratado com SDS 0,5% para utilização em biogenharia tecidual. Para testar a osteointegração foi realizada a aplicação de enxertos em orifícios craniais em ratos. Posteriormente foram utilizadas técnicas de coloração histoquímica e de microscopia óptica, bem como a avaliação quantitativa do colágeno pelo programa ImageJ. Não houve diferenças estatísticas entre os diferentes grupos avaliados, independentemente do tipo de enxerto utilizado (FC : Fetal controle ou celularizado, FD : Fetal descelularizado, AC : Adulto controle ou celularizado, AD : Adulto descelularizado), e do tempo de eutanásia dos animais (2 ou 4 semanas). Mesmo para os animais que não receberam enxerto (Vazio : VZ), não houve significância estatística para os mesmos períodos de eutanásia citados. Porém a avaliação qualitativa pela microscopia óptica demonstrou uma disposição das fibras colágenas no sentido longitudinal e paralelas fazendo a união entre os ossos remanescentes (no local onde foi realizado o orifício no crânio dos animais), e também a formação de cartilagem nos orifícios craniais dos grupos descelularizados. Issoparece demonstrar um potencial dos xenoenxertos tratados com SDS 0,5% para funcionarem como biomateriais no futuro. Novas avaliações serão necessárias para a confirmação deste potencial.Texthttp://repositorio.ufes.br/handle/10/13302porUniversidade Federal do Espírito SantoMestrado em BiotecnologiaPrograma de Pós-Graduação em BiotecnologiaUFESBRCentro de Ciências da Saúdesubject.br-rjbnBiotecnologiaOsso fetalEnxerto ósseoXenoenxertoBiomaterialOsteointegraçãoBonegraftFetal boneXenograftOsteointegrationAvaliação do potencial osteointegrativo de biomaterial ósseoEvaluation of osteointegrative potential of bone biomaterialinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal do Espírito Santo (riUfes)instname:Universidade Federal do Espírito Santo (UFES)instacron:UFESORIGINALDissertação_Ruy Rocha Gusman.pdfapplication/pdf2295097http://repositorio.ufes.br/bitstreams/760936af-53b5-4b85-8d8d-d3f69ac51ca1/download5d1200ffcea95a292d60d61e9c51dc09MD5110/133022024-08-14 14:49:34.693oai:repositorio.ufes.br:10/13302http://repositorio.ufes.brRepositório InstitucionalPUBhttp://repositorio.ufes.br/oai/requestopendoar:21082024-10-15T18:00:56.681600Repositório Institucional da Universidade Federal do Espírito Santo (riUfes) - Universidade Federal do Espírito Santo (UFES)false
dc.title.none.fl_str_mv Avaliação do potencial osteointegrativo de biomaterial ósseo
dc.title.alternative.none.fl_str_mv Evaluation of osteointegrative potential of bone biomaterial
title Avaliação do potencial osteointegrativo de biomaterial ósseo
spellingShingle Avaliação do potencial osteointegrativo de biomaterial ósseo
Gusman, Ruy Rocha
Biotecnologia
Osso fetal
Enxerto ósseo
Xenoenxerto
Biomaterial
Osteointegração
Bonegraft
Fetal bone
Xenograft
Osteointegration
subject.br-rjbn
title_short Avaliação do potencial osteointegrativo de biomaterial ósseo
title_full Avaliação do potencial osteointegrativo de biomaterial ósseo
title_fullStr Avaliação do potencial osteointegrativo de biomaterial ósseo
title_full_unstemmed Avaliação do potencial osteointegrativo de biomaterial ósseo
title_sort Avaliação do potencial osteointegrativo de biomaterial ósseo
author Gusman, Ruy Rocha
author_facet Gusman, Ruy Rocha
author_role author
dc.contributor.authorID.none.fl_str_mv https://orcid.org/0000-0002-5984-633X
dc.contributor.authorLattes.none.fl_str_mv http://lattes.cnpq.br/0413143493263436
dc.contributor.advisor1.fl_str_mv Nogueira, Breno Valentim
dc.contributor.advisor1ID.fl_str_mv https://orcid.org/0000000221990635
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/0011229320439147
dc.contributor.author.fl_str_mv Gusman, Ruy Rocha
dc.contributor.referee1.fl_str_mv Graceli, Jones Bernardes
dc.contributor.referee1ID.fl_str_mv https://orcid.org/0000000193494369
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/3803670746263603
dc.contributor.referee2.fl_str_mv Balarini, Camille de Moura
dc.contributor.referee2ID.fl_str_mv https://orcid.org/0000-0002-4648-6443
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/0891042053942023
dc.contributor.referee3.fl_str_mv Paula, Flavia de
dc.contributor.referee3ID.fl_str_mv https://orcid.org/0000000186792982
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/7913201450663683
contributor_str_mv Nogueira, Breno Valentim
Graceli, Jones Bernardes
Balarini, Camille de Moura
Paula, Flavia de
dc.subject.cnpq.fl_str_mv Biotecnologia
topic Biotecnologia
Osso fetal
Enxerto ósseo
Xenoenxerto
Biomaterial
Osteointegração
Bonegraft
Fetal bone
Xenograft
Osteointegration
subject.br-rjbn
dc.subject.por.fl_str_mv Osso fetal
Enxerto ósseo
Xenoenxerto
Biomaterial
Osteointegração
Bonegraft
Fetal bone
Xenograft
Osteointegration
dc.subject.br-rjbn.none.fl_str_mv subject.br-rjbn
description The growing need for the use of bone grafts, mainly related to orthopedic and dental surgery, is generating the need for production of new biomaterials. Given this need, the use of xenograft as been an alternative as biomaterial. The use of younger bone grafts, which provide primitive factors involved in bone development, could induce more efficient bone regeneration, which can be found using fetal bone grafts. The objective of this study was to test the osteointegration of a decellularized bone biomaterial produced from 0,5% SDS trated bovine fetal bone for use in tissuebio-engineering. To test osteointegration, grafts were applied to cranial holes in rats. Subsequently histochemical stain in gand optical microscopy techniques were used, as well as quantitative collagene valuation by Image J softwere. There was no statistical differences between the different groups evaluated, regardless of the type of graft used (FC: Celularized Fetal - Control, FD: Decellularized Fetal, AC: Celularized Adult – Control, AD: Decellularized Adult) and the animals eutanásia time (2 or 4 weeks). Even for animals that did not recived graft (VZ: Void), there was no statistical significance for the same euthanasia periods mentioned. However, the qualitative evaluation by optical microscopy showed an arrangement to collagen fibers in the longitudinal and parallel direction, joing there main bones (where the holes were made in the skull of the animals), and also the formation of cartilage in the cranial holes in the decellularized groups. It seems to show a potencial of SDS 0,5% treated xenograft to function as biomaterial in the future. New evaluations will be required to confirm this potencial.
publishDate 2019
dc.date.issued.fl_str_mv 2019-09-23
dc.date.accessioned.fl_str_mv 2024-05-29T22:10:53Z
dc.date.available.fl_str_mv 2024-05-29T22:10:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.format.none.fl_str_mv Text
dc.publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Biotecnologia
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Biotecnologia
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dc.publisher.country.fl_str_mv BR
dc.publisher.department.fl_str_mv Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Federal do Espírito Santo
Mestrado em Biotecnologia
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